Drought stress is the most important abiotic stress that constrains crop production and reduces yield drastically. The germplasm of most of the cultivated crops possesses numerous unknown drought stress tolerant genes. Moreover, there are many reports suggesting that the wild species of most of the modern cultivars have abiotic stress tolerant genes. Due to climate change and population booms, food security has become a global issue. To develop drought tolerant crop varieties knowledge of various genes involved in drought stress is required. Different reverse genetic approaches such as virus-induced gene silencing (VIGS), clustered regularly interspace short palindromic repeat (CRISPR), targeting induced local lesions in genomes (TILLING) and expressed sequence tags (ESTs) have been used extensively to study the functionality of different genes involved in response to drought stress. In this review, we described the contributions of different techniques of functional genomics in the study of drought tolerant genes.
Milestones achieved in response to drought stress through reverse genetic approaches
Baljeet Singh,S. Kukreja,Umesh Goutam
Published 2018 in F1000Research
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- Publication year
2018
- Venue
F1000Research
- Publication date
2018-08-17
- Fields of study
Agricultural and Food Sciences, Medicine, Biology, Environmental Science
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- External record
- Source metadata
Semantic Scholar, PubMed
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