Engineering Corynebacterium glutamicum for violacein hyper production

BackgroundCorynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium’s GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications derived from l-tryptophan.ResultsCorynebacterium glutamicum ATCC 21850 that could produce 162.98 mg L−1 tryptophan was employed as a novel host for metabolic engineering chassis. Heterologous vio operon from Chromobacterium violaceum was over-expressed in ATCC 21850 strain with constitutive promoter to have obtained 532 mg L−1 violacein. Considering toxicity of violacein, vio operon was expressed with inducible promoter and 629 mg L−1 violacein was obtained in batch culture. Due to the economical coding nature of vio operon, the compressed RBS of vio genes were replaced with complete strong C. glutamicum ones. And extended expression units were assembled to form a synthetic operon. With this strategy, 1116 mg L−1 violacein in batch culture was achieved. Fermentation process was then optimized by studying induction time, induction concentration, culture composition and fermentation temperature. as a result, a titer of 5436 mg L−1 and a productivity of 47 mg L−1 h−1 were achieved in 3 L bioreactor.ConclusionsWith metabolic engineering and fermentation optimization practice, C. glutamicum 21850 (pEC-C-vio1) was able to produce violacein with both titer and productivity at the highest level ever reported. Due to advantages of mature C. glutamicum fermentation industry, this work has built basis for commercial production of violacein.

• Publication year

  2016

• Venue

  Microbial Cell Factories

• Publication date

  2016-08-24

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1186/s12934-016-0545-0PMID 27557730PMCID 4997675
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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