Characterization of a Novel Polymeric Bioflocculant Produced from Bacterial Utilization of n-Hexadecane and Its Application in Removal of Heavy Metals

A novel polymeric bioflocculant was produced by a bacterium utilizing degradation of n-hexadecane as the energy source. The bioflocculant was produced with a bioflocculating activity of 87.8%. The hydrocarbon degradation was confirmed by gas chromatography-mass spectrometry analysis and was further supported with contact angle measurements for the changes in hydrophobic nature of the culture medium. A specific aerobic degradation pathway followed by the bacterium during the bioflocculant production and hydrocarbon utilization process has been proposed. FT-IR, SEM-EDX, LC/MS, and 1H NMR measurements indicated the presence of carbohydrates and proteins as the major components of the bioflocculant. The bioflocculant was characterized for its carbohydrate monomer constituents and its practical applicability was established for removing the heavy metals (Ni2+, Zn2+, Cd2+, Cu2+, and Pb2+) from aqueous solutions at concentrations of 1–50 mg L-1. The highest activity of the bioflocculant was observed with Ni2+ with 79.29 ± 0.12% bioflocculation efficiency.

• Publication year

  2017

• Venue

  Frontiers in Microbiology

• Publication date

  2017-02-07

• Fields of study

  Medicine, Materials Science, Chemistry, Environmental Science

• Identifiers
  DOI 10.3389/fmicb.2017.00170PMID 28223975PMCID 5293801
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Development of an Efficient Bacterial Consortium for the Potential Remediation of Hydrocarbons from Contaminated Sites

  2016cited by this paper
• Purification and characterization of exopolysaccharide bioflocculant produced by heavy metal resistant Achromobacter xylosoxidans.

  2016cited by this paper
• Wheat Bran Enhances the Cytotoxicity of Immobilized Alcaligenes aquatilis F8 against Microcystis aeruginosa

  2015cited by this paper
• Isolation of Extracellular Polymeric Substances from Biofilms of the Thermoacidophilic Archaeon Sulfolobus acidocaldarius

  2015cited by this paper
• Production of a non-cytotoxic bioflocculant by a bacterium utilizing a petroleum hydrocarbon source and its application in heavy metal removal

  2015influential reference
• Enzymatic Transformations Involved in the Biosynthesis of Microbial Exo‐polysaccharides Based on the Assembly of Repeat Units

  2015cited by this paper
• Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies

  2015cited by this paper
• Innovative biological approaches for monitoring and improving water quality

  2015influential reference
• One Juliet and four Romeos: VeA and its methyltransferases

  2015cited by this paper
• Revealing the characteristics of a novel bioflocculant and its flocculation performance in Microcystis aeruginosa removal

  2015cited by this paper
• Highly mesoporous carbons derived from biomass feedstocks templated with eutectic salt ZnCl2/KCl

  2014cited by this paper
• Characterization of a novel exopolysaccharide with antitumor activity from Lactobacillus plantarum 70810.

  2014influential reference
• Bioflocculant production by Bacillus sp. Gilbert isolated from a marine environment in South Africa

  2014cited by this paper
• Calcium-mediated modulation of Pseudomonas mendocina NR802 biofilm influences the phenanthrene degradation.

  2014cited by this paper
• The use of trimethylsilyl cyanide derivatization for robust and broad-spectrum high-throughput gas chromatography–mass spectrometry based metabolomics

  2013cited by this paper
• Characterization and flocculation properties of biopolymeric flocculant (glycosaminoglycan) produced by Cellulomonas sp. Okoh

  2013cited by this paper
• Determination of petroleum-degrading bacteria isolated from crude oil-contaminated soil in Turkey

  2012cited by this paper
• Characterization of extracellular polymeric substances (EPS) from periphyton using liquid chromatography-organic carbon detection–organic nitrogen detection (LC-OCD-OND)

  2012cited by this paper
• Production and characterization of heavy-metal removing bacterial bioflocculants

  2012cited by this paper
• Isolation and characterization of heavy metal removing bacterial bioflocculants

  2012cited by this paper
• Identification of key constituents and structure of the extracellular polymeric substances excreted by Bacillus megaterium TF10 for their flocculation capacity.

  2011influential reference
• Characterization of a compound bioflocculant produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6

  2011cited by this paper
• Characterization of a compound bioflocculant produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6

  2011cited by this paper
• New trends in removing heavy metals from industrial wastewater

  2011influential reference
• Isolation and Characterization of Environmental Bacteria Capable of Extracellular Biosorption of Mercury

  2011influential reference
• Biosorption of heavy metal ions using wheat based biosorbents--a review of the recent literature.

  2010cited by this paper
• Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms

  2010cited by this paper
• Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review.

  2009cited by this paper
• Alkane Utilization by Rhodococcus Strain NTU‐1 Alone and in Its Natural Association with Bacillus fusiformis L‐1 and Ochrobactrum sp.

  2008cited by this paper
• Microbial flocculation by Bacillus mucilaginosus: Applications and mechanisms.

  2008cited by this paper
• Influence of extracellular polymeric substances on Pseudomonas aeruginosa transport and deposition profiles in porous media.

  2007cited by this paper
• Production and application of a novel bioflocculant by multiple-microorganism consortia using brewery wastewater as carbon source.

  2007cited by this paper
• Zinc sorption to three gram-negative bacteria: combined titration, modeling, and EXAFS study.

  2006cited by this paper
• Influence of hydration of food additive polysaccharides on FT-IR spectra distinction

  2006cited by this paper
• Production of a bioflocculant by Aspergillus parasiticus and its application in dye removal.

  2005cited by this paper
• Alginate is not a significant component of the extracellular polysaccharide matrix of PA14 and PAO1 Pseudomonas aeruginosa biofilms

  2003cited by this paper
• NMR spectroscopy in the study of carbohydrates: Characterizing the structural complexity

  2003influential reference
• Rubredoxins Involved in Alkane Oxidation

  2002cited by this paper
• Aerobic Degradation by Microorganisms

  2001cited by this paper
• Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry.

  2000cited by this paper
• Screening of Bacteria Capable of Producing Bioflocculants from Acetic and Propionic Acids.

  2000cited by this paper
• Technical advance: simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry.

  2000cited by this paper
• Physico-chemistry of initial microbial adhesive interactions--its mechanisms and methods for study.

  1999cited by this paper
• Phenanthrene degradation by microorganisms isolated from a contaminated stream

  1998cited by this paper
• Microbial Transformation and Degradation of Toxic Organic Chemicals

  1996cited by this paper
• Characteristics of a Biopolymer Flocculant Produced by Bacillus sp. PY-90

  1995cited by this paper
• Methods for general and molecular bacteriology

  1994cited by this paper
• Biodegradation of polycyclic aromatic hydrocarbons

  1992cited by this paper
• Microbial flocculant from Alcaligenes cupidus KT201

  1991cited by this paper
• Microbial Flocculant fromAlcaligenes cupidusKT201

  1991cited by this paper
• Screening for and Characteristics of Microbial Flocculants

  1986cited by this paper
• Microbial degradation of xenobiotics

  1986cited by this paper
• A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries.

  1984cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Polycyclic Aromatic Hydrocarbons

  1965cited by this paper

• Direct Utilization of Bacterial Fermentation Broth as Wet Strength Agent and Flocculant on Straw Fiber Composites.

  2026cites this paper
• Hexadecane Biodegradation in Environmental Systems: A Comprehensive Systematic Review and Meta-Analysis

  2026cites this paper
• Natural Antifouling Potential of Fucus vesiculosus and Arthrospira platensis

  2026cites this paper
• Recent advancements in bacterial extracellular polymeric substances based flocculants for wastewater treatment

  2026influential citation
• Applications of silver nanoparticles synthesized from Pichia kudriavzevii bioflocculant isolated from Kombucha tea SCOBY

  2025cites this paper
• A Systematic Review on the Application of Bacteria-Based Bioflocculants in Wastewater Treatment: Trends and Future Advances

  2025cites this paper
• Bioflocculants for microplastics remediation in aquatic environments: Recent advances and biotechnological perspectives

  2025cites this paper
• Structural analysis and flocculation performance of ASP-1: A polysaccharide bioflocculant from asparagus root waste

  2025cites this paper
• Sustainable flocculation system for microalgae harvesting and dye-containing wastewater treatment: Chlorella vulgaris as a bioflocculant source.

  2025cites this paper
• Efficacy of exopolysaccharide in dye-laden wastewater treatment: A comprehensive review.

  2024cites this paper
• Biodegradation of oil-derived hydrocarbons by marine actinobacteria: a systematic review.

  2024cites this paper
• Pioneering technologies over time to rehabilitate crude oil–contaminated ecosystems: a review

  2024influential citation
• Degradation of diesel fuel by Pseudomonas aeruginosa B031 with expression of the alkB gene in a column bioreactor

  2024cites this paper
• Unraveling the Potential of Microbial Flocculants: Preparation, Performance, and Applications in Wastewater Treatment

  2024cites this paper
• Isolation and characterization of Stenotrophomonas pavanii GXUN74707 with efficient flocculation performance and application in wastewater treatment

  2024cites this paper
• Sustainable production of bacterial flocculants by nylon-6,6 microplastics hydrolysate utilizing Brucella intermedia ZL-06.

  2024cites this paper
• Production and characterization of a bioflocculant produced by Proteus mirabilis AB 932526.1 and its application in wastewater treatment and dye removal

  2023cites this paper
• Roles of n-hexadecane in the degradation of dibenzofuran by a biosurfactant-producing bacterium Rhodococcus sp.

  2023cites this paper
• A novel bioflocculant extracted from excess-activated sludge for dye-containing wastewater treatment

  2023cites this paper
• Biodegradation of N-hexadecane using bacterial consortium isolated from seawater contaminated with petroleum

  2023cites this paper
• The Large Molecular Weight Polysaccharide from Wild Cordyceps and Its Antitumor Activity on H22 Tumor-Bearing Mice

  2023cites this paper
• Microbial flocculants as an excellent alternative to synthetic flocculants for industrial application: A comprehensive review

  2023cites this paper
• A polysaccharide-based bioflocculant BP50-2 from banana peel waste: Purification, structure and flocculation performance.

  2022cites this paper
• Hydrocarbonoclastic activity in bacterial biofilms: A systematic study emphasizing pseudomonads

  2022influential citation
• Applications of Bioflocculants for Heavy Metals Removal: A Systematic Review

  2022cites this paper
• Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance

  2022cites this paper
• Production and Characterization of a Bioflocculant from Pichia kudriavzevii MH545928.1 and Its Application in Wastewater Treatment

  2022cites this paper
• Free and Immobilized Microbial Culture–Mediated Crude Oil Degradation and Microbial Diversity Changes Through Taxonomic and Functional Markers in a Sandy Loam Soil

  2022cites this paper
• Microbial remediation of heavy metals from sludge of wastewater treatment plants

  2021cites this paper
• Synthetic algal-bacteria consortia for space-efficient microalgal growth in a simple hydrogel system

  2021cites this paper
• A review of the production process of bacteria-based polymeric flocculants

  2021cites this paper
• Novel cationic chitosan-like bioflocculant from Citrobacter youngae GTC 01314 for the treatment of kaolin suspension and activated sludge

  2021cites this paper
• Recent advances and perspectives in efforts to reduce the production and application cost of microbial flocculants

  2021influential citation
• Enlightening the characteristics of bioflocculant of endophytic actinomycetes from marine algae and its biosorption of heavy metal removal.

  2021cites this paper
• Reusing sago mill effluent as a substrate for bio-based polymeric flocculant fermentation: Optimisation of operational conditions

  2021cites this paper
• Natural flocculants for the treatment of wastewaters containing dyes or heavy metals: A state-of-the-art review

  2021cites this paper
• Isolation and characterisation of bioflocculant-producing bacteria from aquaculture effluent and its performance in treating high turbid water

  2021cites this paper
• Microbial degradation of n-hexadecane using Pseudomonas aeruginosa PU1 isolated from transformer-oil contaminated soil

  2021cites this paper
• Sustainable and efficient technologies for removal and recovery of toxic and valuable metals from wastewater: Recent progress, challenges, and future perspectives.

  2021cites this paper
• Isolation and Optimization of Culture Conditions of a Bioflocculant-Producing Fungi from Kombucha Tea SCOBY

  2021cites this paper
• Transcriptome analysis of potential flocculation-related genes in Streptomyces sp. hsn06 with flocculation activity on Chlorella vulgaris biomass

  2021cites this paper
• Marine Actinobacteria Bioflocculant: A Storehouse of Unique Biotechnological Resources for Wastewater Treatment and Other Applications

  2020cites this paper
• ISOLATION AND IDENTIFICATION OF HEAVY METAL TOLERANT BACTERIA FROM SUGARCANE INDUSTRIAL WASTEWATER

  2020cites this paper
• Application of isolated halophilic microorganisms suspended and immobilized on walnut shell as biocarrier for treatment of oilfield produced water.

  2020cites this paper
• Recent Achievements in Polymer Bio-Based Flocculants for Water Treatment

  2020cites this paper
• Marine Actinobacteria Bioflocculant: A Storehouse of Unique Biotechnological Resources for Wastewater Treatment and Other Applications

  2020cites this paper
• Production of a bioflocculant from ramie biodegumming wastewater using a biomass-degrading strain and its application in the treatment of pulping wastewater.

  2020cites this paper
• Characterization of a novel polymeric bioflocculant from marine actinobacterium Streptomyces sp. and its application in recovery of microalgae

  2020cites this paper
• Production and purification of bioflocculants from newly isolated bacterial species: a comparative decolourization study of cationic and anionic textile dyes

  2020cites this paper
• Optimization of bioflocculant production by bacteria isolated from oil-polluted soil and fermented maize effluent

  2020cites this paper
• Energy-saving preparation of a bioflocculant under high-salt condition by using strain Bacillus sp. and the interaction mechanism towards heavy metals.

  2020cites this paper
• Bioflocculant production using palm oil mill and sago mill effluent as a fermentation feedstock: Characterization and mechanism of flocculation.

  2020cites this paper
• Bioconversion of citrus peel wastes into bioflocculants and their application in the removal of microcystins.

  2020cites this paper
• Bioflocculant production and heavy metal sorption by metal resistant bacterial isolates from gold mining soil.

  2019cites this paper
• Hexadecane and Hexadecane-Degrading Bacteria: Mechanisms of Interaction

  2019cites this paper
• Bioconversion of kitchen wastes into bioflocculant and its pilot-scale application in treating iron mineral processing wastewater.

  2019cites this paper
• Microwave assisted synthesis and characterization of a novel bio-based flocculant from dextran and chitosan.

  2019cites this paper
• Role of Bacterial Bioflocculant on Antibiofilm Activity and Metal Removal Efficiency

  2019cites this paper
• Production and Flocculating Performance of Bioflocculant by Bacterial Strain and its Application for Municipal Wastewater Treatment

  2019cites this paper
• Simultaneous nitriles degradation and bioflocculant production by immobilized K. oxytoca strain in a continuous flow reactor.

  2019cites this paper
• A cost-effective and environmentally sustainable process for phycoremediation of oil field formation water for its safe disposal and reuse

  2019cites this paper
• OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

  2019cites this paper
• A novel efficient bioflocculant QZ-7 for the removal of heavy metals from industrial wastewater

  2019cites this paper
• Sequential treatment of paper mill effluent with modified Fenton oxidation and bioflocculation

  2019cites this paper
• Enhancement of heavy metals removal by microbial flocculant produced by Paenibacillus polymyxa combined with an insufficient hydroxide precipitation

  2019cites this paper
• Removal of heavy metals in lake water using bioflocculant produced by Bacillus subtilis

  2019cites this paper
• Microbial Flocculants as an Alternative to Synthetic Polymers for Wastewater Treatment: A Review

  2018cites this paper
• Advances in exopolysaccharides based bioremediation of heavy metals in soil and water: A critical review.

  2018cites this paper
• Bioflocculants from isolated stains: A research update

  2018cites this paper
• Unravelling the characteristics of a heteropolysaccharide–protein from an Haloarchaeal strain with flocculation effectiveness in heavy metals and dyes removal

  2018cites this paper
• Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest

  2017cites this paper
• Production, Characterization and Effect of Cultural Condition on Bioflocculant Produced by Alcaligenes aquatilis AP4

  2017cites this paper
• Recent advances in polysaccharide bio-based flocculants.

  2017cites this paper
• Advancements in natural bio-flocculants for water treatment

  year unknowncites this paper