Reporter gene vectors and assays

No abstract is available for this paper.

• Publication year

  1999

• Venue

  Molecular Biotechnology

• Publication date

  1999-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1385/MB:13:1:29PMID 10934520
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• green fluorescent protein

  2020cited by this paper
• The β-Gal Interferon Assay: A New, Precise, and Sensitive Method

  1998cited by this paper
• Influence of the DNA complexation medium on the transfection efficiency of lipospermine/DNA particles

  1998cited by this paper
• The green fluorescent protein.

  1998cited by this paper
• Transgenic animals with inducible, targeted gene expression in brain.

  1998cited by this paper
• Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.

  1998cited by this paper
• High sensitivity for color mutants in lacZ plasmid-based transgenic mice, as detected by positive selection.

  1998cited by this paper
• GFP illuminates the cytoskeleton.

  1998cited by this paper
• A genome-wide functional assay of signal transduction in living mammalian cells

  1998cited by this paper
• Colon Epithelium Can Be Transiently Transfected with Liposomes, Calcium Phosphate Precipitation and DEAE Dextran in vivo

  1998cited by this paper
• Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter.

  1998influential reference
• A novel technique for in-vivo assay of viral regulatory regions in genomes of animal RNA viruses.

  1998cited by this paper
• Characterization of lipid DNA interactions. I. Destabilization of bound lipids and DNA dissociation.

  1998cited by this paper
• Green fluorescent protein as a signal for protein–protein interactions

  1997cited by this paper
• Use of alkaline phosphatase as a reporter polypeptide to study the role of the subtilin leader segment and the SpaT transporter in the posttranslational modifications and secretion of subtilin in Bacillus subtilis 168

  1997cited by this paper
• The development of beta-lactamase as a highly versatile genetic reporter for eukaryotic cells.

  1997cited by this paper
• Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy.

  1997cited by this paper
• Selective production of transgenic mice using green fluorescent protein as a marker

  1997cited by this paper
• Crystal Structure of the Aequorea victoria Green Fluorescent Protein

  1996cited by this paper
• A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells

  1996cited by this paper
• Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

  1996cited by this paper
• The molecular structure of green fluorescent protein

  1996cited by this paper
• Dual-Luciferase TM Reporter Assay: An Advanced Co-Reporter Technology Integrating Firefly and Renilla Luciferase Assays

  1996cited by this paper
• Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo.

  1996influential reference
• Understanding, improving and using green fluorescent proteins.

  1995cited by this paper
• A High Capacity Assay for Inhibitors of Human Papillomavirus DNA Replication

  1995cited by this paper
• Firefly Luciferase Engineered for Improved Genetic Reporting

  1994cited by this paper
• A comparison of different promoter, enhancer, and cell type combinations in transient transfections.

  1994cited by this paper
• Eukaryotic regulatory elements lurking in plasmid DNA: the activator protein-1 site in pUC.

  1994cited by this paper
• Expression vectors encoding human growth hormone (hGH) controlled by human muscle-specific promoters: prospects for regulated production of hGH delivered by myoblast transfer or intravenous injection.

  1994cited by this paper
• Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis

  1994cited by this paper
• Green fluorescent protein as a marker for gene expression.

  1994cited by this paper
• Photinus pyralis luciferase: vectors that contain a modified luc coding sequence allowing convenient transfer into other systems.

  1994cited by this paper
• Chemiluminescent and bioluminescent reporter gene assays.

  1994cited by this paper
• A sensitive promoter assay based on the transcriptional activator Tat of the HIV-1 virus.

  1994cited by this paper
• Low-light image analysis of transgenic organisms using bacterial luciferase as a marker.

  1994cited by this paper
• Kinetic determination of cellular LacZ expression.

  1994cited by this paper
• Distinct modes of human immunodeficiency virus type 1 proviral latency revealed by superinfection of nonproductively infected cell lines with recombinant luciferase-encoding viruses

  1994cited by this paper
• Selective Inactivation of Eukaryotic β-Galactosidase in Assays for Inhibitors of HIV-1 TAT Using Bacterial β-Galactosidase as a Reporter Enzyme

  1993influential reference
• A polymerase chain reaction-based method for detection and quantification of reporter gene expression in transient transfection assays.

  1993cited by this paper
• Evaluation of a genetically engineered cell line and a histochemical beta-galactosidase assay to detect herpes simplex virus in clinical specimens

  1993cited by this paper
• Monitoring foreign gene expression by a human adenovirus-based vector using the firefly luciferase gene as a reporter.

  1993cited by this paper
• In vitro and in vivo targeting of gene expression to melanoma cells.

  1993cited by this paper
• Functional testing of human dopamine D1 and D5 receptors expressed in stable cAMP-responsive luciferase reporter cell lines.

  1993cited by this paper
• Novel lacZ-based recombination vectors for mammalian cells.

  1993cited by this paper
• Selective inactivation of eukaryotic beta-galactosidase in assays for inhibitors of HIV-1 TAT using bacterial beta-galactosidase as a reporter enzyme.

  1993cited by this paper
• Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.

  1993cited by this paper
• smg/rap1/Krev-1 p21s inhibit the signal pathway to the c-fos promoter/enhancer from c-Ki-ras p21 but not from c-raf-1 kinase in NIH3T3 cells.

  1992cited by this paper
• A Novel Transgenic Mouse Model for the In Vivo Evaluation of Anti-Human Immunodeficiency Virus Type 1 Drugs

  1992cited by this paper
• Use of bacterial and firefly luciferases as reporter genes in DEAE-dextran-mediated transfection of mammalian cells.

  1992cited by this paper
• Post-transcriptional regulation of the human transforming growth factor-beta 1 gene.

  1992cited by this paper
• Reporter constructs with low background activity utilizing the cat gene.

  1992cited by this paper
• Amplified assay of alkaline phosphatase using flavin-adenine dinucleotide phosphate as substrate.

  1992cited by this paper
• GTPase-activating protein SH2-SH3 domains induce gene expression in a Ras-dependent fashion

  1992cited by this paper
• Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells.

  1992cited by this paper
• Primary structure of the Aequorea victoria green-fluorescent protein.

  1992cited by this paper
• GUS protocols: using the GUS gene as a reporter of gene expression.

  1992cited by this paper
• Bioluminescence and chemiluminescence. Current status: Edited by Philip E. Stanley and Larry J. Kricka. Pp. 570. Wiley, Chichester. 1991. £80.00

  1991cited by this paper
• A sensitive method for the detection of beta-galactosidase in transfected mammalian cells.

  1991cited by this paper
• Identification of a transactivating function mapping to the putative immediate-early locus of human herpesvirus 6

  1991cited by this paper
• Poly(A) signals.

  1991cited by this paper
• Whole animal cell sorting of Drosophila embryos.

  1991cited by this paper
• A chemiluminescent assay for quantitation of beta-galactosidase in the femtogram range: application to quantitation of beta-galactosidase in lacZ-transfected cells.

  1991cited by this paper
• A generic intron increases gene expression in transgenic mice

  1991cited by this paper
• Translational potentiation of messenger RNA with secondary structure in Xenopus.

  1991cited by this paper
• Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells.

  1991cited by this paper
• Modulation of firefly luciferase stability and impact on studies of gene regulation.

  1991cited by this paper
• The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

  1991cited by this paper
• Heterologous introns can enhance expression of transgenes in mice.

  1991cited by this paper
• A sensitive method for the detection of beta-galactosidase in transfected mammalian cells.

  1991cited by this paper
• A chemiluminescent assay for quantitation of β-galactosidase in the femtogram range: Application to quantitation of β-galactosidase in lacZ-transfected cells

  1991cited by this paper
• Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA.

  1990cited by this paper
• The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing

  1990cited by this paper
• Reporter genes: application to the study of mammalian gene transcription.

  1990influential reference
• Use of a fluorescent chloramphenicol derivative as a substrate for CAT assays.

  1990cited by this paper
• Do the poly(A) tail and 3' untranslated region control mRNA translation?

  1990cited by this paper
• A novel genetic system to detect protein–protein interactions

  1989cited by this paper
• A simple assay for DNA transfection by incubation of the cells in culture dishes with substrates for beta-galactosidase.

  1989cited by this paper
• Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs

  1989cited by this paper
• Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes.

  1989cited by this paper
• Efficiency of utilization of the simian virus 40 late polyadenylation site: effects of upstream sequences

  1989cited by this paper
• The scanning model for translation: an update

  1989cited by this paper
• Variation in enzymatic transient gene expression assays.

  1989cited by this paper
• Introns in the 3'-untranslated region can inhibit chimeric CAT and beta-galactosidase gene expression.

  1989cited by this paper
• Poly(A), poly(A) binding protein and the regulation of mRNA stability.

  1989cited by this paper
• Mammalian beta-glucuronidase: genetics, molecular biology, and cell biology.

  1989cited by this paper
• The 5' untranslated sequence of the c-sis/platelet-derived growth factor 2 transcript is a potent translational inhibitor

  1988cited by this paper
• pSV00CAT: low background CAT plasmid.

  1988cited by this paper
• Introns increase transcriptional efficiency in transgenic mice.

  1988cited by this paper
• Structure of chloramphenicol acetyltransferase at 1.75-A resolution.

  1988cited by this paper
• Comparison of intron-dependent and intron-independent gene expression

  1988cited by this paper
• Alkaline phosphatase as a reporter enzyme.

  1988cited by this paper
• Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells.

  1988influential reference
• Expression of a human placental alkaline phosphatase gene in transfected cells: use as a reporter for studies of gene expression.

  1988cited by this paper
• A simple phase-extraction assay for chloramphenicol acyltransferase activity.

  1988cited by this paper
• Synthesis and Characterization of Luciferin Derivatives for Use in Bioluminescence Enhanced Enzyme Immunoassays. New Ultrasensitive Detection Systems for Enzyme Immunoassays, I.

  1987cited by this paper
• Firefly luciferase gene: structure and expression in mammalian cells

  1987cited by this paper
• Unidirectional digestion with exonuclease III in DNA sequence analysis.

  1987cited by this paper
• Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer.

  1987cited by this paper
• Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells

  1987cited by this paper

• Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management

  2025cites this paper
• Design Considerations When Creating a High Throughput Screen-Compatible In Vitro Model of Osteogenesis.

  2024cites this paper
• Air sampling and ATP bioluminescence for quantitative detection of airborne microbes.

  2024cites this paper
• Donor-acceptor type π-conjugated 1,3,5-triazine dyes synthesis and its application in cell imaging and liquid food safety detection

  2023cites this paper
• An unbiased AAV-STARR-seq screen revealing the enhancer activity map of genomic regions in the mouse brain in vivo

  2023cites this paper
• Identification and Prioritization of Environmental Organic Pollutants: From an Analytical and Toxicological Perspective.

  2023cites this paper
• Colorectal cancer-associated SNP rs17042479 is involved in the regulation of NAF1 promoter activity

  2022cites this paper
• Design and characterization of synthetic promoters

  2022cites this paper
• CREB is a potential marker associated with drug-induced liver injury: Identification and validation through transcriptome database analysis.

  2022cites this paper
• Beta-Galactosidase as a Transgenic Reporter for the Mapping and Phenotyping of MT1 and MT2 Melatonin Receptor-Expressing Cells.

  2022cites this paper
• PlotXpress, a webtool for normalization and visualization of reporter expression data

  2021cites this paper
• Towards Human Translation of Lentiviral Airway Gene Delivery for Cystic Fibrosis: A One-Month CFTR and Reporter Gene Study in Marmosets

  2021cites this paper
• The Age of Phage: Friend or Foe in the New Dawn of Therapeutic and Biocontrol Applications?

  2021cites this paper
• Development of a one-step synthesized red emission fluorescent probe for sensitive detection of viscosity in vitro and in vivo.

  2021cites this paper
• Current Practice in Bicistronic IRES Reporter Use: A Systematic Review

  2021cites this paper
• A genetic toolkit for co-expression of multiple proteins of diverse physiological implication

  2021cites this paper
• Development of a highly sensitive microplate luminometer using ATP bioluminescence.

  2020cites this paper
• Influence of the reporter vector backbone on 2-deoxyglucose dependent promoter activation.

  2020cites this paper
• Development of a red-emissive two-photon fluorescent probe for sensitive detection of beta-galactosidase in vitro and in vivo

  2020cites this paper
• DNA-Templated Strontium-Doped Calcium Phosphate Nanoparticles for Gene Delivery in Bone Cells.

  2019cites this paper
• Cell states, fates and reprogramming : insights from neural networks, graphical and computational approaches

  2019cites this paper
• A Systematic Review of Drug Metabolism Studies of Plants With Anticancer Properties: Approaches Applied and Limitations

  2019cites this paper
• Cloning Vectors for Introducing Genes into Host Cells

  2018cites this paper
• Unlimited genetic switches for cell-type specific manipulation

  2018cites this paper
• Endogenous Locus Reporter Assays.

  2018cites this paper
• The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs

  2018cites this paper
• mRNA Processing

  2017cites this paper
• Genome-Wide RNAi Screens for RNA Processing Events in Drosophila melanogaster S2 Cells.

  2017cites this paper
• Development of a double-stranded siRNA labelling method by using 99mTc and single photon emission computed tomography imaging

  2017cites this paper
• Single vector non-leaky gene expression system for Drosophila melanogaster

  2017cites this paper
• The Development of a Green Fluorescent Protein Reporter System for Apoptosis Repressor with Caspase Recruitment Domain Promoter Analysis

  2017influential citation
• Endocrine disrupting effects of HIV antiretrovirals in the South African aquatic environment

  2017cites this paper
• Development of protein kinase-responsive photoluminescent probes and cellular regulators of protein phosphorylation

  2016cites this paper
• Multimodal Imaging for DREADD-Expressing Neurons in Living Brain and Their Application to Implantation of iPSC-Derived Neural Progenitors

  2016cites this paper
• Evaluation of Four Tumor-Specific Promoters in Various Cancer Cell Lines

  2016cites this paper
• Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line.

  2016cites this paper
• Induction of HPV-16 Late Gene Expression Through Use of Small Molecule Drugs

  2016cites this paper
• Biochemical assays used for in vitro and in vivo gene expression

  2016cites this paper
• High and low molecular weight hyaluronic acid differentially influence macrophage activation.

  2015cites this paper
• Optimizing Foreign Gene Expression in Recombinant Fowl Adenovirus 9 Vectors

  2015cites this paper
• From GFP to β-lactamase: advancing intact cell imaging for toxins and effectors.

  2015cites this paper
• Pre- and postmortem imaging of transplanted cells

  2015cites this paper
• Validation of Miniaturized One-Step Reverse Transcription qPCR Assays for High-Throughput Screening and Comparison to a Reporter Gene Methodology

  2015cites this paper
• Regulation of neural enhancer activity by Ascl1 and Sox factors

  2014cites this paper
• Melittin-glutathione S-transferase fusion protein exhibits anti-inflammatory properties and minimal toxicity.

  2014cites this paper
• Ectopic Integration Vectors for Generating Fluorescent Promoter Fusions in Bacillus subtilis with Minimal Dark Noise

  2014cites this paper
• DETECTING RNA REGULATORY INTERACTIONS IN BACTERIAL CELLS

  2014cites this paper
• Functional anatomy of distant-acting mammalian enhancers

  2013cites this paper
• A fluorogenic probe for β-galactosidase activity imaging in living cells.

  2013cites this paper
• Establishment and optimization of induced pluripotent stem cell technologies.

  2013cites this paper
• Investigations on the Biotechnology of Microbial Alkaline Phosphatases from North Gujarat Soils

  2013cites this paper
• Development of a bioassay for engineered nanoparticles (ENPs) in the environment

  2013cites this paper
• A cloning vector for creation of Escherichia coli lacZ translational fusions and generation of linear template for chromosomal integration.

  2012cites this paper
• Ferritin as a novel reporter gene for photoacoustic molecular imaging

  2012cites this paper
• Analyse der Genregulation

  2012cites this paper
• How to screen non-viral gene delivery systems in vitro?

  2011cites this paper
• Purification of New β-Galactosidase from Enterococcus faecium MTCC 5153 with Transgalactosylation Activity

  2011cites this paper
• New tools for improving culture strategies for Hepatocyte differentiation

  2011cites this paper
• Radiosynthesis and Evaluation of 5-[125I]Iodoindol-3-yl-β-D- galactopyranoside ([125I]IBDG) as a β-Galactosidase Imaging Radioligand

  2011cites this paper
• Luminescence based enzyme-labeled phage (Phazyme) assays for rapid detection of Shiga toxin producing Escherichia coli serogroups

  2011cites this paper
• Hydrogen peroxide sensors for cellular imaging based on horse radish peroxidase reconstituted on polymer-functionalized TiO₂ nanorods.

  2011cites this paper
• Development and evaluation of a reporter system for prokaryotic cells based on a secreted acid phosphatase from Staphylococcus aureus strain 154

  2010cites this paper
• In Vitro and In Vivo Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs

  2009cites this paper
• Einfluss definierter Umweltbedingungen auf die Expression der Adhäsionsproteine Eap und Emp in S. aureus

  2008cites this paper
• Transient gene delivery for functional enrichment of differentiating embryonic stem cells.

  2008cites this paper
• Radiosynthesis and Evaluation of 5-[125I]Iodoindol-3-yl-β-d-Galactopyranoside as a β-Galactosidase Imaging Radioligand

  2008cites this paper
• Recent Developments of Biological Reporter Technology for Detecting Gene Expression

  2008cites this paper
• Which aspects of HTS are empirically correlated with downstream success?

  2008cites this paper
• Radiosynthesis and evaluation of 5-[125I]iodoindol-3-yl-beta-D-galactopyranoside as a beta-galactosidase imaging radioligand.

  2008cites this paper
• IL-10 Secretion Increases Signal Persistence of HEMA-MMA–Microencapsulated Luciferase-Modified CHO Fibroblasts in Mice

  2008cites this paper
• Enhancement of Glycoprotein Hormone Alpha Subunit Promoter Reporter Gene Activity in Co-transfection Studies – A Cautionary Reminder

  2008cites this paper
• Evaluation of the Staphylococcus aureus Class C Nonspecific Acid Phosphatase (SapS) as a Reporter for Gene Expression and Protein Secretion in Gram-Negative and Gram-Positive Bacteria

  2007cites this paper
• Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice

  2007cites this paper
• Szent István University Postgraduate School of Veterinary Science The transcriptional regulation of the bovine neonatal Fc receptor

  2007cites this paper
• Firefly luciferase terminally degraded by mild heat exposure: implications for reporter assays.

  2006cites this paper
• Detection of hematogenous tumor cell dissemination predicts tumor relapse in patients undergoing surgical resection of colorectal liver metastases

  2005cites this paper
• Enhanced green fluorescent protein as an alternative control reporter to Renilla luciferase.

  2005cites this paper
• PET reporter genes for noninvasive imaging of gene therapy, cell tracking and transgenic analysis.

  2004cites this paper
• Development and characterization of enhanced green fluorescent protein and luciferase expressing cell line for non-destructive evaluation of tissue engineering constructs.

  2004cites this paper
• Accountability and health systems: toward conceptual clarity and policy relevance.

  2004cites this paper
• Reporter Gene Systems for Halophilic Microorganisms

  2004cites this paper
• Molecular Tools for Genetic Analysis of Pseudomonads

  2004cites this paper
• Halophilic Microorganisms

  2004cites this paper
• In vivo imaging of beta-galactosidase activity using far red fluorescent switch.

  2004cites this paper
• In Vivo Imaging of β-Galactosidase Activity Using Far Red Fluorescent Switch

  2004cites this paper
• Gene trapping of the Arabidopsis genome with a firefly luciferase reporter.

  2003cites this paper
• Quantification of β-galactosidase activity after non-viral transfection in vivo

  2003cites this paper
• Quantification of beta-galactosidase activity after non-viral transfection in vivo.

  2003cites this paper
• Plasmid-Based Reporter Genes

  2003cites this paper
• The first reporter gene assay on living cells

  2002cites this paper
• Biolistic transformation of arbuscular mycorrhizal fungi: advances and applications

  2002cites this paper
• Use of Cultured Osteoblastic Cells to Identify and Characterize Transcriptional Regulatory Complexes

  2002cites this paper
• Chloramphenicol Acetyl Transferase

  2002cites this paper
• Reporter bacteriophage assays as a means to detect foodborne pathogenic bacteria

  2002cites this paper
• Mechanosensitive Transcription Factors Involved in Endothelin B Receptor Expression*

  2001cites this paper
• A new reporter gene system suited for cell-free protein synthesis and high-throughput screening in small reaction volumes.

  2001cites this paper
• Cellular screening assays using fluorescence microscopy.

  2001cites this paper
• Purification and some characteristics of a recombinant dimeric rhizobium meliloti beta-galactosidase expressed in escherichia coli.

  2001cites this paper
• Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads.

  2001cites this paper
• Electroporation-mediated delivery of macromolecules to intestinal epithelia

  1999cites this paper