The structure of Bacillus subtilis pectate lyase in complex with calcium

No abstract is available for this paper.

• Publication year

  1994

• Venue

  Nature Structural Biology

• Publication date

  1994-10-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/NSB1094-717PMID 7634076
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Automated refinement of protein models.

  1993cited by this paper
• SQUASH - combining constraints for macromolecular phase refinement and extension.

  1993cited by this paper
• An explanation for rapid enzyme-catalyzed proton abstraction from carbon acids: importance of late transition states in concerted mechanisms

  1993cited by this paper
• Pectate lyase from Bacillus subtilis: molecular characterization of the gene, and properties of the cloned enzyme

  1993cited by this paper
• New domain motif: the structure of pectate lyase C, a secreted plant virulence factor.

  1993cited by this paper
• Unusual structural features in the parallel β-helix in pectate lyases

  1993cited by this paper
• Unusual structural features in the parallel beta-helix in pectate lyases.

  1993cited by this paper
• Crystallization and preliminary X-ray studies of the pectate lyase from Bacillus subtilis.

  1992cited by this paper
• A program to produce both detailed and schematic plots of protein structures

  1991cited by this paper
• Improved methods for building protein models in electron density maps and the location of errors in these models.

  1991cited by this paper
• Purification and characterization of extracellular pectate lyase from Bacillus subtilis.

  1990cited by this paper
• RESTRAIN: restrained structure-factor least-squares refinement program for macromolecular structures

  1989cited by this paper
• Structural aspects of metal ion carboxylate interactions

  1988cited by this paper
• Crystallographic R Factor Refinement by Molecular Dynamics

  1987cited by this paper
• The use of an imaging proportional counter in macromolecular crystallography

  1987cited by this paper
• Using known substructures in protein model building and crystallography.

  1986cited by this paper
• Enzymes and Food Processing

  1980cited by this paper
• The Protein Data Bank: a computer-based archival file for macromolecular structures.

  1977cited by this paper

• The Dynamics, Degradation, and Afterlives of Pectins: Influences on Cell Wall Assembly and Structure, Plant Development and Physiology, Agronomy, and Biotechnology.

  2025cites this paper
• A Low-Temperature-Active Pectate Lyase from a Marine Bacterium for Orange Juice Clarification

  2025cites this paper
• Structure and degradation dynamics of dietary pectin

  2024cites this paper
• A novel pectate lyase with high specific activity from Bacillus sp. B58-2: Gene cloning, heterologous expression and use in ramie degumming.

  2024cites this paper
• Selection of alkaliphilic Bacillus pectate lyases based on reactivity and pH-dependent stability in simulated environment for industrial applications.

  2024cites this paper
• Strength in numbers: An isoform variety of homogalacturonan modifying enzymes may contribute to pollen tube fitness.

  2023cites this paper
• The specificity of pectate lyase VdPelB from Verticilium dahliae is highlighted by structural, dynamical and biochemical characterizations.

  2023cites this paper
• Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay

  2023cites this paper
• Structure of an Alkaline Pectate Lyase and Rational Engineering with Improved Thermo-Alkaline Stability for Efficient Ramie Degumming

  2022cites this paper
• Exogenous calcium application enhances salt tolerance of sweet sorghum seedlings

  2022cites this paper
• Secreted pectin monooxygenases drive plant infection by pathogenic oomycetes

  2021cites this paper
• Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases

  2021cites this paper
• The E34 Phage Tailspike Protein: An in vitro characterization, Structure Prediction, Potential Interaction with S. newington LPS and Cytotoxicity Assessment to Animal Cell Line

  2021cites this paper
• LRRpredictor—A New LRR Motif Detection Method for Irregular Motifs of Plant NLR Proteins Using an Ensemble of Classifiers

  2020cites this paper
• Biochemical characterization and evolutionary analysis of a novel pectate lyase from Aspergillus parasiticus.

  2020cites this paper
• Anti-amyloid drug design

  2020cites this paper
• Enzyme mediated multi-product process: A concept of bio-based refinery

  2020cites this paper
• Solenoid – An amyloid under control

  2020cites this paper
• Origins and features of pectate lyases and their applications in industry

  2020cites this paper
• Pectin Degrading Enzymes

  2020cites this paper
• The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis

  2019cites this paper
• Biochemical and Molecular Characterizations of a Novel pH- and Temperature-Stable Pectate Lyase from Bacillus amyloliquefaciens S6 for Industrial Application

  2019cites this paper
• A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation

  2019cites this paper
• MICROBIAL PECTINASES: A REVIEW ON MOLECULAR AND BIOTECHNOLOGICAL PERSPECTIVES

  2019influential citation
• Symmetry and Dissymmetry in Protein Structure - System-Coding Its Biological Specificity

  2019cites this paper
• Structural insights into the molecular mechanisms of pectinolytic enzymes

  2019cites this paper
• Improving the specific activity and thermo-stability of alkaline pectate lyase from Bacillus subtilis 168 for bioscouring

  2018cites this paper
• Structural basis for the role of serine-rich repeat proteins from Lactobacillus reuteri in gut microbe–host interactions

  2018cites this paper
• Detection and Sequence Analysis of Enzyme Genes of Four Thermo-tolerant Bacillus pumilus Strains from the Philippines

  2018cites this paper
• Molecular Cloning , DNA for Thermostable PectateSequenee , and Expression of the Gene Lyase of Thermophilic BacMus sp . TSEncoding

  2018cites this paper
• SAXS and homology modelling based structure characterization of pectin methylesterase a family 8 carbohydrate esterase from Clostridium thermocellum ATCC 27405.

  2018cites this paper
• Structure-based engineering of a pectate lyase with improved specific activity for ramie degumming

  2017influential citation
• Cloning, evaluation, and high-level expression of a thermo-alkaline pectate lyase from alkaliphilic Bacillus clausii with potential in ramie degumming

  2017cites this paper
• Characterization and overproduction of a thermo-alkaline pectate lyase from alkaliphilic Bacillus licheniformis with potential in ramie degumming.

  2017cites this paper
• A mammalian homolog of the zebrafish transmembrane protein 2 (TMEM2) is the long-sought-after cell-surface hyaluronidase

  2017cites this paper
• Novel Molecular Insights into the Catalytic Mechanism of Marine Bacterial Alginate Lyase AlyGC from Polysaccharide Lyase Family 6*

  2017cites this paper
• Diversity of Three-Dimensional Structures and Catalytic Mechanisms of Alginate Lyases

  2017cites this paper
• The L-type Ca2+ Channel Blocker Nifedipine Inhibits Mycelial Growth, Sporulation, and Virulence of Phytophthora capsici

  2016cites this paper
• QM/MM studies on the calcium‐assisted β‐elimination mechanism of pectate lyase from bacillus subtilis

  2016cites this paper
• Lanthanides: New life metals?

  2016cites this paper
• A Review of natural and engineered enzymes involved in bioethanol production

  2016cites this paper
• The catalytic mechanism and unique low pH optimum of Caldicellulosiruptor bescii family 3 pectate lyase.

  2015cites this paper
• Cloning and Genomic Organization of a Rhamnogalacturonase Gene from Locally Isolated Strain of Aspergillus niger

  2015cites this paper
• Role of Pectinolytic Enzymes Identified in Clostridium thermocellum Cellulosome

  2015cites this paper
• Structural Modelling, Substrate Binding and Stability Studies of Endopectate Lyase (PL1B) of Family 1 Polysaccharide Lyase from Clostridium thermocellum.

  2015cites this paper
• Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3).

  2015influential citation
• Structural insights into the loss of catalytic competence in pectate lyase activity at low pH

  2015cites this paper
• Purification and characterization of a new thermoalkaliphilic pectate lyase from Actinomadura keratinilytica Cpt20

  2015cites this paper
• Cold Active Pectinases: Advancing the Food Industry to the Next Generation

  2014cites this paper
• Homogalacturonan-modifying enzymes: structure, expression, and roles in plants.

  2014influential citation
• Cell wall composition regulates cell shape and growth behaviour in pollen tubes

  2013cites this paper
• The structure and mode of action of Caldicellulosiruptor bescii family 3 pectate lyase in biomass deconstruction.

  2013cites this paper
• Microbial pectinase: sources, characterization and applications

  2013cites this paper
• Potential application of pectinase in developing functional foods.

  2013cites this paper
• Substrate dynamics in enzyme action: rotations of monosaccharide subunits in the binding groove are essential for pectin methylesterase processivity.

  2013cites this paper
• The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastoris is more stable and efficient for degumming ramie fiber

  2013cites this paper
• Improved production of alkaline polygalacturonate lyase by homologous overexpression pelA in Bacillus subtilis

  2013cites this paper
• Expression and Bioinformatics Analysis of Pectate Lyase Gene from Bacillus subtilis521

  2012cites this paper
• Crystal structure and substrate-binding mode of a novel pectate lyase from alkaliphilic Bacillus sp. N16-5.

  2012cites this paper
• Identification of Residues That Underpin Interactions within the Eukaryotic Initiation Factor (eIF2) 2B Complex

  2012cites this paper
• Arg235 is an essential catalytic residue of Bacillus pumilus DKS1 pectate lyase to degum ramie fibre

  2011cites this paper
• Structural studies of three enzymes: telomerase, the methyltransferase CobJ and pectate lyase

  2011cites this paper
• Developing novel enzymes for the laundry industry

  2011cites this paper
• Cloning, expression, and characterization of a highly active alkaline pectate lyase from alkaliphilic Bacillus sp. N16-5.

  2010cites this paper
• The Biochemistry and Structural Biology of Plant Cell Wall Deconstruction

  2010cites this paper
• Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase.

  2010cites this paper
• Biochemical Properties of Pectate Lyases Produced by Three Different Bacillus Strains Isolated from Fermenting Cocoa Beans and Characterization of Their Cloned Genes

  2010cites this paper
• In Silico Characterization of Pectate Lyase Protein Sequences from Different Source Organisms

  2010influential citation
• Cloning , Expression , and Characterization of a Highly Active Alkaline Pectate Lyase from Alkaliphilic Bacillus sp . N 16-5

  2010cites this paper
• Microbial pectate lyases: characterization and enzymological properties

  2009cites this paper
• In silico analysis of pectin lyase and pectinase sequences

  2009cites this paper
• Purification, Characterization, and Overexpression of Thermophilic Pectate Lyase of Bacillus sp. RN1 Isolated from a Hot Spring in Thailand

  2009cites this paper
• Enzymes for the valorisation of fruit- and vegetable-based co-products.

  2009cites this paper
• Prediction of parallel in-register amyloidogenic beta-structures In highly beta-rich protein sequences by pairwise propensity analysis

  2009cites this paper
• The Crystal Structure of Pectate Lyase PelI from Soft Rot Pathogen Erwinia chrysanthemi in Complex with Its Substrate*

  2008cites this paper
• The leucine-rich repeat structure

  2008cites this paper
• Cloning of the Trichoderma reesei cDNA Encoding a Glucuronan Lyase Belonging to a Novel Polysaccharide Lyase Family

  2008cites this paper
• Structural and Biochemical Properties of Pectinases

  2007cites this paper
• Protein aggregation : A perspective from amyloid and inclusion-body formation

  2007cites this paper
• Structural and functional characterization of a novel, host penetration-related pectate lyase from the potato cyst nematode Globodera rostochiensis.

  2007cites this paper
• A review of protein engineering for the food industry.

  2007cites this paper
• A Family 2 Pectate Lyase Displays a Rare Fold and Transition Metal-assisted β-Elimination*

  2007cites this paper
• A Novel Structural Fold in Polysaccharide Lyases

  2007cites this paper
• Heterologous expression and purification of protopectinase-N from Bacillus subtilis in Pichia pastoris

  2006cites this paper
• Hyaluronidases: their genomics, structures, and mechanisms of action.

  2006cites this paper
• Carbohydrate-active Enzymes from Extremophiles

  2006cites this paper
• The role of cell wall-modifying proteins in plant penetration and feeding site proliferation by the potato cyst nematode Globodera rostochiensis

  2006cites this paper
• Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism.

  2005cites this paper
• Epimerase Active Domain of Pseudomonas aeruginosa AlgG, a Protein That Contains a Right-Handed β-Helix

  2005cites this paper
• Crystal Structure of Jun a 1, the Major Cedar Pollen Allergen from Juniperus ashei, Reveals a Parallel β-Helical Core*

  2005cites this paper
• An in vitro evaluation of a thermostable pectate lyase by using error-prone PCR

  2004cites this paper
• Azospirillum irakense pectate lyase displays a toroidal fold.

  2004cites this paper
• Because residues a xed distance in sequence exhibit these correlations in coiled coils

  2004cites this paper
• Kinetic behaviour and thermal inactivation of pectinlyase used in food processing

  2004cites this paper
• Molecular dynamics simulation of the endopolygalacturonase II--octagalacturonate complex

  2004cites this paper
• Low molecular mass pectate lyase from Fusarium moniliforme: similar modes of chemical and thermal denaturation.

  2004cites this paper
• Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative.

  2004cites this paper
• MOLECULAR DYNAMICS SIMULATION OF THE ENDOPOLYGALACTURONASE II— OCTAGALACTURONATE COMPLEX by JARROD WESLEY BARNES

  2004cites this paper
• Molecular studies on protein- and carbohydrate-converting ezymes from thermophilic bacteria

  2004cites this paper
• The endopolygalacturonases from Botrytis cinerea and their interaction with an inhibitor from grapevine

  2004cites this paper