Catalytic independent functions of a protein kinase as revealed by a kinase-dead mutant: study of the Lys72His mutant of cAMP-dependent kinase.

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Journal of Molecular Biology

• Publication date

  2005-09-02

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/J.JMB.2005.06.011PMID 16054648
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Consequences of Lysine 72 Mutation on the Phosphorylation and Activation State of cAMP-dependent Kinase*

  2005cited by this paper
• Allosteric network of cAMP-dependent protein kinase revealed by mutation of Tyr204 in the P+1 loop.

  2005cited by this paper
• Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS.

  2004cited by this paper
• EphB1-mediated Cell Migration Requires the Phosphorylation of Paxillin at Tyr-31/Tyr-118*

  2004influential reference
• Casein Kinase Iϵ Plays a Functional Role in the Transforming Growth Factor-β Signaling Pathway*

  2004cited by this paper
• Casein kinase Iepsilon plays a functional role in the transforming growth factor-beta signaling pathway.

  2004influential reference
• Protein analysis by hydrogen exchange mass spectrometry.

  2003cited by this paper
• Dynamics of cAPK type IIbeta activation revealed by enhanced amide H/2H exchange mass spectrometry (DXMS).

  2003cited by this paper
• A dominant negative mutant of TLK1 causes chromosome missegregation and aneuploidy in normal breast epithelial cells

  2003influential reference
• Identification of the protein kinase A regulatory RIα-catalytic subunit interface by amide H/2H exchange and protein docking

  2003cited by this paper
• Protein structure change studied by hydrogen-deuterium exchange, functional labeling, and mass spectrometry

  2003cited by this paper
• Dynamic features of cAMP-dependent protein kinase revealed by apoenzyme crystal structure.

  2003cited by this paper
• The Protein Kinase Complement of the Human Genome

  2002influential reference
• Phosphorylation of the Catalytic Subunit of Protein Kinase A

  2002cited by this paper
• Dynamics of cAMP-dependent protein kinase.

  2001cited by this paper
• Dynamics of cAMP-Dependent Protein Kinase

  2001cited by this paper
• Structural Characterization of Protein Kinase A as a Function of Nucleotide Binding

  2001cited by this paper
• High resolution, high‐throughput amide deuterium exchange‐mass spectrometry (DXMS) determination of protein binding site structure and dynamics: Utility in pharmaceutical design

  2001cited by this paper
• Changes in protein conformational mobility upon activation of extracellular regulated protein kinase-2 as detected by hydrogen exchange.

  2001cited by this paper
• Insights into nucleotide binding in protein kinase A using fluorescent adenosine derivatives

  2000cited by this paper
• The 2.35 A crystal structure of the inactivated form of chicken Src: a dynamic molecule with multiple regulatory interactions.

  1997cited by this paper
• Crystal structure of the Src family tyrosine kinase Hck

  1997cited by this paper
• Mutation of position 52 in ERK2 creates a nonproductive binding mode for adenosine 5'-triphosphate.

  1996cited by this paper
• Active site mutations define the pathway for the cooperative activation of cAMP-dependent protein kinase.

  1996cited by this paper
• The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification 1

  1995cited by this paper
• Phosphorylation modulates catalytic function and regulation in the cAMP-dependent protein kinase.

  1995cited by this paper
• Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex

  1995cited by this paper
• Crosstalk between domains in the regulatory subunit of cAMP-dependent protein kinase: influence of amino terminus on cAMP binding and holoenzyme formation.

  1994cited by this paper
• The conserved lysine of the catalytic domain of protein kinases is actively involved in the phosphotransfer reaction and not required for anchoring ATP.

  1993cited by this paper
• Crystal structure of cyclin-dependent kinase 2

  1993cited by this paper
• Determination of amide hydrogen exchange by mass spectrometry: A new tool for protein structure elucidation

  1993cited by this paper
• Systematic mutational analysis of cAMP-dependent protein kinase identifies unregulated catalytic subunits and defines regions important for the recognition of the regulatory subunit.

  1992cited by this paper
• Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase.

  1991cited by this paper
• A kinase-negative mutant of S49 mouse lymphoma cells is defective in posttranslational maturation of catalytic subunit of cyclic AMP-dependent protein kinase

  1991influential reference
• Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions.

  1991cited by this paper
• Dicyclohexylcarbodiimide cross-links two conserved residues, Asp-184 and Lys-72, at the active site of the catalytic subunit of cAMP-dependent protein kinase.

  1989cited by this paper
• Differential labeling of the catalytic subunit of cAMP-dependent protein kinase with acetic anhydride: substrate-induced conformational changes.

  1989cited by this paper
• The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.

  1988cited by this paper
• Determination and analysis of urea and guanidine hydrochloride denaturation curves.

  1986cited by this paper
• Evaluation of the intramolecular stacking of the fluorosulfonylbenzoyl derivatives of 1,N6-ethenoadenosine, adenosine, and guanosine.

  1984cited by this paper
• Direct evidence that oncogenic tyrosine kinases and cyclic AMP-dependent protein kinase have homologous ATP-binding sites

  1984cited by this paper
• Adenosine cyclic 3',5'-monophosphate dependent protein kinase: nucleotide binding to the chemically modified catalytic subunit.

  1984cited by this paper
• Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71.

  1981cited by this paper
• Primary structure effects on peptide group hydrogen exchange.

  1972cited by this paper
• Conversion of phosphorylase b to phosphorylase a in muscle extracts.

  1955cited by this paper

• C-spine mutations of protein kinase C and Akt as a novel generalizable approach to create stable pseudokinases

  2025cites this paper
• Advancing Covalent Ligand and Drug Discovery beyond Cysteine

  2025cites this paper
• Phosphorylation of a conserved intrinsically disordered region is necessary for activation of a bacterial Hanks-type Ser/Thr kinase signaling pathway

  2025cites this paper
• Emerging and Re-emerging Warheads for Targeted Covalent Inhibitors: An Update.

  2024cites this paper
• The RhoGAP RRC-1 is required for the assembly or stability of integrin adhesion complexes and is a member of the PIX pathway in muscle

  2023cites this paper
• Fast Identification of In Vivo Protein Phosphorylation Events Using Transient Expression in Leaf Mesophyll Protoplasts and Phos-tagTM SDS-PAGE.

  2023cites this paper
• CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASES - their evolution, structure and roles in stress response and development.

  2023cites this paper
• The "violin model": Looking at community networks for dynamic allostery.

  2023cites this paper
• ASK1-K716R reduces neuroinflammation and white matter injury via preserving blood–brain barrier integrity after traumatic brain injury

  2023cites this paper
• Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite

  2023cites this paper
• A critical evaluation of protein kinase regulation by activation loop autophosphorylation

  2023cites this paper
• The Rho GAP RRC-1 is a member of the PIX pathway and controls assembly or stability of integrin adhesion complexes in muscle

  2022cites this paper
• Fluorescent Translocation Reporters for Sub-plasma Membrane cAMP Imaging.

  2022cites this paper
• Yeast galactokinase in closed conformation can switch between catalytic and signal transducer states

  2022cites this paper
• Conformational control and regulation of the pseudokinase KSR via small molecule binding interactions.

  2022cites this paper
• Pseudokinases: Prospects for expanding the therapeutic targets armamentarium.

  2021cites this paper
• PKA Cα subunit mutation triggers caspase-dependent RIIβ subunit degradation via Ser114 phosphorylation

  2021influential citation
• Synergistic Allostery in Multiligand-Protein Interactions

  2020cites this paper
• Phosphoproteomics Meets Chemical Genetics: Approaches for Global Mapping and Deciphering the Phosphoproteome

  2020cites this paper
• Acetylation of the Catalytic Lysine Inhibits Kinase Activity in PI3Kδ.

  2020cites this paper
• The cysteine-rich receptor-like kinase CRK2 during stress responses in Arabidopsis thaliana

  2019cites this paper
• Cryo-EM Structure of Nucleotide-Bound Tel1ATM Unravels the Molecular Basis of Inhibition and Structural Rationale for Disease-Associated Mutations

  2019cites this paper
• Structure of nucleotide-bound Tel1ATM reveals the molecular basis of inhibition and structural rationale for disease mutations

  2019cites this paper
• Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification

  2019cites this paper
• The pseudokinase domains of guanylyl cyclase–A and –B allosterically increase the affinity of their catalytic domains for substrate

  2019cites this paper
• Expression of the Conserved Herpesvirus Protein Kinase (CHPK) of Marek’s Disease Alphaherpesvirus in the Skin Reveals a Mechanistic Importance for CHPK during Interindividual Spread in Chickens

  2019cites this paper
• The Role of Post-Translational Modifications and Allostery in the Receptor Guanylyl Cyclases GC-A and GC-B

  2018cites this paper
• Twitchin kinase inhibits muscle activity

  2017influential citation
• The Role of the UNC-82 Protein Kinase in Organizing Myosin Filaments in Striated Muscle of Caenorhabditis elegans

  2016cites this paper
• Protein kinase A can block EphA2 receptor–mediated cell repulsion by increasing EphA2 S897 phosphorylation

  2016influential citation
• Variants of the yeast MAPK Mpk1 are fully functional independently of activation loop phosphorylation

  2016cites this paper
• Deacetylation by SIRT3 Relieves Inhibition of Mitochondrial Protein Function

  2016cites this paper
• cAMP-dependent protein kinase (PKA) complexes probed by complementary differential scanning fluorimetry and ion mobility–mass spectrometry

  2016cites this paper
• Decoding the Interactions Regulating the Active State Mechanics of Eukaryotic Protein Kinases

  2016cites this paper
• ATP binding to the pseudokinase domain of JAK2 is critical for pathogenic activation

  2015cites this paper
• Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle

  2015cites this paper
• Nucleotide-binding mechanisms in pseudokinases

  2015cites this paper
• Titin kinase is an inactive pseudokinase scaffold that supports MuRF1 recruitment to the sarcomeric M-line

  2014cites this paper
• Synchronous opening and closing motions are essential for cAMP-dependent protein kinase A signaling.

  2014cites this paper
• A Selective NMR Probe to Monitor the Conformational Transition from Inactive to Active Kinase

  2014cites this paper
• ProKinO: A Unified Resource for Mining the Cancer Kinome

  2014cites this paper
• Kinase Regulation by Hydrophobic Spine Assembly in Cancer

  2014cites this paper
• The rise of the undead

  2014cites this paper
• Titin role in muscle homeostasis : the kinase domain

  2014cites this paper
• Herpes Simplex Virus Protein Kinases US3 and UL13 Modulate VP11/12 Phosphorylation, Virion Packaging, and Phosphatidylinositol 3-Kinase/Akt Signaling Activity

  2014cites this paper
• The Arabidopsis ZED1 pseudokinase is required for ZAR1-mediated immunity induced by the Pseudomonas syringae type III effector HopZ1a

  2013influential citation
• A pickup in pseudokinase activity.

  2013cites this paper
• Structure Dynamics Guided Enzyme Improvement of ENDO-BETA-1, 4-XYLANASE I

  2013cites this paper
• Fingerprinting differential active site constraints of ATPases

  2013cites this paper
• It takes two to tango--signalling by dimeric Raf kinases.

  2013cites this paper
• Pseudokinases from a structural perspective.

  2013cites this paper
• Phosphoryl transfer by protein kinase A is captured in a crystal lattice.

  2013cites this paper
• Signaling by Non-Canonical Receptor Tyrosine Kinases ERBB3 and RYK

  2012cites this paper
• The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae.

  2012cites this paper
• Atomistic Insights Into the Regulatory Mechanisms Mediated by Post- Translational Modifications: Molecular Dynamics Investigations

  2012cites this paper
• Purification of protein phosphatases and 14-3-3-binding proteins and their regulation by the cAMP–PKA pathway

  2012cites this paper
• Ascl1‐induced neuronal differentiation of P19 cells requires expression of a specific inhibitor protein of cyclic AMP‐dependent protein kinase

  2012cites this paper
• Assembly of allosteric macromolecular switches: lessons from PKA

  2012cites this paper
• Protein kinase a-dependent phosphorylation of serine 119 in the proto-oncogenic serine/arginine-rich splicing factor 1 modulates its activity as a splicing enhancer protein.

  2011influential citation
• Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain

  2011cites this paper
• The Structural and Functional Identity of the Protein Kinase Superfamily

  2011cites this paper
• Yet another “active” pseudokinase, Erb3

  2010cites this paper
• Enzyme structure dynamics of xylanase I from Trichoderma longibrachiatum

  2010cites this paper
• MUTAGENIC STUDIES OF RDOA, A EUKARYOTIC-LIKE SER/THR PROTEIN KINASE IN SALMONELLA ENTERICA SEROVAR TYPHIMURIUM

  2010influential citation
• Global Consequences of Activation Loop Phosphorylation on Protein Kinase A*

  2009cites this paper
• Ability of PknA, a mycobacterial eukaryotic-type serine/threonine kinase, to transphosphorylate MurD, a ligase involved in the process of peptidoglycan biosynthesis.

  2008cites this paper
• Untersuchung von p38α MAP-Kinase/Inhibitor-Komplexen in Lösung mittels Kernresonanzspektroskopie

  2008cites this paper
• Different requirements of the kinase and UHM domains of KIS for its nuclear localization and binding to splicing factors.

  2008cites this paper
• Substrate specificity of the herpes simplex virus type 2 UL13 protein kinase.

  2008cites this paper
• Structural basis of protein kinase C isoform function.

  2008cites this paper
• Allosteric cooperativity in protein kinase A

  2008cites this paper
• Identification of Functionally Distinct Regions That Mediate Biological Activity of the Protein Kinase A Homolog Tpk2*

  2008cites this paper
• A multi-angular mass spectrometric view at cyclic nucleotide dependent protein kinases: in vivo characterization and structure/function relationships.

  2008cites this paper
• Functional Analysis of the RNA Polymerase II C-terminal Domain Kinase Ctk1 in the Yeast Saccharomyces cerevisiae

  2007cites this paper
• Phosphorylation reaction in cAPK protein kinase-free energy quantum mechanical/molecular mechanics simulations.

  2007cites this paper
• Conservation , Variability and the Modeling of Active Protein Kinases

  2007cites this paper
• Characterization of a eukaryotic type serine/threonine kinase in Spodoptera litura nucleopolyhedrovirus-I (SpltNPV-I).

  2007cites this paper
• Functional Analysis of the RNA Polymerase II C-terminal Domain Kinase Ctk1 in the Yeast Saccharomyces cerevisiae

  2007cites this paper
• Conformational studies of the manganese transport regulator (MntR) from Bacillus subtilis using deuterium exchange mass spectrometry

  2007cites this paper
• Protein‐Targeting Drug Discovery Guided by Hydrogen/Deuterium Exchange Mass Spectrometry (DXMS)

  2007cites this paper
• Conformational Analysis of Epac Activation Using Amide Hydrogen/Deuterium Exchange Mass Spectrometry*

  2007cites this paper
• The kinase homology domain of receptor guanylyl cyclase C: ATP binding and identification of an adenine nucleotide sensitive site.

  2006cites this paper
• Surface comparison of active and inactive protein kinases identifies a conserved activation mechanism

  2006cites this paper
• Structure and dynamics of PKA signaling proteins.

  2006cites this paper
• Structural Determinants for the Binding of Anthrax Lethal Factor to Oligomeric Protective Antigen*

  2006cites this paper
• Allosteric Activation of Coagulation Factor VIIa Visualized by Hydrogen Exchange*

  2006cites this paper
• Unactivated PKR exists in an open conformation capable of binding nucleotides.

  2006cites this paper
• Survey of the year 2005 commercial optical biosensor literature

  2006cites this paper
• Survey of the year 2003 commercial optical biosensor literature

  2005cites this paper
• Dynamics of signaling by PKA.

  2005cites this paper
• Conservation, Variability and the Modeling of Active Protein Kinases

  1995cites this paper