New high-throughput technologies of post genome era: benefits and ethical issues

RESUMO: As areas da biologia celular e molecular e da genetica humana sofreram recentemente uma grande revolucao que trouxe novo conhecimento e novas tecnologias. Os estudos de sequenciacao em larga escala e de caracterizacao funcional permitem a monitorizacao simultânea de milhares de sequencias e moleculas, gerando uma grande quantidade de dados e fornecendo detalhes moleculares em tempo real e de modo instantâneo de um individuo. Nesta revisao faz-se uma referencia breve a algumas destas novas tecnicas, incluindo os metodos de sequenciacao de next-generation e os metodos de edicao de genes baseados no sistema CRISPR/Cas9. Sao apresentadas as potenciais aplicacoes e implicacoes destas novas tecnologias nas areas de investigacao e diagnostico molecular. Sao tambem discutidas as questoes eticas levantadas pela implementacao destas metodologias num futuro proximo.

• Publication year

  2017

• Venue

  Saúde & Tecnologia

• Publication date

  2017-08-07

• Fields of study

  Not labeled

• Identifiers
  DOI 10.25758/set.1661
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Microbiota Analysis Using an Illumina MiSeq Platform to Sequence 16S rRNA Genes

  2017cited by this paper
• Next-Generation Sequencing in Oncology: Genetic Diagnosis, Risk Prediction and Cancer Classification

  2017cited by this paper
• Comprehensive genotyping in dyslipidemia: mendelian dyslipidemias caused by rare variants and Mendelian randomization studies using common variants

  2017cited by this paper
• Next generation sequencing: implications in personalized medicine and pharmacogenomics.

  2016cited by this paper
• Genome Wide Sampling Sequencing for SNP Genotyping: Methods, Challenges and Future Development

  2016cited by this paper
• CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice

  2015cited by this paper
• Ethical, legal and social implications of incorporating personalized medicine into healthcare.

  2015cited by this paper
• Trend of different molecular markers in the last decades for studying human migrations.

  2015cited by this paper
• CRISPR, the disruptor

  2015cited by this paper
• Whole-genome sequencing of bladder cancers reveals somatic CDKN1A mutations and clinicopathological associations with mutation burden

  2014cited by this paper
• Next Generation Sequencing Reveals Skewing of the T and B Cell Receptor Repertoires in Patients with Wiskott–Aldrich Syndrome

  2014cited by this paper
• RNA Sequencing Reveals Upregulation of RUNX1-RUNX1T1 Gene Signatures in Clear Cell Renal Cell Carcinoma

  2014cited by this paper
• Privacy in the Genomic Era

  2014cited by this paper
• Revealing the results of whole-genome sequencing and whole-exome sequencing in research and clinical investigations: some ethical issues

  2014cited by this paper
• RNA sequencing identifies novel markers of non-small cell lung cancer.

  2014cited by this paper
• Privacy and protection in the genomic era

  2013cited by this paper
• Identifying Personal Genomes by Surname Inference

  2013cited by this paper
• Research ethics in the post‐genomic era

  2013cited by this paper
• Multiplex Genome Engineering Using CRISPR/Cas Systems

  2013cited by this paper
• Non-Coding RNAs: Multi-Tasking Molecules in the Cell

  2013cited by this paper
• Inactivation of Hepatitis B Virus Replication in Cultured Cells and In Vivo with Engineered Transcription Activator-Like Effector Nucleases

  2013cited by this paper
• INAUGURAL ARTICLE by a Recently Elected Academy Member:A public resource facilitating clinical use of genomes

  2012cited by this paper
• Therapy-, gender- and race-specific microRNA markers, target genes and networks related to glioblastoma recurrence and survival.

  2011cited by this paper
• MicroRNAs in Human Diseases: From Cancer to Cardiovascular Disease

  2011cited by this paper
• The DNA damage response: making it safe to play with knives.

  2010cited by this paper
• Whole exome sequencing identifies recessive WDR62 mutations in severe brain malformations

  2010cited by this paper
• Assessing Patient Readiness for the Clinical Adoption of Personalized Medicine

  2009cited by this paper
• Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of SCID-X1 patients.

  2008cited by this paper
• Mapping and quantifying mammalian transcriptomes by RNA-Seq

  2008cited by this paper
• Genome-scale DNA methylation maps of pluripotent and differentiated cells

  2008cited by this paper
• Genome-wide maps of chromatin state in pluripotent and lineage-committed cells

  2007cited by this paper
• High-resolution profiling of histone methylations in the human genome.

  2007cited by this paper
• Pharmacogenetics and geographical ancestry: implications for drug development and global health

  2005cited by this paper
• Here is the evidence, now what is the hypothesis? The complementary roles of inductive and hypothesis-driven science in the post-genomic era.

  2004cited by this paper
• Initial sequencing and analysis of the human genome

  2001cited by this paper
• Sequence the Human Genome

  1986cited by this paper

• No citing papers are available for this paper.