Improved and expanded Q-system reagents for genetic manipulations

The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.

• Publication year

  2014

• Venue

  Nature Methods

• Publication date

  2014-12-11

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1038/nmeth.3250PMID 25581800PMCID 4344399
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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