Repeatability of exercise‐induced changes in mRNA expression and technical considerations for qPCR analysis in human skeletal muscle

Hashim Islam,B. Edgett,Jacob T. Bonafiglia,Talya Shulman,Andrew Ma,J. Quadrilatero,Craig A. Simpson,B. Gurd

Published 2019 in Experimental Physiology

ABSTRACT

What is the central question of this study? Are individual changes in exercise‐induced mRNA expression repeatable (i.e. representative of the true response to exercise rather than random error)? What is the main finding and its importance? Exercise‐induced changes in mRNA expression are not repeatable even under identical experimental conditions, thereby challenging the use of mRNA expression as a biomarker of adaptive potential and/or individual responsiveness to exercise.

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