Immune Activation of RAW264.7 Macrophages by Low Molecular Weight Fucoidan Extracted from New Zealand Undaria pinnatifida.

Fucoidan, a sulfated polysaccharide extracted from brown seaweeds, has been shown to possess various bioactivities. In particular, low molecular weight fucoidan (LMWF) has been shown to have better bioactivities. In this study, a LMWF (<10 kDa) was extracted from New Zealand Undaria pinnatifida and investigated for its immune modulation effects. LMWF at a concentration range from 1 to 50 μg/mL exerted an effective immune activation in RAW264.7 macrophages. LMWF treatment promoted significant NO release, iNOS expression, and TNF-α and IL-6 secretion in a concentration-dependent manner. It also significantly stimulated the activation of NF-κB and MAPK signaling pathways, and specific inhibitors of NF-κB and MAPK pathways diminished the stimulation, confirming the activation pathways. These results indicate that LMWF possesses potential health benefits through immune-stimulation, which may lead to future pharmaceutical development.

• Publication year

  2018

• Venue

  Journal of Agricultural and Food Chemistry

• Publication date

  2018-09-27

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1021/acs.jafc.8b03698PMID 30257559
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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