MS3 eliminates ratio distortion in isobaric labeling-based multiplexed quantitative proteomics

Quantitative mass spectrometry–based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry–based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) almost completely eliminates interference.

• Publication year

  2011

• Venue

  Nature Methods

• Publication date

  2011-09-20

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/nmeth.1714PMID 21963607PMCID 3205343
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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