Spatial and temporal patterns of cell division during early Xenopus embryogenesis.

We describe the spatial and temporal patterns of cell division in the early Xenopus embryo, concentrating on the period between the midblastula transition and the early tailbud stage. Mitotic cells were identified using an antibody recognising phosphorylated histone H3. At least four observations are of interest. First, axial mesodermal cells, including prospective notochord, stop dividing after involution and may not divide thereafter. Second, cell division is more pronounced in the neural plate than in nonneural ectoderm, and the pattern of cell division becomes further refined as neurogenesis proceeds. Third, cells in the cement gland cease proliferation completely as they begin to accumulate pigment. Finally, the precursors of peripheral sensory organs such as the ear and olfactory placode undergo active cell proliferation when they arise from the sensorial layer of the ectoderm. These observations and others should provide a platform to study the relationship between the regulation of developmental processes and the cell cycle during Xenopus embryogenesis.

• Publication year

  2001

• Venue

  Developmental Biology

• Publication date

  2001-01-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1006/DBIO.2000.0101PMID 11150237
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A long twentieth century of the cell cycle and beyond.

  2000cited by this paper
• A genetic link between morphogenesis and cell division during formation of the ventral furrow in Drosophila.

  2000influential reference
• Development of neurogenic placodes in Xenopus laevis

  2000cited by this paper
• Tribbles coordinates mitosis and morphogenesis in Drosophila by regulating string/CDC25 proteolysis.

  2000cited by this paper
• Tribbles, a cell-cycle brake that coordinates proliferation and morphogenesis during Drosophila gastrulation.

  2000cited by this paper
• Distinct effects of XBF-1 in regulating the cell cycle inhibitor p27(XIC1) and imparting a neural fate.

  2000influential reference
• Programmed cell death during Xenopus development: a spatio-temporal analysis.

  1998cited by this paper
• Cell cycle transition in early embryonic development of Xenopus laevis.

  1998cited by this paper
• Somitogenesis controlled by Noggin.

  1998cited by this paper
• Coordination of growth and cell division in the Drosophila wing.

  1998cited by this paper
• Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation

  1997cited by this paper
• Analysis of competence and of Brachyury autoinduction by use of hormone-inducible Xbra.

  1997cited by this paper
• Inductive processes leading to inner ear formation during Xenopus development.

  1996cited by this paper
• In vivo regulation of the early embryonic cell cycle in Xenopus.

  1996cited by this paper
• Lateral and axial signals involved in avian somite patterning: a role for BMP4.

  1996cited by this paper
• Regulation of the appearance of division asynchrony and microtubule-dependent chromosome cycles in Xenopus laevis embryos.

  1995cited by this paper
• The Xenopus homologue of Otx2 is a maternal homeobox gene that demarcates and specifies anterior body regions.

  1995cited by this paper
• Ectopic cyclin E expression induces premature entry into S phase and disrupts pattern formation in the Drosophila eye imaginal disc.

  1995cited by this paper
• Control of somite patterning by signals from the lateral plate.

  1995cited by this paper
• Anterior neurectoderm is progressively induced during gastrulation: the role of the Xenopus homeobox gene orthodenticle.

  1995cited by this paper
• Cell cycle remodeling requires cell-cell interactions in developing Xenopus embryos.

  1994cited by this paper
• Mitotic domains in the early embryo of the zebrafish

  1992cited by this paper
• Gastrulation in the mouse: the role of the homeobox gene goosecoid.

  1992cited by this paper
• Neuronal determination without cell division in Xenopus embryos.

  1991cited by this paper
• The Early Development of Xenopus Laevis: An Atlas of the Histology

  1991cited by this paper
• Mitotic domains reveal early commitment of cells in Drosophila embryos.

  1989cited by this paper
• Early neurogenesis in Xenopus: the spatio-temporal pattern of proliferation and cell lineages in the embryonic spinal cord.

  1989cited by this paper
• On the coupling between DNA replication and mitosis

  1989cited by this paper
• The function and mechanism of convergent extension during gastrulation of Xenopus laevis.

  1985cited by this paper
• [Spatial-temporal patterns of the cell cycles in embryos of the clawed toad during gastrulation and neurulation].

  1984cited by this paper
• Duration of cleavage cycles and asymmetry in the direction of cleavage waves prior to gastrulation inXenopus laevis

  1983cited by this paper
• The embryonic cell lineage of the nematode Caenorhabditis elegans.

  1983cited by this paper
• [Mitotic activity of the embryonic tissues of the clawed toad in the period of gastrulation and neurulation].

  1983cited by this paper
• Phosphorylation of histones 1 and 3 and nonhistone high mobility group 14 by an endogenous kinase in HeLa metaphase chromosomes.

  1982cited by this paper
• A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.

  1982influential reference
• A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.

  1982cited by this paper
• Gastrulation in the mouse: assessment of cell populations in the epiblast of tw18/tw18 embryos.

  1978cited by this paper
• ‘METACHRONOUS’ CLEAVAGE AND INITIATION OF GASTRULATION IN AMPHIBIAN EMBRYOS

  1977cited by this paper
• Properties of the primary organization field in the embryo of Xenopus laevis. IV. Pattern formation and regulation following early inhibition of mitosis.

  1973influential reference
• Changes in the cell cycle during early amphibian development

  1966cited by this paper
• Normal Table of Xenopus Laevis (Daudin)

  1958cited by this paper

• Vestigial-like 4 Regulates Neurogenesis and Neural Crest Formation During Xenopus Development.

  2026cites this paper
• The Effects of Photobiomodulation Therapy on Xenopus laevis Embryonic Epithelium

  2025cites this paper
• Differential sensitivity of midline development to mitosis during and after primitive streak extension.

  2025cites this paper
• The Xenopus Oocyte System: Molecular Dynamics of Maturation, Fertilization, and Post-Ovulatory Fate

  2025cites this paper
• Differential Sensitivity of Midline Patterning to Mitosis during and after Primitive Streak Extension

  2024cites this paper
• The Zebrafish Retina and the Evolution of the Onecut-Mediated Pathway in Cell Type Differentiation

  2024cites this paper
• Diphthamide deficiency promotes association of eEF2 with p53 to induce p21 expression and neural crest defects

  2024cites this paper
• V-ATPase Regulates Retinal Progenitor Cell Proliferation During Eye Regrowth in Xenopus

  2023cites this paper
• Xenopus: An in vivo model for studying skin response to ultraviolet B irradiation

  2023cites this paper
• Wnt11 family dependent morphogenesis during frog gastrulation is marked by the cleavage furrow protein anillin

  2022cites this paper
• Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor

  2022cites this paper
• Retinoic acid production, regulation and containment through Zic1, Pitx2c and Cyp26c1 control cranial placode specification

  2021cites this paper
• Function of chromatin modifier Hmgn1 during neural crest and craniofacial development

  2021cites this paper
• Transitions in the proteome and phospho-proteome during Xenopus laevis development

  2021cites this paper
• The Use of Xenopus for Cell Biology Applications.

  2021cites this paper
• Morphogenetic mechanisms forming the notochord rod: The turgor pressure‐sheath strength model

  2020cites this paper
• Mcl1 protein levels and Caspase‐7 executioner protease control axial organizer cells survival

  2020cites this paper
• Surface contraction waves or cell proliferation waves in the presumptive neurectoderm during amphibian gastrulation: Mexican axolotl versus African clawed frog

  2020cites this paper
• Interplay of TRIM2 E3 Ubiquitin Ligase and ALIX/ESCRT Complex: Control of Developmental Plasticity During Early Neurogenesis

  2020cites this paper
• Lignans from Bursera fagaroides Affect In Vivo Cell Behavior by Disturbing the Tubulin Cytoskeleton in Zebrafish Embryos

  2018cites this paper
• STRUCTURAL AND MOLECULAR REGULATORS OF EMBRYONIC TISSUE STIFFNESS

  2018influential citation
• MMP14 Regulates Cranial Neural Crest Epithelial‐to‐Mesenchymal Transition and Migration

  2018cites this paper
• A model for investigating developmental eye repair in Xenopus laevis

  2018cites this paper
• Multiscale analysis of architecture, cell size and the cell cortex reveals cortical F-actin density and composition are major contributors to mechanical properties during convergent extension

  2018cites this paper
• A dual function of FGF signaling in Xenopus left-right axis formation

  2018cites this paper
• Analysis of Cell Size in the Gastrula of Ten Frog Species Reveals a Correlation of Egg with Cell Sizes, and a Conserved Pattern of Small Cells in the Marginal Zone

  2017cites this paper
• Ingression-type cell migration drives vegetal endoderm internalisation in the Xenopus gastrula

  2017cites this paper
• Human amniotic fluid contaminants alter thyroid hormone signalling and early brain development in Xenopus embryos

  2017cites this paper
• Leptin Induces Mitosis and Activates the Canonical Wnt/β-Catenin Signaling Pathway in Neurogenic Regions of Xenopus Tadpole Brain

  2017cites this paper
• Research proceedings on amphibian model organisms.

  2016cites this paper
• Sf3b4-depleted Xenopus embryos: A model to study the pathogenesis of craniofacial defects in Nager syndrome.

  2016cites this paper
• Neil DNA glycosylases promote substrate turnover by Tdg during DNA demethylation

  2016cites this paper
• Functional analysis of Hairy genes in Xenopus neural crest initial specification and cell migration

  2015cites this paper
• Kcnip1 a Ca²⁺-dependent transcriptional repressor regulates the size of the neural plate in Xenopus.

  2015cites this paper
• Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos

  2015cites this paper
• Endogenous Gradients of Resting Potential Instructively Pattern Embryonic Neural Tissue via Notch Signaling and Regulation of Proliferation

  2015cites this paper
• Pou5f3.2‐induced proliferative state of embryonic cells during gastrulation of Xenopus laevis embryo

  2015influential citation
• An oncologist׳s friend: How Xenopus contributes to cancer research

  2015cites this paper
• Suppression of vascular network formation by chronic hypoxia and prolyl-hydroxylase 2 (phd2) deficiency during vertebrate development

  2015cites this paper
• Brain region-specific microglial phenotypes and responses in Parkinson’s disease

  2015cites this paper
• Local and long-range endogenous resting potential gradients antagonistically regulate apoptosis and proliferation in the embryonic CNS.

  2015cites this paper
• Essential Roles of Stat3 in Zebrafish Development

  2015cites this paper
• GEF-H1 functions in apical constriction and cell intercalations and is essential for vertebrate neural tube closure

  2014cites this paper
• The Xenopus Embryo as a Model System to Study Asymmetric Furrowing in Vertebrate Epithelial Cells

  2014cites this paper
• Temperature-independent energy expenditure in early development of the African clawed frog Xenopus laevis

  2014cites this paper
• Hippocampal Proliferation Is Increased in Presymptomatic Parkinson's Disease and due to Microglia

  2014cites this paper
• The cell cycle and pluripotency

  2013cites this paper
• The Syncytial Drosophila Embryo as a Mechanically Excitable Medium

  2013cites this paper
• Comparative Functional Analysis of ZFP36 Genes during Xenopus Development

  2013cites this paper
• Lamprey neural Helix-Loop-Helix (HLH) genes and the evolution of the vertebrate nervous system

  2013cites this paper
• Cell Proliferation Patterns in Early Zebrafish Development

  2013cites this paper
• Maturin is a novel protein required for differentiation during primary neurogenesis.

  2013cites this paper
• The RNA-binding protein XSeb4R regulates maternal Sox3 at the posttranscriptional level during maternal-zygotic transition in Xenopus.

  2012cites this paper
• Antibody development and use in chromogenic and fluorescent immunostaining.

  2012cites this paper
• Xaml1/Runx1 is required for the specification of Rohon-Beard sensory neurons in Xenopus.

  2012cites this paper
• Homeoprotein hhex-induced conversion of intestinal to ventral pancreatic precursors results in the formation of giant pancreata in Xenopus embryos

  2012influential citation
• Exploring the role of caspases and RIPK4 during Xenopus development

  2011cites this paper
• Temporal and spatial expression patterns of Cdc25 phosphatase isoforms during early Xenopus development.

  2011cites this paper
• The role of histone variants and hnRNPs in early Xenopus laevis development

  2011cites this paper
• Negative autoregulation of Oct3/4 through Cdx1 promotes the onset of gastrulation

  2011cites this paper
• Gadd45a and Gadd45g regulate neural development and exit from pluripotency in Xenopus.

  2011cites this paper
• Control of DNA replication by cyclin-dependent kinases in development.

  2011cites this paper
• Regulation of morphogen signalling during neural patterning in the Xenopus embryo

  2011cites this paper
• Pronephric tubulogenesis requires Daam1-mediated planar cell polarity signaling.

  2011cites this paper
• A medaka model to study the the molecular basis of Microphthalmia with Linear Skin defects (MLS) syndrome

  2010cites this paper
• Studies of the Tissues and Molecules That Regulate the Migration of Cranial Neural Crest Cells in the Chicken Embryo: Roles of Midline 1 and Retinoic Acid

  2010cites this paper
• Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3

  2010cites this paper
• Temporal and spatial patterning of axial myotome fibers in Xenopus laevis

  2010cites this paper
• Decreased numbers of progenitor cells but no response to antidepressant drugs in the hippocampus of elderly depressed patients.

  2010cites this paper
• Induction of Vertebrate Regeneration by a Transient Sodium Current

  2010cites this paper
• The F-box protein Cdc4/Fbxw7 is a novel regulator of neural crest development in Xenopus laevis

  2010cites this paper
• Investigation of mechanisms regulating cell proliferation in the zebrafish developmental program

  2010cites this paper
• Identification and characterization of genes involved in mouse embryonic stem cell differentiation into neurons

  2010cites this paper
• Transmembrane potential of GlyCl-expressing instructor cells induces a neoplastic-like conversion of melanocytes via a serotonergic pathway

  2010cites this paper
• EUROPEAN SCHOOL OF MOLECULAR MEDICINE

  2010cites this paper
• BrunoL 1 regulates endoderm proliferation through translational enhancement of cyclin A 2 mRNA

  2010cites this paper
• Making senses development of vertebrate cranial placodes.

  2010cites this paper
• Long‐term consequences of Sox9 depletion on inner ear development

  2010cites this paper
• BrunoL1 regulates endoderm proliferation through translational enhancement of cyclin A2 mRNA.

  2010cites this paper
• Survey of O-GlcNAc level variations in Xenopus laevis from oogenesis to early development

  2009cites this paper
• Islet-1 is required for ventral neuron survival in Xenopus.

  2009cites this paper
• The role of Xenopus Rx-L in photoreceptor cell determination.

  2009cites this paper
• Thyroid hormone receptor subtype specificity for hormone-dependent neurogenesis in Xenopus laevis.

  2009cites this paper
• miR-290 acts as a physiological effector of senescence in mouse embryo fibroblasts.

  2009cites this paper
• The mechanism and pattern of yolk consumption provide insight into embryonic nutrition in Xenopus

  2009cites this paper
• microRNA-24a is required to repress apoptosis in the developing neural retina.

  2009cites this paper
• In vivo imaging reveals a role for Cdc42 in spindle positioning and planar orientation of cell divisions during vertebrate neural tube closure

  2009cites this paper
• FoxM1-driven cell division is required for neuronal differentiation in early Xenopus embryos

  2008cites this paper
• Psf2 plays important roles in normal eye development in Xenopus laevis

  2008cites this paper
• Pnas4 is a novel regulator for convergence and extension during vertebrate gastrulation

  2008cites this paper
• Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis.

  2008cites this paper
• Study of the physiological function of three putative modifiers of the p53 pathway: Nucleostemin, Ptprv and TCTP

  2008cites this paper
• Eya1 and Six1 promote neurogenesis in the cranial placodes in a SoxB1-dependent fashion.

  2008cites this paper
• RASSF7 is a member of a new family of RAS association domain-containing proteins and is required for completing mitosis.

  2008cites this paper
• Mechanisms of brain ventricle development

  2008cites this paper
• Developmental expression of Cyclin H and Cdk7 in zebrafish: the essential role of Cyclin H during early embryo development

  2007cites this paper
• The small GTPase RhoV is an essential regulator of neural crest induction in Xenopus.

  2007cites this paper
• Apoptosis is required during early stages of tail regeneration in Xenopus laevis.

  2007cites this paper
• Pituitary-interrenal interaction in zebrafish interrenal organ development.

  2007influential citation
• Xeya3 regulates survival and proliferation of neural progenitor cells within the anterior neural plate of Xenopus embryos

  2007cites this paper