Bartonella Adhesin A Mediates a Proangiogenic Host Cell Response

Bartonella henselae causes vasculoproliferative disorders in humans. We identified a nonfimbrial adhesin of B. henselae designated as Bartonella adhesin A (BadA). BadA is a 340-kD outer membrane protein encoded by the 9.3-kb badA gene. It has a modular structure and contains domains homologous to the Yersinia enterocolitica nonfimbrial adhesin (Yersinia adhesin A). Expression of BadA was restored in a BadA-deficient transposon mutant by complementation in trans. BadA mediates the binding of B. henselae to extracellular matrix proteins and to endothelial cells, possibly via β1 integrins, but prevents phagocytosis. Expression of BadA is crucial for activation of hypoxia-inducible factor 1 in host cells by B. henselae and secretion of proangiogenic cytokines (e.g., vascular endothelial growth factor). BadA is immunodominant in B. henselae–infected patients and rodents, indicating that it is expressed during Bartonella infections. Our results suggest that BadA, the largest characterized bacterial protein thus far, is a major pathogenicity factor of B. henselae with a potential role in the induction of vasculoproliferative disorders.

• Publication year

  2004

• Venue

  Journal of Experimental Medicine

• Publication date

  2004-11-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/jem.20040500PMID 15534369PMCID 2211922
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The VirB type IV secretion system of Bartonella henselae mediates invasion, proinflammatory activation and antiapoptotic protection of endothelial cells

  2004cited by this paper
• The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae.

  2004cited by this paper
• The Yersinia adhesin YadA collagen‐binding domain structure is a novel left‐handed parallel β‐roll

  2004influential reference
• Insulin‐like growth factor binding protein‐3 is overexpressed in endothelial cells of mouse breast tumor vessels

  2003cited by this paper
• Molecular Analysis of Transport and Oligomerization of the Yersinia enterocolitica Adhesin YadA

  2003cited by this paper
• Rapid and efficient transposon mutagenesis of Bartonella henselae by transposome technology.

  2003influential reference
• Regulation of angiogenesis by hypoxia: role of the HIF system

  2003cited by this paper
• Bartonella-associated endothelial proliferation depends on inhibition of apoptosis

  2002cited by this paper
• Induction of a Potential Paracrine Angiogenic Loop between Human THP-1 Macrophages and Human Microvascular Endothelial Cells during Bartonella henselae Infection

  2002cited by this paper
• Alpha 5 beta 1 integrin activates an NF-kappa B-dependent program of gene expression important for angiogenesis and inflammation.

  2002cited by this paper
• NadA, a Novel Vaccine Candidate of Neisseria meningitidis

  2002influential reference
• Adrenomedullin promotes formation of xenografted endometrial tumors by stimulation of autocrine growth and angiogenesis

  2002cited by this paper
• α5β1 Integrin Activates an NF-κB-Dependent Program of Gene Expression Important for Angiogenesis and Inflammation

  2002cited by this paper
• Fibronectin mediates Opc‐dependent internalization of Neisseria meningitidis in human brain microvascular endothelial cells

  2002cited by this paper
• YadA, the multifaceted Yersinia adhesin.

  2001cited by this paper
• Bartonella henselae Induces NF-κB-Dependent Upregulation of Adhesion Molecules in Cultured Human Endothelial Cells: Possible Role of Outer Membrane Proteins as Pathogenic Factors

  2001cited by this paper
• Evidence of a leading role for VEGF in Bartonella henselae‐induced endothelial cell proliferations

  2001cited by this paper
• Yersinia enterocolitica invasin protein triggers IL‐8 production in epithelial cells via activation of Rel p65‐p65 homodimers

  2000cited by this paper
• Structure and sequence analysis of Yersinia YadA and Moraxella UspAs reveal a novel class of adhesins

  2000influential reference
• The PSIPRED protein structure prediction server

  2000cited by this paper
• Interaction of Bartonella henselae with endothelial cells results in rapid bacterial rRNA synthesis and replication

  2000influential reference
• Vascular-specific growth factors and blood vessel formation

  2000cited by this paper
• Outer membrane proteins ofBartonella henselaeand their interaction with human endothelial cells

  1998cited by this paper
• Outer membrane proteins of Bartonella henselae and their interaction with human endothelial cells.

  1998cited by this paper
• Rapid 'de novo' peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer.

  1997cited by this paper
• Bartonella spp. as emerging human pathogens

  1997cited by this paper
• Beta 1 integrin-dependent and -independent polymerization of fibronectin

  1996cited by this paper
• Bartonella quintana invades and multiplies within endothelial cells in vitro and in vivo and forms intracellular blebs.

  1996influential reference
• New serotype of Bartonella heriselae in endocarditis and cat-scratch disease

  1996cited by this paper
• Deletion of amino acids 29 to 81 in adhesion protein YadA of Yersinia enterocolitica serotype O:8 results in selective abrogation of adherence to neutrophils

  1996cited by this paper
• Lack of beta 1 integrin gene in embryonic stem cells affects morphology, adhesion, and migration but not integration into the inner cell mass of blastocysts

  1995cited by this paper
• Hypoxia-induced Transcriptional Activation and Increased mRNA Stability of Vascular Endothelial Growth Factor in C6 Glioma Cells (*)

  1995cited by this paper
• Pathogenesis of defined invasion mutants of Yersinia enterocolitica in a BALB/c mouse model of infection

  1995cited by this paper
• Bartonella henselae and Bartonella quintana adherence to and entry into cultured human epithelial cells

  1995influential reference
• pBBR1MCS: a broad-host-range cloning vector.

  1994cited by this paper
• Rochalimaea species stimulate human endothelial cell proliferation and migration in vitro.

  1994cited by this paper
• Role of YadA in resistance of Yersinia enterocolitica to phagocytosis by human polymorphonuclear leukocytes

  1994influential reference
• The Yersinia pseudotuberculosis adhesin YadA mediates intimate bacterial attachment to and entry into HEp-2 cells

  1993influential reference
• Sex preferences in Turkey.

  1993cited by this paper
• Role of plasmid-encoded antigens of Yersinia enterocolitica in humoral immunity against secondary Y. enterocolitica infection in mice.

  1993cited by this paper
• Plasmid-encoded outer membrane protein YadA mediates specific binding of enteropathogenic yersiniae to various types of collagen

  1992cited by this paper
• Isolation of Rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis.

  1992cited by this paper
• Interleukin-8 as a macrophage-derived mediator of angiogenesis.

  1992cited by this paper
• Predicting coiled coils from protein sequences

  1991cited by this paper
• A workbench for multiple alignment construction and analysis

  1991cited by this paper
• The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens.

  1990cited by this paper
• A specific radioimmunoassay for the growth hormone (GH)-dependent somatomedin-binding protein: its use for diagnosis of GH deficiency.

  1990cited by this paper
• Molecular architecture of basement membranes

  1990cited by this paper

• Determination of the genome-scale metabolic network of Bartonella quintana str. Toulouse to optimize growth for its use as chassis for synthetic biology

  2025cites this paper
• Hypoxia drives progression of multiple sclerosis by enhancing the inflammasome activation in macrophages with Porphyromonas gingivalis infection

  2025cites this paper
• Bartonella henselae and Bartonella quintana antigens grown in liquid medium are inferior to cell culture-grown antigen for immunofluorescence IgG testing of patient sera

  2025cites this paper
• Bartonella henselae: A challenging diagnosis with a bright future.

  2025cites this paper
• Immunofluorescence analysis reveals no increased seroprevalence of anti-Bartonella schoenbuchensis-IgG antibodies in German forest workers

  2025cites this paper
• Comparison of transcriptomic profiles between intracellular and extracellular Bartonella henselae

  2025cites this paper
• Update on Common Bartonella Infections

  2024cites this paper
• Parasite contingency loci and the evolution of host specificity: Simple sequence repeats mediate Bartonella adaptation to a wild rodent host

  2024influential citation
• Genome sequences of three Bartonella schoenbuchensis strains from Czechia

  2024cites this paper
• The Trimeric Autotransporter Adhesin EmaA and Infective Endocarditis

  2024cites this paper
• Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5

  2024cites this paper
• Differential vasoproliferative traits of Bartonella henselae strains associated with autotransporter BafA variants

  2024influential citation
• Pathogen contingency loci and the evolution of host specificity: Simple sequence repeats mediate Bartonella adaptation to a wild rodent host

  2024cites this paper
• Sneaky tactics: Ingenious immune evasion mechanisms of Bartonella

  2024cites this paper
• Virulence-linked adhesin drives mutualist colonization of the bee gut via biofilm formation

  2024cites this paper
• Metagenomics of culture isolates and insect tissue illuminate the evolution of Wolbachia, Rickettsia and Bartonella symbionts in Ctenocephalides spp. fleas

  2023cites this paper
• Advancements in understanding the molecular and immune mechanisms of Bartonella pathogenicity

  2023cites this paper
• Comparison of Biological Activities of BafA Family Autotransporters within Bartonella Species Derived from Cats and Rodents

  2023cites this paper
• Metagenomics of culture isolates and insect tissue illuminate the evolution of Wolbachia, Rickettsia and Bartonella symbionts in Ctenocephalides spp. fleas

  2023cites this paper
• Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes

  2023cites this paper
• Adhesion of human pathogenic bacteria to endothelial cells is facilitated by fibronectin interaction.

  2023cites this paper
• Crosstalk between hypoxic cellular micro-environment and the immune system: a potential therapeutic target for infectious diseases

  2023cites this paper
• Pan-genome wide association study of Glaesserella parasuis highlights genes associated with virulence and biofilm formation

  2023cites this paper
• Limitations of Serological Diagnosis of Typical Cat Scratch Disease and Recommendations for the Diagnostic Procedure

  2023cites this paper
• The autotransporter BafA contributes to the proangiogenic potential of Bartonella elizabethae

  2023cites this paper
• Bartonella taylorii: A Model Organism for Studying Bartonella Infection in vitro and in vivo

  2022cites this paper
• Interaction of Bartonella henselae with Fibronectin Represents the Molecular Basis for Adhesion to Host Cells

  2022cites this paper
• Long-Read Sequencing Reveals Genetic Adaptation of Bartonella Adhesin A Among Different Bartonella henselae Isolates

  2022cites this paper
• Mechanical Forces Govern Interactions of Host Cells with Intracellular Bacterial Pathogens

  2022cites this paper
• Coxiella burnetii Affects HIF1α Accumulation and HIF1α Target Gene Expression

  2022cites this paper
• The Clinical Profile of Cat-Scratch Disease’s Neuro-Ophthalmological Effects

  2022cites this paper
• Adhesion of Bartonella henselae to Fibronectin Is Mediated via Repetitive Motifs Present in the Stalk of Bartonella Adhesin A

  2022cites this paper
• Bartonellosis in Dogs and Cats, an Update.

  2022cites this paper
• Categorizing Sequences of Concern by Function To Better Assess Mechanisms of Microbial Pathogenesis

  2021cites this paper
• Platelet PD-L1 reflects collective intratumoral PD-L1 expression and predicts immunotherapy response in non-small cell lung cancer

  2021cites this paper
• CEACAM1 Activation by CbpF-Expressing E. coli

  2021cites this paper
• Single-cell adhesion force mapping of a highly sticky bacterium in liquid.

  2021cites this paper
• Microscale communication between bacterial pathogens and the host epithelium

  2021cites this paper
• What Is in a Cat Scratch? Growth of Bartonella henselae in a Biofilm

  2021cites this paper
• Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis

  2020cites this paper
• Coaggregation properties of trimeric autotransporter adhesins

  2020cites this paper
• A sticky bacterium versus antiadhesive surfaces: The adhesion preference of bacteria expressing trimeric autotransporter adhesin

  2020cites this paper
• The Bartonella autotransporter BafA activates the host VEGF pathway to drive angiogenesis

  2020cites this paper
• Mechanisms controlling bacterial infection in myeloid cells under hypoxic conditions

  2020cites this paper
• HIF-1α is involved in blood–brain barrier dysfunction and paracellular migration of bacteria in pneumococcal meningitis

  2020cites this paper
• The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii

  2019influential citation
• A Small RNA and DNA Binding Protein Contribute to Biofilm Development in Bartonella henselae

  2019cites this paper
• Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

  2019cites this paper
• Carrion’s disease: more than a neglected disease

  2019cites this paper
• Immunogenicity of trimeric autotransporter adhesins and their potential as vaccine targets

  2019cites this paper
• Leishmania Infection Induces Macrophage Vascular Endothelial Growth Factor A Production in an ARNT/HIF-Dependent Manner

  2019cites this paper
• Type V Secretion Systems: An Overview of Passenger Domain Functions

  2019cites this paper
• The trimeric autotransporter adhesin BadA is required for in vitro biofilm formation by Bartonella henselae

  2019influential citation
• Prophage-Driven Genomic Structural Changes Promote Bartonella Vertical Evolution

  2018cites this paper
• Molecular Mechanisms of Bartonella and Mammalian Erythrocyte Interactions: A Review

  2018cites this paper
• Bacterial riboswitches and RNA thermometers: Nature and contributions to pathogenesis

  2018cites this paper
• Development of a Specific and Sensitive Enzyme-Linked Immunosorbent Assay as an In Vitro Diagnostic Tool for Detection of Bartonella henselae Antibodies in Human Serum

  2018influential citation
• Bacterial Periplasmic Oxidoreductases Control the Activity of Oxidized Human Antimicrobial β-Defensin 1

  2018cites this paper
• Investigation of Bartonella quintana Host Immune Evasion

  2018cites this paper
• Hypoxia and HIF-1 activation in bacterial infections.

  2017cites this paper
• Molecular detection of Bartonella henselae in 11 Ixodes ricinus ticks extracted from a single cat

  2017cites this paper
• Ubiquitously expressed Human Beta Defensin 1 (hBD1) forms bacteria-entrapping nets in a redox dependent mode of action

  2017cites this paper
• Genomic changes associated with the evolutionary transition of an insect gut symbiont into a blood-borne pathogen

  2017cites this paper
• Bartonella Species, an Emerging Cause of Blood-Culture-Negative Endocarditis

  2017influential citation
• Isolation and screening of proangiogenic and antiangiogenic metabolites producing rare actinobacteria from soil

  2017cites this paper
• An integrative strategy to identify the entire protein coding potential of prokaryotic genomes by proteogenomics

  2017cites this paper
• The repeat structure of two paralogous genes, Yersinia ruckeri invasin (yrInv) and a "Y. ruckeri invasin-like molecule", (yrIlm) sheds light on the evolution of adhesive capacities of a fish pathogen.

  2017cites this paper
• Immunosuppressive and angiogenic cytokine profile associated with Bartonella bacilliformis infection in post-outbreak and endemic areas of Carrion's disease in Peru

  2017cites this paper
• ก Expression and purification of Bartonella henselae VirB protein การผลิตและทําาให้มีความบริสุทธิ์ของเชื้อบาโทเนลลา เฮนเซเลเวอร์บี โปรตีน บทคัดย่อ ารศึกษาครั้งนี้ มีวัตถุประสงค์เพื่อศึกษาความเป็นไปได้ของการนําาเวอร์บียีนจากเชื้อบาโทเนลลา เฮนเซนเล มาใช้ในการวินิจฉัย โรคแมวขว่นทีม่สีาเหตมุาจากเชือ้นี้

  2017cites this paper
• Lymphatic Circulation Disseminates Bartonella Infection Into Bloodstream.

  2016cites this paper
• Characterization of the general stress response in Bartonella henselae.

  2016cites this paper
• A family of genus‐specific RNAs in tandem with DNA‐binding proteins control expression of the badA major virulence factor gene in Bartonella henselae

  2016influential citation
• Development of a serum-free liquid medium for Bartonella species

  2016cites this paper
• Quantitative PCR Analysis of the Bartonella henselae carD Gene during Bacterial Stress

  2016cites this paper
• An Acinetobacter trimeric autotransporter adhesin reaped from cells exhibits its nonspecific stickiness via a highly stable 3D structure

  2016cites this paper
• Structural Investigation of Antigens Using Electron Microscopy

  2016cites this paper
• Whole-genome sequence analysis and exploration of the zoonotic potential of a rat-borne Bartonella elizabethae.

  2016cites this paper
• New findings on the function and potential applications of the trimeric autotransporter adhesin

  2015cites this paper
• Proteins of Bartonella bacilliformis: Candidates for Vaccine Development

  2015cites this paper
• The Giant Adhesin SiiE of Salmonella enterica

  2015cites this paper
• Adhesion of Bacteria

  2015cites this paper
• Reprogramming of myeloid angiogenic cells by Bartonella henselae leads to microenvironmental regulation of pathological angiogenesis

  2015cites this paper
• Characterization of Candidatus Bartonella ancashi: A Novel Human Pathogen Associated with Carrins Disease

  2015cites this paper
• Short report Open Access

  2015cites this paper
• Analysis of Endothelial Adherence of Bartonella henselae and Acinetobacter baumannii Using a Dynamic Human Ex Vivo Infection Model

  2015cites this paper
• A domain dictionary of trimeric autotransporter adhesins.

  2015cites this paper
• Characterization of Two Novel Gene Regulatory Systems in the Zoonotic Bacterium Bartonella henselae

  2015cites this paper
• Heterologous Expression of Bartonella Adhesin A in Escherichia coli by Exchange of Trimeric Autotransporter Adhesin Domains Results in Enhanced Adhesion Properties and a Pathogenic Phenotype

  2014influential citation
• The Family Bartonellaceae

  2014cites this paper
• Les autotransporteurs auto-associatifs d’Escherichia coli : de facteurs de virulence à déterminants sociaux

  2014cites this paper
• Blood Platelets in the Progression of Alzheimer’s Disease

  2014cites this paper
• Proteome-wide identification of predominant subcellular protein localizations in a bacterial model organism.

  2014cites this paper
• Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical

  2014cites this paper
• Oroya Fever and Verruga Peruana: Bartonelloses Unique to South America

  2014influential citation
• Bartonella henselae Infection and Host Response in the Zebrafish Embryo Model

  2014influential citation
• Zebrafish Embryo Model of Bartonella henselae Infection

  2014influential citation
• Role of ARF6, Rab11 and External Hsp90 in the Trafficking and Recycling of Recombinant-Soluble Neisseria meningitidis Adhesin A (rNadA) in Human Epithelial Cells

  2014cites this paper
• Uncovering the transcriptional control of "Bartonella henselae" host adaptation factors

  2014cites this paper
• Proteinaceous determinants of surface colonization in bacteria: bacterial adhesion and biofilm formation from a protein secretion perspective

  2013cites this paper
• Autotransporter secretion: varying on a theme.

  2013cites this paper