High-resolution, long-term characterization of bacterial motility using optical tweezers

We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of ∼100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.

• Publication year

  2009

• Venue

  Nature Methods

• Publication date

  2009-09-18

• Fields of study

  Biology, Materials Science, Physics, Engineering, Medicine

• Identifiers
  DOI 10.1038/nmeth.1380PMID 19801991PMCID 2784139
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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