Characterization of MicroRNA Expression Profiles and the Discovery of Novel MicroRNAs Involved in Cancer during Human Embryonic Development

MicroRNAs (miRNAs), approximately 22-nucleotide non-coding RNA molecules, regulate a variety of pivotal physiological or pathological processes, including embryonic development and tumorigenesis. To obtain comprehensive expression profiles of miRNAs in human embryos, we characterized miRNA expression in weeks 4-6 of human embryonic development using miRNA microarrays and identified 50 human-embryo-specific miRNAs (HES-miRNAs). Furthermore, we selected three non-conserved or primate-specific miRNAs, hsa-miR-638, -720, and -1280, and examined their expression levels in various normal and tumor tissues. The results show that expression of most miRNAs is extremely low during early human embryonic development. In addition, the expression of some non-conserved or primate-specific miRNAs is significantly different between tumor and the corresponding normal tissue samples, suggesting that the miRNAs are closely related to the pathological processes of various tumors. This study presents the first comprehensive overview of miRNA expression during human embryonic development and offers immediate evidence of the relationship between human early embryonic development and tumorigenesis.

• Publication year

  2013

• Venue

  PLoS ONE

• Publication date

  2013-08-02

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1371/journal.pone.0069230PMID 23936320PMCID 3732277
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Expression of hsa-miR-638, hsa-miR-720, and hsa-miR-1280 differs significantly between tumor and corresponding normal tissues.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• Most miRNAs show extremely low expression during early human embryonic development.

  Confidence 0.93

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• Fifty human-embryo-specific miRNAs were identified from the embryonic expression profiles.

  Confidence 0.96

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• MiRNA microarrays were used to profile miRNA expression in weeks 4-6 of human embryonic development.

  Confidence 0.98

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review

• hsa-mir-1280

  miRNA

  A human miRNA examined for expression differences across normal and tumor tissues.

  Aliases: miR-1280

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• hsa-mir-638

  miRNA

  A human miRNA examined for expression differences across normal and tumor tissues.

  Aliases: miR-638

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• hsa-mir-720

  miRNA

  A human miRNA examined for expression differences across normal and tumor tissues.

  Aliases: miR-720

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• human-embryo-specific mirnas

  molecule set

  A set of miRNAs identified as specific to human embryo samples in this expression survey.

  Aliases: HES-miRNAs

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• mirna microarrays

  method

  A microarray-based assay used here to measure miRNA expression across embryonic samples.

  Aliases: microRNA microarrays

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• normal and tumor tissues

  sample type

  Matched healthy and cancerous tissue samples used to compare miRNA expression levels.

  Aliases: normal tissue samples and tumor tissue samples

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• tumorigenesis

  pathological process

  The cancer-related pathological process discussed in relation to altered miRNA expression.

  Aliases: cancer development

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review
• weeks 4-6 of human embryonic development

  developmental stage

  The early embryonic stage examined for miRNA expression profiling in human samples.

  Aliases: human embryonic weeks 4-6, early human embryonic development

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewKiller Whale (322360f1c1) reviewB (s683577b42) review

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