Capturing RNA-dependent pathways for cryo-EM analysis

Cryo-Electron Microscopy (EM) is a powerful technique to visualize biological processes at nanometer resolution. Structural studies of macromolecular assemblies are typically performed on individual complexes that are biochemically isolated from their cellular context. Here we present a molecular imaging platform to capture and view multiple components of cellular pathways within a functionally relevant framework. We utilized the bacterial protein synthesis machinery as a model system to develop our approach. By using modified Affinity Grid surfaces, we were able to recruit multiple protein assemblies bound to nascent strands of mRNA. The combined use of Affinity Capture technology and single particle electron microscopy provide the basis for visualizing RNA-dependent pathways in a remarkable new way.

• Publication year

  2012

• Venue

  Computational and Structural Biotechnology Journal

• Publication date

  2012-02-23

• Fields of study

  Biology, Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.5936/csbj.201204003PMID 24688633PMCID 3962177
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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