SNP Genotyping Defines Complex Gene-Flow Boundaries Among African Malaria Vector Mosquitoes

Signals of Mosquito Speciation Malaria in Africa is transmitted by the mosquito species complex Anopheles gambiae. Neafsey et al. (p. 514) made high-resolution single-nucleotide arrays to map genetic divergence among members of the species. Differentiation between populations was observed and evidence obtained for selective sweeps within populations. Most divergence occurred within inversion regions around the centrosome and in genes associated with development, pheromone signaling, and from the X chromosome. The analysis also revealed signals of sympatric speciation occurring within similar chromosomal regions in mosquitoes from different regions in Africa. Lawniczak et al. (p. 512) sequenced the genomes of two molecular forms (known as M and S) of A. gambiae, which have distinctive behavioral phenotypes and appear to be speciating. This effort resolves problems arising from the apparently chimeric nature of the reference genome and confirms the observed genome-wide divergences. This kind of analysis has the potential to contribute to control programs that can adapt to population shifts in mosquito behavior arising from the selective effects of the control measures themselves. Populations of African malaria vectors show signs of selective sweeps and ongoing speciation in their genomes. Mosquitoes in the Anopheles gambiae complex show rapid ecological and behavioral diversification, traits that promote malaria transmission and complicate vector control efforts. A high-density, genome-wide mosquito SNP-genotyping array allowed mapping of genomic differentiation between populations and species that exhibit varying levels of reproductive isolation. Regions near centromeres or within polymorphic inversions exhibited the greatest genetic divergence, but divergence was also observed elsewhere in the genomes. Signals of natural selection within populations were overrepresented among genomic regions that are differentiated between populations, implying that differentiation is often driven by population-specific selective events. Complex genomic differentiation among speciating vector mosquito populations implies that tools for genome-wide monitoring of population structure will prove useful for the advancement of malaria eradication.

• Publication year

  2010

• Venue

  Science

• Publication date

  2010-09-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1126/science.1193036PMID 20966254PMCID PMC4811326
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Malaria management: past, present, and future.

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• Natural selection in action during speciation

  2009cited by this paper
• Differential gene expression in incipient species of Anopheles gambiae

  2008cited by this paper
• The molecular forms of Anopheles gambiae: a phenotypic perspective.

  2008cited by this paper
• Molecular and cellular components of the mating machinery in Anopheles gambiae females

  2008cited by this paper
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  2007cited by this paper
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  2006cited by this paper
• Evidence for subdivision within the M molecular form of Anopheles gambiae

  2006cited by this paper
• On the distribution and genetic differentiation of Anopheles gambiae s.s. molecular forms.

  2005cited by this paper
• Recombination and speciation

  2005cited by this paper
• Genomic scans for selective sweeps using SNP data.

  2005cited by this paper
• Centromere-proximal differentiation and speciation in Anopheles gambiae.

  2005cited by this paper
• Genomic Islands of Speciation in Anopheles gambiae

  2005cited by this paper
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• IL-13受体α2降低血吸虫病肉芽肿的炎症反应并延长宿主存活时间[英]/Mentink-Kane MM,Cheever AW，Thompson RW，et al//Proc Natl Acad Sci U S A

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  2004cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2001cited by this paper
• Evolution

  1996cited by this paper
• Spatial distribution of chromosomal inversions and speciation in Anopheline mosquitoes.

  1982cited by this paper
• Genetics

  1977cited by this paper
• The hitch-hiking effect of a favourable gene.

  1974cited by this paper
• The hitch-hiking effect of a favourable gene.

  1974cited by this paper
• LSU Digital Commons LSU Digital Commons Chromosomal inversions and the reproductive isolation of Chromosomal inversions and the reproductive isolation of species species

  year unknowncited by this paper

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