New CRISPR‐Cas9 vectors for genetic modifications of Bacillus species

&NA; Genetic manipulation is a fundamental procedure for the study of gene and operon functions and new characteristics acquisition. Modern CRISPR‐Cas technology allows genome editing more precisely and increases the efficiency of transferring mutations in a variety of hard to manipulate organisms. Here, we describe new CRISPR‐Cas vectors for genetic modifications in bacillary species. Our plasmids are single CRISPR‐Cas plasmids comprising all components for genome editing and should be functional in a broad host range. They are highly efficient (up to 97%) and precise. The employment and delivery of these plasmids to bacillary strains can be easily achieved by conjugation from Escherichia coli. During our research we also demonstrated the absence of compatibility between CRISPR‐Cas system and non‐homologous end joining in Bacillus subtilis.

• Publication year

  2018

• Venue

  FEMS Microbiology Letters

• Publication date

  2018-12-06

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1093/femsle/fny284PMID 30520985
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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