Feedback suppression of the immune response in vitro. II. IgVH- restricted antibody-dependent suppression

Feedback suppression of the primary humoral immune response to sheep erythrocytes (SRBC) in vitro was induced with cell-free supernate material derived from antigen-(SRBC) activated B (sIg+) cells. This soluble products bears Ig determinants and binds to the eliciting antigen (SRBC). The activity of this antibody in suppressing anti-SRBC plaque-forming cell responses is restricted to spleen cell cultures containing B cells sharing VH genes with the B cells producing the suppressive antibody. The anti-hapten (trinitrophenyl) response to derivatized SRBC is not affected by antigen-primed B cells or their products. These data are compatible with suppression being mediated by anti-antigen antibody, either (a) via blockade of different SRBC epitopes recognized by a limited set of B cell clones in each mouse strain, (b) via triggering of an anti-idiotypic response, either antibody or suppressor T cell in nature, restricted to activity in cultures containing B cells sharing VH structures with the original antibody, or (c) via interference by preformed antibody with T cell help directed at idiotype bearing B cells.

• Publication year

  1980

• Venue

  Journal of Experimental Medicine

• Publication date

  1980-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.151.3.681PMID 6444664PMCID 2185808
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The anti-hapten response to derivatized SRBC is not affected, and the findings are compatible with suppression by anti-antigen antibody through epitope blockade, anti-idiotypic effects, or interference with T cell help to idiotype-bearing B cells.

  Confidence 0.87

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• The suppressive activity carries Ig determinants, binds the eliciting SRBC antigen, and is restricted to cultures containing B cells that share VH genes with the B cells producing the suppressive antibody.

  Confidence 0.94

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• Cell-free supernate from antigen-activated B cells suppresses the primary anti-SRBC plaque-forming cell response in vitro.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review

• antigen-activated b cells

  cell populations

  B cells that have been stimulated by SRBC antigen and are the source of the soluble suppressive material.

  Aliases: SRBC-activated B cells, activated B cells

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• anti-hapten response

  immune responses

  The antibody response directed against the hapten coupled to SRBC rather than against SRBC itself.

  Aliases: anti-trinitrophenyl response, anti-TNP response

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• anti-idiotypic response

  immune responses

  An immune response directed against idiotypic determinants of an antibody or B-cell receptor.

  Aliases: anti-idiotype response, anti-idiotypic effects

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• anti-srbc plaque-forming cell response

  immune responses

  The in vitro antibody-secreting cell response directed against sheep erythrocytes and measured by plaque formation.

  Aliases: anti-sheep erythrocyte PFC response, anti-SRBC PFC response

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• cell-free supernate material

  materials

  Soluble material collected from B-cell culture supernatants and used as the suppressive preparation in vitro.

  Aliases: supernate, cell-free supernate

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• derivatized srbc

  antigens

  SRBC modified by attachment of a hapten, creating the antigen used for the anti-hapten assay.

  Aliases: haptenated SRBC, trinitrophenylated SRBC

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• idiotype-bearing b cells

  cell populations

  B cells expressing antibody variable-region idiotypes on their surface immunoglobulin receptors.

  Aliases: idiotypic B cells

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• ig determinants

  molecular features

  Immunoglobulin-associated antigenic determinants present on the suppressive soluble product.

  Aliases: immunoglobulin determinants, Ig epitopes

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• sheep erythrocytes (srbc)

  antigens

  Sheep red blood cells used as the antigenic target in the immune-response assays.

  Aliases: SRBC, sheep red blood cells

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• suppressive antibody

  molecules

  Antibody produced by antigen-activated B cells that mediates the observed suppression in culture.

  Aliases: suppression antibody, antibody-dependent suppressor

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• t cell help

  cellular interactions

  Helper T-cell support that promotes B-cell activation and antibody production.

  Aliases: T-cell help, helper T-cell support

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• vh gene sharing

  genetic features

  Overlap in immunoglobulin heavy-chain variable-region gene usage between different B-cell populations.

  Aliases: shared VH genes, VH-restricted

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review

• Feedback suppression of the immune response in vitro. I. Activity of antigen-stimulated B cells

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