Crystallographic and X-ray absorption spectroscopic characterization of Helicobacter pylori UreE bound to Ni²⁺ and Zn²⁺ reveals a role for the disordered C-terminal arm in metal trafficking.

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Biochemical Journal

• Publication date

  2012-02-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1042/BJ20111659PMID 22010876PMCID PMC3501991
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Chemistry of Ni2+ in urease: sensing, trafficking, and catalysis.

  2011cited by this paper
• Crystal structures of apo and metal-bound forms of the UreE protein from Helicobacter pylori: role of multiple metal binding sites.

  2010cited by this paper
• Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure.

  2010cited by this paper
• Mutagenesis of Klebsiella aerogenes UreG to probe nickel binding and interactions with other urease-related proteins.

  2010cited by this paper
• Interplay of metal ions and urease.

  2009cited by this paper
• Helicobacter pylori UreE, a urease accessory protein: specific Ni(2+)- and Zn(2+)-binding properties and interaction with its cognate UreG.

  2009cited by this paper
• Chaperones of Nickel Metabolism

  2007cited by this paper
• Phaser crystallographic software

  2007cited by this paper
• The nickel site of Bacillus pasteurii UreE, a urease metallo-chaperone, as revealed by metal-binding studies and X-ray absorption spectroscopy.

  2006cited by this paper
• The burden borne by urease.

  2005cited by this paper
• Auto-rickshaw: an automated crystal structure determination platform as an efficient tool for the validation of an X-ray diffraction experiment.

  2005cited by this paper
• ATHENA, ARTEMIS, HEPHAESTUS: data analysis for X-ray absorption spectroscopy using IFEFFIT.

  2005cited by this paper
• SIXpack: a graphical user interface for XAS analysis using IFEFFIT

  2005cited by this paper
• Breaking good resolutions with ARP/wARP.

  2004cited by this paper
• Nickel trafficking: insights into the fold and function of UreE, a urease metallochaperone.

  2004cited by this paper
• Dependence of Helicobacter pylori Urease Activity on the Nickel-Sequestering Ability of the UreE Accessory Protein

  2003cited by this paper
• Unraveling the substrate-metal binding site of ferrochelatase: an X-ray absorption spectroscopic study.

  2002cited by this paper
• Macromolecular phasing with SHELXE

  2002cited by this paper
• Substructure solution with SHELXD.

  2002cited by this paper
• EXAFS analysis using FEFF and FEFFIT.

  2001cited by this paper
• Structural Basis for Ni2+Transport and Assembly of the Urease Active Site by the Metallochaperone UreE from Bacillus pasteurii *

  2001cited by this paper
• Supramolecular assembly and acid resistance of Helicobacter pylori urease

  2001cited by this paper
• Crystal Structure of Klebsiella aerogenesUreE, a Nickel-binding Metallochaperone for Urease Activation*

  2001cited by this paper
• UreE stimulation of GTP-dependent urease activation in the UreD-UreF-UreG-urease apoprotein complex.

  2000cited by this paper
• Automated protein model building combined with iterative structure refinement

  1999cited by this paper
• A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels.

  1999cited by this paper
• X-ray absorption spectroscopy of a new zinc site in the fur protein from Escherichia coli.

  1998cited by this paper
• [20] Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• [27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods.

  1997cited by this paper
• Refinement of macromolecular structures by the maximum-likelihood method.

  1997cited by this paper
• Metal ion interaction with urease and UreD-urease apoproteins.

  1996cited by this paper
• The crystal structure of urease from Klebsiella aerogenes.

  1995cited by this paper
• NMRPipe: A multidimensional spectral processing system based on UNIX pipes

  1995cited by this paper
• Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation

  1995cited by this paper
• Multiple-scattering calculations of x-ray-absorption spectra.

  1995cited by this paper
• Requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter

  1995cited by this paper
• Copyright � 1995, American Society for Microbiology Molecular Biology of Microbial Ureases

  1995cited by this paper
• The CCP4 suite: programs for protein crystallography.

  1994cited by this paper
• Purification and characterization of Klebsiella aerogenes UreE protein: A nickel‐binding protein that functions in urease metallocenter assembly

  1993cited by this paper
• Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis

  1992cited by this paper
• X-ray spectroscopic studies of nickel complexes, with application to the structure of nickel sites in hydrogenases

  1991cited by this paper
• Copper K-extended x-ray absorption fine structure studies of oxidized and reduced dopamine beta-hydroxylase. Confirmation of a sulfur ligand to copper(I) in the reduced enzyme.

  1991cited by this paper
• Accurate Bond and Angle Parameters for X-ray Protein Structure Refinement

  1991cited by this paper
• Microbial ureases: significance, regulation, and molecular characterization.

  1989cited by this paper
• X-ray Absorption Spectroscopy of Metal-Histidine Coordination in Metalloproteins. Exact Simulation of the EXAFS of Tetrakis(imidazole)copper(II) Nitrate and Other Copper-Imidazole Complexes by the Use of a Multiple-Scattering Treatment

  1987cited by this paper
• Electronic Reprint Biological Crystallography Maximum-likelihood Density Modification Using Pattern Recognition of Structural Motifs Pattern Recognition of Structural Motifs

  year unknowncited by this paper

• Structural insights into how metallochaperones UreE and UreG interact to deliver a toxic metal to urease

  2025cites this paper
• A comprehensive review on microbial urease: features and industrial applications

  2025cites this paper
• Sumner’s legacy: a century of urease crystals and recent structural advances

  2025cites this paper
• One His, two His…the emerging roles of histidine in cellular nickel trafficking.

  2024cites this paper
• The structure of the high-affinity nickel-binding site in the Ni,Zn-HypA•UreE2 complex.

  2023cites this paper
• Functional contacts for activation of urease from Helicobacter pylori: an integrated approach using evolutionary couplings, in-cell enzymatic assays, and computational docking

  2023cites this paper
• Delivering a toxic metal to the active site of urease

  2023cites this paper
• Urease of Aspergillus fumigatus Is Required for Survival in Macrophages and Virulence

  2023cites this paper
• Nickel as a Virulence Factor in the Class I Bacterial Carcinogen, Helicobacter pylori.

  2021cites this paper
• Substitution of residues in UreG to investigate UreE interactions and nickel binding in a predominant urease gene cluster from the ruminal metagenome.

  2020cites this paper
• Intrinsic disorder in the nickel-dependent urease network.

  2020cites this paper
• Ni Site Structure and Function in Biological Sensing and Enzyme Activity

  2019cites this paper
• Ni(II) Sensing by RcnR Does Not Require an FrmR-Like Intersubunit Linkage.

  2019cites this paper
• The carbon monoxide dehydrogenase accessory protein CooJ is a histidine-rich multidomain dimer containing an unexpected Ni(II)-binding site

  2019cites this paper
• The metal-binding properties of the long chaplin from Streptomyces mobaraensis: A bioinformatic and biochemical approach.

  2019cites this paper
• Nickel Metalloregulators and Chaperones

  2019influential citation
• The Maturation Pathway of Nickel Urease

  2019influential citation
• The Role of the Metallochaperone HypA in the Acid Survival and Activities of Nickel Enzymes in Helicobacter pylori

  2018cites this paper
• Acid-Triggered O-O Bond Heterolysis of a Nonheme FeIII (OOH) Species for the Stereospecific Hydroxylation of Strong C-H Bonds.

  2018cites this paper
• The Helicobacter pylori HypA·UreE2 Complex Contains a Novel High-Affinity Ni(II)-Binding Site.

  2018cites this paper
• Chloride Supports O2 Activation in the D201G Facial Triad Variant of Factor-Inhibiting Hypoxia Inducible Factor, an α-Ketoglutarate Dependent Oxygenase.

  2018cites this paper
• Structure and dynamics of Helicobacter pylori nickel-chaperone HypA: an integrated approach using NMR spectroscopy, functional assays and computational tools

  2018cites this paper
• Metabolic functions of the human gut microbiota: the role of metalloenzymes

  2018cites this paper
• Role of the Facial Triad in Factor Inhibiting HIF (FIH): Ligand Binding, Substrate Selectivity, and Coupling

  2018cites this paper
• PROTEIN STRUCTURE REPORT Crystal structure of the UBR-box from UBR6/FBXO11 reveals domain swapping mediated by zinc binding

  2017cites this paper
• Relationship Between Structure and Function in Nickel Proteins and Enzymes

  2017cites this paper
• Glutamate Ligation in the Ni(II)- and Co(II)-Responsive Escherichia coli Transcriptional Regulator, RcnR.

  2017cites this paper
• An XAS investigation of the nickel site structure in the transcriptional regulator InrS.

  2017cites this paper
• Nickel Ligation of the N-Terminal Amine of HypA Is Required for Urease Maturation in Helicobacter pylori.

  2017cites this paper
• Exploration into the nickel ‘microcosmos’ in prokaryotes

  2016cites this paper
• Non-thiolate ligation of nickel by nucleotide-free UreG of Klebsiella aerogenes

  2016cites this paper
• Rapid Hydrogen and Oxygen Atom Transfer by a High-Valent Nickel-Oxygen Species.

  2016cites this paper
• Differential effects of zinc binding on structured and disordered regions in the multidomain STIL protein

  2016cites this paper
• Evolution of Macromolecular Docking Techniques: The Case Study of Nickel and Iron Metabolism in Pathogenic Bacteria

  2015cites this paper
• Intrinsic disorder and metal binding in UreG proteins from Archae hyperthermophiles: GTPase enzymes involved in the activation of Ni(II) dependent urease

  2015cites this paper
• UreE-UreG Complex Facilitates Nickel Transfer and Preactivates GTPase of UreG in Helicobacter pylori*♦

  2015influential citation
• Reactivity of a Nickel(II) Bis(amidate) Complex with meta-Chloroperbenzoic Acid: Formation of a Potent Oxidizing Species.

  2015cites this paper
• Structural and biochemical studies of Sporosarcina pasteurii UreE: a nickel-chaperone involved in the urease activation process

  2014influential citation
• Nickel translocation between metallochaperones HypA and UreE in Helicobacter pylori.

  2014cites this paper
• Ca K-Edge XAS as a Probe of Calcium Centers in Complex Systems

  2014cites this paper
• Unique Effect of Cu(II) in the Metal-Induced Amyloid Formation of β-2-Microglobulin

  2014cites this paper
• Ni²⁺ chemistry in pathogens--a possible target for eradication.

  2014cites this paper
• Molecular landscape of the interaction between the urease accessory proteins UreE and UreG.

  2014cites this paper
• Nickel binding properties of Helicobacter pylori UreF, an accessory protein in the nickel-based activation of urease

  2014cites this paper
• Nickel metallomics: general themes guiding nickel homeostasis.

  2013cites this paper
• Selectivity of Ni(II) and Zn(II) binding to Sporosarcina pasteurii UreE, a metallochaperone in the urease assembly: a calorimetric and crystallographic study

  2013cites this paper
• Structure of the UreD–UreF–UreG–UreE complex in Helicobacter pylori: a model study

  2013cites this paper
• Nickel and human health.

  2013cites this paper
• Nickel trafficking system responsible for urease maturation in Helicobacter pylori.

  2013cites this paper
• Biosynthesis of the Urease Metallocenter*

  2013cites this paper
• Structure of UreG/UreF/UreH Complex Reveals How Urease Accessory Proteins Facilitate Maturation of Helicobacter pylori Urease

  2013cites this paper
• Helicobacter pylori hydrogenase accessory protein HypA and urease accessory protein UreG compete with each other for UreE recognition.

  2012cites this paper
• Etude des réseaux d'interactions protéiques impliqués dans le trafic du nickel et de l'ammoniac et de leurs rôles dans la virulence chez Helicobacter pylori

  2012cites this paper
• Specific metal recognition in nickel trafficking.

  2012cites this paper