3D-Bioreactor culture of human hepatoma cell line HepG2 as a promising tool for in vitro substance testing

Future developments in pharmaceutical research and regulatory requirements such as the European REACH program require high numbers of animal experiments. As a result of ethical concerns, cell culture tests with human cell lines or primary cells are considered as an alternative. However, current testing protocols using 2D cell cultures in Petri dishes are not equivalent to animal trials. 3D tissue cultures may overcome fundamental obstacles in the development of new therapeutic agents. Many new candidates of therapeutic agents are intended as agonists or antagonists of specific receptors on human cells. For these substances, organ-like test systems based on human cells are mandatory. In some cases, new pharmaceuticals lead to unexpected adverse reactions even after successful animal trials. It is assumed that 3D test systems based on human cells might help to overcome these problems.

• Publication year

  2011

• Venue

  BMC Proceedings

• Publication date

  2011-11-22

• Fields of study

  Medicine, Engineering

• Identifiers
  DOI 10.1186/1753-6561-5-S8-P61PMID 22373268PMCID 3284898
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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