CRISPR Craft: DNA Editing the Reconstructive Ladder

Summary: The clustered regularly interspaced short palindromic repeats (CRISPR) system of genome editing represents a major technological advance spanning all areas of genetics and downstream applications. CRISPR’s potential impact on treating human disease encompasses all clinical specialties, including areas important to the plastic surgeon such as oncology, wound healing, immunology, and craniofacial malformations. Plastic surgeons should gain familiarity with this gene editing technology, and become active contributors and leaders in applying CRISPR to their respective areas of expertise. This review describes the history and basic mechanism of CRISPR genome editing, highlights current and future applications, and discusses limitations. The authors will consider CRISPR’s potential impact and use in plastic and reconstructive surgery.

• Publication year

  2018

• Venue

  Plastic and Reconstructive Surgery

• Publication date

  2018-11-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.1097/PRS.0000000000004863PMID 30511992
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Making the cut

  2020cited by this paper
• Application of the gene editing tool, CRISPR‐Cas9, for treating neurodegenerative diseases

  2018cited by this paper
• Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

  2017cited by this paper
• Human Germline Genome Editing.

  2017cited by this paper
• RNA editing with CRISPR-Cas13

  2017cited by this paper
• The potential application and challenge of powerful CRISPR/Cas9 system in cardiovascular research.

  2017cited by this paper
• Utility of CRISPR/Cas9 systems in hematology research.

  2017cited by this paper
• Abstract 101: Nanoparticle Delivery of Keap1 siRNA Accelerates Diabetic Wound Healing

  2017cited by this paper
• One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs

  2017cited by this paper
• In Vivo Delivery of CRISPR/Cas9 for Therapeutic Gene Editing: Progress and Challenges.

  2017cited by this paper
• Of mice and CRISPR

  2017cited by this paper
• The Advent of Vascularized Composite Allotransplantation.

  2017cited by this paper
• The effect of age on microsurgical free flap outcomes: An analysis of 5,951 cases

  2017cited by this paper
• What rheumatologists need to know about CRISPR/Cas9

  2017cited by this paper
• SMCHD1 mutations associated with a rare muscular dystrophy can also cause isolated arhinia and Bosma arhinia microphthalmia syndrome

  2017cited by this paper
• The Resurgence of Xenotransplantation

  2017cited by this paper
• In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models.

  2017cited by this paper
• In Vivo Genome Editing Restores Dystrophin Expression and Cardiac Function in Dystrophic Mice

  2017cited by this paper
• Zebrafish models of orofacial clefts

  2017cited by this paper
• Engineered Epidermal Progenitor Cells Can Correct Diet-Induced Obesity and Diabetes.

  2017cited by this paper
• Molecular tumor targeting of gelonin by fusion with F3 peptide

  2017cited by this paper
• CRISPR/Cas9: at the cutting edge of hepatology

  2017cited by this paper
• Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis

  2016cited by this paper
• Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification

  2016cited by this paper
• Novel human mutation and CRISPR/Cas genome-edited mice reveal the importance of C-terminal domain of MSX1 in tooth and palate development

  2016cited by this paper
• CRISPR gene-editing tested in a person for the first time

  2016cited by this paper
• Chinese scientists to pioneer first human CRISPR trial

  2016cited by this paper
• Hair follicle aging is driven by transepidermal elimination of stem cells via COL17A1 proteolysis

  2016cited by this paper
• Golgb1 regulates protein glycosylation and is crucial for mammalian palate development

  2016cited by this paper
• In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy

  2016cited by this paper
• Gene therapy strategies to improve strength and quality of flexor tendon healing

  2016cited by this paper
• Application of CRISPR / Cas to the treatment of eye disease

  2016cited by this paper
• Zebrafish Genome Engineering Using the CRISPR-Cas9 System.

  2016cited by this paper
• Gene therapies that restore dystrophin expression for the treatment of Duchenne muscular dystrophy

  2016cited by this paper
• Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editing

  2016cited by this paper
• Research Techniques Made Simple: The Application of CRISPR-Cas9 and Genome Editing in Investigative Dermatology.

  2016cited by this paper
• Safety and Wound Outcomes Following Genetically Corrected Autologous Epidermal Grafts in Patients With Recessive Dystrophic Epidermolysis Bullosa.

  2016cited by this paper
• In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

  2016cited by this paper
• Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons

  2016cited by this paper
• CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

  2015cited by this paper
• Inducible in vivo genome editing with CRISPR/Cas9

  2015cited by this paper
• Excavating the Genome: Large-Scale Mutagenesis Screening for the Discovery of New Mouse Models.

  2015cited by this paper
• 365 days: The science events that shaped 2015

  2015cited by this paper
• Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery

  2015cited by this paper
• Adenovirally Delivered Enzyme Prodrug Therapy with Herpes Simplex Virus–Thymidine Kinase in Composite Tissue Free Flaps Shows Therapeutic Efficacy in Rat Models of Glioma

  2015cited by this paper
• A light-inducible CRISPR/Cas9 system for control of endogenous gene activation

  2015cited by this paper
• Utilization of a genetically modified muscle flap for local BMP-2 production and its effects on bone healing: a histomorphometric and radiological study in a rat model

  2015cited by this paper
• From Bench to Bedside and Back: Improving Diagnosis and Treatment of Craniofacial Malformations Utilizing Animal Models.

  2015cited by this paper
• Clinical Safety and Preliminary Efficacy of Plasmid pUDK-HGF Expressing Human Hepatocyte Growth Factor (HGF) in Patients with Critical Limb Ischemia.

  2015cited by this paper
• Premise and promise of mesenchymal stem cell-based therapies in clinical vascularized composite allotransplantation

  2015cited by this paper
• Breakthrough to genome editing

  2015cited by this paper
• Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy

  2015cited by this paper
• Making the cut.

  2015cited by this paper
• A guide to genome engineering with programmable nucleases

  2014cited by this paper
• The special case of gene therapy pricing

  2014cited by this paper
• Efficient In Vivo Genome Editing Using RNA-Guided Nucleases

  2013cited by this paper
• The Role of Gene Therapy in Regenerative Surgery: Updated Insights

  2013cited by this paper
• RNA-Guided Human Genome Engineering via Cas9

  2013cited by this paper
• Efficient In Vivo Genome Editing Using RNA-Guided Nucleases

  2013cited by this paper
• Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9

  2013cited by this paper
• Multiplex Genome Engineering Using CRISPR/Cas Systems

  2013cited by this paper
• CRISPR-mediated adaptive immune systems in bacteria and archaea.

  2013cited by this paper
• Phase I/IIa Clinical Trial of Therapeutic Angiogenesis Using Hepatocyte Growth Factor Gene Transfer to Treat Critical Limb Ischemia

  2011cited by this paper
• The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

  2010cited by this paper
• Safety and efficacy of patient specific intramuscular injection of HGF plasmid gene therapy on limb perfusion and wound healing in patients with ischemic lower extremity ulceration: results of the HGF-0205 trial.

  2010cited by this paper
• CRISPR/Cas, the Immune System of Bacteria and Archaea

  2010cited by this paper
• Treating Chronic Wound Infections with Genetically Modified Free Flaps

  2009cited by this paper
• Plastic Surgical Delivery Systems for Targeted Gene Therapy

  2008cited by this paper
• Using Genetically Modified Microvascular Free Flaps to Deliver Local Cancer Immunotherapy with Minimal Systemic Toxicity

  2008cited by this paper
• Gene therapy and wound healing.

  2007cited by this paper
• Gene therapy in wound healing: present status and future directions

  2007cited by this paper
• Gene therapy in plastic and reconstructive surgery.

  2005cited by this paper
• Gene therapy in wound repair and regeneration

  2000cited by this paper
• Gene transfer in wound healing.

  2000cited by this paper
• Gene therapy for plastic and reconstructive surgery.

  1996cited by this paper
• Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product

  1987cited by this paper

• Regarding "A Comprehensive Flap Classification: Beyond the Reconstructive Ladder".

  2025cites this paper
• Immortality Reconsidered: Clinical Challenges at the Frontier of Plastic Surgery

  2025cites this paper
• Skin Substitutes: Filling the Gap in the Reconstructive Algorithm

  2024cites this paper
• The increasingly imporant role of genetics in plastic surgery.

  2024cites this paper
• Molecular scalpels: the future of pediatric craniofacial surgery?

  2023cites this paper
• Innovations in Skin and Soft Tissue Aging—A Systematic Literature Review and Market Analysis of Therapeutics and Associated Outcomes

  2023cites this paper
• The Reconstructive Toolbox

  2023cites this paper
• Congratulations to the new role of Archives of Craniofacial Surgery as the official journal of Asian Pacific Craniofacial Association

  2022cites this paper
• SYSTEMATIC REVIEW Ethical Issues in Tissue Engineering: A Systematic Review

  2022cites this paper
• CRISPR-Cas9: el debate bioético más allá de la línea germinal

  2022cites this paper
• Aging, Skeletal Muscle, and Epigenetics.

  2021cites this paper
• Regenerative Engineering: Current Applications and Future Perspectives

  2021cites this paper
• Reconstruction 2.0: restructuring the reconstruction ladder.

  2020cites this paper
• A Genetic Makeover: Motives for Self-Enhancement with CRISPR

  2020cites this paper
• Role of Modern Technologies in Tissue Engineering

  2020cites this paper
• Engineering a Future with VCA: Applying Genetic Circuits to Engineer Tissues for Vascularized Composite Allotransplantation.

  2020cites this paper
• Bioethical issues in genome editing by CRISPR-Cas9 technology

  2020influential citation
• Discussion on Vascular Malformations: Current Progress Toward Drug Therapy

  2020cites this paper
• Utilising multiplex PCR technology for rapid microbial diagnosis in hand and upper limb infections

  2020cites this paper
• Gene therapy in wound healing using nanotechnology

  2020cites this paper
• A Prospective, Randomized, Blinded Trial Comparing Digital Simulation to Textbook for Cleft Surgery Education.

  2019cites this paper
• The Evolving Landscape of Gene Therapy in Plastic Surgery

  2019cites this paper
• Applied Bioengineering in Tissue Reconstruction, Replacement, and Regeneration

  2019cites this paper
• REPLY TO: CRISPR/Cas9-Mediated Genomic Editing: Implications for Engineering an Animal Model of Breast Implant-Associated Anaplastic Large Cell Lymphoma.

  2019cites this paper
• Reply: Delayed Postconditioning with External Volume Expansion Improves Survival of Adipose Tissue Grafts in a Murine Model.

  2019cites this paper
• Genetic Editing in Plastic Surgery.

  2019cites this paper
• CRISPR/Cas9-Mediated Genomic Editing: Implications for Engineering an Animal Model of Breast Implant-Associated Anaplastic Large Cell Lymphoma.

  2019cites this paper
• Discussion

  2018cites this paper
• Discussion: CRISPR Craft: DNA Editing the Reconstructive Ladder.

  2018cites this paper