ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
C. Théry,Kenneth W. Witwer,E. Aikawa,M. Alcaraz,Johnathon D. Anderson,R. Andriantsitohaina,A. Antoniou,T. Arab,F. Archer,Georgia K. Atkin-Smith,D. Ayre,J. Bach,Daniel Bachurski,H. Baharvand,L. Balaj,S. Baldacchino,N. Bauer,A. A. Baxter,M. Bebawy,C. Beckham,Apolonija Bedina Zavec,A. Benmoussa,A. Berardi,P. Bergese,E. Bielska,C. Blenkiron,S. Bobis‐Wozowicz,É. Boilard,W. Boireau,A. Bongiovanni,F. Borràs,Steffi Bosch,C. Boulanger,X. Breakefield,Andrew M. Breglio,Meadhbh Á. Brennan,D. Brigstock,A. Brisson,M. Broekman,Jacqueline F. Bromberg,Paulina Bryl-Górecka,S. Buch,A. Buck,D. Burger,Sara Busatto,Dominik Buschmann,B. Bussolati,E. Buzás,James Brian Byrd,G. Camussi,David Carter,Sarah Caruso,L. Chamley,Yu-Ting Chang,Chihchen Chen,Shuai Chen,Lesley Cheng,Andrew R. Chin,A. Clayton,S. Clerici,Alexander Cocks,Emanuele Cocucci,R. Coffey,A. Cordeiro-da-Silva,Y. Couch,F. Coumans,B. Coyle,Rossella Crescitelli,M. F. Criado,C. D’Souza-Schorey,Saumya Das,Amrita Datta Chaudhuri,P. de Candia,E. F. de Santana,O. De Wever,H. D. del Portillo,T. Demaret,Sarah Deville,A. Devitt,Bert Dhondt,D. Di Vizio,L. Dieterich,V. Dolo,A. P. Domínguez Rubio,M. Dominici,M. R. Dourado,Tom A P Driedonks,F. V. Duarte,Heather M. Duncan,R. Eichenberger,K. Ekström,S. El Andaloussi,C. Élie-Caille,U. Erdbrügger,J. Falcón-Perez,Farah Fatima,Jason E. Fish,Miguel Flores-Bellver,András I. Försönits,A. Frelet-Barrand,F. Fricke,G. Fuhrmann,S. Gabrielsson,Ana Gámez-Valero,C. Gardiner,Kathrin Gärtner,R. Gaudin,Y. Gho,B. Giebel,C. Gilbert,M. Gimona,I. Giusti,D. Goberdhan,A. Görgens,S. Gorski,D. Greening,J. Gross,A. Gualerzi,G. Gupta,Dakota Gustafson,A. Handberg,R. Haraszti,P. Harrison,H. Hegyesi,A. Hendrix,A. Hill,F. Hochberg,K. Hoffmann,B. Holder,H. Holthofer,Baharak Hosseinkhani,Guoku Hu,Yiyao Huang,V. Huber,S. Hunt,A. Ibrahim,T. Ikezu,J. Inal,Mustafa Işın,A. Ivanova,Hannah K. Jackson,S. Jacobsen,S. Jay,M. Jayachandran,G. Jenster,Lanzhou Jiang,Suzanne M Johnson,Jennifer C. Jones,A. Jong,T. Jovanović-Talisman,Stephanie Jung,R. Kalluri,S. Kano,S. Kaur,Yumi Kawamura,E. Keller,Delaram Khamari,E. Khomyakova,A. Khvorova,P. Kierulf,K. Kim,T. Kislinger,Mikael Klingeborn,D. Klinke,M. Kornek,Maja Kosanović,Á. Kovács,Eva-Maria Krämer-Albers,S. Krasemann,Mirja Krause,I. Kurochkin,G. Kusuma,Sören Kuypers,S. Laitinen,Scott M Langevin,L. Languino,J. Lannigan,Cecilia Lässer,L. Laurent,Grégory Lavieu,Elisa Lázaro-Ibáñez,S. Le Lay,Myung-Shin Lee,Y. Lee,D. S. Lemos,Metka Lenassi,A. Leszczyńska,Isaac T. S. Li,K. Liao,S. Libregts,E. Ligeti,R. Lim,S. Lim,A. Linē,K. Linnemannstöns,A. Llorente,C. Lombard,M. Lorenowicz,Á. Lörincz,J. Lötvall,Jason Lovett,Michelle C. Lowry,X. Loyer,Q. Lu,B. Lukomska,T. Lunavat,Sybren L. N. Maas,H. Malhi,A. Marcilla,J. Mariani,Javier Mariscal,E. Martens-Uzunova,Lorena Martín-Jaular,M. C. Martı́nez,V. Martins,Mathilde Mathieu,S. Mathivanan,M. Maugeri,L. McGinnis,M. McVey,D. Meckes,Katie L Meehan,I. Mertens,Valentina R Minciacchi,A. Möller,M. Møller Jørgensen,A. Morales-Kastresana,Jess Morhayim,F. Mullier,M. Muraca,L. Musante,V. Mussack,D. Muth,K. Myburgh,T. Najrana,Muhammad Nawaz,I. Nazarenko,P. Nejsum,C. Néri,T. Neri,R. Nieuwland,L. Nimrichter,J. Nolan,E. N. Nolte-‘t Hoen,Nicole Noren Hooten,L. O’Driscoll,Tina M. O’Grady,A. O’Loghlen,T. Ochiya,M. Olivier,A. Ortiz,L. Ortiz,X. Osteikoetxea,O. Østergaard,M. Ostrowski,Jaesung Park,D. Pegtel,H. Peinado,F. Perut,M. Pfaffl,D. Phinney,Bartijn C H Pieters,R. Pink,D. Pisetsky,E. Pogge von Strandmann,Iva Polakovičová,I. Poon,Bonita H. Powell,I. Prada,L. Pulliam,P. Quesenberry,A. Radeghieri,R. Raffai,S. Raimondo,J. Rak,M. Ramirez,G. Raposo,Morsi Rayyan,Neta Regev‐Rudzki,F. Ricklefs,P. Robbins,D. Roberts,S. Rodrigues,E. Rohde,S. Rome,Kasper M A Rouschop,A. Rughetti,Ashley E. Russell,Paula Saá,S. Sahoo,E. Salas-Huenuleo,Catherine Sánchez,J. Saugstad,Meike J. Saul,R. Schiffelers,R. Schneider,T. H. Schøyen,Aaron Scott,Eriomina Shahaj,Shivani Sharma,O. Shatnyeva,Faezeh Shekari,G. Shelke,A. Shetty,K. Shiba,P. Siljander,Andreia M. Silva,Agata Skowronek,Orman L Snyder,R. Soares,Barbara W. Sódar,Carolina Soekmadji,J. Sotillo,P. Stahl,W. Stoorvogel,Shannon L. Stott,E. Strasser,S. Swift,H. Tahara,M. Tewari,Kate Timms,Swasti Tiwari,Rochelle Tixeira,M. Tkach,W. Toh,R. Tomasini,A. Torrecilhas,J. Tosar,Vasilis Toxavidis,L. Urbanelli,P. Vader,B. W. van Balkom,S. G. van der Grein,J. Van Deun,M. V. van Herwijnen,K. Van Keuren-Jensen,Guillaume van Niel,M. V. van Royen,A. V. van Wijnen,M. Vasconcelos,Ivan J. Vechetti,T. Veit,L. Vella,É. Velot,F. Verweij,B. Vestad,J. Viñas,T. Visnovitz,Krisztina V Vukman,Jessica Wahlgren,Dionysios C. Watson,M. Wauben,Alissa M. Weaver,J. Webber,V. Weber,A. Wehman,D. Weiss,J. Welsh,Sebastian Wendt,Å. Wheelock,Z. Wiener,Leonie Witte,Joy Wolfram,A. Xagorari,P. Xander,Jing Xu,Xiaomei Yan,M. Yáñez-Mó,Hang Yin,Y. Yuana,V. Zappulli,J. Zarubova,Vytautas Žėkas,Jian-ye Zhang,Zezhou Zhao,Lei Zheng,Alexander R Zheutlin,Antje M. Zickler,P. Zimmermann,Angela M. Zivkovic,D. Zocco,E. Zuba-Surma
Published 2018 in Journal of Extracellular Vesicles
ABSTRACT
PUBLICATION RECORD
- Publication year
2018
- Venue
Journal of Extracellular Vesicles
- Publication date
2018-11-23
- Fields of study
Not labeled
- Identifiers
- External record
- Source metadata
Semantic Scholar
CITATION MAP
EXTRACTION MAP
CLAIMS
- No claims are published for this paper.
CONCEPTS
- No concepts are published for this paper.