Structural characterization, hypoglycemic effects and mechanism of a novel polysaccharide from Tetrastigma hemsleyanum Diels et Gilg.

In the present study, a novel polysaccharide (THDP-3) purified from cane leaves of Tetrastigma hemsleyanum Diels et Gilg was obtained via anion exchange chromatography and the structural analyses of the compound indicated that THDP-3 is a homogenous polysaccharide with molecular weight of 77.98 kDa. Our results show that it consists of rhamnose, arabinose, mannose, glucose and galactose at molar ratio of 1.0: 1.3: 2.5: 2.3:3.1 with main backbones of →4)-α-D-GalAp-(1→, →4)-β-D-Galp-(1→ and →4)-α-D-Glcp-(1→, and main branches of β-D-Manp-(1→, →3,6-β-D-Manp-1→ and α-D-Araf-(1→. In the anti-diabetic assays, THDP-3 showed significant hypoglycemic activity in alloxan-induced diabetic mice. In addition, THDP-3 was found regulating the expression of glucokinase, amp-activated protein kinase, glucose-6-phosphatase and phosphoenolpyruvate carboxykinase. These new findings indicate the high potential in therapeutic mechanisms investigation using THDP-3. Moreover, our results of hypoglycemic activity using THDP-3 demonstrated that the compound could serve as a potential anti-diabetic agent.

• Publication year

  2019

• Venue

  International Journal of Biological Macromolecules

• Publication date

  2019-02-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.ijbiomac.2018.11.085PMID 30447363
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Hypoglycemic Effects of a Polysaccharide from Tetrastigma hemsleyanum Diels & Gilg in Alloxan‐Induced Diabetic Mice

  2018cited by this paper
• Isolation, purification, structural characterization and immunostimulatory activity of water-soluble polysaccharides from Lepidium meyenii.

  2018cited by this paper
• Structural characterization and immunomodulatory activity of a new polysaccharide from jellyfish.

  2017cited by this paper
• Preliminary structural characterization and hypoglycemic effects of an acidic polysaccharide SERP1 from the residue of Sarcandra glabra.

  2017cited by this paper
• Physicochemical properties and antidiabetic effects of a polysaccharide from corn silk in high-fat diet and streptozotocin-induced diabetic mice.

  2017cited by this paper
• Structural characterization and antioxidant activity in vitro of polysaccharides from angelica and astragalus.

  2016cited by this paper
• Rosmarinic acid ameliorates hyperglycemia and insulin sensitivity in diabetic rats, potentially by modulating the expression of PEPCK and GLUT4

  2016cited by this paper
• Mechanisms underlying the effect of polysaccharides in the treatment of type 2 diabetes: A review.

  2016cited by this paper
• Structural elucidation and protective role of a polysaccharide from Sargassum fusiforme on ameliorating learning and memory deficiencies in mice.

  2016cited by this paper
• Characterization and hypoglycemic activity of a β-pyran polysaccharides from bamboo shoot (Leleba oldhami Nakal) shells.

  2016cited by this paper
• Structural characterization and immunomodulatory activity of Grifola frondosa polysaccharide via toll-like receptor 4-mitogen-activated protein kinases-nuclear factor κB pathways.

  2016cited by this paper
• Purification, structural characterization and in vitro antioxidant activity of a novel polysaccharide from Boshuzhi.

  2016cited by this paper
• Treatment and Diagnosis of Diabetes Mellitus and Its Complication: Advanced Approaches.

  2015cited by this paper
• Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.

  2015cited by this paper
• AMPK and autophagy in glucose/glycogen metabolism.

  2015cited by this paper
• Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp.

  2015cited by this paper
• Structural characterization and antioxidant activity of a novel heteropolysaccharide from the submerged fermentation mycelia of Ganoderma capense.

  2015cited by this paper
• Structural, thermal, and anti-inflammatory properties of a novel pectic polysaccharide from alfalfa (Medicago sativa L.) stem.

  2015cited by this paper
• Structural characterization and biological activities of a novel polysaccharide from cultured Cordyceps militaris and its sulfated derivative.

  2015cited by this paper
• Extraction of Flavonoids from Tetrastigma hemsleyanum Diels et Gilg and Their Antioxidant Activity

  2015cited by this paper
• Inhibition of α-glucosidase by polysaccharides from the fruit hull of Camellia oleifera Abel.

  2015cited by this paper
• Effects of superfine grinding on physicochemical and antioxidant properties of Lycium barbarum polysaccharides

  2014cited by this paper
• Structural studies of the pectic polysaccharide from Siberian fir (Abies sibirica Ledeb.).

  2013cited by this paper
• Characterization and cytotoxic activity of sulfated derivatives of polysaccharides from Agaricus brasiliensis.

  2013cited by this paper
• Isolation, purification, and structural characterization of a novel polysaccharide from Ganoderma capense.

  2013cited by this paper
• Structure and antioxidant activity of polysaccharide POJ-U1a extracted by ultrasound from Ophiopogon japonicus.

  2012cited by this paper
• Rational therapy for diabetes: early recognition of adverse effects and avoidance of disruptive false alarms

  2012cited by this paper
• Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus

  2011cited by this paper
• Sulfation of tea polysaccharides: synthesis, characterization and hypoglycemic activity.

  2010cited by this paper
• Isolation, chemical composition and antioxidant activities of a water-soluble polysaccharide from Cyclocarya paliurus (Batal.) Iljinskaja

  2010cited by this paper
• Induction of Apoptosis by Ethyl-acetate Fraction of Extracts from Tetrastigma Hemsleyanum Diels et.Gilg in Human Colon Cancer HT-29 Cells

  2010cited by this paper
• AMPK in Health and Disease.

  2009cited by this paper
• Glucokinase and molecular aspects of liver glycogen metabolism.

  2008cited by this paper
• Structural characterization of a water-soluble polysaccharide from the roots of Codonopsis pilosula and its immunity activity.

  2008cited by this paper
• Immunoregulatory effects of ethyl-acetate fraction of extracts from Tetrastigma hemsleyanum Diels et. Gilg on immune functions of ICR mice.

  2008cited by this paper
• Metformin Inhibits Hepatic Gluconeogenesis Through AMP-Activated Protein Kinase–Dependent Regulation of the Orphan Nuclear Receptor SHP

  2008cited by this paper
• Glucose-6-phosphatase flux in vitro is increased in type 2 diabetes.

  2000cited by this paper
• 5-aminoimidazole-4-carboxamide riboside mimics the effects of insulin on the expression of the 2 key gluconeogenic genes PEPCK and glucose-6-phosphatase.

  2000cited by this paper
• Loss of insulin signaling in hepatocytes leads to severe insulin resistance and progressive hepatic dysfunction.

  2000cited by this paper
• Induction of PEPCK gene expression in insulinopenia in rat small intestine.

  2000cited by this paper
• Avoiding oxidative degradation during sodium hydroxide/methyl iodide-mediated carbohydrate methylation in dimethyl sulfoxide

  1993cited by this paper
• Quantitative analysis by various g.l.c. response-factor theories for partially methylated and partially ethylated alditol acetates☆

  1975cited by this paper
• New method for quantitative determination of uronic acids.

  1973cited by this paper
• Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups.

  1972cited by this paper

• An Evaluation of Tetrastigma hemsleyanum Leaves: Digestion, Absorption and Anti-inflammatory Effects via an In Vitro Digestion-Caco-2-HT-29 Model

  2026cites this paper
• Structure-function relationship of polysaccharides derived from Pericarpium Citri Reticulatae 'Chachiensis': highlighting the effects on metabolic syndrome by regulating gut microbiota.

  2026cites this paper
• A Novel Polysaccharide Purified from Tricholoma matsutake: Structural Characterization and In Vitro Immunological Activity

  2025cites this paper
• Structural characterization and immunomodulatory activity analysis of a novel pectic polysaccharide extracted from Tetrastigma hemsleyanum Diels et Gilg and its hydrolysis products.

  2025cites this paper
• Physicochemical and colon cancer HT-29 cell inhibitory property of homogeneous polysaccharide from Stropharia rugosoannulata.

  2025cites this paper
• Recent advances in natural polysaccharides for type 2 diabetes management: sources, structural characteristics, and mechanistic insights

  2025cites this paper
• Novel oil-in-ionic liquid microemulsion extraction and in situ liquid–liquid microextraction of natural phenolic compounds from functional foods

  2025cites this paper
• The polysaccharides from mixed-culture fermentation of huangjing: extraction, structural analysis and anti-lipid activity

  2025cites this paper
• Novel and Potential Photoprotective and Tyrosinase Inhibitory Effects of Tetrastigma erubescens Extracts: Evidence from In Vitro Assays and Computational Approach

  2025cites this paper
• Systematic review of hypoglycemic polysaccharides: sources, preparation, and identification techniques, evaluation models, structure–activity relationships, and mechanistic insights

  2025cites this paper
• Exploring Tetrastigma hemsleyanum polysaccharides: A recent advance in preparation, structural features, bioactivities, and potential application prospects.

  2025cites this paper
• Structural Characterization and Bioactivity Analysis of Ultrasound‐Assisted Polysaccharides From Codonopsis pilosula: Insights Into Antioxidant and Hypoglycemic Potentials

  2025cites this paper
• Comparison of Nutritional and Medicinal Ingredients Between Ganoderma leucocontextum and G. lucidum

  2024cites this paper
• Protective effects of Nogo-B deficiency in NAFLD mice and its multiomics analysis of gut microbiology and metabolism

  2024cites this paper
• Shoot Organogenesis from Tetrastigma hemsleyanum Leaf and Petiole Explants, and Subsequent Plant Regeneration and Acclimatization

  2024cites this paper
• Molecular mechanisms of Tetrastigma hemsleyanum Diels&Gilg against lung squamous cell carcinoma: From computational biology and experimental validation.

  2024cites this paper
• Structural characterization of squash polysaccharide and its effect on STZ-induced diabetes mellitus model in MIN6 cells.

  2024cites this paper
• Superior hemostatic and wound-healing properties of tetrastigma polysaccharide

  2024cites this paper
• Kaempferol 3-O-Rutinoside, a Flavone Derived from Tetrastigma hemsleyanum Diels et Gilg, Reduces Body Temperature through Accelerating the Elimination of IL-6 and TNF-α in a Mouse Fever Model

  2024cites this paper
• Structural characterization and mechanisms of macrophage immunomodulatory activity of a novel polysaccharide with a galactose backbone from the processed Polygonati Rhizoma

  2024cites this paper
• A glucuronogalactomannan isolated from Tetrastigma hemsleyanum Diels et Gilg: Structure and immunomodulatory activity.

  2024cites this paper
• Effects of dual-strain fermentation on physicochemical properties of Rosa roxburghii Tratt and coix seed beverage

  2024cites this paper
• Advances in polysaccharides of natural source of anti-diabetes effect and mechanism

  2024cites this paper
• Advances in the study of polysaccharides from Anemarrhena asphodeloides Bge.: A review.

  2024cites this paper
• Structural characterization of cell-wall polysaccharides purified from chayote (Sechium edule) fruit

  2023cites this paper
• Optimizing extraction, structural characterization, and in vitro hypoglycemic activity of a novel polysaccharide component from Lentinus edodes

  2023cites this paper
• Polysaccharides from Tetrastigma Hemsleyanum Diels et Gilg ameliorated inflammatory bowel disease by rebuilding the intestinal mucosal barrier and inhibiting inflammation through the SCFA-GPR41/43 signaling pathway.

  2023cites this paper
• Structural characterization of a polysaccharide derived from Saposhnikovia divaricatee (Turcz.) Schischk with anti-allergic and antioxidant activities.

  2023cites this paper
• Fermentation-mediated variations in structure and biological activity of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg.

  2023cites this paper
• Physicochemical characterization, digestion profile and gut microbiota regulation activity of intracellular polysaccharides from Chlorella zofingiensis.

  2023cites this paper
• Structural characteristics and antioxidant and hypoglycemic activities of a heteropolysaccharide from Anemarrhena asphodeloides Bunge.

  2023cites this paper
• Immunoregulatory effects of Tetrastigma hemsleyanum polysaccharide via TLR4-mediated NF-κB and MAPK signaling pathways in Raw264.7 macrophages.

  2023cites this paper
• Tetrastigma polysaccharide reprogramming of tumor-associated macrophages via PPARγ signaling pathway to play antitumor activity in breast cancer.

  2023cites this paper
• Structural characterization of corn fiber hemicelluloses extracted by organic solvent and screening of degradation enzymes.

  2023cites this paper
• The stereoscopic planting mode improved the quality and yield of Tetrastigma hemsleyanum

  2023cites this paper
• Review on mechanisms and structure-activity relationship of hypoglycemic effects of polysaccharides from natural resources

  2023cites this paper
• Structural characteristics of mixed pectin from ginseng berry and its anti-obesity effects by regulating the intestinal flora.

  2023cites this paper
• Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng

  2023cites this paper
• Natural activators of AMPK signaling: potential role in the management of type-2 diabetes

  2022cites this paper
• Tetrastigma hemsleyanum alleviates sarcoidosis through metabolomic regulation and Th17/Treg immune homeostasis

  2022cites this paper
• Blood-Enriching Effects and Immune-Regulation Mechanism of Steam-Processed Polygonatum Sibiricum Polysaccharide in Blood Deficiency Syndrome Mice

  2022cites this paper
• The possible future changes in potential suitable habitats of Tetrastigma hemsleyanum (Vitaceae) in China predicted by an ensemble model

  2022cites this paper
• Genus Tetrastigma: A review of its folk uses, phytochemistry and pharmacology

  2022influential citation
• Seasonal variation influences flavonoid biosynthesis path and content, and antioxidant activity of metabolites in Tetrastigma hemsleyanum Diels & Gilg

  2022cites this paper
• Polysaccharide from Hovenia dulcis (Guaizao) improves pancreatic injury and regulates liver glycometabolism to alleviate STZ-induced type 1 diabetes mellitus in rats.

  2022cites this paper
• A Novel Polysaccharide From Heimioporus retisporus Displays Hypoglycemic Activity in a Diabetic Mouse Model

  2022cites this paper
• Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages.

  2022cites this paper
• Structural properties and bioactivities of pectic polysaccharides isolated from soybean hulls

  2022cites this paper
• Structural characteristics of polysaccharide from Zingiber striolatum and its effects on gut microbiota composition in obese mice

  2022cites this paper
• Salt interferences to metabolite accumulation, flavonoid biosynthesis and photosynthetic activity in Tetrastigma hemsleyanum

  2021cites this paper
• Inhibition of porcine epidemic diarrhea virus by Alpiniae oxyphyllae fructus polysaccharide 3.

  2021cites this paper
• A novel low-molecular-mass pumpkin polysaccharide: Structural characterization, antioxidant activity, and hypoglycemic potential.

  2021cites this paper
• Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity

  2021cites this paper
• Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.

  2021cites this paper
• Extract From Tetrastigma hemsleyanum Leaf Alleviates Pseudomonas aeruginosa Lung Infection: Network Pharmacology Analysis and Experimental Evidence

  2021cites this paper
• Structural characterization of polysaccharide from Centipeda minima and its hypoglycemic activity through alleviating insulin resistance of hepatic HepG2 cells

  2021cites this paper
• Machine learning classification of origins and varieties of Tetrastigma hemsleyanum using a dual-mode microscopic hyperspectral imager.

  2021cites this paper
• Ultra-flexible light-permeable organic solar cells for the herbal photosynthetic growth

  2021cites this paper
• Structural analysis and biological effects of a neutral polysaccharide from the fruits of Rosa laevigata.

  2021cites this paper
• Seasonal Variation of Biosynthetic Path Selectivity of Flavonoids, and Content and Antioxidant Activity of Metabolites in Tetrastigma Hemsleyanum Diels Et Gilg

  2021cites this paper
• Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy.

  2021cites this paper
• Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H2O2-induced oxidative damage in RAW 264.7 cells

  2021cites this paper
• The research progresses and future prospects of Tetrastigma hemsleyanum Diels et Gilg: A valuable Chinese herbal medicine.

  2021cites this paper
• Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum.

  2020cites this paper
• Superior antibacterial activity of sulfur-doped g-C3N4 nanosheets dispersed by Tetrastigma hemsleyanum Diels & Gilg's polysaccharides-3 solution.

  2020cites this paper
• Structural characterization and antioxidant activity of polysaccharides extracted from jujube using subcritical water

  2020cites this paper
• The Phytochemistry, Pharmacology, and Quality Control of Tetrastigma hemsleyanum Diels & Gilg in China: A Review

  2020cites this paper
• A comprehensive review on traditional uses, chemical compositions, pharmacology properties and toxicology of Tetrastigma hemsleyanum

  2020cites this paper
• Protective effect of the isolated oligosaccharide from Rosa canina in STZ-treated cells through modulation of the autophagy pathway.

  2020cites this paper
• Structural characteristics and anti-tumor/-oxidant activity in vitro of an acidic polysaccharide from Gynostemma pentaphyllum.

  2020cites this paper
• Novel polysaccharide from Chaenomeles speciosa seeds: Structural characterization, α-amylase and α-glucosidase inhibitory activity evaluation.

  2020cites this paper
• Purification, characterization and immunomodulatory activity of polysaccharides from Leccinum crocipodium (Letellier.) Watliag.

  2020cites this paper
• Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus.

  2019cites this paper
• The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum

  2019cites this paper
• Total flavonoids from Tetrastigma hemsleyanum ameliorates inflammatory stress in concanavalin A-induced autoimmune hepatitis mice by regulating Treg/Th17 immune homeostasis

  2019cites this paper
• Antibacterial mechanism of polysaccharide from Tetrastigma hemsleyanum Diels et Gilg's polysaccharides by metabolomics based on HPLC/MS.

  2019cites this paper
• Structural elucidation of an acidic polysaccharide from Citrus grandis 'Tomentosa' and its anti-proliferative effects on LOVO and SW620 cells.

  2019cites this paper
• Ultrafiltration isolation, hypoglycemic activity analysis and structural characterization of polysaccharides from Brasenia schreberi.

  2019cites this paper
• Hypoglycemic effects of edible fungus polysaccharides: A mini review

  year unknowncites this paper