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The isolation and characterization of adenosine monophosphate-rich polynucleotides synthesized by Ehrlich ascites cells.

M. Edmonds,M. G. Caramela

Published 1969 in Journal of Biological Chemistry

ABSTRACT

Abstract Polynucleotides containing AMP in excess of 90 mole % account for almost 1% of the total RNA synthesized by Ehrlich ascites cells growing either in the mouse or during short incubations in vitro. A method based on the affinity of these AMP-rich polynucleotides for polythymidylate oligomers covalently bound to cellulose has been used to isolate them from the total cellular RNA. The most highly purified fractions are relatively large polynucleotides of the order of 8 to 10 S as judged by their electrophoretic mobility on polyacrylamide gels relative to that of a number of well characterized RNA molecules. They are located primarily in the cell nucleus, for almost 90% of the polyadenylate of whole cells was recovered in nuclei from an aqueous homogenate of these cells. The suppression of polyadenylate synthesis by relatively low doses of actinomycin D suggests that it may be synthesized on a DNA template.

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