Chromatin-mediated depression of fractionation performance on electronegative multimodal chromatography media, its prevention, and ramifications for purification of immunoglobulin G.

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Journal of Chromatography A

• Publication date

  2014-12-29

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.chroma.2014.11.052PMID 25481347
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Nonspecific interactions of chromatin with immunoglobulin G and protein A, and their impact on purification performance.

  2014cited by this paper
• Evaluation of high‐capacity cation exchange chromatography for direct capture of monoclonal antibodies from high‐titer cell culture processes

  2014cited by this paper
• Evaluation of a multimodal resin for selective capture of CHO-derived monoclonal antibodies directly from harvested cell culture fluid.

  2014cited by this paper
• Characterization and removal of aggregates formed by nonspecific interaction of IgM monoclonal antibodies with chromatin catabolites during cell culture production.

  2013cited by this paper
• Void exclusion of antibodies by grafted-ligand porous particle anion exchangers.

  2013cited by this paper
• A model mechanism for protein precipitation by caprylic acid: Application to plasma purification

  2012cited by this paper
• Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification

  2012cited by this paper
• IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines.

  2012cited by this paper
• Chromatographic behavior of IgM:DNA complexes.

  2011cited by this paper
• The effects of hitchhiker antigens co-eluting with affinity-purified research antibodies.

  2011cited by this paper
• Cation-exchange chromatography of monoclonal antibodies: Characterisation of a novel stationary phase designed for production-scale purification

  2010cited by this paper
• Interpreting protein/DNA interactions: distinguishing specific from non-specific and electrostatic from non-electrostatic components

  2010cited by this paper
• Multifunctionality of the linker histones: an emerging role for protein-protein interactions

  2010cited by this paper
• Comparison of 7 methods for extracting cell-free DNA from serum samples of colorectal cancer patients.

  2009cited by this paper
• Histone H2AX stabilizes broken DNA strands to suppress chromosome breaks and translocations during V(D)J recombination

  2009cited by this paper
• Evicting hitchhiker antigens from purified antibodies.

  2009cited by this paper
• Advances in Technology and Process Development for Industrial‐Scale Monoclonal Antibody Purification

  2008cited by this paper
• Behavior of the Inadvertent pH Transient Formed by a Salt Gradient in the Ion‐Exchange Chromatography of Proteins

  2008cited by this paper
• Protein separations with induced pH gradients using cation-exchange chromatographic columns containing weak acid groups.

  2008cited by this paper
• Host cell protein clearance during protein a chromatography: Development of an improved column wash step

  2008cited by this paper
• An exclusion mechanism in ion exchange chromatography.

  2006cited by this paper
• Beyond the walls of the nucleus: the role of histones in cellular signaling and innate immunity.

  2006cited by this paper
• Profiling of medium chain glycerides used in pharmaceutical formulation development by reversed-phase HPLC.

  2006cited by this paper
• Chromatographic media for bioseparation.

  2005cited by this paper
• Extranuclear detection of histones and nucleosomes in activated human lymphoblasts as an early event in apoptosis

  2004cited by this paper
• Chromatin Specificity of Anti-Double-Stranded DNA Antibodies and a Role for Arg Residues in the Third Complementarity-Determining Region of the Heavy Chain 1

  2003cited by this paper
• Low pH, caprylate incubation as a second viral inactivation step in the manufacture of albumin. Parametric and validation studies.

  2003cited by this paper
• pH Transitions in Ion‐Exchange Systems: Role in the Development of a Cation‐Exchange Process for a Recombinant Protein

  2002cited by this paper
• Enveloped virus inactivation by caprylate: a robust alternative to solvent-detergent treatment in plasma derived intermediates.

  2002cited by this paper
• A Receptor Presentation Hypothesis for T Cell Help That Recruits Autoreactive B Cells1

  2001cited by this paper
• Amino acid?base interactions: a three-dimensional analysis of protein?DNA interactions at an atomic level

  2001cited by this paper
• Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody: structural basis for recognition of B-cell receptors and superantigen activity.

  2000cited by this paper
• Isolation and characterization of apoptotic nucleosomes, free and complexed with lupus autoantibody generated during hybridoma B-cell apoptosis.

  1998cited by this paper
• DNA binding within the nucleosome core.

  1998cited by this paper
• Crystal structure of the nucleosome core particle at 2.8 Å resolution

  1997cited by this paper
• Successive elution by ion-exchange chromatography of H3-H4 histone complexes differing in their degree of acetylation.

  1996cited by this paper
• An early post-mutational selection event directs expansion of autoreactive B cells in murine lupus.

  1995cited by this paper
• Endonuclease activities, chromatin structure, and DNA degradation in apoptosis.

  1994cited by this paper
• The anti‐La response of a single MRL/Mp‐lpr/lpr mouse: Specificity for DNA and VH gene usage

  1994cited by this paper
• Inactivation of Lipid‐Enveloped Viruses in Proteins by Caprylate

  1991cited by this paper
• Crystallographic structure of the octameric histone core of the nucleosome at a resolution of 3.3 A.

  1985cited by this paper
• Interactions of the nucleosomal core histones: a calorimetric study of octamer assembly

  1984cited by this paper
• Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.

  1982cited by this paper
• Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.

  1982cited by this paper
• Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution.

  1981cited by this paper
• Reversible association of calf thymus histones to form the symmetrical octamer (H2AH2BH3H4)2: a case of a mixed-associating system.

  1980cited by this paper
• Characterization of the octamer of histones free in solution.

  1977cited by this paper
• Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.

  1976cited by this paper
• Heterogeneity of chromatin subunits in vitro and location of histone H1.

  1976cited by this paper
• Some physiochemical properties of protein A from Staphylococcus aureus.

  1972cited by this paper
• Some Physicochemical Properties of Protein A from Staphylococcus aureus

  1972cited by this paper
• Interaction of lysine-rich histones and DNA.

  1969cited by this paper

• Multimodal Chromatography in the Downstream Processing of mAb-Based Products: Mechanisms, Strategies, and Applications.

  2026cites this paper
• Deciphering the inconsistent performance of Protein A membrane on host cell protein clearance.

  2026cites this paper
• The advantage of high pH eluting Protein A resins over their regular counterparts in aggregate and host cell protein clearance.

  2025cites this paper
• Factors affecting product association as a mechanism of host‐cell protein persistence in bioprocessing

  2024cites this paper
• The downstream purification of bispecific antibodies.

  2024cites this paper
• Host cell protein networks as a novel co‐elution mechanism during protein A chromatography

  2024cites this paper
• Depth filter material process interaction in the harvest of mammalian cells

  2023cites this paper
• Biomimetic affinity chromatography for antibody purification: Host cell protein binding and impurity removal.

  2023cites this paper
• Behavior of host-cell-protein-rich aggregates in antibody capture and polishing chromatography.

  2023cites this paper
• Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions

  2023cites this paper
• Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact.

  2022cites this paper
• Multiple-Monitor HPLC Assays for Rapid Process Development, In-Process Monitoring, and Validation of AAV Production and Purification

  2021cites this paper
• Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells

  2021cites this paper
• Polymer-Coated Fiber Optic Sensor as a Process Analytical Tool for Biopharmaceutical Impurity Detection

  2020cites this paper
• Enhancing Protein A performance in mAb processing: A method to reduce and rapidly evaluate host cell DNA levels during primary clarification

  2019cites this paper
• The application of caprylic acid in downstream processing of monoclonal antibodies.

  2019cites this paper
• Reduction of chromatin heteroaggregates by acid precipitation of mammalian cell culture and ramification in protein A chromatography for recombinant immunoglobulin G purification

  2019cites this paper
• Advance chromatin extraction improves the performance of electropositive mixed-mode chromatography as a capture step and enables its integration with void-exclusion anion exchange chromatography as a two-column-step purification platform for monoclonal antibody production

  2019cites this paper
• Non-affinity purification of a nanobody by void-exclusion anion exchange chromatography and multimodal weak cation exchange chromatography

  2019cites this paper
• A seamless and nonprotein A‐based monoclonal antibody purification platform using void‐exclusion anion exchange chromatography and electropositive multimodal chromatography enhanced by advance chromatin extraction

  2019cites this paper
• Chromatin-directed clarification in cell culture fluid enables non-protein affinity antibody purification by tangential flow filtration integrated with high-capacity cation exchange chromatography

  2019cites this paper
• Salt-enhanced permeabilization for monoclonal antibody precipitation and purification in a tubular reactor with a depth filtration membrane with advanced chromatin extraction

  2019cites this paper
• Integrated purification of a nanobody using ammonium sulfate precipitation and Capto MMC

  2019cites this paper
• Protein A chromatography resin lifetime—impact of feed composition

  2018cites this paper
• Host cell protein removal from biopharmaceutical preparations: Towards the implementation of quality by design.

  2018cites this paper
• Process development in the Quality by Design paradigm: Modeling of Protein A chromatography resin fouling.

  2018cites this paper
• Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification

  2018cites this paper
• Steric exclusion chromatography for purification of cell culture-derived influenza A virus using regenerated cellulose membranes and polyethylene glycol.

  2017cites this paper
• [Improved protein-A chromatography for monoclonal antibody purification].

  2016influential citation
• Characterization of the co‐elution of host cell proteins with monoclonal antibodies during protein A purification

  2016cites this paper
• Advance chromatin extraction enhances performance and productivity of cation exchange chromatography-based capture of Immunoglobulin G monoclonal antibodies.

  2016cites this paper
• Advance chromatin extraction improves capture performance of protein A affinity chromatography.

  2016cites this paper
• esidual on column host cell protein analysis during lifetime studies f protein A chromatography

  2016cites this paper
• Residual on column host cell protein analysis during lifetime studies of protein A chromatography.

  2016cites this paper
• Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism

  2016cites this paper
• A simple and efficient purification platform for monoclonal antibody production based on chromatin-directed cell culture clarification integrated with precipitation and void-exclusion anion exchange chromatography.

  2016cites this paper
• Challenges of glycosylation analysis and control: an integrated approach to producing optimal and consistent therapeutic drugs.

  2016cites this paper
• Non-immunospecific association of immunoglobulin G with chromatin during elution from protein A inflates host contamination, aggregate content, and antibody loss.

  2015cites this paper
• Characterization of DNA in cell culture supernatant by fluorescence-detection size-exclusion chromatography

  2015cites this paper