Regulation of cell surface GLUT1, GLUT3, and GLUT4 by insulin and IGF‐I in L6 myotubes

The effects of insulin and IGF‐I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis‐mannose phololabel (ATB‐BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the translocation of all three glucose transporter isoforms to the plasma membrane fraction from the light microsome fraction, resulting in equal molar quantities on the cell surface. IGF‐I stimulated a similar translocation of all isoforms, augmented by an increase in surface GLUT3 as assessed by ATB‐BMPA.

• Publication year

  1995

• Venue

  FEBS Letters

• Publication date

  1995-07-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0014-5793(95)00589-2PMID 7615080
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The GLUT3 glucose transporter is the predominant isoform in primary cultured neurons: assessment by biosynthetic and photoaffinity labelling.

  1994cited by this paper
• Regulation of the GLUT1 glucose transporter in cultured myocytes: total number and subcellular distribution as determined by photoaffinity labelling.

  1994cited by this paper
• Insulin stimulation of glucose transport activity in rat skeletal muscle: increase in cell surface GLUT4 as assessed by photolabelling.

  1994cited by this paper
• Glucose transporter proteins in brain

  1994cited by this paper
• Structure and function of mammalian facilitative sugar transporters.

  1993cited by this paper
• Kinetics of GLUT1 and GLUT4 glucose transporters expressed in Xenopus oocytes.

  1993cited by this paper
• The glucose transporter family: structure, function and tissue-specific expression.

  1993cited by this paper
• Characterization of GLUT3 protein expressed in Chinese hamster ovary cells.

  1992cited by this paper
• Photolabelling of the liver-type glucose-transporter isoform GLUT2 with an azitrifluoroethylbenzoyl-substituted bis-D-mannose.

  1992cited by this paper
• Development regulation of the subcellular distribution and glycosylation of GLUT1 and GLUT4 glucose transporters during myogenesis of L6 muscle cells.

  1992cited by this paper
• Cell surface accessibility of GLUT4 glucose transporters in insulin-stimulated rat adipose cells. Modulation by isoprenaline and adenosine.

  1992cited by this paper
• Detection of the GLUT3 facilitative glucose transporter in rat L6 muscle cells: regulation by cellular differentiation, insulin and insulin-like growth factor-I.

  1992cited by this paper
• Kinetic resolution of the separate GLUT1 and GLUT4 glucose transport activities in 3T3-L1 cells.

  1992cited by this paper
• Acute and long‐term effects of insulin‐like growth factor I on glucose transporters in muscle cells Translocation and biosynthesis

  1992cited by this paper
• Exofacial photolabelling of the human erythrocyte glucose transporter with an azitrifluoroethylbenzoyl-substituted bismannose.

  1990cited by this paper
• Cell surface labeling of glucose transporter isoform GLUT4 by bis-mannose photolabel. Correlation with stimulation of glucose transport in rat adipose cells by insulin and phorbol ester.

  1990cited by this paper
• Insulin regulation of the two glucose transporters in 3T3-L1 adipocytes.

  1990cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Retention of differentiation potentialities during prolonged cultivation of myogenic cells.

  1968cited by this paper

• Photoperiodic changes in hippocampal neurogenesis and plasma metabolomic profiles in relation to depression-like behavior in mice.

  2021cites this paper
• Dynamic modulation of glucose utilisation by glucocorticoid rhythms in health and disease

  2020cites this paper
• Potential role of insulin on the pathogenesis of depression

  2020cites this paper
• Effects of dietary carbohydrate levels on growth, glucose tolerance, glucose homeostasis and GLUT4 gene expression in Tilapia nilotica

  2018cites this paper
• SLC2A3 single‐nucleotide polymorphism and duplication influence cognitive processing and population‐specific risk for attention‐deficit/hyperactivity disorder

  2017cites this paper
• Blood-Brain Glucose Transfer in Alzheimer’s disease: Effect of GLP-1 Analog Treatment

  2017cites this paper
• near term promotes placental transport and nutrient partitioning Early treatment of the pregnant guinea pig with IGFs

  2016cites this paper
• Kinome Screen Identifies PFKFB3 and Glucose Metabolism as Important Regulators of the Insulin/Insulin-like Growth Factor (IGF)-1 Signaling Pathway*

  2015cites this paper
• Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows.

  2015cites this paper
• PFKFB3 and insulin signalling

  2015cites this paper
• Insulin Induces an Increase in Cytosolic Glucose Levels in 3T3-L1 Cells with Inhibited Glycogen Synthase Activation

  2014cites this paper
• Thyroid hormone inhibition in L6 myoblasts of IGF-1-mediated

  2014cites this paper
• Thyroid hormone inhibition in L6 myoblasts of IGF-I-mediated glucose uptake and proliferation: new roles for integrin αvβ3.

  2014cites this paper
• Green tea (-)-epigallocatechin gallate suppresses IGF-I and IGF-II stimulation of 3T3-L1 adipocyte glucose uptake via the glucose transporter 4, but not glucose transporter 1 pathway.

  2014cites this paper
• ATM and GLUT1-S490 Phosphorylation Regulate GLUT1 Mediated Transport in Skeletal Muscle

  2013cites this paper
• Efeito da insulina na competência de oócitos bovinos maturados em meio definido

  2013cites this paper
• Genome-wide screenings in attention-deficit/hyperactivity disorder (ADHD): investigation of novel candidate genes SLC2A3 and LPHN3 Genomweite Untersuchungen des Aufmerksamkeitsdefizit/ Hyperaktivitätssyndroms (ADHS): Analyse der neuen Kandidatengene SLC2A3 und LPHN3

  2013cites this paper
• Pentobarbital inhibits glucose uptake, but not water transport by glucose transporter type 3

  2012cites this paper
• Investigation of the Expression of Glucose Transporter Proteins in Human Cancer Cells

  2012cites this paper
• IGF-1 induces hypoxia-inducible factor 1α-mediated GLUT3 expression through PI3K/Akt/mTOR dependent pathways in PC12 cells.

  2012cites this paper
• Effects of somatic cell nuclear transfer on transcripts related to IGF signaling, EGF signaling and cell adhesion during the peri-implantation period in cattle

  2012cites this paper
• Metabolic Effects of Insulin and IGFs on Gilthead Sea Bream (Sparus aurata) Muscle Cells

  2012cites this paper
• Normal muscle glucose uptake in mice deficient in muscle GLUT4.

  2012cites this paper
• Molecular Basis of Signaling Specificity of Insulin and IGF Receptors: Neglected Corners and Recent Advances

  2011cites this paper
• GLUT4 in murine bone growth: from uptake and translocation to proliferation and differentiation.

  2011cites this paper
• Insulin and Insulin-like Growth Factor-1 Receptors Act as Ligand-specific Amplitude Modulators of a Common Pathway Regulating Gene Transcription*

  2010cites this paper
• Fatty acids inhibit insulin-mediated glucose transport associated with actin remodeling in rat L6 muscle cells

  2010cites this paper
• Investigations of cellular glucose transport and its regulation under the influence of insulin in human peripheral blood lymphocytes.

  2010cites this paper
• Insulin-like growth factor I (IGF-I) is a more potent regulator of gene expression than insulin in primary human myoblasts and myotubes.

  2009cites this paper
• Berberine improves free-fatty-acid-induced insulin resistance in L6 myotubes through inhibiting peroxisome proliferator-activated receptor gamma and fatty acid transferase expressions.

  2009cites this paper
• Regulation of GLUT4 intrinsic activity and internalization in L6 muscle cells.

  2009cites this paper
• Insulin-stimulated translocation of glucose transporter (GLUT) 12 parallels that of GLUT4 in normal muscle.

  2009cites this paper
• Effects of ketamine on glucose uptake by glucose transporter type 3 expressed in Xenopus oocytes: The role of protein kinase C.

  2009cites this paper
• Stimulation of muscle cell glucose uptake by resveratrol through sirtuins and AMPK.

  2008cites this paper
• Early pregnancy maternal endocrine insulin-like growth factor I programs the placenta for increased functional capacity throughout gestation.

  2007cites this paper
• Early treatment of the pregnant guinea pig with IGFs promotes placental transport and nutrient partitioning near term.

  2007cites this paper
• GLUCOSAMINE REDUCES GLYCOGEN STORAGE IN L6 SKELETAL MUSCLE CELLS

  2007cites this paper
• IGF-1 controls GLUT3 expression in muscle via the transcriptional factor Sp1.

  2007cites this paper
• Neonatal Nutrition and Metabolism: Fetal nutrition

  2006cites this paper
• Metabolic effects of insulin and IGFs on Gilthead Sea Bream (Sparus aurata) muscle cultured cells

  2006cites this paper
• Insulin regulates neuronal glucose uptake by promoting translocation of glucose transporter GLUT3.

  2006cites this paper
• Hexose transporter mRNAs for GLUT4, GLUT5, and GLUT12 predominate in human muscle.

  2006cites this paper
• Reduction of insulin-stimulated glucose uptake in L6 myotubes by the protein kinase inhibitor SB203580 is independent of p38MAPK activity.

  2005cites this paper
• Transportadores de glucosa en el embarazo: Revisión

  2005cites this paper
• Insulin-like Growth Factor Binding Protein-1 and Interleukin-6 are Markers of Fetal Stress During Parturition at Term Gestation

  2005cites this paper
• Thyroid Hormone Excess Increases Basal and Insulin-stimulated Recruitment of GLUT3 Glucose Transporters on Cell Surface

  2005cites this paper
• Desperately seeking sugar: glial cells as hypoglycemia sensors.

  2005cites this paper
• Evaluation of glucose transport and its regulation by insulin in human monocytes using flow cytometry

  2005influential citation
• C2C12 Skeletal Muscle Cells Exposure to Phosphatidylcholine Triggers IGF-1 Like-Responses

  2005cites this paper
• Acute regulation of Glut1 function : the role of detergent-resistant membrane domains.

  2004cites this paper
• Disruption of Cortical Actin in Skeletal Muscle Demonstrates an Essential Role of the Cytoskeleton in Glucose Transporter 4 Translocation in Insulin-sensitive Tissues*

  2004cites this paper
• The Effects of Insulin and Metformin on Glucose Uptake in L 8 Myotubes

  2004cites this paper
• Neuronal protection from glucose deprivation via modulation of glucose transport and inhibition of apoptosis: a role for the insulin-like growth factor system.

  2004cites this paper
• The Effects of Insulin and Metformin on Glucose Uptake in L8 Myotubes

  2003cites this paper
• Exercise- and insulin-stimulated muscle glucose transport: distinct mechanisms of regulation.

  2002cites this paper
• Sustained exposure of L6 myotubes to high glucose and insulin decreases insulin-stimulated GLUT4 translocation but upregulates GLUT4 activity.

  2002cites this paper
• Glucose transport and metabolism in chondrocytes: a key to understanding chondrogenesis, skeletal development and cartilage degradation in osteoarthritis.

  2002cites this paper
• Insulin-like growth factor 2 and potential regulators of hemangioma growth and involution identified by large-scale expression analysis

  2002cites this paper
• Placental glucose transfer and fetal growth

  2002cites this paper
• C2C12 myocytes lack an insulin-responsive vesicular compartment despite dexamethasone-induced GLUT4 expression.

  2002cites this paper
• C 2 C 12 myocytes lack an insulin-responsive vesicular compartment despite dexamethasone-induced GLUT 4 expression

  2002cites this paper
• Volumes 357–377 (1995)

  2001cites this paper
• Specificity in ligand binding and intracellular signalling by insulin and insulin-like growth factor receptors.

  2001cites this paper
• Altered GLUT1 and GLUT3 gene expression and subcellular redistribution of GLUT4: protein in muscle from patients with acanthosis nigricans and severe insulin resistance.

  2001cites this paper
• Regulation of Glucose Transporters by Insulin and Exercise: Cellular Effects and Implications for Diabetes

  2001cites this paper
• Positron-emission tomography with [18F]fluorodeoxyglucose

  2000cites this paper
• Reduced GLUT1 expression in Igf1-/- null oocytes and follicles.

  2000cites this paper
• Placental glucose transport in diabetic pregnancy.

  2000cites this paper
• Comparison of GLUT1, GLUT3, and GLUT4 mRNA and the subcellular distribution of their proteins in normal human muscle.

  2000cites this paper
• Differential Role of Insulin Receptor Substrate (IRS)-1 and IRS-2 in L6 Skeletal Muscle Cells Expressing the Arg1152 → Gln Insulin Receptor*

  1999cites this paper
• Igf1 promotes longitudinal bone growth by insulin‐like actions augmenting chondrocyte hypertrophy

  1999influential citation
• Diabetes alters the expression and activity of the human placental GLUT1 glucose transporter.

  1999cites this paper
• Mobilization of GLUT-4 from intracellular vesicles by insulin and K+ depolarization in cultured H9c2 myotubes.

  1999cites this paper
• Glucose transporter 3 (GLUT3) protein is present in human myocardium.

  1999cites this paper
• Mobilization of GLUT4 from intracellular vesicles by insulin and K 1 depolarization in cultured H 9 c 2 myotubes

  1999cites this paper
• Tyrosine phosphorylation of specific protein kinase C isoenzymes participates in insulin stimulation of glucose transport in primary cultures of rat skeletal muscle.

  1999cites this paper
• IGF-1 induces skeletal myocyte hypertrophy through calcineurin in association with GATA-2 and NF-ATc1

  1999cites this paper
• The insulin-sensitive glucose transporter (GLUT4) is involved in early bone growth in control and diabetic mice, but is regulated through the insulin-like growth factor I receptor.

  1999cites this paper
• Insulin-like growth factor type 1—friend or foe?

  1998cites this paper
• GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay

  1998cites this paper
• Pulsatile ocular blood flow

  1998cites this paper
• Unique mechanism of GLUT3 glucose transporter regulation by prolonged energy demand: increased protein half-life.

  1998cites this paper
• Effects of [beta]-amyloid on glucose uptake by cultured hippocampal neurons

  1998cites this paper
• Glycaemic regulation of glucose transporter expression and activity in the human placenta.

  1998cites this paper
• Quantitative methods for measuring the insulin-regulatable glucose transporter (Glut4).

  1997cites this paper
• Glucose transporters of the glomerulus and the implications for diabetic nephropathy.

  1997cites this paper
• D-Glucose Stimulates Mesangial Cell GLUT1 Expression and Basal and IGF-I–Sensitive Glucose Uptake in Rat Mesangial Cells: Implications for Diabetic Nephropathy

  1997cites this paper
• Thrombin-induced translocation of GLUT3 glucose transporters in human platelets.

  1997cites this paper
• Cell biology of insulin action on glucose transport.

  1997cites this paper
• Insulin regulation and selective segregation with glucose transporter-4 of the membrane aminopeptidase vp165 in rat skeletal muscle cells.

  1997cites this paper
• Stimulation of Glucose Uptake by the Natural Coenzyme α-Lipoic Acid/Thioctic Acid: Participation of Elements of the Insulin Signaling Pathway

  1996cites this paper
• Dexamethasone modulates insulin receptor expression and subcellular distribution of the glucose transporter GLUT 1 in UMR 106-01, a clonal osteogenic sarcoma cell line.

  1996cites this paper
• Expression and regulation by insulin of GLUT 3 in UMR 106-01, a clonal rat osteosarcoma cell line.

  1996cites this paper
• Thyroid hormone inhibition in L6 myoblasts of IGF-I-mediated glucose uptake and proliferation: new roles for integrin (cid:2) v (cid:3) 3

  year unknowncites this paper