Isolation of the heme-thiolate enzyme cytochrome P-450TYR, which catalyzes the committed step in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench.

No abstract is available for this paper.

• Publication year

  1994

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  1994-10-11

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1073/PNAS.91.21.9740PMID 7937883PMCID PMC44892
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular cloning of an allene oxide synthase: a cytochrome P450 specialized for the metabolism of fatty acid hydroperoxides.

  1993cited by this paper
• Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate 4-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway.

  1993cited by this paper
• Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy

  1993cited by this paper
• N-hydroxylation of benzamidine to benzamidoxime by a reconstituted cytochrome P-450 oxidase system from rabbit liver: involvement of cytochrome P-450 IIC3.

  1993cited by this paper
• Interactions of Avocado (Persea americana) Cytochrome P-450 with Monoterpenoids.

  1992cited by this paper
• Human liver microsomal N-hydroxylation of dapsone by cytochrome P-4503A4.

  1992cited by this paper
• Renal and hepatic microsomal enzymes responsible for bioactivation of 3-methoxy-4-aminoazobenzene in the rodent.

  1991cited by this paper
• Multiple forms of plant cytochromes p-450.

  1991cited by this paper
• 2-nitro-3-(p-hydroxyphenyl)propionate and aci-1-nitro-2-(p-hydroxyphenyl)ethane, two intermediates in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench.

  1991cited by this paper
• Purification of an allene oxide synthase and identification of the enzyme as a cytochrome P-450.

  1991cited by this paper
• Involvement of Cytochrome P-450 in the Biosynthesis of Dhurrin in Sorghum bicolor (L.) Moench.

  1991cited by this paper
• The biosynthesis of cyanogenic glucosides in higher plants. The (E)- and (Z)-isomers of p-hydroxyphenylacetaldehyde oxime as intermediates in the biosynthesis of dhurrin in Sorghum bicolor (L.) Moench.

  1989cited by this paper
• Biosynthesis of the Cyanogenic Glucoside Dhurrin in Seedlings of Sorghum bicolor (L.) Moench and Partial Purification of the Enzyme System Involved.

  1989cited by this paper
• Cytochrome P-450 from the Mesocarp of Avocado (Persea americana).

  1989cited by this paper
• Phytoalexin synthesis in soybean: purification and reconstitution of cytochrome P450 3,9-dihydroxypterocarpan 6a-hydroxylase and separation from cytochrome P450 cinnamate 4-hydroxylase.

  1989cited by this paper
• P450 genes: structure, evolution, and regulation.

  1987cited by this paper
• Acid-labile sulfide and zero-valence sulfur in plant extracts containing chlorophyll and ionic detergents.

  1987cited by this paper
• Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea.

  1986cited by this paper
• The biosynthesis of cyanogenic glucosides in higher plants. N-Hydroxytyrosine as an intermediate in the biosynthesis of dhurrin by Sorghum bicolor (Linn) Moench.

  1979cited by this paper
• Measurement of substrate and inhibitor binding to microsomal cytochrome P-450 by optical-difference spectroscopy.

  1978cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor.

  1975cited by this paper
• The 4-hydroxylation of cinnamic acid by sorghum microsomes and the requirement for cytochrome P-450.

  1974cited by this paper
• THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.

  1964cited by this paper
• Studies on Cyanogenic Glycoside of Sorghum Vulgare.

  1960cited by this paper

• Developing future resilience from signatures of adaptation across the sorghum pangenome

  2025cites this paper
• Unveiling the Potential Role of Dhurrin in Sorghum During Infection by the Head Smut Pathogen Sporisorium reilianum f. sp. reilianum

  2025cites this paper
• Biosynthesis of nitric oxide in plants: An oxidative pathway orchestrated by the interplay of CYP79s, N-OX FMOs, and peroxidases.

  2025cites this paper
• Amino acid substrate specificities and tissue expression profiles of the nine CYP79A encoding genes in Sorghum bicolor

  2025cites this paper
• Sequence diversity in the monooxygenases involved in oxime production in plant defense and signaling: a conservative revision in the nomenclature of the highly complex CYP79 family.

  2024cites this paper
• Nitrile biosynthesis in nature: how and why?

  2024cites this paper
• Pharmaceutical removal from wastewater by introducing cytochrome P450s into microalgae

  2024cites this paper
• Integrated pathway mining and selection of an artificial CYP79-mediated bypass to improve benzylisoquinoline alkaloid biosynthesis

  2024cites this paper
• Transcript profiles of wild and domesticated sorghum under water-stressed conditions and the differential impact on dhurrin metabolism

  2022cites this paper
• Cytochrome P450s in algae: Bioactive natural product biosynthesis and light-driven bioproduction

  2022cites this paper
• Comparative transcriptomics and gene network analysis revealed secondary metabolism as preeminent metabolic pathways for heat tolerance in hard fescue

  2021cites this paper
• Metabolic Engineering of Photosynthetic Cells – in Collaboration with Nature

  2021cites this paper
• Quantification of cyanogenic compounds, amygdalin, prunasin, and hydrocyanic acid in almonds (Prunus dulcis Miller) for industrial uses

  2021cites this paper
• Metabolons and bio-condensates: The essence of plant plasticity and the key elements in development of green production systems

  2021cites this paper
• Discovery of enzymes responsible for cyclization and postmodification in triterpenoid biosynthesis

  2021cites this paper
• Prediction of Dhurrin Metabolism by Transcriptome and Metabolome Analyses in Sorghum

  2020cites this paper
• Molecular snapshots of dynamic membrane-bound metabolons.

  2019cites this paper
• Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent.

  2019cites this paper
• Cyanogenesis in Arthropods: From Chemical Warfare to Nuptial Gifts

  2018influential citation
• Prunasin production using engineered Escherichia coli expressing UGT85A47 from Japanese apricot and UDP-glucose biosynthetic enzyme genes

  2018cites this paper
• Heteroatom-Heteroatom Bond Formation in Natural Product Biosynthesis.

  2017cites this paper
• Assembly of Dynamic P450-Mediated Metabolons—Order Versus Chaos

  2017cites this paper
• Fusion of Ferredoxin and Cytochrome P450 Enables Direct Light-Driven Biosynthesis

  2016cites this paper
• 3. Physicochemical properties, synthesis, applications, and transport

  2016cites this paper
• Biosynthesis, regulation, and significance of cyanogenic glucosides

  2016cites this paper
• Regulation of cyanogenesis in forage sorghum

  2016cites this paper
• Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

  2016cites this paper
• Plasticity of specialized metabolism as mediated by dynamic metabolons.

  2015cites this paper
• Free and total cyanide in environmental samples from Kenyase, a Mining Community in the Brong Ahafo Region of Ghana

  2015cites this paper
• QUANTIFICATION OF TOTAL CYANIDE CONTENT IN KERNELS OF STONE FRUITS

  2015cites this paper
• The bifurcation of the cyanogenic glucoside and glucosinolate biosynthetic pathways.

  2015cites this paper
• Scent emission profiles from Darwin's orchid--Angraecum sesquipedale: Investigation of the aldoxime metabolism using clustering analysis.

  2015cites this paper
• Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in l-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc.

  2014cites this paper
• The Biosynthetic Pathway of Indole-3-Carbaldehyde and Indole-3-Carboxylic Acid Derivatives in Arabidopsis1[W]

  2014cites this paper
• Recent biotechnological progress in enzymatic synthesis of glycosides

  2013cites this paper
• Phytoalexins and other antifungal metabolites from crucifers: isolation, synthesis and biosynthesis

  2013cites this paper
• Amphipol trapping of a functional CYP system

  2013cites this paper
• A combined biochemical screen and TILLING approach identifies mutations in Sorghum bicolor L. Moench resulting in acyanogenic forage production.

  2012cites this paper
• Possible evolution of alliarinoside biosynthesis from the glucosinolate pathway in Alliaria petiolata

  2012cites this paper
• Biosynthesis of rhodiocyanosides in Lotus japonicus: rhodiocyanoside A is synthesized from (Z)-2-methylbutanaloxime via 2-methyl-2-butenenitrile.

  2012cites this paper
• MOLECULAR AND FUNCTIONAL CHARACTERIZATION OF GENES INVOLVED IN SAPONIN BIOSYNTHESIS IN MEDICAGO SPP.

  2012cites this paper
• Cyanide and Carbon Monoxide Ligand Formation in Hydrogenase Biosynthesis

  2011cites this paper
• Conformational changes of the NADPH-dependent cytochrome P450 reductase in the course of electron transfer to cytochromes P450.

  2011cites this paper
• Convergent evolution in biosynthesis of cyanogenic defence compounds in plants and insects

  2011cites this paper
• Characterization and expression profile of two UDP-glucosyltransferases, UGT85K4 and UGT85K5, catalyzing the last step in cyanogenic glucoside biosynthesis in cassava.

  2011cites this paper
• Cyanogenic glucosides in the biological warfare between plants and insects: the Burnet moth-Birdsfoot trefoil model system.

  2011cites this paper
• Hydroxynitrile Lyases: Insights into Biochemistry, Discovery, and Engineering

  2011cites this paper
• Metabolic and molecular aspects of cyanogenesis in apricot seed

  2011cites this paper
• Biosynthesis of Cyanogenic Glycosides, Glucosinolates and Non‐Protein Amino Acids

  2010cites this paper
• Functional diversifications of cyanogenic glucosides.

  2010cites this paper
• Advances in cyanogenic glycosides biosynthesis and analyses in plants: a review.

  2010cites this paper
• 454 pyrosequencing based transcriptome analysis of Zygaena filipendulae with focus on genes involved in biosynthesis of cyanogenic glucosides

  2009cites this paper
• Molecules of Interest Hydroxynitrile glucosides

  2008cites this paper
• beta-Glucosidases as detonators of plant chemical defense.

  2008cites this paper
• Bitterness in Almonds 1(C)(OA)

  2008cites this paper
• Diversification of an ancient theme: hydroxynitrile glucosides.

  2008cites this paper
• Bitterness in Almonds1[C][OA]

  2008cites this paper
• Hydroxynitrile glucosides.

  2008cites this paper
• Cyanogenesis in plants and arthropods.

  2008cites this paper
• Molecules of Interest Cyanogenesis in plants and arthropods

  2008cites this paper
• Cyanogenic glycosides: a case study for evolution and application of cytochromes P450

  2007cites this paper
• The cyanogenic glucoside composition of Zygaena filipendulae (Lepidoptera: Zygaenidae) as effected by feeding on wild-type and transgenic lotus populations with variable cyanogenic glucoside profiles.

  2007cites this paper
• Cyanogenic glycosides: a case study for evolution and application of cytochromes P450

  2006cites this paper
• Purification of recombinant wheat cytochrome P450 monooxygenase expressed in yeast and its properties.

  2006cites this paper
• Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome.

  2005cites this paper
• Metabolon formation and metabolic channeling in the biosynthesis of plant natural products.

  2005cites this paper
• The first cytochrome P450 in ferns

  2005cites this paper
• Cytochrome P450s in Plants

  2005cites this paper
• Biosynthesis of the Nitrile Glucosides Rhodiocyanoside A and D and the Cyanogenic Glucosides Lotaustralin and Linamarin in Lotus japonicus1

  2004cites this paper
• Cyanogenic glucosides and plant-insect interactions.

  2004cites this paper
• Cytotoxic constituents of Alectra and Striga species

  2004cites this paper
• Generation of cyanogen-free transgenic cassava

  2003cites this paper
• GENERATION OF ACYANOGENIC CASSAVA (MANIHOT ESCULENTA CRANTS): TRANSGENIC APPROACHES

  2002cites this paper
• Stoffwechsel cyanogener Glycoside in Linum usitatissimum: Untersuchungen zu Synthese und Transport

  2002cites this paper
• Use of methylotropic yeast Pichia pastoris for expression of cytochromes P450.

  2002cites this paper
• Dhurrin Synthesis in Sorghum Is Regulated at the Transcriptional Level and Induced by Nitrogen Fertilization in Older Plants1

  2002cites this paper
• Resistance to an Herbivore Through Engineered Cyanogenic Glucoside Synthesis

  2001cites this paper
• Metabolism of tyrosine and tryptophan--new genes for old pathways.

  2001cites this paper
• Evolution of metabolic pathways

  2000cites this paper
• Plant cyanogenic glycosides.

  2000cites this paper
• Cloning and expression of cytochrome P450 enzymes catalyzing the conversion of tyrosine to p-hydroxyphenylacetaldoxime in the biosynthesis of cyanogenic glucosides in Triglochin maritima.

  2000cites this paper
• Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes.

  2000cites this paper
• Chapter Seven The biosynthesis, degradation, transport and possible function of cyanogenic glucosides

  2000cites this paper
• Biosynthesis of cyanogenic glucosides in Triglochin maritima and the involvement of cytochrome P450 enzymes.

  1999cites this paper
• The UDP-glucose:p-Hydroxymandelonitrile-O-Glucosyltransferase That Catalyzes the Last Step in Synthesis of the Cyanogenic Glucoside Dhurrin in Sorghum bicolor

  1999cites this paper
• 1.31 – Biosynthesis and Degradation of Cyanogenic Glycosides

  1999cites this paper
• Cyanide in Foods

  1999cites this paper
• Double triton X-114 phase partitioning for the purification of plant cytochromes P450 and removal of green pigments.

  1998cites this paper
• The presence of CYP79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates

  1998cites this paper
• Synthesis and Characterization of Low-Spin Bis(amino ester) Iron(III) Porphyrin Complexes.

  1998cites this paper
• MOLECULAR-GENETIC ANALYSIS OF PLANT CYTOCHROME P450-DEPENDENT MONOOXYGENASES.

  1998cites this paper
• Isolation and Reconstitution of Cytochrome P450ox and in Vitro Reconstitution of the Entire Biosynthetic Pathway of the Cyanogenic Glucoside Dhurrin from Sorghum

  1997cites this paper
• Isolation and Reconstitution of the Heme-Thiolate Protein Obtusifoliol 14α-Demethylase from Sorghum bicolor (L.) Moench*

  1996cites this paper
• Plant Cytochrome P450 Monooxygenases

  1996cites this paper
• Isolation of plant and recombinant CYP79.

  1996cites this paper
• P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature.

  1996cites this paper
• Molecular investigations of plant cytochrome P450.

  1996cites this paper
• Purification and characterization of recombinant cytochrome P450TYR expressed at high levels in Escherichia coli.

  1995cites this paper
• The primary sequence of cytochrome P450tyr, the multifunctional N-hydroxylase catalyzing the conversion of L-tyrosine to p-hydroxyphenylacetaldehyde oxime in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench.

  1995cites this paper
• Characterization of Cytochrome P450TYR, A Multifunctional Haem-Thiolate AZ-Hydroxylase Involved in the Biosynthesis of the Cyanogenic Glucoside Dhurrin

  1995cites this paper