Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis

No abstract is available for this paper.

• Publication year

  2004

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2004-02-09

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1073/pnas.0305476101PMID 14769930PMCID PMC357030
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• PCR 2 : a practical approach

  2016cited by this paper
• Biotechnology에서 배우는 교훈

  2006cited by this paper
• The Metabolic and Molecular Basis of Inherited Disease: Protein Folding and Misfolding: the Role of Cellular Protein Quality Control Systems in Inherited Disorders

  2005cited by this paper
• Genotyping SNPs with molecular beacons.

  2003cited by this paper
• Real-time PCR in virology.

  2002cited by this paper
• Real‐time PCR with molecular beacons provides a highly accurate assay for detection of Tay‐Sachs alleles in single cells

  2002cited by this paper
• QuantiLyse: reliable DNA amplification from single cells.

  2002cited by this paper
• Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence.

  2002cited by this paper
• Naturally occurring mutations in GM2 gangliosidosis: a compendium.

  2001cited by this paper
• Molecular epidemiology of Tay-Sachs disease.

  2001cited by this paper
• MELTING, computing the melting temperature of nucleic acid duplex

  2001cited by this paper
• Symmetric vs asymmetric PCR and molecular beacon probe in the detection of a target gene of adenovirus.

  2000cited by this paper
• Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres.

  2000cited by this paper
• Current applications of single-cell PCR

  2000cited by this paper
• Digital PCR.

  1999cited by this paper
• Multicolor molecular beacons for allele discrimination

  1998cited by this paper
• A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics.

  1998cited by this paper
• Thermodynamics and NMR of internal G.T mismatches in DNA.

  1997cited by this paper
• Real time quantitative PCR.

  1996cited by this paper
• Molecular Beacons: Probes that Fluoresce upon Hybridization

  1996cited by this paper
• PCR 2. A practical approach: Edited by M.J. McPherson, B.D. Hames and G.R. Taylor, Oxford University Press; Oxford, 1995. xxv + 332 pp. £25.00 (pb). ISBN 0-19-963424-6

  1996cited by this paper
• Sequencing of in vitro amplified DNA.

  1993cited by this paper
• Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

  1993cited by this paper
• Production of single-stranded DNA for sequencing: an alternative approach.

  1992cited by this paper
• PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA.

  1991cited by this paper
• Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.

  1989cited by this paper
• Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction.

  1989cited by this paper
• Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

  1988cited by this paper
• Amplification and analysis of DNA sequences in single human sperm and diploid cells

  1988cited by this paper
• Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A, C, G, T)

  1985cited by this paper
• Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

  1979cited by this paper
• A rapid method for detecting and mapping homology between heterologous DNAs. Evaluation of polyomavirus genomes.

  1979cited by this paper

• Multiscale microfluidic platform with vacuum-driven chambers for automated high-volume ssDNA generation.

  2025cites this paper
• Fast DNA‐Based Molecular Classifier for Cancer Diagnosis Using Freeze‐Thaw Cycling†

  2025cites this paper
• Non-saturated nucleic acid probes with a broad dynamic range.

  2025cites this paper
• A Multi‐Input Molecular Classifier Based on Digital DNA Strand Displacement for Disease Diagnostics

  2025cites this paper
• qPCR-melting curve analysis in molecular diagnostics.

  2025cites this paper
• Development of PCR-based test systems for genotyping of single nucleotide polymorphisms in innate immunity and inflammatory response genes

  2025cites this paper
• Development and optimization of an antibody-free nucleic acid lateral flow assay (AF-NALFA) as part of a molecular toolkit for visual readout of amplified Listeria monocytogenes DNA.

  2025cites this paper
• Coupling Exponential to Linear Amplification for Endpoint Quantitative Analysis

  2024cites this paper
• Evaluation of the FluoroType Mycobacteria assay for identification of mycobacteria

  2024cites this paper
• DNA-based molecular classifiers for the profiling of gene expression signatures

  2024cites this paper
• Recent Advances in Closed-Tube Barcoding for FastFish-ID.

  2024cites this paper
• Dual-centre evaluation of the FluoroType MTBDR version 2 assay for detection of Mycobacterium tuberculosis complex and resistance-conferring mutations in pulmonary and extrapulmonary samples from Denmark, Germany and Sierra Leone.

  2024cites this paper
• Techniques, procedures, and applications in host genetic analysis.

  2024cites this paper
• NASBA Coupled to Paper Microfluidics for RNA Detection.

  2024cites this paper
• Expanding Cas12a Activity Control with an RNA G‐Quadruplex at the 5′ end of CRISPR RNA

  2024cites this paper
• A Cost‐Effective Approach for Single‐Stranded DNA Amplification Using Primer‐Blocked Asymmetric PCR

  2024cites this paper
• Multiplex Asymmetric PCR by Combining the Amplification Refractory Mutation System with the Homo-Tag-Assisted Nondimer System.

  2024cites this paper
• Detection of Mtb and NTM: preclinical validation of a new asymmetric PCR-binary deoxyribozyme sensor assay

  2024cites this paper
• Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations.

  2023cites this paper
• Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health

  2023cites this paper
• Host-associated microbe PCR (hamPCR): accessing new biology through convenient measurement of both microbial load and community composition

  2023cites this paper
• Site-specific insertion of endonuclease recognition sites into amplicons to improve post-PCR analysis sensitivity of gene mutation.

  2022cites this paper
• Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots

  2022cites this paper
• SAMBA: A Multicolor Digital Melting PCR Platform for Rapid Microbiome Profiling

  2022cites this paper
• PAM-independent ultra-specific activation of CRISPR-Cas12a via sticky-end dsDNA

  2022cites this paper
• Lambda‐PCR for precise DNA assembly and modification

  2022cites this paper
• Robust and scalable barcoding for massively parallel long-read sequencing

  2022cites this paper
• A Comparison of Methods for the Production of Kilobase-Length Single-Stranded DNA

  2022cites this paper
• Point of care diagnostics and non-invasive sampling strategy: a review on major advances in veterinary diagnostics

  2022cites this paper
• A Biochip for Genotyping Polymorphisms Associated with Eye, Hair, Skin Color, AB0 Blood Group, Sex, Y Chromosome Core Haplogroup, and Its Application to Study the Slavic Population

  2022influential citation
• Specific and robust hybridization based on double-stranded nucleic acids with single-base resolution.

  2022cites this paper
• RT-qPCR Diagnostics: The “Drosten” SARS-CoV-2 Assay Paradigm

  2021cites this paper
• Validation of FASTFISH-ID: A new commercial platform for rapid fish species authentication via universal closed-tube barcoding.

  2021cites this paper
• Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern uganda by high‐resolution melt analysis

  2021cites this paper
• Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage

  2021cites this paper
• A novel RNA detection technique for point-of-care identification of pathogens

  2021cites this paper
• Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition

  2021cites this paper
• Highly specific detection of KRAS single nucleotide polymorphism by asymmetric PCR/SERS assay.

  2021cites this paper
• Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting

  2021cites this paper
• Effect of TRPM8 and TRPA1 Polymorphisms on COPD Predisposition and Lung Function in COPD Patients

  2021cites this paper
• A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs

  2020cites this paper
• Rapid and simple detection of isoniazid resistant Mycobacterium tuberculosis utilizing DNA chromatography based technique.

  2020cites this paper
• DNA detection by SERS: hybridisation parameters and the potential for asymmetric PCR.

  2020cites this paper
• One-Pot Synthesis of Defined-Length ssDNA for Multiscaffold DNA Origami

  2020cites this paper
• Rapid Pyrazinamide Drug Susceptibility Testing using a Closed-Tube PCR Assay of the Entire pncA gene

  2020cites this paper
• Fusion Strategy of Influenza A-H5N1 Virus M2e Epitope DNA Sequence to Hepatitis B Virus HBsAg-S Gene

  2020cites this paper
• Measuring both microbial load and diversity with a single amplicon sequencing library

  2020cites this paper
• Novel Strategies to Optimize the Amplification of Single-Stranded DNA

  2020cites this paper
• Progress of Aptamer Screening Techniques Based on Microfluidic Chips

  2020cites this paper
• Rapidemic, a versatile and label-free DNAzyme-based platform for visual nucleic acid detection

  2020cites this paper
• A simple modification increases specificity and efficiency of asymmetric PCR.

  2019cites this paper
• ROLE OF GABAERGIC SYSTEM GENETIC POLYMORPHISMS IN THE DEVELOPMENT OF OBSTRUCTIVE SLEEP APNEA SYNDROME IN ASTHMA PATIENTS

  2019cites this paper
• Development and evaluation of a pan‐dermatophyte polymerase chain reaction with species‐level identification using sloppy molecular beacon probes

  2019cites this paper
• Development of HyBeacon® probes for the forensic detection of Panthera, rhinoceros, and pangolin species.

  2019cites this paper
• Epidemiologia de les rickettsiosis del grup de les febres tacades a Catalunya. Estudi específic de la Rickettsia massiliae Bar 29. Eficàcia de les tècniques diagnòstiques

  2019cites this paper
• Structural variability of DNA-containing Mg-pyrophosphate microparticles: optimized conditions to produce particles with desired size and morphology

  2019cites this paper
• Variety of bio-nanogold in the fabrication of lateral flow biosensors for detection of pathogenic bacteria.

  2019cites this paper
• Advances in the molecular diagnosis of tuberculosis: From probes to genomes.

  2019cites this paper
• Multiplex Lateral Flow Assay for Rapid Visual Blood Group Genotyping.

  2018cites this paper
• Validation of the FluoroType MTBDR Assay for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Complex Isolates

  2018cites this paper
• Validation of the FluoroType® MTBDR assay for the detection of rifampicin and 1 isoniazid resistance in Mycobacterium tuberculosis complex isolates

  2018cites this paper
• Combining asymmetric PCR-based enzymatic amplification with silicon photonic microring resonators for the detection of lncRNAs from low input human RNA samples.

  2018cites this paper
• Validation of the FluoroType® MTBDR assay for the detection of rifampicin and 1 isoniazid resistance in Mycobacterium tuberculosis complex isolates

  2018cites this paper
• Multi-Target Strategy for Pan/Foot-and-Mouth Disease Virus (FMDV) Detection: A Combination of Sequences Analysis, in Silico Predictions and Laboratory Diagnostic Evaluation

  2018cites this paper
• Vastagbélrák-hajlamosító DNS-szekvencia-variációk tumormentes és vastagbél-daganatos populációban Magyarországon

  2018cites this paper
• Denaturation-Enhanced Droplet Digital PCR for Liquid Biopsies.

  2018cites this paper
• In vitro synthesis of gene-length single-stranded DNA

  2018cites this paper
• An array-based melt curve analysis method for the identification and classification of closely related pathogen strains

  2018cites this paper
• Detection of multiple organisms based on the distance-dependent optical properties of gold nanoparticle and dark-field microscopy.

  2018cites this paper
• CHEM-NAT: a unique chemical approach for nucleic acids testing

  2018cites this paper
• Validation of the FluoroType® MTBDR assay using respiratory and lymph node samples.

  2018cites this paper
• Chapter 2 A Simplifi ed Protocol for Preparing Pyrosequencing Templates Based on LATE-PCR Using Whole Blood as Starting Material Directly

  2018cites this paper
• A specific but nevertheless simple real-time PCR method for the detection of irradiated food shown detailed at the example of garlic (Allium sativum)

  2018cites this paper
• [Colorectal cancer susceptibility genetic variants in tumor free and colorectal carcinoma bearing Hungarian population. Individual predisposition to cancer].

  2018cites this paper
• Novel Molecular Diagnostics and Therapeutic Tools for Livestock Diseases

  2018cites this paper
• Multiplexed microRNA Expression Profiling by Combined Asymmetric PCR and Label-Free Detection using Silicon Photonic Sensor Arrays.

  2018cites this paper
• Multiplexed identification, quantification and genotyping of infectious agents using a semiconductor biochip

  2018cites this paper
• Diagnostic Accuracy and Utility of FluoroType MTBDR, a New Molecular Assay for Multidrug-Resistant Tuberculosis

  2018cites this paper
• Defining And Exploiting The Cytosine Deaminase Activity Of Apobec3a On The Extended Epigenome

  2018influential citation
• Validation of the FluoroType® MTBDR assay for the detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis complex isolates

  2018cites this paper
• Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures

  2017cites this paper
• A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.

  2017cites this paper
• Digitally encoded silica microparticles for multiplexed nucleic acid detection.

  2017cites this paper
• DNA Encoded Libraries (DEGL) of Glycan Antigens to Detect Antibodies: An Approach Towards Next Generation Functional Glycomics

  2017cites this paper
• TRPM8 GENE POLYMORPHISM AND SMOKING AS THE FACTORS OF SEVERE BRONCHIAL OBSTRUCTION IN PATIENTS WITH ASTHMA

  2017influential citation
• ROLE OF TRPM8 GENE POLYMORPHISMS IN THE FORMATION OF ASTHMA PHENOTYPE WITH COLD AIRWAY HYPERRESPONSIVENESS

  2017cites this paper
• Peptide nucleic acid probe-based fluorescence melting curve analysis for rapid screening of common JAK2, MPL, and CALR mutations.

  2017cites this paper
• Germline Variation in HSD 3 B 1 as a Novel Biomarker in Prostate Cancer

  2017influential citation
• A digital microfluidic system for loop-mediated isothermal amplification and sequence specific pathogen detection

  2017cites this paper
• Closed-Tube Barcoding of Sequence Variants ( Possible Species ) within the Genus Naegleria

  2017cites this paper
• qPCR primer design revisited

  2017cites this paper
• Specificity of SNP detection with molecular beacons is improved by stem and loop separation with spacers.

  2017cites this paper
• State of diagnosing infectious pathogens using colloidal nanomaterials

  2017cites this paper
• APOBEC3A efficiently deaminates methylated, but not TET-oxidized, cytosine bases in DNA

  2017influential citation
• A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses

  2017cites this paper
• Using Polymerase Preference Index to Design imLATE-PCR Primers for an Efficient Pyrosequencing

  2016cites this paper
• A Simplified Protocol for Preparing Pyrosequencing Templates Based on LATE-PCR Using Whole Blood as Starting Material Directly

  2016cites this paper
• SELECTION OF DNA APTAMERS TO HUMAN RED BLOOD CELLS FOR DRUG DELIVERY

  2016cites this paper
• Virtual Barcoding using LATE-PCR and Lights-On/Lights-Off probes: identification of nematode species in a closed-tube reaction

  2016cites this paper
• Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes

  2016cites this paper