Convergence of Genes and Cellular Pathways Dysregulated in Autism Spectrum Disorders

D. Pinto,Elsa Delaby,Elsa Delaby,Elsa Delaby,D. Merico,M. Barbosa,Alison K. Merikangas,L. Klei,B. Thiruvahindrapuram,Xiao Xu,R. Ziman,Zhuozhi Wang,J. Vorstman,A. Thompson,Regina Regan,Regina Regan,M. Pilorge,M. Pilorge,M. Pilorge,G. Pellecchia,A. Pagnamenta,B. Oliveira,B. Oliveira,C. Marshall,Tiago R. Magalhães,Tiago R. Magalhães,J. Lowe,J. Howe,A. Griswold,J. Gilbert,E. Duketis,B. Dombroski,M. Jonge,M. Cuccaro,Emily L. Crawford,C. Correia,C. Correia,J. Conroy,I. Conceição,I. Conceição,A. Chiocchetti,J. Casey,J. Casey,Guiqing Cai,C. Cabrol,C. Cabrol,C. Cabrol,N. Bolshakova,E. Bacchelli,R. Anney,S. Gallinger,M. Cotterchio,G. Casey,L. Zwaigenbaum,Kerstin Wittemeyer,Kirsty Wing,S. Wallace,H. Engeland,A. Tryfon,Susanne A. Thomson,L. Soorya,B. Rogé,W. Roberts,F. Poustka,S. Mouga,N. Minshew,L. A. McInnes,S. McGrew,C. Lord,M. Leboyer,A. Couteur,A. Kolevzon,P. J. González,Suma Jacob,Suma Jacob,R. Holt,Stephen J. Guter,Jonathan Green,Andrew Green,Andrew Green,C. Gillberg,B. Fernandez,F. Duque,R. Delorme,G. Dawson,Pauline Chaste,C. Café,Sean Brennan,T. Bourgeron,P. Bolton,P. Bolton,Sven Bölte,Sven Bölte,R. Bernier,G. Baird,Anthony J. Bailey,Anthony J. Bailey,E. Anagnostou,J. Almeida,E. Wijsman,V. Vieland,A. Vicente,A. Vicente,G. Schellenberg,M. Pericak-Vance,A. Paterson,J. Parr,G. Oliveira,J. Nurnberger,Anthony P. Monaco,Anthony P. Monaco,E. Maestrini,S. Klauck,H. Hakonarson,J. Haines,D. Geschwind,C. Freitag,S. Folstein,S. Ennis,S. Ennis,H. Coon,A. Battaglia,P. Szatmari,J. Sutcliffe,Joachim Hallmayer,M. Gill,E. Cook,J. Buxbaum,B. Devlin,L. Gallagher,Catalina Betancur,Catalina Betancur,Catalina Betancur,S. Scherer

Published 2014 in American Journal of Human Genetics

ABSTRACT

Rare copy-number variation (CNV) is an important source of risk for autism spectrum disorders (ASDs). We analyzed 2,446 ASD-affected families and confirmed an excess of genic deletions and duplications in affected versus control groups (1.41-fold, p = 1.0 × 10−5) and an increase in affected subjects carrying exonic pathogenic CNVs overlapping known loci associated with dominant or X-linked ASD and intellectual disability (odds ratio = 12.62, p = 2.7 × 10−15, ∼3% of ASD subjects). Pathogenic CNVs, often showing variable expressivity, included rare de novo and inherited events at 36 loci, implicating ASD-associated genes (CHD2, HDAC4, and GDI1) previously linked to other neurodevelopmental disorders, as well as other genes such as SETD5, MIR137, and HDAC9. Consistent with hypothesized gender-specific modulators, females with ASD were more likely to have highly penetrant CNVs (p = 0.017) and were also overrepresented among subjects with fragile X syndrome protein targets (p = 0.02). Genes affected by de novo CNVs and/or loss-of-function single-nucleotide variants converged on networks related to neuronal signaling and development, synapse function, and chromatin regulation.

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