In this study, a calibration model based on Near-infrared spectroscopy (NIR) technique and chemometrics method was developed for rapid and non-destructive detecting the polysaccharide contents of lentinula edodes samples collected from different regions. The polysaccharide contents of these samples were firstly determined by standard phenol-sulphruic acid method. Then, NIR spectra of these samples were collected by using Fourier transform infrared spectrometry. Based on these experimental data, a random forest method was further used to distinguish the regions of these samples, with a classification accuracy of 96.6%. After that, a rapid, accurate, and quantitative model was established for predicting the polysaccharide contents of these samples. In the model establishing process, some signal pre-treatment methods were optimized, and the validation results with highest determination coefficient (R2) and low root mean square errors of prediction (RMSEP) were, 0.925 and 0.720, respectively. These results showed that combined NIR technique with chemometrics was an effective and green method for lentinula edodes quality control.
Application of near infrared spectroscopy for rapid determination the geographical regions and polysaccharides contents of Lentinula edodes.
Yi Xie,R. Zhou,Hua-lin Xie,Yi Yu,Shuihan Zhang,Chen-Xi Zhao,Jianhua Huang,Luqi Huang
Published 2019 in International Journal of Biological Macromolecules
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- Publication year
2019
- Venue
International Journal of Biological Macromolecules
- Publication date
2019-02-01
- Fields of study
Medicine, Materials Science, Chemistry, Environmental Science
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- External record
- Source metadata
Semantic Scholar, PubMed
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