Taxol-induced polymerization of purified tubulin. Mechanism of action.

Incubation of tubulin with taxol resulted in the assembly of tubulin in the absence of microtubule associated proteins. Optimum assembly occurred at approximately equal concentrations of tubulin and taxol. Both podophyllotoxin and colchicine inhibited the taxol-induced tubulin polymerization. Microtubules formed with taxol were also resistant to podophyllotoxin-induced depolymerization. The rate of tubulin subunit exchange into taxol-induced microtubules at steady state was 5-fold lower than into microtubule-associated protein (MAP2)-stimulated microtubules. There was a marked difference in the kinetics of tubulin polymerized in the presence of both taxol and MAP2 as compared to that obtained with either of them alone. In binding experiments, no competition was observed between taxol and MAP2 or the anti-tubulin drugs, e.g. colchicine, podophyllotoxin, and vinblastine.

• Publication year

  1981

• Venue

  Journal of Biological Chemistry

• Publication date

  1981-10-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(19)68639-4PMID 6116707
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Evidence that microtubule depolymerization early in the cell cycle is sufficient to initiate DNA synthesis.

  1981cited by this paper
• Role of GTP hydrolysis in microtubule treadmilling and assembly.

  1981cited by this paper
• In vitro microtubule assembly regulation by divalent cations and nucleotides.

  1981cited by this paper
• Preferential action of a brain detyrosinolating carboxypeptidase on polymerized tubulin.

  1981cited by this paper
• The effect of taxol on living eggs of Xenopus laevis.

  1980cited by this paper
• Guanasone 5'-(alpha,beta-methylene)triphosphate enhances specifically microtubule nucleation and stops the treadmill of tubulin protomers.

  1980cited by this paper
• Evidence for two growth steps in microtubule polymerization.

  1980cited by this paper
• Taxol stabilizes microtubules in mouse fibroblast cells.

  1980cited by this paper
• Incorporation of the high-molecular-weight microtubule-associated protein 2 (MAP2) into microtubules at steady state in vitro.

  1980cited by this paper
• Isolation of bovine brain microtubule protein without glycerol: polymerization kinetics change during purification cycles.

  1979cited by this paper
• Separation of tubulin from microtubule-associated proteins on phosphocellulose. Accompanying alterations in concentrations of buffer components.

  1979cited by this paper
• Promotion of microtubule assembly in vitro by taxol

  1979cited by this paper
• Competitive inhibition of colchicine binding to tubulin by microtubule-associated proteins.

  1979cited by this paper
• The periodic association of MAP2 with brain microtubules in vitro

  1979cited by this paper
• Opposite end assembly and disassembly of microtubules at steady state in vitro.

  1978cited by this paper
• In vitro assembly of pure tubulin into microtubules in the absence of microtubule-associated proteins and glycerol.

  1977cited by this paper
• Kinetic analysis of microtubule self-assembly in vitro.

  1977cited by this paper
• Podophyllotoxin as a probe for the colchicine binding site of tubulin.

  1977cited by this paper
• Role of tubulin-associated proteins in microtubule nucleation and elongation.

  1977cited by this paper
• Brain tubulin polymerization in the absence of "microtubule-associated proteins".

  1976cited by this paper
• Polycation-induced assembly of purified tubulin.

  1976cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• A protein factor essential for microtubule assembly.

  1975cited by this paper
• A REEXAMINATION OF THE REACTION BETWEEN COLCHICINE AND TUBULIN *

  1975cited by this paper
• Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.

  1975cited by this paper
• BIOCHEMICAL STUDIES ON THE IN VITRO ASSEMBLY AND DISASSEMBLY OF MICROTUBULES *

  1975cited by this paper
• MICROTUBULES AS DRUG RECEPTORS: PHARMACOLOGICAL PROPERTIES OF MICROTUBULE PROTEIN *

  1975cited by this paper
• Properties of rat brain tubulin.

  1974cited by this paper
• Interaction of drugs with microtubule proteins.

  1974cited by this paper
• Microtubule Assembly and Function in Chlamydomonas: Inhibition of Growth and Flagellar Regeneration by Antitubulins and Other Drugs and Isolation of Resistant Mutants

  1974cited by this paper
• THE MECHANISM OF ACTION OF COLCHICINE

  1973cited by this paper
• Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

  1972cited by this paper
• Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia.

  1971cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Spinal overexpression of CAPN1 in CaMKII neurons mediates paclitaxel-induced neuropathic pain via NCS-1-TRPV4 signaling

  2026cites this paper
• From Screening to a Nanotechnological Platform: Cannabidiol–Chemotherapy Co-Loaded Lipid Nanocapsules for Glioblastoma Multiforme Treatment

  2025cites this paper
• Spearmint targets microtubules by (−)-carvone

  2024cites this paper
• DRGAT: Predicting Drug Responses Via Diffusion-Based Graph Attention Network

  2024cites this paper
• Safe, simple and multifunctional hydroxyapatite nanoparticles for efficient overcoming of tumor multidrug resistance

  2024cites this paper
• CEP41, a ciliopathy-linked centrosomal protein, regulates microtubule assembly and cell proliferation

  2024cites this paper
• Chemoproteomics reveals arctigenin as a phagophore-closure blocker via targeting ESCRT-I subunit VPS28.

  2023cites this paper
• Tau Lysine Pseudomethylation Regulates Microtubule Binding and Enhances Prion-like Tau Aggregation

  2023cites this paper
• Modelling microtubule dynamic instability: Microtubule growth, shortening and pause.

  2022cites this paper
• Plasmodium falciparum CRK4 links early mitotic events to the onset of S-phase during schizogony

  2022cites this paper
• Cellular Pathogenesis of Chemotherapy-Induced Peripheral Neuropathy: Insights From Drosophila and Human-Engineered Skin Models

  2022cites this paper
• Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics.

  2022cites this paper
• Spectrin: an alternate target for cytoskeletal drugs

  2022cites this paper
• Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel.

  2022cites this paper
• Paclitaxel exposure-toxicity analysis reveals a pharmacokinetic determinant for dose-limiting neutropenia in East-Asian solid tumor patients: results from two prospective, phase II studies

  2022cites this paper
• The Role of Microtubules in Pancreatic Cancer: Therapeutic Progress

  2021cites this paper
• Synthesis of Indofulvin Pseudo‐Natural Products Yields a New Autophagy Inhibitor Chemotype

  2021cites this paper
• Super.FELT: supervised feature extraction learning using triplet loss for drug response prediction with multi-omics data

  2021cites this paper
• Ni(ii), Cu(ii) and Zn(ii) complexes with the 1-trifluoroethoxyl-2,9,10-trimethoxy-7-oxoaporphine ligand simultaneously target microtubules and mitochondria for cancer therapy

  2021cites this paper
• Characterization of a recently synthesized microtubule-targeting compound that disrupts mitotic spindle poles in human cells

  2021cites this paper
• Ion channels as key partners of cytoskeleton in cancer disease.

  2021cites this paper
• A novel compound that disrupts mitotic spindle poles in human cells

  2020cites this paper
• Comparison of microtubules stabilized with the anticancer drugs cevipabulin and paclitaxel

  2020cites this paper
• Microtubule attachment and centromeric tension shape the protein architecture of the human kinetochore

  2020cites this paper
• Conformational change and GTPase activity of human tubulin: A comparative study on Alzheimer’s disease and healthy brain

  2020cites this paper
• Interplay between Convective and Viscoelastic Forces Controls the Morphology of In Vitro Paclitaxel-Stabilized Microtubules

  2020cites this paper
• When Taxol met tubulin

  2020cites this paper
• N‐end rule pathway inhibitor sensitizes cancer cells to antineoplastic agents by regulating XIAP and RAD21 protein expression

  2020cites this paper
• Effect of Xiao-Ai-Ping injection on paclitaxel pharmacokinetics in rats by LC-MS/MS method.

  2019cites this paper
• Structural Basis and Mechanism for Vindoline Dimers Interacting with α,β-Tubulin

  2019cites this paper
• Effect of monosodium glutamate on testicular tissue of paclitaxel-treated mice: An experimental study

  2019cites this paper
• A Subset of New Platinum Antitumor Agents Kills Cells by a Multimodal Mechanism of Action Also Involving Changes in the Organization of the Microtubule Cytoskeleton.

  2019cites this paper
• High-Dimensional Precision Medicine From Patient-Derived Xenografts

  2019cites this paper
• Preparation and evaluation of carboxymethyl chitosan-rhein polymeric micelles with synergistic antitumor effect for oral delivery of paclitaxel.

  2019cites this paper
• Predicting Paclitaxel Disposition in Humans With Whole‐Body Physiologically‐Based Pharmacokinetic Modeling

  2019cites this paper
• Chemokine‐triggered microtubule polymerization promotes neutrophil chemotaxis and invasion but not transendothelial migration

  2019cites this paper
• Manifestations of Drug Toxicity on Mitochondria in the Nervous System

  2018cites this paper
• A tripartite mode of action approach for investigating the impact of aneugens on tubulin polymerization

  2018cites this paper
• Effects of Fucoidan and Chemotherapeutic Agent Combinations on Malignant and Non-malignant Breast Cell Lines.

  2017cites this paper
• Effects of Adamantyl Derivatives on Pharmacokinetic Behavior of Paclitaxel in Rats

  2017cites this paper
• Synergetic effects of Docetaxel and ionizing radiation reduced cell viability on MCF-7 breast cancer cell

  2017cites this paper
• Bioprobes at a Glance

  2017cites this paper
• Emerging treatment using tubulin inhibitors in advanced non-small cell lung cancer

  2017cites this paper
• Identification of licofelone through three-dimensional cell culture drug screening as a repurposed agent able to reverse drug resistant and cancer stem cell-like subpopulations in ovarian cancer

  2017cites this paper
• Connecting macroscopic dynamics with microscopic properties in active microtubule network contraction

  2017cites this paper
• The Interference of Selected Cytotoxic Alkaloids with the Cytoskeleton: An Insight into Their Modes of Action

  2016cites this paper
• Manuscripts under preparation : ( 1 ) Development of in vivo drug resistant tumor in ordinary laboratory mice

  2016cites this paper
• An interdisciplinary computational/experimental approach to evaluate drug-loaded gold nanoparticle tumor cytotoxicity.

  2016cites this paper
• Exosomes-mediate microRNAs transfer in breast cancer chemoresistance regulation.

  2016cites this paper
• PEG-DERIVATIZED FTS-BASED MICELLES FOR COMBINATION CANCER THERAPY

  2016cites this paper
• Structural investigations into functional aspects of the centriolar key proteins CEP135/Bld10p and SAS-6/Bld12p

  2016cites this paper
• Biomolekulare Mechanismen der Ras-gesteuerten Apoptose

  2016cites this paper
• Role of paclitaxel on critical nucleation concentration of tubulin and its effects thereof.

  2016cites this paper
• Exploring mTOR signalling as a targeted approach against ovarian cancer

  2016cites this paper
• Gender differences in a mouse model of chemotherapy-induced neuropathic pain

  2016cites this paper
• Theoretical insight into the structural mechanism for the binding of vinblastine with tubulin

  2015cites this paper
• Characterisation of CTR-17 and CTR-20, novel chalcone derivatives that inhibit tubulin polymerisation activity

  2015cites this paper
• Mitochondrial Dysfunction in Chemotherapy-Induced Peripheral Neuropathy (CIPN)

  2015cites this paper
• Optimizing and predicting the in vivo activity of AT9283 as a monotherapy and in combination with paclitaxel

  2015cites this paper
• Prognostic significance of autocrine motility factor receptor expression by colorectal cancer and lymph node metastases.

  2015cites this paper
• Sequencing current therapies in the treatment of metastatic prostate cancer.

  2015cites this paper
• Local delivery of paclitaxel in the treatment of peripheral arterial disease

  2015cites this paper
• THE INHIBITORY EFFECT OF PACLITAXEL ON (Kv2.1) K+ CURRENT IN H9c2 CELLS.

  2015cites this paper
• A First-Time-In-Human Phase I Clinical Trial of Bispecific Antibody-Targeted, Paclitaxel-Packaged Bacterial Minicells

  2015cites this paper
• In vivo and in vitro properties of STX2484: a novel non-steroidal anti-cancer compound active in taxane-resistant breast cancer

  2014cites this paper
• PEG-Farnesyl Thiosalicylic Acid Telodendrimer Micelles as an Improved Formulation for Targeted Delivery of Paclitaxel

  2014cites this paper
• New targeted therapies for breast cancer: A focus on tumor microenvironmental signals and chemoresistant breast cancers.

  2014cites this paper
• Aptamers as Both Drugs and Drug-Carriers

  2014cites this paper
• Rapid assessment of drug response in cancer cells using microwell array and molecular imaging

  2014cites this paper
• Amphiphilic carboxymethyl chitosan-quercetin conjugate with P-gp inhibitory properties for oral delivery of paclitaxel.

  2014cites this paper
• In vivo and in vitro properties of STX 2484 : a novel non-steroidal anti-cancer compound active in taxane-resistant breast cancer

  2014cites this paper
• Isolation and Characterisation of Bioactive Secondary Metabolites from Arctic, Marine Organisms

  2014cites this paper
• Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene

  2014cites this paper
• Study of novel nanoparticle transport and drug release for cancer treatment.

  2014cites this paper
• Tanshinone IIA Induces Apoptosis in Human Oral Cancer KB Cells through a Mitochondria-Dependent Pathway

  2014cites this paper
• Natural molecules as tumour inhibitors: Promises and prospects

  2014cites this paper
• Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays.

  2014cites this paper
• Design and synthesis of tubulin-binding agents, and their incorporation into novel dual-acting hybrid molecules targeting the tumour vasculature

  2013cites this paper
• Unprecedented inhibition of tubulin polymerization directed by gold nanoparticles inducing cell cycle arrest and apoptosis.

  2013cites this paper
• Heparin-conjugated pluronic nanogels as multi-drug nanocarriers for combination chemotherapy.

  2013cites this paper
• Antibodies and Measurement of Taxol in Serum Characterization of Polyclonal and Monoclonal Anti-taxol Updated Version Citing Articles E-mail Alerts Characterization of Polyclonal and Monoclonal Anti-taxol Antibodies and Measurement of Taxol in Serum1 Abstract Anti-taxol Antibodies Were Generated in

  2013cites this paper
• LZTS1 downregulation confers paclitaxel resistance and is associated with worse prognosis in breast cancer

  2013cites this paper
• The Subcellular Localisation of HAX1 Isoforms and Their Roles in Cancer Cell Migration, Autophagy and Apoptosis.

  2013cites this paper
• Overexpression of cyclin B1 antagonizes chemotherapeutic-induced apoptosis through PTEN/Akt pathway in human esophageal squamous cell carcinoma cells

  2013cites this paper
• Synergistic effect of paclitaxel and epigenetic agent phenethyl isothiocyanate on growth inhibition, cell cycle arrest and apoptosis in breast cancer cells

  2013cites this paper
• Antimitotic herbicides bind to an unidentified site on malarial parasite tubulin and block development of liver-stage Plasmodium parasites.

  2013cites this paper
• Design and synthesis of cyclopropylamide analogues of combretastatin-A4 as novel microtubule-stabilizing agents.

  2013cites this paper
• Synthesis of N(4)-(substituted phenyl)-N(4)-alkyl/desalkyl-9H-pyrimido[4,5-b]indole-2,4-diamines and identification of new microtubule disrupting compounds that are effective against multidrug resistant cells.

  2013cites this paper
• Substituted 1,6-diphenylnaphthalenes as FtsZ-targeting antibacterial agents.

  2013cites this paper
• Molecular dynamic simulations of the tubulin-human gamma synuclein complex: structural insight into the regulatory mechanism involved in inducing resistance against Taxol.

  2013cites this paper
• Preparation and characterization of novel poly(ethylene glycol) paclitaxel derivatives.

  2013cites this paper
• Avaliação da resistência de células-tronco derivadas de tecido adiposo humano a quimioterápicos

  2012cites this paper
• Biologic effects of SMF and paclitaxel on K562 human leukemia cells.

  2012cites this paper
• Effects of silymarin and formulation on the oral bioavailability of paclitaxel in rats.

  2012cites this paper
• Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene.

  2012cites this paper
• Microtubules as a Target in Cancer Therapy

  2012cites this paper
• Biotechnologically produced secondary plant metabolites for cancer treatment and prevention.

  2012cites this paper
• Alternol exerts prostate‐selective antitumor effects through modulations of the AMPK signaling pathway

  2012cites this paper
• Two different docetaxel resistant MCF-7 sublines exhibited different gene expression pattern

  2012cites this paper
• Enhanced oral bioavailability and anti‐tumour effect of paclitaxel by 20(s)‐ginsenoside Rg3 in vivo

  2012cites this paper