Transient N-acetylglucosamine in the biosynthesis of phytohemagglutinin: attachment in the Golgi apparatus and removal in protein bodies

Cotyledons of the common bean (Phaseolus vulgaris L.) synthesize large amounts of the lectin phytohemagglutinin (PHA) during seed development. The polypeptides of PHA are synthesized by endoplasmic reticulum-bound polysomes and co-translationally glycosylated, pass through the Golgi complex, and accumulate in protein bodies, which constitute the lysosomal compartment in these cells. Some of the high-mannose sidechains of PHA are modified in the Golgi complex, and in mature PHA they contain N-acetylglucosamine, mannose, fucose, and xylose in the molar ratios 2, 3.8, 0.6, and 0.5. The results reported here show that the Golgi complex is also the site of additional N-acetylglucosamine incorporation into the modified sidechains. When developing cotyledons are labeled with [3H]glucosamine and glycopeptides of PHA present in the Golgi complex isolated, the radioactivity can be released as [3H]N- acetylglucosamine by digestion of the glycopeptides with beta-N- acetylglucosaminidase, indicating that the residues are in a terminal position. Arrival of PHA in the protein bodies is followed by the slow removal of these terminal N-acetylglucosamine residues, resulting in a decrease in the Mr of the modified sidechains. The biosynthetic intermediates of the glycoproteins destined for the lysosomal compartments of animal cells contain high-mannose sidechains modified by phosphate groups covered by N-acetylglucosamine that is labile to mild acid treatment. When cotyledons are labeled with [32P]orthophosphate, there is no radioactivity in PHA obtained from any of the subcellular fractions. There is also no release of radioactivity when [3H]glucosamine-labeled glycopeptides obtained from PHA in the Golgi complex are subjected to mild acid hydrolysis. These results indicate that the sorting-signals and posttranslational processing steps for proteins that are transported to the lysosomal compartment are different in plant cells and animal cells.

• Publication year

  1984

• Venue

  Journal of Cell Biology

• Publication date

  1984-07-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1083/JCB.99.1.133PMID 6429153PMCID 2275646
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Characterization and subcellular localization of vicilin and phytohemagglutinin, the two major reserve proteins of Phaseolus vulgaris L.

  2004cited by this paper
• Biosynthesis and processing of phytohemagglutinin in developing bean cotyledons.

  1984cited by this paper
• The Golgi apparatus mediates the transport of phytohemagglutinin to the protein bodies in bean cotyledons

  1983cited by this paper
• Incorporation of fucose into the carbohydrate moiety of phytohemagglutinin in developing Phaseolus vulgaris cotyledons

  1983cited by this paper
• Interaction of concanavalin A with native and denatured forms of jackbean alpha-D-mannosidase.

  1983cited by this paper
• Immunocytochemical localisation of lectins in cells of Phaseolus vulgaris L. seeds

  1982cited by this paper
• Biosynthesis of lysosomal hydrolases: their synthesis in bound polysomes and the role of co- and post-translational processing in determining their subcellular distribution

  1982cited by this paper
• The immuno-histochemical localization of lectin in pea seeds (Pisum sativum L.)

  1981cited by this paper
• Subcellular location of two enzymes involved in the synthesis of phosphorylated recognition markers in lysosomal enzymes.

  1981cited by this paper
• Two Kinds of Protein Glycosylation in a Cell-Free Preparation from Developing Cotyledons of Phaseolus vulgaris.

  1981cited by this paper
• The role of glycosylation in storage-protein synthesis in developing pea seeds

  1981cited by this paper
• Uptake and apparent digestion of cytoplasmic organelles by protein bodies (protein storage vacuoles) in mung bean cotyledons.

  1981cited by this paper
• Pulse-labeling Studies on Protein Synthesis in Developing Pea Seeds and Evidence of a Precursor Form of Legumin Small Subunit.

  1980cited by this paper
• Phosphorylated oligosaccharides in lysosomal enzymes: identification of alpha-N-acetylglucosamine(1)phospho(6)mannose diester groups.

  1980cited by this paper
• The Endoplasmic Reticulum of Mung Bean Cotyledons: ROLE IN THE ACCUMULATION OF HYDROLASES IN PROTEIN BODIES DURING SEEDLING GROWTH.

  1980cited by this paper
• Protein bodies of mung bean cotyledons as autophagic organelles.

  1980cited by this paper
• Ultrastructural localization of soybean agglutinin on thin sections of Glycine max (soybean) var. Altona by the gold method

  1980cited by this paper
• Biosynthetic intermediates of beta-glucuronidase contain high mannose oligosaccharides with blocked phosphate residues.

  1980cited by this paper
• Biosynthesis of lysosomal enzymes in fibroblasts. Phosphorylation of mannose residues.

  1980cited by this paper
• OLIGOSACCHARIDE PROCESSING DURING GLYCOPROTEIN BIOSYNTHESIS

  1979cited by this paper
• Glycoprotein Synthesis in Plants: III. Interaction between UDP-N-Acetylglucosamine and GDP-Mannose as Substrates.

  1978cited by this paper
• Localization of vicilin peptidohydrolase in the cotyledons of mung bean seedlings by immunofluorescence microscopy

  1978cited by this paper
• Purification of the phytohemagglutinin family of proteins from red kidney beans (Phaseolus vulgaris) by affinity chromatography.

  1975cited by this paper
• Growth of Immature Pea Cotyledons in Culture

  1975cited by this paper
• Histochemical and biochemical observations on storage protein metabolism and protein body autolysis in cotyledons of germinating mung beans.

  1975cited by this paper
• Intracellular Distribution of Proteins in Pea Cotyledons.

  1963cited by this paper
• Intracellular Distribution of Proteins in Pea Cotyledons"-2

  year unknowncited by this paper

• Members of the microalgae family Scenedesmaceae feature individual N-glycan patterns, a shared Man5GlcNAc2 isomer and core alpha1,6-fucosylation

  2025cites this paper
• Isolation of Momordica charantia seed lectin and glycosidases from the protein bodies: Lectin-glycosidase (β-hexosaminidase) protein body membrane interaction reveals possible physiological function of the lectin.

  2023cites this paper
• Posttranslational Modification of Heterologous Human Therapeutics in Plant Host Expression Systems

  2019cites this paper
• Advances in the Biology of Seed and Vegetative Storage Proteins Based on Two-Dimensional Electrophoresis Coupled to Mass Spectrometry

  2018cites this paper
• Glyco-Engineering of Plant-Based Expression Systems.

  2018cites this paper
• Production of Functionally Active Recombinant Proteins in Plants

  2018cites this paper
• Perspectives for the Algae-Made Biopharmaceuticals Field

  2016cites this paper
• Plant protein glycosylation

  2016cites this paper
• The Arabidopsis tonoplast is almost devoid of glycoproteins with complex N-glycans, unlike the rat lysosomal membrane

  2016cites this paper
• The production of human glucocerebrosidase in glyco‐engineered Nicotiana benthamiana plants

  2016cites this paper
• Structural features of N-glycans linked to glycoproteins expressed in three kinds of water plants: Predominant occurrence of the plant complex type N-glycans bearing Lewis a epitope.

  2016cites this paper
• Algae-Based Biopharmaceuticals

  2016cites this paper
• Diatom-Specific Oligosaccharide and Polysaccharide Structures Help to Unravel Biosynthetic Capabilities in Diatoms

  2015cites this paper
• Enzymatic properties and subtle differences in the substrate speci fi city of phylogenetically distinct invertebrate N-glycan processing

  2015cites this paper
• Enzymatic properties and subtle differences in the substrate specificity of phylogenetically distinct invertebrate N-glycan processing hexosaminidases

  2014cites this paper
• Structural features of free N-glycans occurring in plants and functional features of de-N-glycosylation enzymes, ENGase, and PNGase: the presence of unusual plant complex type N-glycans

  2014cites this paper
• Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals

  2014cites this paper
• The potential of Physcomitrella patens as a platform for the production of plant-based vaccines

  2014cites this paper
• -N-Acetylhexosaminidase involvement in -conglutin mobilization in

  2013cites this paper
• β-N-Acetylhexosaminidase involvement in α-conglutin mobilization in Lupinus albus.

  2013cites this paper
• Biochemical Characterization of a β-N-acetylhexosaminidase from Fig Latex

  2011cites this paper
• N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells.

  2011cites this paper
• N-Glycans of Phaeodactylum tricornutum Diatom and Functional Characterization of Its N-Acetylglucosaminyltransferase I Enzyme*

  2010cites this paper
• Plant glycans: friend or foe in vaccine development?

  2010cites this paper
• Mgat1-dependent N-glycans are essential for the normal development of both vertebrate and invertebrate metazoans.

  2010cites this paper
• Recombinant human GAD65 accumulates to high levels in transgenic tobacco plants when expressed as an enzymatically inactive mutant.

  2010cites this paper
• Plant glycosidases acting on protein-linked oligosaccharides.

  2009cites this paper
• The functions of paucimannose N-glycans in Caenorhabditis elegans

  2009cites this paper
• 線虫におけるパウチマンノース型 N-グリカンの機能

  2009cites this paper
• Localization of plant N‐glycan processing enzymes along the secretory pathway

  2009cites this paper
• N-glycosylation in plants: science and application

  2009cites this paper
• Paucimannose N-glycans in Caenorhabditis elegans and Drosophila melanogaster.

  2009cites this paper
• Glyco-engineering of biotherapeutic proteins in plants.

  2008cites this paper
• Identification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan Processing[W]

  2008cites this paper
• Biosynthesis and Degradation of Mono-, Oligo-, and Polysaccharides: Introduction

  2008cites this paper
• Molecular Basis for the Biosynthesis of Oligo- and Polysaccharides

  2008cites this paper
• Enzymatic Properties and Subcellular Localization of Arabidopsis β-N-Acetylhexosaminidases1[W][OA]

  2007cites this paper
• A Unique β1,3-Galactosyltransferase Is Indispensable for the Biosynthesis of N-Glycans Containing Lewis a Structures in Arabidopsis thaliana[W][OA]

  2007cites this paper
• A Unique b 1,3-Galactosyltransferase Is Indispensable for the Biosynthesis of N -Glycans Containing Lewis a Structures in Arabidopsis thaliana

  2007cites this paper
• Biosynthesis of Truncated N-Linked Oligosaccharides Results from Non-orthologous Hexosaminidase-mediated Mechanisms in Nematodes, Plants, and Insects*

  2007cites this paper
• 4.06 – Glycobiology of Caenorhabditis elegans

  2007cites this paper
• 3.02 – Processing of Protein-Bound N-Glycans

  2007cites this paper
• Protein N-glycosylation in the baculovirus-insect cell system.

  2007cites this paper
• The Drosophila fused lobes Gene Encodes an N-Acetylglucosaminidase Involved in N-Glycan Processing*

  2006cites this paper
• N-glycans are involved in the response of Caenorhabditis elegans to bacterial pathogens.

  2006cites this paper
• Null Mutations in Drosophila N-Acetylglucosaminyltransferase I Produce Defects in Locomotion and a Reduced Life Span*

  2006cites this paper
• Molecular cloning and functional characterization of beta-N-acetylglucosaminidase genes from Sf9 cells.

  2006cites this paper
• Modification of plant N‐glycans processing: The future of producing therapeutic protein by transgenic plants

  2005cites this paper
• The In Vivo Synthesis and Accumulation of Lectin in Developing Seeds of Black Gram (Vigna mungo L. Hepper)

  2004cites this paper
• Protein glycosylation lessons from Caenorhabditis elegans.

  2004cites this paper
• Purification and characterization of isoforms of β-N- acetylhexosaminidase from mungbean seedlings

  2004cites this paper
• Purification and characterization of β-N-acetylhexosaminidase from maize seedlings

  2003cites this paper
• Synthesis of paucimannose N-glycans by Caenorhabditis elegans requires prior actions of UDP-N-acetyl-D-glucosamine:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, alpha3,6-mannosidase II and a specific membrane-bound beta-N-acetylglucosaminidase.

  2003cites this paper
• Purification and characterization of beta-N-acetylhexosaminidase from maize seedlings.

  2003cites this paper
• UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis elegans.

  2002cites this paper
• UDP-N-acetylglucosamine:α-3-d-mannoside β-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine:α-6-d-mannoside β-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis elegans

  2002cites this paper
• Control of the N-Glycosylation of Therapeutic Glycoproteins Produced in Transgenic Plants: A New Challenge for Glycobiologists

  2002cites this paper
• Functional post-translational proteomics approach to study the role of N-glycans in the development of Caenorhabditis elegans.

  2002cites this paper
• Purification and characterization of beta-N-acetylhexosaminidase from rice seeds.

  2002cites this paper
• Purification and Characterization of β-N-Acetylhexosaminidase from Rice Seeds

  2002cites this paper
• Small complex-type N-linked glycans are attached to cell-wall bound exo-beta-glucanases of both mung bean and barley seedlings.

  2001cites this paper
• Influence of growth conditions and developmental stage on N-glycan heterogeneity of transgenic immunoglobulin G and endogenous proteins in tobacco leaves.

  2001cites this paper
• β(1,2)-Xylose and α(1,3)-Fucose Residues Have a Strong Contribution in IgE Binding to Plant Glycoallergens*

  2000cites this paper
• MINI REVIEW Function and structure of Drosophila glycans

  2000cites this paper
• Function and structure of Drosophila glycans.

  2000cites this paper
• Characterization of N-glycans from Arabidopsis. Application to a fucose-deficient mutant.

  1999cites this paper
• Fucose in N-glycans: from plant to man.

  1999cites this paper
• Protein Storage Bodies and Vacuoles

  1999cites this paper
• Biosynthesis and immunolocalization of Lewis a-containing N-glycans in the plant cell.

  1999cites this paper
• Phaseolin: its Past, Properties, Regulation and Future

  1999cites this paper
• Analysis of N - and O -Glycosylation of Plant Proteins

  1998cites this paper
• Deposition of storage proteins

  1998cites this paper
• The protein N-glycosylation in plants

  1998cites this paper
• Protein Trafficking in Plant Cells

  1998cites this paper
• Sorting of proteins to vacuoles in plant cells

  1998cites this paper
• N-Glycoprotein biosynthesis in plants: recent developments and future trends

  1998influential citation
• Compartmentation of plant cell proteins in separate lytic and protein storage vacuoles

  1998cites this paper
• Isolation and characterization of a class II alpha-mannosidase cDNA from lepidopteran insect cells.

  1997cites this paper
• Protein quality control along the route to the plant vacuole.

  1997cites this paper
• Protein targeting to the plant vacuole--a historical perspective.

  1996cites this paper
• Mechanisms of Intracellular Protein Transport and Targeting in Plant Cells

  1996cites this paper
• Insertion intoAspergillus nidulans of functional UDP-GlcNAc: α3-d-mannoside β-1,2-N-acetylglucosaminyltransferase I, the enzyme catalysing the first committed step from oligomannose to hybrid and complex N-glycans

  1995cites this paper
• Chapter 10 Protein Glycosylation in Insects

  1995cites this paper
• Chapter 9 N-Glycosylation of Plant Proteins

  1995cites this paper
• Insect Cells Contain an Unusual, Membrane-bound -N-Acetylglucosaminidase Probably Involved in the Processing of Protein N-Glycans (*)

  1995cites this paper
• Structures of the N-linked oligosaccharides of the membrane glycoproteins from three lepidopteran cell lines (Sf-21, IZD-Mb-0503, Bm-N).

  1994cites this paper
• Processing of asparagine-linked oligosaccharides in insect cells. N-acetylglucosaminyltransferase I and II activities in cultured lepidopteran cells.

  1993cites this paper
• Zinc-Finger Proteins in Oncogenesis: DNA-Binding and Gene Regulation. Conference proceedings. Noordwijkerhout, The Netherlands, September 6-9, 1992.

  1993cites this paper
• Studies on synthetic pathway of xylose-containing N-linked oligosaccharides deduced from substrate specificities of the processing enzymes in sycamore cells (Acer pseudoplatanus L.).

  1992cites this paper
• Biosynthesis, processing and targeting of the G-protein of vesicular stomatitis virus in tobacco protoplasts

  1992cites this paper
• Isolation and partial characterization of binding proteins for immobilized delta endotoxin from solubilized brush border membrane vesicles of the silkworm, Bombyx mori, and the common cutworm, Spodoptera litura.

  1992cites this paper
• The α-amylase inhibitor of bean seed: two-step proteolytic maturation in the protein storage vacuoles of the developing cotyledon

  1992cites this paper
• Sorting of proteins to the vacuoles of plant cells

  1992cites this paper
• 1H-NMR structural determination of the N-linked carbohydrate chains on glycopeptides obtained from the bean lectin phytohemagglutinin.

  1992cites this paper
• Characterisation of oligosaccharides from Drosophila melanogaster glycoproteins.

  1991cites this paper
• Primary structures of N-linked oligosaccharides of momordin-a, a ribosome-inactivating protein from Momordica charantia seeds.

  1991cites this paper
• LECTIN AND RELATED PROTEINS IN THE BARK OF Sophora japonica L

  1991cites this paper
• Protein transport and targeting within the endomembrane system of plants

  1991cites this paper
• Heterogeneity of the complex N-linked oligosaccharides at specific glycosylation sites of two secreted carrot glycoproteins.

  1991cites this paper
• Structure and biosynthesis of the xylose‐containing carbohydrate moiety of rice α‐amylase

  1990cites this paper