NMR solution structure of the integral membrane enzyme DsbB: functional insights into DsbB-catalyzed disulfide bond formation.

No abstract is available for this paper.

• Publication year

  2008

• Venue

  Molecules and Cells

• Publication date

  2008-09-26

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.molcel.2008.08.028PMID 18922471PMCID PMC2622435
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structure and mechanism of the M2 proton channel of influenza A virus

  2008cited by this paper
• Mutants in DsbB that appear to redirect oxidation through the disulfide isomerization pathway.

  2008cited by this paper
• The role of Dsb proteins of Gram-negative bacteria in the process of pathogenesis.

  2007cited by this paper
• Kinetic Characterization of the Disulfide Bond-forming Enzyme DsbB*

  2007influential reference
• Critical role of a thiolate-quinone charge transfer complex and its adduct form in de novo disulfide bond generation by DsbB.

  2006cited by this paper
• NMR study of the tetrameric KcsA potassium channel in detergent micelles

  2006cited by this paper
• Crystal structure of the DsbB-DsbA complex reveals a mechanism of disulfide bond generation.

  2006influential reference
• Site-directed parallel spin-labeling and paramagnetic relaxation enhancement in structure determination of membrane proteins by solution NMR spectroscopy.

  2006influential reference
• Accessibility of nitroxide side chains: absolute Heisenberg exchange rates from power saturation EPR.

  2005cited by this paper
• The prokaryotic enzyme DsbB may share key structural features with eukaryotic disulfide bond forming oxidoreductases

  2005cited by this paper
• Reactivities of Quinone-free DsbB from Escherichia coli*

  2005cited by this paper
• Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins.

  2004cited by this paper
• DsbB Elicits a Red-shift of Bound Ubiquinone during the Catalysis of DsbA Oxidation*

  2004influential reference
• Charged gels as orienting media for measurement of residual dipolar couplings in soluble and integral membrane proteins.

  2004cited by this paper
• The Xplor-NIH NMR molecular structure determination package.

  2003cited by this paper
• RefDB: A database of uniformly referenced protein chemical shifts

  2003influential reference
• Ile, Leu, and Val methyl assignments of the 723-residue malate synthase G using a new labeling strategy and novel NMR methods.

  2003cited by this paper
• Folding of DsbB in mixed micelles: a kinetic analysis of the stability of a bacterial membrane protein.

  2003cited by this paper
• Hydrogen bonding in high-resolution protein structures: a new method to assess NMR protein geometry.

  2002cited by this paper
• Four cysteines of the membrane protein DsbB act in concert to oxidize its substrate DsbA

  2002influential reference
• Hydrogen bonds in human ubiquitin reflected in temperature coefficients of amide protons.

  2002cited by this paper
• Structure, dynamics and electrostatics of the active site of glutaredoxin 3 from Escherichia coli: comparison with functionally related proteins.

  2001cited by this paper
• A novel NMR method for determining the interfaces of large protein–protein complexes

  2000cited by this paper
• Roles of a conserved arginine residue of DsbB in linking protein disulfide-bond-formation pathway to the respiratory chain of Escherichia coli.

  2000cited by this paper
• 13C NMR chemical shifts can predict disulfide bond formation

  2000cited by this paper
• Protein dynamics measurements by TROSY-based NMR experiments.

  2000cited by this paper
• Disulfide Bonds Are Generated by Quinone Reduction*

  2000cited by this paper
• Oxidative protein folding is driven by the electron transport system.

  1999cited by this paper
• Structural changes linked to proton translocation by subunit c of the ATP synthase

  1999cited by this paper
• Protein backbone angle restraints from searching a database for chemical shift and sequence homology

  1999cited by this paper
• MOLMOL: a program for display and analysis of macromolecular structures.

  1996cited by this paper
• Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: a site-directed spin-labeling study.

  1996cited by this paper
• Reactivity and ionization of the active site cysteine residues of DsbA, a protein required for disulfide bond formation in vivo.

  1994cited by this paper
• A collision gradient method to determine the immersion depth of nitroxides in lipid bilayers: application to spin-labeled mutants of bacteriorhodopsin.

  1994cited by this paper
• Crystal structure of the DsbA protein required for disulphide bond formation in vivo

  1993cited by this paper
• The reactive and destabilizing disulfide bond of DsbA, a protein required for protein disulfide bond formation in vivo.

  1993cited by this paper
• Identification of a protein required for disulfide bond formation in vivo.

  1991cited by this paper
• Identification and characterization of the functional amino acids at the active center of pig liver thioltransferase by site-directed mutagenesis.

  1991cited by this paper
• Use of a site-directed triple mutant to trap intermediates: demonstration that the flavin C(4a)-thiol adduct and reduced flavin are kinetically competent intermediates in mercuric ion reductase.

  1990cited by this paper
• Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology

  1987cited by this paper
• Proton magnetic resonance spectroscopic studies of the interaction of ubiquinone-10 with phospholipid model membranes.

  1986cited by this paper
• Lipid bilayer thickness varies linearly with acyl chain length in fluid phosphatidylcholine vesicles.

  1983cited by this paper
• Differential reactivity of the functional sulfhydryl groups of cysteine-32 and cysteine-35 present in the reduced form of thioredoxin from Escherichia coli.

  1980cited by this paper
• Role of quinones in electron transport to oxygen and nitrate in Escherichia coli. Studies with a ubiA- menA- double quinone mutant.

  1977cited by this paper
• The kinetics of formation of native ribonuclease during oxidation of the reduced polypeptide chain.

  1961cited by this paper

• Drug-resistant Acinetobacter baumannii: mortality, emerging treatments, and future pharmacological targets for a WHO priority pathogen

  2025cites this paper
• MEMO-Stab2: Multi-View Sequence-Based Deep Learning Framework for Predicting Mutation-Induced Stability Changes in Transmembrane Proteins

  2025cites this paper
• Non-canonical amino acids for site-directed spin labeling of membrane proteins.

  2024cites this paper
• MEMO-stab: Sequence-based Annotation of Mutation Effect on Transmembrane Protein Stability with Protein Language Model-Driven Machine Learning

  2024cites this paper
• Expression of Cell-Adhesion Molecules in E. coli: A High Throughput Screening to Identify Paracellular Modulators

  2023cites this paper
• Dark nanodiscs for evaluating membrane protein thermostability by differential scanning fluorimetry.

  2023cites this paper
• Solution NMR investigations of integral membrane proteins: Challenges and innovations.

  2023cites this paper
• Sparse pseudocontact shift NMR data obtained from a non-canonical amino acid-linked lanthanide tag improves integral membrane protein structure prediction

  2023cites this paper
• Distinct enzymatic strategies for de novo generation of disulfide bonds in membranes

  2023cites this paper
• Dark nanodiscs as a model membrane for evaluating membrane protein thermostability by differential scanning fluorimetry

  2023cites this paper
• Paramagnetic Chemical Probes for Studying Biological Macromolecules

  2022cites this paper
• Pseudocontact shift NMR data obtained from a non-canonical amino acid-linked lanthanide tag improves integral membrane protein structure prediction

  2022influential citation
• Generowanie MostkÓW Disiarczkowych W BiaŁKach – RÓŻNorodnoŚĆ Strukturalna I Funkcjonalna BiaŁEk Dsba

  2021cites this paper
• Protein Labeling and Structure Determination by NMR Spectroscopy

  2021cites this paper
• Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis

  2021cites this paper
• Structural basis of SARS-CoV-2 spike protein induced by ACE2

  2020cites this paper
• Spotlight on the Ballet of Proteins: The Structural Dynamic Properties of Proteins Illuminated by Solution NMR

  2020cites this paper
• Protein Disulfide Exchange by the Intramembrane Enzymes DsbB, DsbD, and CcdA.

  2020cites this paper
• Other Specialized Flow NMR

  2020cites this paper
• Fake It ‘Till You Make It—The Pursuit of Suitable Membrane Mimetics for Membrane Protein Biophysics

  2020cites this paper
• Termini restraining of small membrane proteins enables structure determination at near-atomic resolution

  2020cites this paper
• Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR

  2020cites this paper
• Solution NMR spectroscopy of membrane proteins.

  2020cites this paper
• Oxidoreductase disulfide bond proteins DsbA and DsbB form an active redox pair in Chlamydia trachomatis, a bacterium with disulfide dependent infection and development

  2019influential citation
• Engineering of the Dsb (disulfide bond) proteins – contribution towards understanding their mechanism of action and their applications in biotechnology and medicine

  2019cites this paper
• Entropy-Driven Mechanisms between Disulfide-Bond Formation Protein A (DsbA) and B (DsbB) in Escherichia coli

  2019cites this paper
• Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in a Lipid Environment.

  2019cites this paper
• How Detergent Impacts Membrane Proteins: Atomic-Level Views of Mitochondrial Carriers in Dodecylphosphocholine

  2018cites this paper
• Engineered Protein Model of the ATP synthase H+- Channel Shows No Salt Bridge at the Rotor-Stator Interface

  2018cites this paper
• Membrane properties that shape the evolution of membrane enzymes.

  2018cites this paper
• Effects of chelator lipids, paramagnetic metal ions and trehalose on liposomes by solid-state NMR.

  2018cites this paper
• Solution Structure and Elevator Mechanism of the Membrane Electron Transporter CcdA

  2018cites this paper
• Enzyme targets for drug design of new anti-virulence therapeutics.

  2018cites this paper
• Membrane mimetics for solution NMR studies of membrane proteins

  2017cites this paper
• Bacterial thiol oxidoreductases — from basic research to new antibacterial strategies

  2017cites this paper
• Application of Solution NMR to Structural Studies on α-Helical Integral Membrane Proteins

  2017cites this paper
• Systematic evaluation of CS‐Rosetta for membrane protein structure prediction with sparse NOE restraints

  2017influential citation
• Comparison of NMR and crystal structures of membrane proteins and computational refinement to improve model quality

  2017cites this paper
• Structure formation during translocon-unassisted co-translational membrane protein folding

  2017cites this paper
• Applications of NMR to membrane proteins.

  2017influential citation
• Chemistry and Enzymology of Disulfide Cross-Linking in Proteins.

  2017cites this paper
• Intramembrane Thiol Oxidoreductases: Evolutionary Convergence and Structural Controversy.

  2017cites this paper
• The function of a membrane protein : studies of structure and dynamics by NMR

  2017cites this paper
• Documentation of an Imperative To Improve Methods for Predicting Membrane Protein Stability

  2016cites this paper
• The membrane topology of vitamin K epoxide reductase is conserved between human isoforms and the bacterial enzyme.

  2016cites this paper
• NMR as a tool to investigate the structure, dynamics and function of membrane proteins

  2016cites this paper
• NMR of Membrane Proteins: Beyond Crystals.

  2016cites this paper
• Nuclear magnetic resonance (NMR) applied to membrane–protein complexes

  2016cites this paper
• Solution Nuclear Magnetic Resonance Spectroscopy of Integral Membrane Proteins

  2016cites this paper
• The use of nuclear magnetic resonance in study of structurally dynamic membrane proteins

  2016cites this paper
• A functional NMR for membrane proteins: dynamics, ligand binding, and allosteric modulation

  2016cites this paper
• NMR studies of membrane proteins

  2015cites this paper
• Membrane proteins in their native habitat as seen by solid‐state NMR spectroscopy

  2015cites this paper
• Cellular Disulfide Bond Formation in Bioactive Peptides and Proteins

  2015cites this paper
• The role of YidC in the assembly of Rat VKORC1 in the inner membrane of Escherichia coli

  2015cites this paper
• Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation

  2015cites this paper
• Deuterated detergents for structural and functional studies of membrane proteins: Properties, chemical synthesis and applications

  2015cites this paper
• Phylogeny of the Vitamin K 2,3-Epoxide Reductase (VKOR) Family and Evolutionary Relationship to the Disulfide Bond Formation Protein B (DsbB) Family

  2015cites this paper
• Solution NMR Structure Determination of Polytopic α-Helical Membrane Proteins: A Guide to Spin Label Paramagnetic Relaxation Enhancement Restraints.

  2015cites this paper
• Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation

  2015cites this paper
• NMR structures of membrane proteins in phospholipid bilayers

  2014cites this paper
• NMR Studies of Membrane Associating Peptides and Implications in Autotransporter Function

  2014cites this paper
• Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints

  2014cites this paper
• Differential Dynamics of Extracellular and Cytoplasmic Domains in Denatured States of Rhodopsin

  2014cites this paper
• Use of a Paramagnetic Spin Label for Determination of Long-Range Distance Constraints in Solid-State NMR

  2014cites this paper
• Recent advances in magic angle spinning solid state NMR of membrane proteins.

  2014cites this paper
• Synthesis of uniformly deuterated n-dodecyl-β-D-maltoside (d39-DDM) for solubilization of membrane proteins in TROSY NMR experiments.

  2014cites this paper
• Folding energetics and oligomerization of polytopic α-helical transmembrane proteins.

  2014cites this paper
• Mapping membrane protein backbone dynamics: a comparison of site-directed spin labeling with NMR 15N-relaxation measurements.

  2014cites this paper
• Structures of an intramembrane vitamin K epoxide reductase homolog reveal control mechanisms for electron transfer

  2014cites this paper
• Structure determination of α-helical membrane proteins by solution-state NMR: emphasis on retinal proteins.

  2014influential citation
• Intermolecular detergent-membrane protein noes for the characterization of the dynamics of membrane protein-detergent complexes.

  2014cites this paper
• Disarming Burkholderia pseudomallei: Structural and Functional Characterization of a Disulfide Oxidoreductase (DsbA) Required for Virulence In Vivo

  2014influential citation
• Membrane protein structures for rational antimicrobial drug design

  2014cites this paper
• Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR

  2013cites this paper
• Combination of 15N reverse labeling and afterglow spectroscopy for assigning membrane protein spectra by magic-angle-spinning solid-state NMR: application to the multidrug resistance protein EmrE

  2013cites this paper
• Structures of the excited states of phospholamban and shifts in their populations upon phosphorylation.

  2013cites this paper
• The present and future of solution NMR in investigating the structure and dynamics of channels and transporters

  2013cites this paper
• A review on the role of quinones in renal disorders

  2013influential citation
• DNA-Nanotube-Enabled NMR Structure Determination of Membrane Proteins

  2013cites this paper
• Thioredoxin-like proteins in F and other plasmid systems.

  2013cites this paper
• Structure of the disulfide bond generating membrane protein DsbB in the lipid bilayer.

  2013cites this paper
• Structural Basis of Disulfide Bond Formation in the Bacterial Periplasm and Mammalian ER

  2013cites this paper
• Combination of 15N reverse labeling and afterglow spectroscopy for assigning membrane protein spectra by magic-angle-spinning solid-state NMR: application to the multidrug resistance protein EmrE

  2013influential citation
• Perspectives of solution NMR spectroscopy for structural and functional studies of integral membrane proteins

  2013cites this paper
• Real-time Monitoring of Intermediates Reveals the Reaction Pathway in the Thiol-Disulfide Exchange between Disulfide Bond Formation Protein A (DsbA) and B (DsbB) on a Membrane-immobilized Quartz Crystal Microbalance (QCM) System*

  2013cites this paper
• Ero1-α and PDIs constitute a hierarchical electron transfer network of endoplasmic reticulum oxidoreductases

  2013cites this paper
• Biomolecular magic-angle spinning solid-state NMR: recent methods and applications.

  2013cites this paper
• Detergent-mediated protein aggregation.

  2013cites this paper
• Solid-state NMR study of a 41 kDa membrane protein complex DsbA/DsbB.

  2013cites this paper
• Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein

  2013cites this paper
• DNA nanotubes for NMR structure determination of membrane proteins

  2013cites this paper
• Characterization of the Human Sigma-1 Receptor Chaperone Domain Structure and Binding Immunoglobulin Protein (BiP) Interactions*

  2013cites this paper
• The quiet renaissance of protein nuclear magnetic resonance.

  2013cites this paper
• Biochemical and biophysical studies of the prokaryotic proton dependent oligopeptide transporters

  2013cites this paper
• Helical membrane protein conformations and their environment

  2013cites this paper
• Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR

  2013cites this paper
• Solution NMR spectroscopy for the determination of structures of membrane proteins in a lipid environment.

  2013cites this paper
• Advances in NMR structures of integral membrane proteins.

  2013influential citation
• Real-time Monitoring of Intermediates Reveals the Reaction Pathway in the Thiol-Disulfide Exchange between Disulfide Bond Formation Protein A ( DsbA ) and B ( DsbB ) on a Membrane-immobilized Quartz Crystal Microbalance ( QCM )

  2013cites this paper