Enzymatic phosphorylation of acetate.

The metabolic utilization of acetate (oxidation via the citric acid cycle, synthesis of higher fatty acids, cholesterol, and possibly other compounds) requires a preliminary activation through conversion to acetyl C0A.l Two main mechanisms are known to bring about this activation (2). One of these mechanisms, utilized by animal tissues, yeast, and some bacteria, seems to involve a single enzyme, usually referred to as the acetate activating enzyme, catalyzing the reversible conversion of ATP, acetate, and CoA to AMP, PP, and acetyl CoA (3-5). The other mechanism, which appears to be restricted to bacteria,2 involves two enzymes, acetokinase (7) and transacetylase (8), catalyzing Reactions 1 and 2 respectively. The net result is Reaction 3. A reversible reaction between ATP and acetate was first described by Lipmann in extracts of La&bacillus delbrueckii (9) and later by Stadtman and Barker in extracts of Clostridium kluyveri (10). The formation of

• Publication year

  1954

• Venue

  Journal of Biological Chemistry

• Publication date

  1954-12-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0021-9258(18)71161-7PMID 13221579
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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