Pretreatment of normal human peripheral blood lymphocytes with the plant lectin, concanavalin A (Con A), results in inhibition of blast transformation and [3H]thymidine incorporation by untreated allogeneic lymphocytes from healthy volunteers donors in one-way mixed leukocyte culture. Similarly, responses to mitogens, certain microbial antigens, and allogeneic lymphocytes are inhibited by Con A-treated allogeneic cells. Con A pretreated autologous lymphocytes can also be induced to manifest suppressor activities. This antimitotic effect occurs without evidence of cytotoxicity and is active on de novo lymphocyte responses and does not require prior sensitization of the cells being tested. Suppression of the lymphocyte response to pokeweed mitogen, a potent B- cell stimulator, by Con A-pretreated suppressor cells was not as consistent as was inhibition of response to other mitogens, including phytohemagglutinin and Con A. Furthermore, suppression of lymphocyte transformation to the microbial antigens, tuberculin purified protein derivative, and Canadida albicans extracts could be similarly induced by Con A pretreatment of either allogeneic or autologous cells. Induction of autologous suppressor activity in lymphocytes from healthy donors is compatible with a model that includes a role for suppressor cells in the modulation of the normal immune response.
Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors
Published 1976 in Journal of Experimental Medicine
ABSTRACT
PUBLICATION RECORD
- Publication year
1976
- Venue
Journal of Experimental Medicine
- Publication date
1976-05-01
- Fields of study
Biology, Medicine
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- allogeneic lymphocytes
Lymphocytes obtained from a genetically different donor used as responder or stimulator cells.
Aliases: allogeneic cells
- autologous lymphocytes
Lymphocytes from the same donor used as the source of responder cells.
Aliases: autologous cells
- blast transformation
The morphological and proliferative conversion of resting lymphocytes into activated blast cells.
Aliases: lymphocyte blast transformation
- concanavalin a treatment
Pretreatment of lymphocytes with the plant lectin concanavalin A before functional assay.
Aliases: Con A treatment, Con A pretreatment, Con A
- microbial antigens
Antigen preparations derived from microorganisms and used to test lymphocyte responsiveness.
Aliases: microbial antigen extracts
- mitogens
Substances that stimulate lymphocyte activation and proliferation in culture.
Aliases: lymphocyte mitogens
- one-way mixed leukocyte culture
A mixed-cell assay in which lymphocytes from one donor are tested against allogeneic stimulating cells.
Aliases: one-way MLC, mixed leukocyte culture
- phytohemagglutinin
A plant lectin commonly used as a T-cell mitogen in lymphocyte assays.
Aliases: PHA
- pokeweed mitogen
A plant-derived mitogen used to stimulate lymphocyte proliferation, especially B-cell responses.
Aliases: PWM
- suppressor cell activity
The capacity of treated lymphocytes to inhibit other lymphocyte responses in functional assays.
Aliases: suppressor activity
- thymidine incorporation
Uptake of radiolabeled thymidine used to measure DNA synthesis during lymphocyte proliferation.
Aliases: [3H]thymidine incorporation, tritiated thymidine incorporation
REFERENCES
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