Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces

Streptomyces are best known for producing antimicrobial secondary metabolites, but they are also recognized for their contributions to biomass utilization. Despite their importance to carbon cycling in terrestrial ecosystems, our understanding of the cellulolytic ability of Streptomyces is currently limited to a few soil-isolates. Here, we demonstrate the biomass-deconstructing capability of Streptomyces sp. SirexAA-E (ActE), an aerobic bacterium associated with the invasive pine-boring woodwasp Sirex noctilio. When grown on plant biomass, ActE secretes a suite of enzymes including endo- and exo-cellulases, CBM33 polysaccharide-monooxygenases and hemicellulases. Genome-wide transcriptomic and proteomic analyses and biochemical assays have revealed the key enzymes used to deconstruct crystalline cellulose, other pure polysaccharides and biomass. The mixture of enzymes obtained from growth on biomass has biomass-degrading activity comparable to a cellulolytic enzyme cocktail from the fungus Trichoderma reesei and thus provides a compelling example of high cellulolytic capacity in an aerobic bacterium.

• Publication year

  2013

• Venue

  Scientific Reports

• Publication date

  2013-01-07

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/srep01030PMID 23301151PMCID 3538285
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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