Synthesis of Diblock copolymer poly-3-hydroxybutyrate -block-poly-3-hydroxyhexanoate [PHB-b-PHHx] by a β-oxidation weakened Pseudomonas putida KT2442

BackgroundBlock polyhydroxyalkanoates (PHA) were reported to be resistant against polymer aging that negatively affects polymer properties. Recently, more and more attempts have been directed to make PHA block copolymers. Diblock copolymers PHB-b-PHHx consisting of poly-3-hydroxybutyrate (PHB) block covalently bonded with poly-3-hydroxyhexanoate (PHHx) block were for the first time produced successfully by a recombinant Pseudomonas putida KT2442 with its β-oxidation cycle deleted to its maximum.ResultsThe chloroform extracted polymers were characterized by nuclear magnetic resonance (NMR), thermo- and mechanical analysis. NMR confirmed the existence of diblock copolymers consisting of 58 mol% PHB as the short chain length block with 42 mol% PHHx as the medium chain length block. The block copolymers had two glass transition temperatures (Tg) at 2.7°C and −16.4°C, one melting temperature (Tm) at 172.1°C and one cool crystallization temperature (Tc) at 69.1°C as revealed by differential scanning calorimetry (DSC), respectively. This is the first microbial short-chain-length (scl) and medium-chain-length (mcl) PHA block copolymer reported.ConclusionsIt is possible to produce PHA block copolymers of various kinds using the recombinant Pseudomonas putida KT2442 with its β-oxidation cycle deleted to its maximum. In comparison to a random copolymer poly-3-hydroxybutyrate-co-3-hydroxyhexanoate (P(HB-co-HHx)) and a blend sample of PHB and PHHx, the PHB-b-PHHx showed improved structural related mechanical properties.

• Publication year

  2012

• Venue

  Microbial Cell Factories

• Publication date

  2012-04-05

• Fields of study

  Medicine, Materials Science, Chemistry, Environmental Science

• Identifiers
  DOI 10.1186/1475-2859-11-44PMID 22480145PMCID 3442986
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Microbial Cell Factories

  2012cited by this paper
• Plastics derived from biological sources: present and future: a technical and environmental review.

  2012cited by this paper
• Characterisation of copolymer, poly (hydroxybutyrate-co-hydroxyvalerate) (PHB-co-PHV) produced by Halomonas campisalis (MCM B-1027), its biodegradability and potential application.

  2011cited by this paper
• Microbial production of polyhydroxyalkanoate block copolymer by recombinant Pseudomonas putida

  2011cited by this paper
• Biosynthesis of poly(3-hydroxydecanoate) and 3-hydroxydodecanoate dominating polyhydroxyalkanoates by β-oxidation pathway inhibited Pseudomonas putida.

  2011cited by this paper
• Linking ecology with economy: Insights into polyhydroxyalkanoate‐producing microorganisms

  2011cited by this paper
• Biosynthesis of polyhydroxyalkanoate homopolymers by Pseudomonas putida

  2011influential reference
• Biosynthesis and characterization of poly(3-hydroxydodecanoate) by β-oxidation inhibited mutant of Pseudomonas entomophila L48.

  2011cited by this paper
• Biosynthesis and characterization of polyhydroxyalkanoate block copolymer P3HB-b-P4HB.

  2011cited by this paper
• Polyhydroxyalkanoates as a source of chemicals, polymers, and biofuels.

  2011cited by this paper
• Polyhydroxyalkanoates: bioplastics with a green agenda.

  2010cited by this paper
• Current trends in biodegradable polyhydroxyalkanoates.

  2010cited by this paper
• Polymer Data Handbook

  2009cited by this paper
• The influence of copolymer ratio and drug loading level on the biocompatibility of P(3HB-co-4HB) synthesized by Cupriavidus sp. (USMAA2-4)

  2008cited by this paper
• Production of polyhydroxyalkanoates with high 3-hydroxydodecanoate monomer content by fadB and fadA knockout mutant of Pseudomonas putida KT2442.

  2007cited by this paper
• Investigating the structure-property relationship of bacterial PHA block copolymers.

  2007cited by this paper
• Polyhydroxyalkanoates: biodegradable polymers with a range of applications

  2007cited by this paper
• The effect of D,L-beta-hydroxybutyric acid on cell death and proliferation in L929 cells.

  2006cited by this paper
• Sequence distribution in microbial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-polyesters determined by NMR and MS.

  2006cited by this paper
• Bacterial synthesis of PHA block copolymers.

  2006influential reference
• Enhanced vascular-related cellular affinity on surface modified copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx).

  2005cited by this paper
• Block copolymers in tomorrow's plastics

  2005cited by this paper
• Polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas stutzeri 1317 had different substrate specificities.

  2004cited by this paper
• Polyhydroxyalkanoate (PHA) scaffolds with good mechanical properties and biocompatibility.

  2003cited by this paper
• Biosynthesis and properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) polymers.

  2002cited by this paper
• Effect of lipase treatment on the biocompatibility of microbial polyhydroxyalkanoates

  2002cited by this paper
• Enhanced production of D-(-)-3-hydroxybutyric acid by recombinant Escherichia coli.

  2002cited by this paper
• Effect of surface treatment on the biocompatibility of microbial polyhydroxyalkanoates.

  2002cited by this paper
• Characterization of 13 kDa granule-associated protein in Aeromonas caviae and biosynthesis of polyhydroxyalkanoates with altered molar composition by recombinant bacteria.

  2001cited by this paper
• PCR cloning of type II polyhydroxyalkanoate biosynthesis genes from two Pseudomonas strains.

  2001cited by this paper
• Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters

  2000cited by this paper
• Chemical composition distribution of bacterial copolyesters.

  1999cited by this paper
• Mechanical properties of composites using ultrafine electrospun fibers

  1999cited by this paper
• Fractionation and Characterization of Microbial Polyesters Containing 3-Hydroxybutyrate and 4-Hydroxybutyrate Repeat Units

  1997cited by this paper
• Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae

  1997cited by this paper
• Bacterial polyhydroxyalkanoates

  1996cited by this paper
• Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans.

  1996cited by this paper
• Copyright © 1997, American Society for Microbiology Cloning and Analysis of the Poly(3-Hydroxybutyrate-co-3-

  1996cited by this paper
• Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)

  1995cited by this paper
• Complex composition distribution of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

  1995cited by this paper
• Diversity of bacterial polyhydroxyalkanoic acids

  1995cited by this paper
• Physical properties and biodegradability of microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)

  1994cited by this paper
• MICROBIAL SYNTHESIS AND PROPERTIES OF POLY(3-HYDROXYBUTYRATE-CO-4-HYDROXYBUTYRATE) IN COMAMONAS ACIDOVORANS

  1994cited by this paper
• Composition range of crystal phase transition of isodimorphism in poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

  1993cited by this paper
• Crystallization phenomena in bacterial poly[(R)-3-hydroxybutyrate]: 2. Embrittlement and rejuvenation

  1993cited by this paper
• Microstructure of bacterially synthesized poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

  1989cited by this paper
• The microstructures of commercially available poly(3-hydroxybutyrate-co-3-hydroxyvalerate)s

  1989cited by this paper
• Isodimorphism in bacterial poly(β-hydroxybutyrate-co-β-hydroxyvalerate)

  1986cited by this paper
• Nuclear magnetic resonance studies on poly(β-hydroxybutyrate) and a copolyester of β-hydroxybutyrate and β-hydroxyvalerate isolated from Alcaligenes eutrophus H16

  1986cited by this paper

• A Chemocatalytic Route to Stereoregular Poly(3-hydroxyhexanoate) and Its Statistical and Tri-Block Copolymers.

  2026cites this paper
• Chemistry of bioplastic production by Alcaligenes faecalis NSBN10 using cane molasses: optimization and characterization

  2025cites this paper
• Environmentally friendly upcycling of PHA-based copolymers.

  2025cites this paper
• Sustainable Open Production of Short–Medium-Chain Length Polyhydroxyalkanoates from Crude Glycerol in Batch and Fed-Batch Bioreactors by Pseudomonas frigusceleri MPC6

  2025cites this paper
• Evaluation of Blended Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Properties Containing Various 3HHx Monomers

  2024cites this paper
• Identification of Oil-Loving Cupriavidus necator BM3-1 for Polyhydroxyalkanoate Production and Assessing Contribution of Exopolysaccharide for Vegetable Oil Utilization

  2024cites this paper
• Engineering Pseudomonas chlororaphis HT66 for the Biosynthesis of Copolymers Containing 3-Hydroxybutyrate and Medium-Chain-Length 3-Hydroxyalkanoates.

  2024cites this paper
• Synthesis and physical properties of polyhydroxyalkanoate (PHA)-based block copolymers: A review.

  2024cites this paper
• Toward the production of block copolymers in microbial cells: achievements and perspectives

  2024cites this paper
• Production of polyhydroxybutyrate (PHB), a biodegradable polymer from seaweed biomass using novel bacterial isolates

  2024cites this paper
• Tuning the rheological and mechanical properties of biodegradable PHBH/PLA/PVAc ternary blends via PVAc content control

  2024cites this paper
• Make it or break it: A review on PHA synthase and depolymerase proteins

  2024cites this paper
• Rates of increase and decrease for each block of poly(3-hydroxybutyrate)(P3HB)-b-poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(P3HBV) and P3HBV-b-P3HB during biosynthesis by Ralstonia Eutropha

  2024cites this paper
• Understanding the Reaction of Hydroxy-Terminated Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Random Copolymers with a Monoisocyanate

  2023cites this paper
• Recent advances on the systems metabolically engineered Pseudomonas species as versatile biosynthetic platforms for the production of polyhydroxyalkanoates

  2023cites this paper
• Construction of micelles and hollow spheres via the self-assembly behavior of poly(styrene-alt-pHPMI) copolymers with poly(4-vinylpyridine) derivatives mediated by hydrogen bonding interactions.

  2023cites this paper
• Synthesis and characterisation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) multi-block copolymers comprising blocks of differing 3-hydroxyvalerate contents

  2023cites this paper
• Synthesis and Characterisation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-b-poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Multi-Block Copolymers Produced Using Diisocyanate Chemistry

  2023cites this paper
• Polyhydroxyalkanoate biosynthesis and optimisation of thermophilic Geobacillus stearothermophilus strain K4E3_SPR_NPP

  2023cites this paper
• Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises.

  2023cites this paper
• Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH): synthesis, properties, and applications - A Review

  2022cites this paper
• Characterisation of end groups of hydroxy-functionalised scl-PHAs prepared by transesterification using ethylene glycol

  2022cites this paper
• Innovative Solutions and Challenges to Increase the use of Poly(3-hydroxybutyrate) in Food Packaging and Disposables

  2022cites this paper
• Poly(3-mercapto-2-methylpropionate), a Novel α-Methylated Bio-Polythioester with Rubber-like Elasticity, and Its Copolymer with 3-hydroxybutyrate: Biosynthesis and Characterization

  2022cites this paper
• Supercritical CO2 Foaming of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)

  2022cites this paper
• Directed Evolution of Sequence-Regulating Polyhydroxyalkanoate Synthase to Synthesize a Medium-Chain-Length-Short-Chain-Length (MCL-SCL) Block Copolymer.

  2022cites this paper
• Microbial production of medium-chain length polyhydroxyalkanoates

  2021cites this paper
• Tailor‐Made Polyhydroxyalkanoates by Reconstructing Pseudomonas Entomophila

  2021cites this paper
• Metabolic Engineering of Pseudomonas

  2021cites this paper
• Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production

  2021cites this paper
• A Review of the Applications and Biodegradation of Polyhydroxyalkanoates and Poly(lactic acid) and Its Composites

  2021cites this paper
• Polyhydroxyalkanoates (PHAs): Biopolymers for Biofuel and Biorefineries

  2021cites this paper
• Polyhydroxyalkanoates (PHA) toward cost competitiveness and functionality

  2020cites this paper
• Metabolic engineering for the synthesis of polyesters: A 100-year journey from polyhydroxyalkanoates to non-natural microbial polyesters.

  2020cites this paper
• Effect of the Molecular Structure of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-3HV)) Produced from Mixed Bacterial Cultures on Its Crystallization and Mechanical Properties.

  2020cites this paper
• Microbial Polyhydroxyalkanoates and Nonnatural Polyesters

  2020cites this paper
• Biodiesel production and parameter optimization: An approach to utilize residual ash from sugarcane leaf, a novel heterogeneous catalyst, from Calophyllum inophyllum oil

  2020cites this paper
• Characterization of Polyhydroxyalkanoate Produced by Halomonas venusta KT832796

  2020cites this paper
• Advances in Plant Based Biologics

  2019cites this paper
• Discarded Egg Yolk as an Alternate Source of Poly(3-Hydroxybutyrate-co-3-hydroxyhexanoate).

  2019cites this paper
• Chemical and Biochemical Engineering Approaches in Manufacturing Polyhydroxyalkanoate (PHA) Biopolyesters of Tailored Structure with Focus on the Diversity of Building Blocks

  2019cites this paper
• Production and characterization of biodegradable films of a novel polyhydroxyalkanoate (PHA) synthesized from peanut oil

  2019cites this paper
• Pseudomonas Species as Producers of Eco-friendly Polyhydroxyalkanoates

  2019cites this paper
• Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers

  2019cites this paper
• Microbial Polyhydroxyalkanoates: Current Status and Future Prospects

  2019cites this paper
• Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) terpolymer production from volatile fatty acids using engineered Ralstonia eutropha.

  2019cites this paper
• jmbReview Discarded Egg Yolk as an Alternate Source of Poly ( 3-Hydroxybutyrate-co3-Hydroxyhexanoate )

  2019cites this paper
• Metabolic engineering of Pseudomonas mendocina NK-01 for enhanced production of medium-chain-length polyhydroxyalkanoates with enriched content of the dominant monomer.

  2019cites this paper
• Optimization of the culture conditions for production of Polyhydroxyalkanoate and its characterization from a new Bacillus cereus sp. BNPI-92 strain, isolated from plastic waste dumping yard.

  2019cites this paper
• The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

  2019cites this paper
• Polymer composites for sustainable 3D printing materials

  2019cites this paper
• Production Strategies for Commercialization of PHA

  2019cites this paper
• Functional Approach for the Development and Production of Novel Extreme Biocatalysts

  2019cites this paper
• Microbial Biofilm Membranes for Water Remediation and Photobiocatalysis

  2019cites this paper
• Synthetic Biology and Metabolic Engineering Approaches for Improved Production and Recovery of Bacterial Polyhydroxyalkanoates

  2019cites this paper
• Bacterial Cell Surface Display

  2019cites this paper
• New Trends in the Biomanufacturing of Green Surfactants: Biobased Surfactants and Biosurfactants

  2019cites this paper
• Sequestering of CO2 to Value-Added Products through Various Biological Processes

  2019cites this paper
• Functional Oligosaccharides: Production and Action

  2019cites this paper
• Rational Design of Next-Generation Therapeutic Antibodies Using Protein Engineering Tools

  2019cites this paper
• Characteristics and Applications of Biodiesels and Design of Reactors for Their Industrial Manufacture

  2019cites this paper
• Material and Methods of Bacterial Sensing in the Process of Pharmaceutical Biomanufacturing

  2019cites this paper
• Cyanobacterial Cell Factories for Improved Carotenoid Biosynthesis through a Synthetic Biology Approach

  2019cites this paper
• Electrospinning: An Efficient Biopolymer-Based Micro- and Nanofibers Fabrication Technique

  2019cites this paper
• Yeasts as Microbial Factories for Production of Recombinant Human Interferon Alpha 2b of Therapeutic Importance

  2019cites this paper
• Polyhydroxyalkanoates (PHAs) in Industrial Applications

  2019cites this paper
• Preface

  2019cites this paper
• Next Generation Biomanufacturing Technologies

  2019cites this paper
• Quorum sensing as a potential target for increased production of rhamnolipid biosurfactant in Burkholderia thailandensis E264

  2019cites this paper
• Title, Copyright, Foreword

  2019cites this paper
• Editors’ Biographies

  2019cites this paper
• Synthetic Biology of Polyhydroxyalkanoates (PHA).

  2018cites this paper
• Dynamic Changes of Intracellular Monomer Levels Regulate Block Sequence of Polyhydroxyalkanoates in Engineered Escherichia coli.

  2018cites this paper
• Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism.

  2018cites this paper
• A non-naturally-occurring P(3HB-co-3HAMCL) is produced by recombinant Pseudomonas sp. from an unrelated carbon source.

  2018cites this paper
• Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology.

  2018cites this paper
• Heterologous expression of phaC2 gene and poly-3-hydroxyalkanoate production by recombinant Cupriavidus necator strains using canola oil as carbon source.

  2018cites this paper
• Engineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis

  2017cites this paper
• Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

  2017cites this paper
• Production of Polyhydroxyalkanoates

  2017cites this paper
• Recent advances of using polyhydroxyalkanoate-based nanovehicles as therapeutic delivery carriers.

  2017cites this paper
• Microbial Synthesis of Polyhydroxyalkanoates: Diversification

  2017cites this paper
• Polyhydroxyalkanoates: Microbial Synthesis and Applications

  2017cites this paper
• Polyhydroxyalkanoates: Sustainability, Production, and Industrialization

  2017cites this paper
• Construção de mutantes de Pseudomonas abrigando diferentes PHA sintases em seu genoma, para produção de 3HB-co-3HAMCL.

  2017cites this paper
• Effective recovery of poly‐β‐hydroxybutyrate (PHB) biopolymer from Cupriavidus necator using a novel and environmentally friendly solvent system

  2016cites this paper
• Recent strategies for efficient production of polyhydroxyalkanoates by micro‐organisms

  2016cites this paper
• Synthetic routes to degradable copolymers deriving from the biosynthesized polyhydroxyalkanoates: A mini review

  2016cites this paper
• Modification and Potential Application of Short-Chain-Length Polyhydroxyalkanoate (SCL-PHA)

  2016cites this paper
• Microbial synthesis of a novel terpolyester P(LA‐co‐3HB‐co‐3HP) from low‐cost substrates

  2016cites this paper
• [Progress on polyhydroxyalkanoates (PHA)].

  2016cites this paper
• A holistic view of polyhydroxyalkanoate metabolism in Pseudomonas putida.

  2016cites this paper
• Synthetic biology of microbes synthesizing polyhydroxyalkanoates (PHA)

  2016cites this paper
• Production of poly(3-hydroxypropionate) and poly(3-hydroxybutyrate-co-3-hydroxypropionate) from glucose by engineering Escherichia coli.

  2015cites this paper
• Engineering Biosynthesis Mechanisms for Diversifying Polyhydroxyalkanoates.

  2015cites this paper
• Potential and Prospects of Continuous Polyhydroxyalkanoate (PHA) Production

  2015cites this paper
• Biosynthesis, characterization, and hemostasis potential of tailor-made poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Haloferax mediterranei.

  2015cites this paper
• The 'PHAome'.

  2015cites this paper
• Poly(hydroxy alkanoate)s in Medical Applications.

  2015cites this paper
• Produção de Polihidroxialcanoatos (PHA) por Halomonas sp. HG01

  2015cites this paper