HIV-1 vaccine design through minimizing envelope metastability

A coherent HIV-1 vaccine strategy addresses envelope stabilization, nanoparticle display, antibody response, and manufacture. Overcoming envelope metastability is crucial to trimer-based HIV-1 vaccine design. Here, we present a coherent vaccine strategy by minimizing metastability. For 10 strains across five clades, we demonstrate that the gp41 ectodomain (gp41ECTO) is the main source of envelope metastability by replacing wild-type gp41ECTO with BG505 gp41ECTO of the uncleaved prefusion-optimized (UFO) design. These gp41ECTO-swapped trimers can be produced in CHO cells with high yield and high purity. The crystal structure of a gp41ECTO-swapped trimer elucidates how a neutralization-resistant tier 3 virus evades antibody recognition of the V2 apex. UFO trimers of transmitted/founder viruses and UFO trimers containing a consensus-based ancestral gp41ECTO suggest an evolutionary root of metastability. The gp41ECTO-stabilized trimers can be readily displayed on 24- and 60-meric nanoparticles, with incorporation of additional T cell help illustrated for a hyperstable 60-mer, I3-01. In mice and rabbits, these gp140 nanoparticles induced tier 2 neutralizing antibody responses more effectively than soluble trimers.

• Publication year

  2018

• Venue

  Science Advances

• Publication date

  2018-07-04

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1101/361931PMID 30474059PMCID 6248932
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Improving the Expression and Purification of Soluble, Recombinant Native-Like HIV-1 Envelope Glycoprotein Trimers by Targeted Sequence Changes

  2017cited by this paper
• Structure-Based Design of a Soluble Prefusion-Closed HIV-1 Env Trimer with Reduced CD4 Affinity and Improved Immunogenicity

  2017cited by this paper
• Reducing V3 Antigenicity and Immunogenicity on Soluble, Native-Like HIV-1 Env SOSIP Trimers

  2017cited by this paper
• High-Throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers

  2017cited by this paper
• Stabilization of a soluble, native-like trimeric form of an efficiently cleaved Indian HIV-1 clade C envelope glycoprotein

  2017cited by this paper
• Tfh cells and HIV bnAbs, an immunodominance model of the HIV neutralizing antibody generation problem

  2017influential reference
• Soluble Prefusion Closed DS-SOSIP.664-Env Trimers of Diverse HIV-1 Strains.

  2017cited by this paper
• Differential Antibody Responses to Conserved HIV-1 Neutralizing Epitopes in the Context of Multivalent Scaffolds and Native-Like gp140 Trimers

  2017cited by this paper
• The quest for an antibody‐based HIV vaccine

  2017cited by this paper
• Native‐like Env trimers as a platform for HIV‐1 vaccine design

  2017cited by this paper
• Particulate Array of Well‐Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach

  2017cited by this paper
• A Broadly Neutralizing Antibody Targets the Dynamic HIV Envelope Trimer Apex via a Long, Rigidified, and Anionic β-Hairpin Structure

  2017cited by this paper
• Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches

  2017cited by this paper
• cGMP production and analysis of BG505 SOSIP.664, an extensively glycosylated, trimeric HIV‐1 envelope glycoprotein vaccine candidate

  2017cited by this paper
• The HIV‐1 envelope glycoprotein structure: nailing down a moving target

  2017cited by this paper
• Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein

  2017cited by this paper
• Open and Closed Structures Reveal Allostery and Pliability in the HIV-1 Envelope Spike

  2017cited by this paper
• Improving the Immunogenicity of Native-like HIV-1 Envelope Trimers by Hyperstabilization

  2017cited by this paper
• An accelerated rabies vaccine schedule based on toll-like receptor 3 (TLR3) agonist PIKA adjuvant augments rabies virus specific antibody and T cell response in healthy adult volunteers.

  2017cited by this paper
• Sequential and Simultaneous Immunization of Rabbits with HIV-1 Envelope Glycoprotein SOSIP.664 Trimers from Clades A, B and C

  2016cited by this paper
• Crystal structure , conformational fixation , and entry-related interactions of mature ligand-free HIV-1 Env

  2016cited by this paper
• Design of a hyperstable 60-subunit protein icosahedron

  2016cited by this paper
• Molecular Architecture of the Cleavage-Dependent Mannose Patch on a Soluble HIV-1 Envelope Glycoprotein Trimer

  2016cited by this paper
• Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer

  2016cited by this paper
• Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody.

  2016cited by this paper
• Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody

  2016cited by this paper
• Epitope-Independent Purification of Native-Like Envelope Trimers from Diverse HIV-1 Isolates

  2016cited by this paper
• Presenting native-like trimeric HIV-1 antigens with self-assembling nanoparticles

  2016cited by this paper
• Uncleaved prefusion-optimized gp140 trimers derived from analysis of HIV-1 envelope metastability

  2016cited by this paper
• HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies

  2016cited by this paper
• Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G.

  2016cited by this paper
• Holes in the Glycan Shield of the Native HIV Envelope Are a Target of Trimer-Elicited Neutralizing Antibodies.

  2016cited by this paper
• Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein

  2016cited by this paper
• Cleavage-independent HIV-1 Env trimers engineered as soluble native spike mimetics for vaccine design.

  2015influential reference
• HIV-1 neutralizing antibodies induced by native-like envelope trimers

  2015cited by this paper
• Single-Chain Soluble BG505.SOSIP gp140 Trimers as Structural and Antigenic Mimics of Mature Closed HIV-1 Env

  2015cited by this paper
• A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene

  2015cited by this paper
• Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity

  2015cited by this paper
• Structure-Guided Redesign Increases the Propensity of HIV Env To Generate Highly Stable Soluble Trimers

  2015cited by this paper
• New Member of the V1V2-Directed CAP256-VRC26 Lineage That Shows Increased Breadth and Exceptional Potency

  2015cited by this paper
• Crystal structure, conformational fixation, and entry-related interactions of mature ligand-free HIV-1 Env

  2015cited by this paper
• Murine Antibody Responses to Cleaved Soluble HIV-1 Envelope Trimers Are Highly Restricted in Specificity

  2015cited by this paper
• Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans.

  2015cited by this paper
• Antibody responses to envelope glycoproteins in HIV-1 infection

  2015cited by this paper
• Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens

  2015cited by this paper
• Immunogenicity of Stabilized HIV-1 Envelope Trimers with Reduced Exposure of Non-neutralizing Epitopes.

  2015cited by this paper
• Structural Constraints Determine the Glycosylation of HIV-1 Envelope Trimers.

  2015cited by this paper
• Structural delineation of a quaternary, cleavage-dependent epitope at the gp41-gp120 interface on intact HIV-1 Env trimers.

  2014cited by this paper
• Broadly neutralizing HIV antibodies define a glycan-dependent epitope on the prefusion conformation of gp41 on cleaved envelope trimers.

  2014influential reference
• Stable 293 T and CHO cell lines expressing cleaved, stable HIV-1 envelope glycoprotein trimers for structural and vaccine studies

  2014cited by this paper
• Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1.

  2014cited by this paper
• Faculty Opinions recommendation of Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer.

  2014cited by this paper
• Why HIV Virions Have Low Numbers of Envelope Spikes: Implications for Vaccine Development

  2014cited by this paper
• Structural evolution of glycan recognition by a family of potent HIV antibodies.

  2014influential reference
• Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex

  2014cited by this paper
• Generation of a universal CD4 memory T cell recall peptide effective in humans, mice and non-human primates.

  2014cited by this paper
• Antibody 8ANC195 reveals a site of broad vulnerability on the HIV-1 envelope spike.

  2014cited by this paper
• Broad and potent HIV-1 neutralization by a human antibody that binds the gp41-120 interface

  2014cited by this paper
• Cooperation of B cell lineages in induction of HIV-1-broadly neutralizing antibodies.

  2014cited by this paper
• Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus

  2013cited by this paper
• Antigenicity and Immunogenicity of Transmitted/Founder, Consensus, and Chronic Envelope Glycoproteins of Human Immunodeficiency Virus Type 1

  2013cited by this paper
• A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies

  2013cited by this paper
• Broadly Neutralizing Antibody PGT121 Allosterically Modulates CD4 Binding via Recognition of the HIV-1 gp120 V3 Base and Multiple Surrounding Glycans

  2013cited by this paper
• Phenotypic properties of transmitted founder HIV-1

  2013cited by this paper
• Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer

  2013cited by this paper
• Global Panel of HIV-1 Env Reference Strains for Standardized Assessments of Vaccine-Elicited Neutralizing Antibodies

  2013cited by this paper
• Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer

  2013cited by this paper
• Virus-like particles: the future of microbial factories and cell-free systems as platforms for vaccine development

  2013cited by this paper
• RNA-based vaccines.

  2012cited by this paper
• Anti-HIV B Cell Lines as Candidate Vaccine Biosensors

  2012cited by this paper
• Virus-like particles as a highly efficient vaccine platform: Diversity of targets and production systems and advances in clinical development

  2012cited by this paper
• Broad neutralization coverage of HIV by multiple highly potent antibodies

  2011cited by this paper
• DNA vaccines: an historical perspective and view to the future

  2011cited by this paper
• Memory, Visual Discrimination Performance, and the Human Hippocampus

  2011cited by this paper
• Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1

  2010cited by this paper
• Features and development of Coot

  2010cited by this paper
• MolProbity: all-atom structure validation for macromolecular crystallography

  2009cited by this paper
• Development of a single column method for the separation of lipid- and protein-derived oligosaccharides.

  2009cited by this paper
• Broad and Potent Neutralizing Antibodies from an African Donor Reveal a New HIV-1 Vaccine Target

  2009cited by this paper
• Tiered Categorization of a Diverse Panel of HIV-1 Env Pseudoviruses for Assessment of Neutralizing Antibodies

  2009cited by this paper
• DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy.

  2009cited by this paper
• Appion: an integrated, database-driven pipeline to facilitate EM image processing.

  2009cited by this paper
• Structural and quantitative analysis of N-linked glycans by matrix-assisted laser desorption ionization and negative ion nanospray mass spectrometry.

  2008cited by this paper
• The coming of age of virus-like particle vaccines

  2008cited by this paper
• A centralized gene-based HIV-1 vaccine elicits broad cross-clade cellular immune responses in rhesus monkeys

  2008cited by this paper
• Replicating and non-replicating viral vectors for vaccine development

  2007cited by this paper
• Virus-like particles—universal molecular toolboxes

  2007cited by this paper
• Exploiting the defensive sugars of HIV-1 for drug and vaccine design

  2007cited by this paper
• Phaser crystallographic software

  2007cited by this paper
• A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses.

  2006cited by this paper
• Ancestral and consensus envelope immunogens for HIV-1 subtype C.

  2006cited by this paper
• Neutralization Escape Variants of Human Immunodeficiency Virus Type 1 Are Transmitted from Mother to Infant

  2006cited by this paper
• Fragmentation of negative ions from carbohydrates: Part 2. Fragmentation of high-mannose N-linked glycans

  2005cited by this paper
• Automated molecular microscopy: the new Leginon system.

  2005cited by this paper
• Fragmentation of negative ions from carbohydrates: Part 3. Fragmentation of hybrid and complex N-linked glycans

  2005cited by this paper
• Molecular crowding enhances native state stability and refolding rates of globular proteins.

  2005cited by this paper
• Antigenicity and Immunogenicity of a Synthetic Human Immunodeficiency Virus Type 1 Group M Consensus Envelope Glycoprotein

  2005cited by this paper
• Fragmentation of negative ions from carbohydrates: Part 1. Use of nitrate and other anionic adducts for the production of negative ion electrospray spectra from N-linked carbohydrates

  2005cited by this paper
• UCSF Chimera—A visualization system for exploratory research and analysis

  2004cited by this paper
• research papers Acta Crystallographica Section D Biological

  2003cited by this paper

• Native-like soluble E1E2 glycoprotein heterodimers on self-assembling protein nanoparticles for hepatitis C virus vaccine design.

  2026cites this paper
• Signatures of native-like glycosylation in RNA replicon-derived HIV-1 immunogens.

  2026cites this paper
• Transmucosal delivery of a dendritic cell-targeting intranasal nanovaccine boosts HIV-1 specific mucosal and systemic immune responses.

  2026cites this paper
• Structure-guided design of native-like HIV Env Single Chain Trimers for enhanced stability, immunogenicity, and versatile vaccine delivery

  2025influential citation
• A multi-scale nanoparticle delivery system enhances neutralizing antibody responses in subunit vaccines

  2025cites this paper
• A Synthetic Liposome Nanoparticle Vaccine Platform for Broad-Spectrum Vaccine Design.

  2025cites this paper
• Stabilizing the Shield: C-Terminal Tail Mutation of HMPV F Protein for Enhanced Vaccine Design

  2025cites this paper
• Native-like soluble E1E2 glycoprotein heterodimers on self-assembling protein nanoparticles for hepatitis C virus vaccine design

  2025cites this paper
• Rational design of next-generation filovirus vaccines through glycoprotein stabilization, nanoparticle display, and glycan modification

  2025cites this paper
• Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV

  2025cites this paper
• The Design and Immunogenicity of an HIV-1 Clade C Pediatric Envelope Glycoprotein Stabilized by Multiple Platforms

  2025cites this paper
• Rational design of next-generation filovirus vaccines combining glycoprotein stabilization and nanoparticle display with glycan modification

  2025cites this paper
• Vaccine induction of CD4-mimicking HIV-1 broadly neutralizing antibody precursors in macaques.

  2024cites this paper
• Rational design of uncleaved prefusion-closed trimer vaccines for human respiratory syncytial virus and metapneumovirus

  2024influential citation
• Design and immunogenicity of an HIV-1 clade C pediatric envelope glycoprotein stabilized by multiple platforms

  2024cites this paper
• Development of a Two-Component Nanoparticle Vaccine Displaying an HIV-1 Envelope Glycoprotein that Elicits Tier 2 Neutralising Antibodies

  2024cites this paper
• Self-Assembled Ferritin Nanoparticles for Delivery of Antigens and Development of Vaccines: From Structure and Property to Applications

  2024cites this paper
• A Single-Component Multilayered Self-Assembling Protein Nanoparticle Vaccine Based on Extracellular Domains of Matrix Protein 2 against Both Influenza A and B

  2024cites this paper
• FcRider: a recombinant Fc nanoparticle with endogenous adjuvant activities for hybrid immunization

  2024cites this paper
• A nucleoside-modified mRNA vaccine forming rabies virus-like particle elicits strong cellular and humoral immune responses against rabies virus infection in mice

  2024cites this paper
• A Universal, Single-Component Multilayered Self-Assembling Protein Nanoparticle Vaccine Based on Extracellular Domains of Matrix Protein 2 Against Both Influenza A and B

  2024cites this paper
• Protein Nanoparticles as Vaccine Platforms for Human and Zoonotic Viruses

  2024cites this paper
• Membrane HIV-1 envelope glycoproteins stabilized more strongly in a pretriggered conformation than natural virus Envs

  2024cites this paper
• In vivo affinity maturation of mouse B cells reprogrammed to express human antibodies

  2024cites this paper
• A tale of two fusion proteins: understanding the metastability of human respiratory syncytial virus and metapneumovirus and implications for rational design of uncleaved prefusion-closed trimers

  2024cites this paper
• Microsecond dynamics control the HIV-1 Envelope conformation

  2024cites this paper
• Ferritin-based nanomedicine for disease treatment

  2023cites this paper
• Virus-mimicking nanosystems: from design to biomedical applications.

  2023cites this paper
• In vivo affinity maturation of murine B cells reprogrammed to express human antibodies

  2023cites this paper
• Vaccination with immune complexes modulates the elicitation of functional antibodies against HIV-1

  2023cites this paper
• Protein-Based Nanocarriers and Nanotherapeutics for Infection and Inflammation

  2023cites this paper
• Enhancement of Neutralization Responses through Sequential Immunization of Stable Env Trimers Based on Consensus Sequences from Select Time Points by Mimicking Natural Infection

  2023influential citation
• Single-Component Multilayered Self-Assembling Protein Nanoparticles Displaying Extracellular Domains of Matrix Protein 2 as a Pan-influenza A Vaccine

  2023influential citation
• Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimers as HIV-1 vaccine candidates

  2023cites this paper
• Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern

  2023cites this paper
• Microsecond dynamics control the HIV-1 envelope conformation

  2023cites this paper
• Fusion of the molecular adjuvant C3d to cleavage-independent native-like HIV-1 Env trimers improves the elicited antibody response

  2023influential citation
• Improvement of B Cell Responses by an HIV-1 Amphiphilic Polymer Nanovaccine.

  2023cites this paper
• Protein-based nano-vaccines against SARS-CoV-2: Current design strategies and advances of candidate vaccines

  2023cites this paper
• Vaccine induction of CD4-mimicking broadly neutralizing antibody precursors in macaques

  2023cites this paper
• Development of a synthetic nanoparticle vaccine presenting the HIV-1 envelope glycoprotein

  2022cites this paper
• Hemagglutinin‐based DNA vaccines containing trimeric self‐assembling nanoparticles confer protection against influenza

  2022cites this paper
• Induction of tier-2 neutralizing antibodies in mice with a DNA-encoded HIV envelope native like trimer

  2022cites this paper
• Structure-guided changes at the V2 apex of HIV-1 clade C trimer enhance elicitation of autologous neutralizing and broad V1V2-scaffold antibodies

  2022cites this paper
• A neutralizing antibody target in early HIV-1 infection was recapitulated in rhesus macaques immunized with the transmitted/founder envelope sequence

  2022cites this paper
• C3-Symmetric Aromatic Core of Griffithsin Is Essential for Potent Anti-HIV Activity.

  2022cites this paper
• Covalent binding of human two-domain CD4 to an HIV-1 subtype C SOSIP.664 trimer modulates its structural dynamics.

  2022cites this paper
• Improving cross-protection against influenza virus in mice using a nanoparticle vaccine of mini-HA.

  2022cites this paper
• Computational design of vaccine immunogens.

  2022cites this paper
• Pan-neutralizing, germline-encoded antibodies against SARS-CoV-2: Addressing the long-term problem of escape variants

  2022influential citation
• Induction of tier-2 neutralizing antibodies in mice with a DNA-encoded HIV envelope native like trimer

  2022cites this paper
• Heavy-chain CDR3-engineered B cells facilitate in vivo evaluation of HIV-1 vaccine candidates

  2022cites this paper
• Single-component multilayered self-assembling protein nanoparticles 1 presenting glycan-trimmed uncleaved prefusion optimized envelope trimers 2 as HIV-1 vaccine candidates 3 4

  2022influential citation
• Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants

  2021cites this paper
• Advancing the development of therapeutics against infectious diseases with optimized protein expression systems

  2021cites this paper
• Disassembly of HIV envelope glycoprotein trimer immunogens is driven by antibodies elicited via immunization

  2021cites this paper
• HIV vaccinology: 2021 update.

  2021cites this paper
• HIV-1 Envelope Conformation, Allostery, and Dynamics

  2021cites this paper
• Protein-based antigen presentation platforms for nanoparticle vaccines

  2021cites this paper
• Structure-based design of a highly stable, covalently-linked SARS-CoV-2 spike trimer with improved structural properties and immunogenicity

  2021influential citation
• Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants

  2021cites this paper
• Single-component, self-assembling, protein nanoparticles presenting the receptor binding domain and stabilized spike as SARS-CoV-2 vaccine candidates

  2021cites this paper
• Two dimensional nanosheets as immunoregulator improve HIV vaccine efficacy

  2021cites this paper
• Exploration of a Sequential Gp140-Gp145 Immunization Regimen with Heterologous Envs to Induce a Protective Cross-Reactive HIV Neutralizing Antibody Response In Non-human Primates

  2021cites this paper
• Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

  2021cites this paper
• Functionalizing Ferritin Nanoparticles for Vaccine Development

  2021cites this paper
• [Progress in HIV-1 Env trimer design].

  2020influential citation
• Biomimetic Nanomaterial Strategies for Virus Targeting: Antiviral Therapies and Vaccines

  2020cites this paper
• Neutralizing Antibodies Induced by First-Generation gp41-Stabilized HIV-1 Envelope Trimers and Nanoparticles

  2020influential citation
• Automated Design by Structure-Based Stabilization and Consensus Repair to Achieve Prefusion-Closed Envelope Trimers in a Wide Variety of HIV Strains.

  2020cites this paper
• A VH1-69 antibody lineage from an infected Chinese donor potently neutralizes HIV-1 by targeting the V3 glycan supersite

  2020cites this paper
• Single-component, self-assembling, protein nanoparticles presenting the receptor binding domain and stabilized spike as SARS-CoV-2 vaccine candidates

  2020influential citation
• Single-component multilayered self-assembling nanoparticles presenting rationally designed glycoprotein trimers as Ebola virus vaccines

  2020cites this paper
• Incorporation of a Novel CD4+ Helper Epitope Identified from Aquifex aeolicus Enhances Humoral Responses Induced by DNA and Protein Vaccinations

  2020cites this paper
• Controlling the SARS-CoV-2 spike glycoprotein conformation

  2020cites this paper
• Impact of the expression system on the immune responses to self-assembling protein nanoparticles (SAPNs) displaying HIV-1 V1V2 loop.

  2020cites this paper
• Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

  2020cites this paper
• Proof of concept for rational design of hepatitis C virus E2 core nanoparticle vaccines

  2020influential citation
• Controlling the SARS-CoV-2 Spike Glycoprotein Conformation

  2020cites this paper
• Characterization and Immunogenicity of HIV Envelope gp140 Zera® Tagged Antigens

  2020cites this paper
• Stabilized diverse HIV-1 envelope trimers for vaccine design

  2020influential citation
• Structural and functional evaluation of de novo-designed, two-component nanoparticle carriers for HIV Env trimer immunogens

  2020cites this paper
• Specific Substitutions in Region V2 of gp120 env confer SHIV Neutralisation Resistance

  2020cites this paper
• Presentation of HIV-1 envelope glycoprotein trimers on diverse nanoparticle platforms

  2019cites this paper
• Virus-Like Particles and Nanoparticles for Vaccine Development against HCMV

  2019cites this paper
• Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements

  2019cites this paper
• Nanoparticle Vaccines for Inducing HIV-1 Neutralizing Antibodies

  2019influential citation
• Neutralizing Antibody Induction by HIV-1 Envelope Glycoprotein SOSIP Trimers on Iron Oxide Nanoparticles May Be Impaired by Mannose Binding Lectin

  2019cites this paper
• Protein engineering and particulate display of B-cell epitopes to facilitate development of novel vaccines.

  2019cites this paper
• Broadly neutralizing antibodies and vaccine design against HIV-1 infection

  2019cites this paper
• HIV-1 Envelope Glycan Composition as a Key Determinant of Efficient Virus Transmission via DC-SIGN and Resistance to Inhibitory Lectins

  2019cites this paper
• Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice

  2019cites this paper
• Rational design of hepatitis C virus E2 core nanoparticle vaccines

  2019cites this paper