Endoreduplication of the mouse genome in the absence of ORC1

In this study, Okano-Uchida et al. describe the physiological role of ORC1 in mice by generating knock-in mice with LoxP sites flanking exons encoding the critical ATPase domain of ORC1. They show that ORC1 ablation in extraembryonic trophoblasts and hepatocytes failed to impede genome endoreduplication and organ development and function and conclude that ORC1 in mice is essential for mitotic cell divisions but dispensable for endoreduplication.

• Publication year

  2018

• Venue

  Genes & Development

• Publication date

  2018-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1101/gad.311910.118PMID 29967292PMCID 6075035
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structure of the active form of human origin recognition complex and its ATPase motor module

  2017cited by this paper
• Two subunits of human ORC are dispensable for DNA replication and proliferation

  2016cited by this paper
• Selective Amplification of the Genome Surrounding Key Placental Genes in Trophoblast Giant Cells.

  2016cited by this paper
• Author response: Structure of the active form of human origin recognition complex and its ATPase motor module

  2016cited by this paper
• Copy Number Variation Is a Fundamental Aspect of the Placental Genome

  2014cited by this paper
• Endocycles: a recurrent evolutionary innovation for post-mitotic cell growth

  2014cited by this paper
• Polyploidization in liver tissue.

  2014cited by this paper
• Aneuploidy, polyploidy and ploidy reversal in the liver.

  2013cited by this paper
• Fundamental differences in endoreplication in mammals and Drosophila revealed by analysis of endocycling and endomitotic cells

  2013cited by this paper
• Endoreplication and polyploidy: insights into development and disease

  2013cited by this paper
• Aneuploidy as a mechanism for stress-induced liver adaptation.

  2012cited by this paper
• Atypical E2F repressors and activators coordinate placental development.

  2012cited by this paper
• Hypertrophy and unconventional cell division of hepatocytes underlie liver regeneration.

  2012cited by this paper
• Canonical and Atypical E2Fs Regulate the Mammalian Endocycle

  2012cited by this paper
• Mutations in ORC1, encoding the largest subunit of the origin recognition complex, cause microcephalic primordial dwarfism resembling Meier-Gorlin syndrome

  2011cited by this paper
• Developmental control of the DNA replication and transcription programs.

  2011cited by this paper
• The ploidy-conveyor of mature hepatocytes as a source of genetic variation

  2010cited by this paper
• Development and function of trophoblast giant cells in the rodent placenta.

  2010cited by this paper
• Endoreplication: polyploidy with purpose.

  2009cited by this paper
• Analysis of mutant origin recognition complex with reduced ATPase activity in vivo and in vitro.

  2008cited by this paper
• The origin recognition complex is dispensable for endoreplication in Drosophila

  2008cited by this paper
• A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice

  2008cited by this paper
• RNA interference targeting ORC1 gene suppresses the proliferation of vascular smooth muscle cells in rats.

  2008cited by this paper
• Regulating the licensing of DNA replication origins in metazoa.

  2006cited by this paper
• Liver-specific pRB loss results in ectopic cell cycle entry and aberrant ploidy.

  2005cited by this paper
• The genes encoding Arabidopsis ORC subunits are E2F targets and the two ORC1 genes are differently expressed in proliferating and endoreplicating cells

  2005cited by this paper
• Novel Splicing Variant of Mouse Orc1 Is Deficient in Nuclear Translocation and Resistant for Proteasome-mediated Degradation*

  2005cited by this paper
• Inducible Cre-mediated control of gene expression in the murine gastrointestinal tract: effect of loss of beta-catenin.

  2004influential reference
• Efficient temporally controlled targeted somatic mutagenesis in hepatocytes of the mouse

  2004influential reference
• Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain.

  2002cited by this paper
• Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain.

  2002cited by this paper
• Functional analysis of mutant and wild-type Drosophila origin recognition complex

  2001cited by this paper
• DNA excision in liver by an albumin‐Cre transgene occurs progressively with age

  2000cited by this paper
• Hepatic polyploidy and liver growth control.

  2000cited by this paper
• High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP

  2000cited by this paper
• The ORC1 homolog orp1 in fission yeast plays a key role in regulating onset of S phase.

  1996cited by this paper
• Cell size, nuclear content, and the development of polyploidy in the Mammalian liver.

  1967cited by this paper
• Short Hospital Stays for Mental Health Care Systematic Review of the Effectiveness of Planned Rapid Responses

  year unknowncited by this paper

• License to Replicate: Mechanisms of Licensing Eukaryotic Origins for DNA Replication

  2025cites this paper
• Molecular Mechanism and Therapeutic Prospects of Origin Recognition Complex 1 (ORC1) in Carcinogenesis

  2025cites this paper
• DNA replication in primary hepatocytes without the six-subunit ORC

  2025cites this paper
• Integrative analysis of DNA replication origins and ORC-/MCM-binding sites in human cells reveals a lack of overlap

  2024cites this paper
• Morpho-histological and Transcriptome Analysis Reveal the Unreduced Sperm Formation Mechanism in cdk1-Depletion Zebrafish

  2024cites this paper
• DNA replication in primary hepatocytes without the six-subunit ORC

  2024influential citation
• Identification and validation of the clinical prediction model and biomarkers based on chromatin regulators in colon cancer by integrated analysis of bulk- and single-cell RNA sequencing data

  2024cites this paper
• Integrative analysis of DNA replication origins and ORC/MCM binding sites in human cells reveals a lack of overlap

  2023cites this paper
• Stemness genes and miR-1247-3p expression associate with clinicopathological parameters and prognosis in lung adenocarcinoma

  2023cites this paper
• Lrwd1 impacts cell proliferation and the silencing of repetitive DNA elements

  2022cites this paper
• Deletion of Orc4 during oogenesis severely reduces polar body extrusion and blocks zygotic DNA replication

  2022cites this paper
• DNA Replication Licensing Factors: Novel Targets for Cancer Therapy via Inhibiting the Stemness of Cancer Cells

  2022cites this paper
• Transcriptomic and Metabolomic Analyses Provide Insights into the Growth and Development Advantages of Triploid Apostichopus japonicus

  2022cites this paper
• Origin recognition complex subunit 1 (ORC1) augments malignant behaviors of lung adenocarcinoma cells via targeting Wnt signaling

  2022cites this paper
• Mammalian Resilience Revealed by a Comparison of Human Diseases and Mouse Models Associated With DNA Helicase Deficiencies

  2022cites this paper
• Developmentally Programmed Switches in DNA Replication: Gene Amplification and Genome-Wide Endoreplication in Tetrahymena

  2022cites this paper
• Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist

  2021cites this paper
• A free-living protist that lacks canonical eukaryotic DNA replication and segregation systems

  2021cites this paper
• Glucagon-like peptide-1 receptor mediates the beneficial effect of liraglutide in an acute lung injury mouse model involving the thioredoxin-interacting protein

  2020cites this paper
• DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1

  2020cites this paper
• A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins

  2020cites this paper
• A Meier-Gorlin Syndrome Mutation in Orc4 Causes Tissue-Specific DNA Replication Defects in Drosophila melanogaster

  2019cites this paper
• Origin recognition complex subunit 1 regulates cell growth and metastasis in glioma by altering activation of ERK and JNK signaling pathway.

  2019cites this paper
• Tissue-Specific DNA Replication Defects in Drosophila melanogaster Caused by a Meier-Gorlin Syndrome Mutation in Orc4

  2019cites this paper
• The broken cycle: E2F dysfunction in cancer

  2019cites this paper