Molecularly imprinted polymer-based catalytic micromotors for selective protein transport.

Jahir Orozco,A. Cortés,Guanzhi Cheng,Sirilak Sattayasamitsathit,W. Gao,Xiaomiao Feng,Yufeng Shen,Joseph Wang

Published 2013 in Journal of the American Chemical Society

ABSTRACT

We demonstrate an attractive nanomachine "capture and transport" target isolation strategy based on molecularly imprinted polymers (MIPs). MIP-based catalytic microtubular engines are prepared by electropolymerization of the outer polymeric layer in the presence of the target analyte (template). Tailor-made selective artificial recognition sites are thus introduced into the tubular microtransporters through complementary nanocavities in the outer polymeric layer. The new microtransporter concept is illustrated using bilayer poly(3,4-ethylenedioxythiophene) (PEDOT)/Pt-Ni microengines and fluorescein isothiocyanate (FITC)-labeled avidin (Av-FITC) as the template. The avidin-imprinted polymeric layer selectively concentrates the fluorescent-tagged protein target onto the moving microengine without the need for additional external functionalization, allowing "on-the-fly" extraction and isolation of Av-FITC from raw serum and saliva samples along with real-time visualization of the protein loading and transport. The new micromachine-MIP-based target isolation strategy can be extended to the capture and transport of other important target molecules, leading toward diverse biomedical and environmental applications.

PUBLICATION RECORD

  • Publication year

    2013

  • Venue

    Journal of the American Chemical Society

  • Publication date

    2013-03-28

  • Fields of study

    Medicine, Materials Science, Chemistry, Engineering

  • Identifiers
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar, PubMed

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