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Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

L. Jayakody,Jing-Jing Liu,E. Yun,T. Turner,E. Oh,Yong‐Su Jin

Published 2018 in Biotechnology and Bioengineering

ABSTRACT

Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a biodegradable surfactant, ethyl‐β‐d‐glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT‐1) and intracellular β‐glucosidase (GH1‐1) originating from Neurospora crassa. We identified the formation of ethyl‐β‐d‐glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1‐1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl‐β‐d‐glucoside/g cellulose at a rate of 0.30 ± 0.02 g·L−1·h−1 and 0.09 ± 01 g·L−1·h−1, respectively, during the SSF of Avicel PH‐101 cellulose, supplemented only with Celluclast 1.5 L. Herein, we report a possible coproduction of a value‐added chemical (alkyl‐glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1‐1 in engineered S. cerevisiae. This coproduction could have a substantial effect on the overall technoeconomic feasibility of theSSF of cellulose.

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