Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate.

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Biochemistry

• Publication date

  2007-10-03

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI700844MPMID 17914867
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Crystallographic snapshots of oxalyl-CoA decarboxylase give insights into catalysis by nonoxidative ThDP-dependent decarboxylases.

  2007cited by this paper
• A versatile conformational switch regulates reactivity in human branched-chain alpha-ketoacid dehydrogenase.

  2006cited by this paper
• Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate.

  2006cited by this paper
• Binding energy, specificity, and enzymic catalysis: the circe effect.

  2006cited by this paper
• A Thiamin-bound, Pre-decarboxylation Reaction Intermediate Analogue in the Pyruvate Dehydrogenase E1 Subunit Induces Large Scale Disorder-to-Order Transformations in the Enzyme and Reveals Novel Structural Features in the Covalently Bound Adduct*

  2006cited by this paper
• The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography

  2006cited by this paper
• Coot: model-building tools for molecular graphics.

  2004cited by this paper
• A Molecular Switch and Proton Wire Synchronize the Active Sites in Thiamine Enzymes

  2004cited by this paper
• How Enzymes Work: Analysis by Modern Rate Theory and Computer Simulations

  2004cited by this paper
• Tetrahedral intermediates in thiamin diphosphate-dependent decarboxylations exist as a 1',4'-imino tautomeric form of the coenzyme, unlike the michaelis complex or the free coenzyme.

  2004cited by this paper
• Ligand-induced conformational changes and a reaction intermediate in branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8, as revealed by X-ray crystallography.

  2004cited by this paper
• A Perspective on Enzyme Catalysis

  2003cited by this paper
• NMR analysis of covalent intermediates in thiamin diphosphate enzymes.

  2003cited by this paper
• The near attack conformation approach to the study of the chorismate to prephenate reaction

  2003cited by this paper
• Current mechanistic understanding of thiamin diphosphate-dependent enzymatic reactions.

  2003cited by this paper
• Snapshot of a key intermediate in enzymatic thiamin catalysis: Crystal structure of the α-carbanion of (α,β-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae

  2002cited by this paper
• Examination of Donor Substrate Conversion in Yeast Transketolase*

  2001cited by this paper
• The importance of reactant positioning in enzyme catalysis: a hybrid quantum mechanics/molecular mechanics study of a haloalkane dehalogenase.

  2000cited by this paper
• Electrostatic stress in catalysis: structure and mechanism of the enzyme orotidine monophosphate decarboxylase.

  2000cited by this paper
• Properties and functions of the thiamin diphosphate dependent enzyme transketolase.

  1998cited by this paper
• Asp477 is a determinant of the enantioselectivity in yeast transketolase

  1998cited by this paper
• Crystallography and mutagenesis of transketolase: mechanistic implications for enzymatic thiamin catalysis.

  1998cited by this paper
• Sixty years of thiamin diphosphate biochemistry.

  1998cited by this paper
• Identification of catalytically important residues in yeast transketolase.

  1997cited by this paper
• Examination of Substrate Binding in Thiamin Diphosphate- dependent Transketolase by Protein Crystallography and Site-directed Mutagenesis*

  1997cited by this paper
• His103 in yeast transketolase is required for substrate recognition and catalysis.

  1995cited by this paper
• Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains.

  1995cited by this paper
• Analysis of an invariant cofactor-protein interaction in thiamin diphosphate-dependent enzymes by site-directed mutagenesis. Glutamic acid 418 in transketolase is essential for catalysis.

  1994cited by this paper
• Furanose ring anomerization: kinetic and thermodynamic studies of the D-2-pentuloses by 13C-n.m.r. spectroscopy.

  1990cited by this paper
• LOW-LYING PI-ELECTRON STATES OF TRANS-BUTADIENE

  1973cited by this paper
• Proportion of keto and aldehydo forms in solutions of sugars and sugar phosphates.

  1971cited by this paper

• Biophysical and structural studies on transketolases.

  2025cites this paper
• 1-Deoxy-D-xylulose 5-phosphate synthase: structural perspectives on an essential enzyme in isoprenoid biosynthesis.

  2025cites this paper
• Cell-Free Reaction System for ATP Regeneration from d-Fructose

  2025cites this paper
• Biochemical, Bioinformatic, and Structural Comparisons of Transketolases and Position of Human Transketolase in the Enzyme Evolution.

  2024cites this paper
• Disruption of an Active Site Network Leads to Activation of C2α-Lactylthiamin Diphosphate on the Antibacterial Target 1-Deoxy-d-xylulose-5-phosphate Synthase.

  2024cites this paper
• Aldehyde‐based Activation of C2α‐lactylthiamin Diphosphate Decarboxylation on Bacterial 1‐deoxy‐d‐xylulose 5‐phosphate Synthase

  2024cites this paper
• Multifaceted Role of the Substrate Phosphate Group in Transketolase Catalysis

  2023cites this paper
• New Role of Water in Transketolase Catalysis

  2023cites this paper
• Extein residues regulate the catalytic function of Spl DnaX intein enzyme by restricting the near‐attack conformations of the active‐site residues

  2023cites this paper
• Ambient temperature structure of phosphoketolase from Bifidobacterium longum determined by serial femtosecond X-ray crystallography.

  2023cites this paper
• Methanol-Dependent Carbon Fixation for Irreversible Synthesis of d-Allulose from d-Xylose by Engineered Escherichia coli.

  2022cites this paper
• Ground-state destabilization by electrostatic repulsion is not a driving force in orotidine-5′-monophosphate decarboxylase catalysis

  2022cites this paper
• Engineering of enzymes using non-natural amino acids

  2022cites this paper
• Antibacterial Target DXP Synthase Catalyzes the Cleavage of d-Xylulose 5-Phosphate: a Study of Ketose Phosphate Binding and Ketol Transfer Reaction.

  2022cites this paper
• Frontiers in the enzymology of thiamin diphosphate-dependent enzymes.

  2022cites this paper
• Structural and biochemical characterizations of Thermus thermophilus HB8 transketolase producing a heptulose

  2022cites this paper
• High-resolution structure of phosphoketolase from Bifidobacterium longum determined by cryo-EM single-particle analysis.

  2022cites this paper
• Regulation of enzymes with identical subunits on the example of Transketolase

  2022cites this paper
• Characterisation of a hyperthermophilic transketolase from Thermotoga maritima DSM3109 as a biocatalyst for 7-keto-octuronic acid synthesis

  2021cites this paper
• Directing cation-cation interactions in thiamine compounds: Analysis of a series of organic salts based on vitamin B1

  2021cites this paper
• Mechanistically Diverse Pathways for Sulfoquinovose Degradation in Bacteria

  2021cites this paper
• Water Molecules Allow the Intramolecular Activation of the Thiamine Di-Phosphate Cofactor in Human Transketolase: Mechanistic Insights into a Famous Proposal

  2021cites this paper
• The mechanism of a one-substrate transketolase reaction

  2020cites this paper
• Assessing the Thiamine Diphosphate Dependent Pyruvate Dehydrogenase E1 Subunit for Carboligation Reactions with Aliphatic Ketoacids

  2020cites this paper
• How the Destabilization of a Reaction Intermediate Affects Enzymatic Efficiency: The Case of Human Transketolase

  2020cites this paper
• Structural basis for antibiotic action of the B1 antivitamin 2′-methoxy-thiamine

  2020cites this paper
• Simultaneous optimization of donor and acceptor substrate specificity for transketolase by a small but smart library

  2019cites this paper
• Evolved Thermostable Transketolase for Stereoselective Two-Carbon Elongation of Non-Phosphorylated Aldoses to Naturally Rare Ketoses

  2019cites this paper
• Crystal structure of a xylulose 5-phosphate phosphoketolase. Insights into the substrate specificity for xylulose 5-phosphate.

  2019cites this paper
• The Catalytic Mechanism of Human Transketolase.

  2019cites this paper
• Engineering transketolase to accept both unnatural donor and acceptor substrates and produce α‐hydroxyketones

  2019cites this paper
• Low-barrier hydrogen bonds in enzyme cooperativity

  2019cites this paper
• Thermodynamically and Kinetically Controlled Reactions in Biocatalysis – from Concepts to Perspectives

  2019cites this paper
• Protein engineering of transketolase for industrial applications

  2019influential citation
• Novel insights into transketolase activation by cofactor binding identifies two native species subpopulations

  2019cites this paper
• Phosphoketolase - A mechanistic update

  2018cites this paper
• Evidence of Diradicals Involved in the Yeast Transketolase Catalyzed Keto‐Transferring Reactions

  2018cites this paper
• The Mesomeric Effect of Thiazolium on non-Kekulé Diradicals in Pichia stipitis Transketolase.

  2018cites this paper
• Single‐Turnover Kinetics Reveal a Distinct Mode of Thiamine Diphosphate‐Dependent Catalysis in Vitamin K Biosynthesis

  2018cites this paper
• Novel Insights in Structure and Mechanism of Escherichia coli Transketolase

  2018influential citation
• Towards a Mechanistic Understanding of Factors Controlling the Stereoselectivity of Transketolase

  2018cites this paper
• Transketolase A from E. coli Significantly Suppresses Protein Glycation by Glycolaldehyde and Glyoxal in Vitro.

  2017cites this paper
• Engineering a thermostable transketolase for arylated substrates

  2017cites this paper
• Donor Promiscuity of a Thermostable Transketolase by Directed Evolution: Efficient Complementation of 1-Deoxy-d-xylulose-5-phosphate Synthase Activity.

  2017cites this paper
• Phage Display on the Anti‐infective Target 1‐Deoxy‐d‐xylulose‐5‐phosphate Synthase Leads to an Acceptor–Substrate Competitive Peptidic Inhibitor

  2017cites this paper
• Ingénierie de la transcétolase de Geobacillus stearothermophilus : nouvelles stratégies pour la synthèse enzymatique de cetoses rares

  2017cites this paper
• Theoretical study of the catalytic mechanism of glyoxylate carboligase and its mutant V51E

  2017cites this paper
• Donor‐Promiskuität einer thermostabilen Transketolase durch gelenkte Evolution – effektive Komplementierung der 1‐Desoxy‐d‐ xylulose‐5‐phosphat‐Synthase‐Aktivität

  2017cites this paper
• Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose

  2016cites this paper
• Insights into the Thiamine Diphosphate Enzyme Activation Mechanism: Computational Model for Transketolase Using a Quantum Mechanical/Molecular Mechanical Method.

  2016cites this paper
• Structural and biochemical interrogation on transketolase from Pichia stipitis for new functionality.

  2016cites this paper
• Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes

  2016cites this paper
• Second generation engineering of transketolase for polar aromatic aldehyde substrates.

  2015cites this paper
• Substrate Distortion and the Catalytic Reaction Mechanism of 5-Carboxyvanillate Decarboxylase

  2015cites this paper
• Systematic analysis of the sequence-structure-function relationships of thiamine diphosphate-dependent enzymes

  2015cites this paper
• Efficient production of L-lactic acid by an engineered Thermoanaerobacterium with

  2015influential citation
• A thermostable transketolase evolved for aliphatic aldehyde acceptors.

  2015cites this paper
• A dual conformation of the post-decarboxylation intermediate is associated with distinct enzyme states in mycobacterial KGD (α-ketoglutarate decarboxylase).

  2014cites this paper
• Structure and functioning mechanism of transketolase.

  2014cites this paper
• Sweet siblings with different faces: the mechanisms of FBP and F6P aldolase, transaldolase, transketolase and phosphoketolase revisited in light of recent structural data.

  2014cites this paper
• Etude de la transcétolase de Geobacillus stearothermophilus et modification de son énantiosélectivité par ingénierie enzymatique

  2014cites this paper
• Marvels of enzyme catalysis at true atomic resolution: distortions, bond elongations, hidden flips, protonation states and atom identities.

  2014cites this paper
• Identification of major zinc-binding proteins from a marine cyanobacterium: insight into metal uptake in oligotrophic environments.

  2014cites this paper
• Functional discovery and promiscuity in the RuBisCO superfamily

  2013influential citation
• Theoretical studies on the common catalytic mechanism of transketolase by using simplified models.

  2013cites this paper
• CC bond formation using ThDP-dependent lyases.

  2013cites this paper
• Theoretical study of the catalytic mechanism of E1 subunit of pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus.

  2013cites this paper
• Stabilizing biocatalysts.

  2013cites this paper
• Sub-ångström-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate.

  2013cites this paper
• Alternating sites reactivity is a common feature of thiamin diphosphate-dependent enzymes as evidenced by isothermal titration calorimetry studies of substrate binding.

  2013cites this paper
• Engineering stereoselectivity of ThDP‐dependent enzymes

  2013cites this paper
• Computational studies on the catalytic mechanism of phosphoketolase

  2013cites this paper
• Using site‐saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate‐dependent enzymes

  2013cites this paper
• Enzymatic Properties and Directed Evolution of Transketolase and Chemoenzymatic Synthesis of neo-Sialoconjugates

  2012influential citation
• A Δ38 Deletion Variant of Human Transketolase as a Model of Transketolase-Like Protein 1 Exhibits No Enzymatic Activity

  2012cites this paper
• 7.17 C-X Bond Formation: C-C Bond Formation using TDP-Dependent Enzymes

  2012cites this paper
• Acyloin and Benzoin Condensations

  2012cites this paper
• enzyme and comparison with the human Mycobacterium tuberculosis Structure and function of the transketolase from

  2012cites this paper
• Diphenyl Urea Derivatives as Inhibitors of Transketolase: A Structure-Based Virtual Screening

  2012cites this paper
• Rational substrate and enzyme engineering of transketolase for aromatics.

  2012cites this paper
• Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme

  2012cites this paper
• A preliminary X-ray study of transketolase from Burkholderia pseudomallei.

  2012cites this paper
• heoretical studies on the common catalytic mechanism of transketolase by sing simplified models

  2012cites this paper
• Twisted Schiff base intermediates and substrate locale revise transaldolase mechanism.

  2011cites this paper
• Thiamin diphosphate catalysis in Escherichia coli pyruvate dehydrogenase multi-enzyme complex: Activation, covalent electrophilic catalysis, and substrate channeling

  2011cites this paper
• Significant catalytic roles for Glu47 and Gln 110 in all four of the C-C bond-making and -breaking steps of the reactions of acetohydroxyacid synthase II.

  2011cites this paper
• Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum.

  2011cites this paper
• C-C bond-forming lyases in organic synthesis.

  2011cites this paper
• Transketolase in Trypanosoma brucei.

  2011cites this paper
• The Crystal Structure of Human Transketolase and New Insights into Its Mode of Action*

  2010cites this paper
• Substrate Conformations Set the Rate of Enzymatic Acrylation by Lipases

  2010cites this paper
• Double duty for a conserved glutamate in pyruvate decarboxylase: evidence of the participation in stereoelectronically controlled decarboxylation and in protonation of the nascent carbanion/enamine intermediate .

  2010cites this paper
• Crystal Structures of Phosphoketolase

  2010cites this paper
• Identification of potential P. falciparum transketolase inhibitors: pharmacophore design, in silico screening and docking studies

  2010cites this paper
• Valine 375 and phenylalanine 109 confer affinity and specificity for pyruvate as donor substrate in acetohydroxy acid synthase isozyme II from Escherichia coli.

  2010cites this paper
• Crystal structure of Bifidobacterium Longum phosphoketolase; key enzyme for glucose metabolism in Bifidobacterium

  2010cites this paper
• Structural Insights into the Prereaction State of Pyruvate Decarboxylase from Zymomonas mobilis†,‡

  2010cites this paper
• Non-alpha-hydroxylated aldehydes with evolved transketolase enzymes.

  2010cites this paper
• Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

  2010cites this paper
• Detection and time course of formation of major thiamin diphosphate-bound covalent intermediates derived from a chromophoric substrate analogue on benzoylformate decarboxylase.

  2009cites this paper