The determination of lactic acid.

Lactic acid is a substance of major significance throughout the orders of life, from microbe to man. Its relation to the carbohydrate metabolism has been very extensively st,udied; and it is not surprising, therefore, that many methods have been proposed for its quantitative estimation. Most of the methods depend upon the conversion of lactic acid into acetaldehyde (1, 2), the latter being determined as such, or calorimetrically after reacting with some color-producing substance. Of these methods, perhaps the most precise is that of Friedemann, Cotonio, and Shaffer (3,4). Lactic acid, according to this method, is oxidized by acid KMn04 in the presence of MnS04. The resulting acetaldehyde is aerated out of the solution, absorbed in bisulfite, and determined by the Clausen (2) titration method. The chief advantages of the method over the older oxidation methods of von Fiirth and Charnas and Clausen are (1) its speed, (2) the considerably increased yield of acetaldehyde, and (3) the smaller fluctuations between individual determinations. This method, as in the case of the older methods, determines also the more volatile sulfitebinding substances which may be produced from many other compounds besides lactic acid. Their effect on the determination may be minimized, however, as was shown by Friedemann, Cotonio, and Shaffer, merely by allowing the vapors to pass upward through a cooled reflux condenser, the less volatile substances being condensed with the steam and returned to the solution where apparently they are destroyed by further oxidation.

• Publication year

  1933

• Venue

  Journal of Biological Chemistry

• Publication date

  1933-03-01

• Fields of study

  Biology, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)63684-1
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• No references are available for this paper.

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