Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging

No abstract is available for this paper.

• Publication year

  2013

• Venue

  Applied and Environmental Microbiology

• Publication date

  2013-08-16

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/AEM.02033-13PMID 23956387PMCID PMC3811213
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Fluorescent proteins function as a prey attractant: experimental evidence from the hydromedusa Olindias formosus and other marine organisms

  2015cited by this paper
• 骨髄異形成症候群のgenome-wide analysis

  2013cited by this paper
• Construction of Improved Tools for Protein Localization Studies in Streptococcus pneumoniae

  2013cited by this paper
• Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP

  2012cited by this paper
• Multi-colored homologs of the green fluorescent protein from hydromedusa Obelia sp.

  2011cited by this paper
• Essential PcsB putative peptidoglycan hydrolase interacts with the essential FtsXSpn cell division protein in Streptococcus pneumoniae D39

  2011cited by this paper
• pSEUDO, a Genetic Integration Standard for Lactococcus lactis

  2011cited by this paper
• Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

  2011cited by this paper
• High‐throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio‐temporal dynamics

  2011cited by this paper
• Rok Regulates yuaB Expression during Architecturally Complex Colony Development of Bacillus subtilis 168

  2010cited by this paper
• Expression and maintenance of ComD–ComE, the two‐component signal‐transduction system that controls competence of Streptococcus pneumoniae

  2010cited by this paper
• Use of Green Fluorescent Protein To Monitor Cell Envelope Stress in Lactococcus lactis

  2009cited by this paper
• Automated Design of Synthetic Ribosome Binding Sites to Precisely Control Protein Expression

  2009cited by this paper
• Cellular localization of choline‐utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems

  2009cited by this paper
• The role of Streptococcus pneumoniae virulence factors in host respiratory colonization and disease

  2008cited by this paper
• Pneumonia research to reduce childhood mortality in the developing world.

  2008cited by this paper
• Bistability, epigenetics, and bet-hedging in bacteria.

  2008cited by this paper
• Plasmid pCS1966, a New Selection/Counterselection Tool for Lactic Acid Bacterium Strain Construction Based on the oroP Gene, Encoding an Orotate Transporter from Lactococcus lactis

  2008cited by this paper
• The orotate transporter encoded by oroP from Lactococcus lactis is required for orotate utilization and has utility as a food-grade selectable marker.

  2007cited by this paper
• Complete Genome Sequence of the Prototype Lactic Acid Bacterium Lactococcus lactis subsp. cremoris MG1363

  2007cited by this paper
• A new integrative reporter plasmid for Streptococcus pneumoniae.

  2007cited by this paper
• Engineering and characterization of a superfolder green fluorescent protein

  2006cited by this paper
• The uses of green fluorescent protein in prokaryotes.

  2005cited by this paper
• Cell population heterogeneity during growth of Bacillus subtilis.

  2005cited by this paper
• A guide to choosing fluorescent proteins

  2005cited by this paper
• The uses of green fluorescent protein in yeasts.

  2005cited by this paper
• Codon bias and heterologous protein expression.

  2004cited by this paper
• Visualization of Differential Gene Expression by Improved Cyan Fluorescent Protein and Yellow Fluorescent Protein Production in Bacillus subtilis

  2004cited by this paper
• Genome2D: a visualization tool for the rapid analysis of bacterial transcriptome data

  2004cited by this paper
• Regulation of endospore formation in Bacillus subtilis

  2003cited by this paper
• The engineering of gene regulatory networks.

  2003cited by this paper
• Hierarchy of sequence-dependent features associated with prokaryotic translation.

  2003cited by this paper
• Regulation of noise in the expression of a single gene

  2002cited by this paper
• Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells

  2002cited by this paper
• The dynamic microbe: green fluorescent protein brings bacteria to light

  2002cited by this paper
• Genome-Wide Analysis of the Stationary-Phase Sigma Factor (Sigma-H) Regulon of Bacillus subtilis

  2002cited by this paper
• Intrinsic noise in gene regulatory networks

  2001cited by this paper
• Fruiting body formation by Bacillus subtilis

  2001cited by this paper
• Genome of the Bacterium Streptococcus pneumoniae Strain R6

  2001cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• In Vivo Effects of Sporulation Kinases on Mutant Spo0A Proteins in Bacillus subtilis

  2001cited by this paper
• Use of Green Fluorescent Protein To Tag Lactic Acid Bacterium Strains under Development as Live Vaccine Vectors

  2000cited by this paper
• Quantitative analysis of gene expression with an improved green fluorescent protein. p6.

  2000cited by this paper
• When the going gets tough: survival strategies and environmental signaling networks in Bacillus subtilis.

  1999cited by this paper
• GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis.

  1999cited by this paper
• Codon usage and lateral gene transfer in Bacillus subtilis.

  1999cited by this paper
• New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria

  1998cited by this paper
• The complete genome sequence…

  1998cited by this paper
• The complete genome sequence of the Gram-positive bacterium Bacillus subtilis

  1997cited by this paper
• FACS-optimized mutants of the green fluorescent protein (GFP).

  1996cited by this paper
• Working toward a new NIOSH.

  1996influential reference
• The recA gene of Streptococcus pneumoniae is part of a competence‐induced operon and controls lysogenic induction

  1995cited by this paper
• Transformation of Lactococcus by electroporation.

  1995cited by this paper
• Use of green fluorescent protein

  1994cited by this paper
• Green fluorescent protein as a marker for gene expression.

  1994cited by this paper
• Primary structure of the Aequorea victoria green-fluorescent protein.

  1992cited by this paper
• Genetic competence in Bacillus subtilis.

  1991cited by this paper
• Molecular biological methods for Bacillus

  1990cited by this paper
• Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis.

  1990influential reference
• Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis.

  1990cited by this paper
• Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363.

  1990influential reference
• Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing

  1983cited by this paper
• REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS

  1961cited by this paper

• Ribosome pausing in amylase producing Bacillus subtilis during long fermentation

  2025cites this paper
• Chromosomal and Plasmid-Based CRISPRi Platforms for Conditional Gene Silencing in Lactococcus lactis

  2025influential citation
• Design, development, and validation of new fluorescent strains for studying oral streptococci

  2025cites this paper
• Unveiling the regulatory network controlling natural transformation in lactococci

  2024cites this paper
• Impact of Protein Aggregates on Sporulation and Germination of Bacillus subtilis

  2023cites this paper
• Efficient plasmid transfer via natural competence in a microbial co‐culture

  2023cites this paper
• Cell-to-cell molecular transfer and environmental stress adaptation in Bacillus subtilis

  2022cites this paper
• Discovery of phage determinants that confer sensitivity to bacterial immune systems

  2022cites this paper
• Sorption of Cellulases in Biofilm Enhances Cellulose Degradation by Bacillus subtilis

  2022cites this paper
• Biofilm cultivation facilitates coexistence and adaptive evolution in an industrial bacterial community

  2022cites this paper
• Programming bacteria to sense environmental DNA for multiplexed pathogen detection

  2022cites this paper
• High-Throughput Time-Lapse Fluorescence Microscopy Screening for Heterogeneously Expressed Genes in Bacillus subtilis

  2022cites this paper
• Programming bacteria for multiplexed DNA detection

  2022cites this paper
• Practical observations on the use of fluorescent reporter systems in Clostridioides difficile

  2022cites this paper
• Cell‐to‐cell non‐conjugative plasmid transfer between Bacillus subtilis and lactic acid bacteria

  2022cites this paper
• Biosensors to Assess the Activity of Promoters and Chaperones in Bacillus subtilis Cells

  2021cites this paper
• Fluorescence-based discrimination of vegetative cells of bacillus strains from Escherichia coli and Saccharomyces cerevisiae

  2021cites this paper
• Examining How Different Carbon Entry Point Affects Recombinant Protein Production from Ethylene Glycol in Bacillus Subtilis

  2021cites this paper
• Practical observations on the use of fluorescent reporter systems in C. difficile

  2021cites this paper
• Riboswitch RSthiT as a Molecular Tool in Lactococcus lactis

  2021cites this paper
• Microbial induced calcium carbonate precipitation study using Bacillus subtilis with application to self-healing concrete preparation and characterization

  2021cites this paper
• Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion

  2021cites this paper
• Employing lytic phage-mediated horizontal gene transfer in Lactococcus lactis

  2020cites this paper
• Development of Lactococcus lactis Biosensors for Detection of Sulfur-Containing Amino Acids

  2020cites this paper
• Dataset for supporting a modular autoinduction device for control of gene expression in Bacillus subtilis

  2020cites this paper
• Development of Lactococcus lactis Biosensors for Detection of Diacetyl

  2020cites this paper
• Enhancement of amino acid production and secretion by Lactococcus lactis using a droplet-based biosensing and selection system

  2020cites this paper
• Another Breaker of the Wall: the Biological Function of the Usp45 Protein of Lactococcus lactis

  2020cites this paper
• Efficient plasmid transfer via natural competence in a synthetic microbial community

  2020cites this paper
• Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup

  2020cites this paper
• Production of Squalene in Bacillus subtilis by Squalene Synthase Screening and Metabolic Engineering

  2020cites this paper
• Microbial competition reduces interaction distances to the low µm-range

  2020cites this paper
• A modular autoinduction device for control of gene expression in Bacillus subtilis.

  2020cites this paper
• Dynamic Membrane Localization of RNase Y in Bacillus subtilis

  2020cites this paper
• Determination of Plasmid pSN1216-29 Host Range and the Similarity in Oligonucleotide Composition Between Plasmid and Host Chromosomes

  2020cites this paper
• Biosensors for the Determination of Promoters and Chaperones Activity in Bacillus subtilis Cells

  2020cites this paper
• High level production of amorphadiene using Bacillus subtilis as an optimized terpenoid cell factory.

  2020influential citation
• A rational approach to improving titer in Escherichia coli‐based cell‐free protein synthesis reactions

  2020cites this paper
• Microbial competition reduces metabolic interaction distances to the low µm-range

  2020cites this paper
• Single-Molecule Dynamics of DNA Receptor ComEA, Membrane Permease ComEC, and Taken-Up DNA in Competent Bacillus subtilis Cells

  2020cites this paper
• Carbohydrate-recognizing regulators from Ruminiclostridium thermocellum work orthogonally in Bacillus subtilis

  2019cites this paper
• Evaluating single-particle tracking by photo-activation localization microscopy (sptPALM) in Lactococcus lactis

  2019cites this paper
• Metabolic Engineering of Bacillus subtilis Toward Taxadiene Biosynthesis as the First Committed Step for Taxol Production

  2019cites this paper
• Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome.

  2019cites this paper
• Engineering Lactococcus lactis for the production of unusual anthocyanins using tea as substrate.

  2019cites this paper
• Fluorescence Tools Adapted for Real-Time Monitoring of the Behaviors of Streptococcus Species

  2019cites this paper
• Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae

  2019cites this paper
• The protein regulator ArgR and the sRNA derived from the 3’-UTR region of its gene, ArgX, both regulate the arginine deiminase pathway in Lactococcus lactis

  2019cites this paper
• Combining Modules for Versatile and Optimal Labeling of Lactic Acid Bacteria: Two pMV158-Family Promiscuous Replicons, a Pneumococcal System for Constitutive or Inducible Gene Expression, and Two Fluorescent Proteins

  2019cites this paper
• Ampicillin-treated Lactococcus lactis MG1363 populations contain persisters as well as viable but non-culturable cells

  2019cites this paper
• Continuous Production of a Shelf‐Stable Living Material as a Biosensor Platform

  2019cites this paper
• Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal

  2019cites this paper
• Differential impact on motility and biofilm dispersal of closely related phosphodiesterases in Pseudomonas aeruginosa

  2019cites this paper
• Molecular Determinants of Substrate Selectivity of a Pneumococcal Rgg-Regulated Peptidase-Containing ABC Transporter

  2019cites this paper
• Fusion of mis‐registered GFP and phase contrast images with convolutional sparse representation and adaptive region energy rule

  2019cites this paper
• The Individual Microbe: Single-Cell Analysis and Agent-Based Modelling

  2019cites this paper
• DEVELOPMENT OF PROTEIN DISPLAY SYSTEMS AND GENETIC TOOLS FOR SPORE-FORMING BACTERIA

  2018cites this paper
• In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria

  2018cites this paper
• Development of a synthetic cumate-inducible gene expression system for Bacillus

  2018cites this paper
• Influence of global gene regulatory networks on single cell heterogeneity of green fluorescent protein production in Bacillus subtilis

  2018cites this paper
• Boosting heterologous protein production yield by adjusting global nitrogen and carbon metabolic regulatory networks in Bacillus subtilis

  2018cites this paper
• Exploring plant‐microbe interactions of the rhizobacteria Bacillus subtilis and Bacillus mycoides by use of the CRISPR‐Cas9 system

  2018cites this paper
• Stable Expression of Modified Green Fluorescent Protein in Group B Streptococci To Enable Visualization in Experimental Systems

  2018influential citation
• RMI-chip: a microfluidics setup for functional imaging of microbial interactions with tree roots

  2018cites this paper
• Optimized fluorescent proteins for the rhizosphere‐associated bacterium Bacillus mycoides with endophytic and biocontrol agent potential

  2018cites this paper
• The Evolution of gene regulation research in Lactococcus lactis

  2017cites this paper
• University of Groningen Disruption of a transcriptional repressor by an IS-element integration leads to the activation of a novel silent cellobiose transporter in Lactococcus lactis MG1363 Solopova,

  2017cites this paper
• Experimental evolution of Lac Lactococcus lactis MG1363 into Lac shows profound adjustment of cellular nitrogen metabolism

  2017cites this paper
• Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum

  2017cites this paper
• Heterogeneity in Bacillus subtilis : growth phase dependent activity and noise

  2017cites this paper
• Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363

  2017cites this paper
• The Bacillus BioBrick Box 2.0: expanding the genetic toolbox for the standardized work with Bacillus subtilis

  2017cites this paper
• Synthetic gene design—The rationale for codon optimization and implications for molecular pharming in plants

  2017cites this paper
• Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2

  2017cites this paper
• Identification of New Degrons in Streptococcus mutans Reveals a Novel Strategy for Engineering Targeted, Controllable Proteolysis

  2017cites this paper
• Analysis of abrB Expression during the Infectious Cycle of Bacillus thuringiensis Reveals Population Heterogeneity

  2017cites this paper
• On the Spatial Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host Overexpressing Membrane Proteins

  2016cites this paper
• Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

  2016cites this paper
• Osmostress in Bacillus subtilis Heterogeneity in the salt-specific stress response of homogenous Bacillus subtilis populations and the effects of osmotic stress on antibiotic resistance

  2016cites this paper
• Integrative bacterial artificial chromosomes for DNA integration into the Bacillus subtilis chromosome.

  2016cites this paper
• Expression of Streptococcus pneumoniae Bacteriocins Is Induced by Antibiotics via Regulatory Interplay with the Competence System

  2016cites this paper
• A part toolbox to tune genetic expression in Bacillus subtilis

  2016cites this paper
• Engineering approaches to investigate pneumococcal gene expression regulation and antibiotic resistance development

  2016cites this paper
• The ParB-parS Chromosome Segregation System Modulates Competence Development in Streptococcus pneumoniae

  2015cites this paper
• Metabolic protein interactions in Bacillus subtilis studied at the single cell level

  2015cites this paper
• Funktionelle Analyse RNA-basierter Regulation des zentralen Energiestoffwechsels in Bacillus licheniformis

  2015cites this paper
• Gene expression platform for synthetic biology in the human pathogen Streptococcus pneumoniae.

  2015cites this paper
• Mining Environmental Plasmids for Synthetic Biology Parts and Devices

  2015cites this paper
• Red Fluorescent Proteins for Gene Expression and Protein Localization Studies in Streptococcus pneumoniae and Efficient Transformation with DNA Assembled via the Gibson Assembly Method

  2015cites this paper
• Single Cell FRET Analysis for the Identification of Optimal FRET-Pairs in Bacillus subtilis Using a Prototype MEM-FLIM System

  2015cites this paper
• Maltose-Dependent Transcriptional Regulation of the mal Regulon by MalR in Streptococcus pneumoniae

  2015cites this paper
• Analysis of gene expression of bifidobacteria using as the reporter an anaerobic fluorescent protein

  2015cites this paper
• Host Glycan Sugar-Specific Pathways in Streptococcus pneumonia: Galactose as a Key Sugar in Colonisation and Infection

  2015cites this paper
• Bright Fluorescent Streptococcus pneumoniae for Live-Cell Imaging of Host-Pathogen Interactions

  2014cites this paper
• Ectopic Integration Vectors for Generating Fluorescent Promoter Fusions in Bacillus subtilis with Minimal Dark Noise

  2014cites this paper
• Overexpression of ribosome recycling factor is responsible for improvement of nucleotide antibiotic-toyocamycin in Streptomyces diastatochromogenes 1628

  2014cites this paper
• Antibiotic-induced replication stress triggers bacterial competence by increasing gene dosage near the origin.

  2014cites this paper
• Live-Cell Imaging Tool Optimization To Study Gene Expression Levels and Dynamics in Single Cells of Bacillus cereus

  2013cites this paper
• A Specific Mutation in the Promoter Region of the Silent cel Cluster Accounts for the Appearance of Lactose-Utilizing Lactococcus lactis MG1363

  2012cites this paper
• Engineering approaches to investigate pneumococcal gene expression regulation and antibiotic resistance development

  year unknowncites this paper