Structure and properties of a human non-pancreatic phospholipase A2.

We have purified a human non-pancreatic phospholipase A2 that is present in platelets and is enriched in rheumatoid synovial fluid. The enzyme is calcium-dependent, has a pH optimum of 8-10, and shows a striking preference for substrate presented in the form of Escherichia coli membranes. In the E. coli phospholipase A2 assay the phospholipase exhibits an apparent specific activity of 300 mumol/mg/min. Using oligonucleotide probes based on amino-terminal sequence data, we cloned the corresponding human gene from a genomic DNA library and expressed the gene in animal cells. The protein was secreted from the cells in an active form. The deduced amino acid sequence of the human protein consists of 124 amino acids, contains structural features common to all known phospholipase A2s, and has a half-cystine pattern that is characteristic of the snake venom group II enzymes.

• Publication year

  1989

• Venue

  Journal of Biological Chemistry

• Publication date

  1989-04-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)83616-xPMID 2925633
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Lysophosphatidylcholine: a chemotactic factor for human monocytes and its potential role in atherogenesis.

  1988cited by this paper
• Interleukin 1 amplifies receptor-mediated activation of phospholipase A2 in 3T3 fibroblasts.

  1988cited by this paper
• Receptor-mediated activation of phospholipase A2 via GTP-binding proteins: arachidonic acid and its metabolites as second messengers.

  1988cited by this paper
• Pathogenesis of hypotension in septic shock: correlation of circulating phospholipase A2 levels with circulatory collapse.

  1988cited by this paper
• Biology of interleukin 1

  1988cited by this paper
• Localization of phospholipase A2 in normal and ras-transformed cells

  1988cited by this paper
• Purification and characterization of membrane-bound phospholipase A2 from rat platelets.

  1988cited by this paper
• Purification and characterization of a membrane-associated phospholipase A2 from rat spleen. Its comparison with a cytosolic phospholipase A2 S-1.

  1988cited by this paper
• A pancreatic-type phospholipase A2 in rat gastric mucosa.

  1988cited by this paper
• A phospholipase A2 in the supernatant fraction of rat spleen. Its similarity to rat pancreatic phospholipase A2.

  1988cited by this paper
• Extracellular phospholipase A2: causative agent in circulatory collapse of septic shock?

  1988cited by this paper
• The chemical basis for interfacial activation of monomeric phospholipases A2. Autocatalytic derivatization of the enzyme by acyl transfer from substrate.

  1988cited by this paper
• Phospholipase A2: function and pharmacological regulation.

  1987cited by this paper
• Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

  1987cited by this paper
• Amino acid composition and NH2-terminal amino acid sequence of rat platelet secretory phospholipase A2.

  1987cited by this paper
• Purification and characterization of extracellular phospholipase A2 from peritoneal cavity of caseinate-treated rat.

  1987cited by this paper
• Facing up to membranes: structure/function relationships in phospholipases.

  1987cited by this paper
• Structure-function relationships of phospholipases. The anticoagulant region of phospholipases A2.

  1987cited by this paper
• Selective release of phospholipase A2 and lysophosphatidylserine-specific lysophospholipase from rat platelets.

  1987cited by this paper
• Isolation of the bovine and human genes for Müllerian inhibiting substance and expression of the human gene in animal cells.

  1986cited by this paper
• Role of secretory phospholipases A2 in the pathobiology of disease.

  1986cited by this paper
• Purification and partial sequence analysis of a 37-kDa protein that inhibits phospholipase A2 activity from rat peritoneal exudates.

  1986cited by this paper
• Identification and purification of sheep platelet phospholipase A2 isoforms. Activation by physiologic concentrations of calcium ion.

  1986cited by this paper
• Pancreatic Phospholipase A2: Isolation of the Human Gene and cDNAs from Porcine Pancreas and Human Lung

  1986cited by this paper
• Interleukin 1 activates phospholipase A2 in rabbit chondrocytes: a possible signal for IL 1 action.

  1986cited by this paper
• Structural and functional properties of a phospholipase A2 purified from an inflammatory exudate.

  1986cited by this paper
• A comparison of the crystal structures of phospholipase A2 from bovine pancreas and Crotalus atrox venom.

  1985cited by this paper
• Chemical and biochemical aspects of platelet activating factor: A novel class of acetylated ether‐linked choline‐phospholipids

  1985cited by this paper
• "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

  1984cited by this paper
• Purification of intracellular phospholipase A2 from rat spleen supernatant by reverse-phase high-performance liquid chromatography.

  1984cited by this paper
• Lambda replacement vectors carrying polylinker sequences.

  1983cited by this paper
• A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

  1983cited by this paper
• Conformational properties of phospholipases A2. Secondary-structure prediction, circular dichroism and relative interface hydrophobicity.

  1983cited by this paper
• Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary dna gene.

  1982cited by this paper
• Novel intestinal phospholipase A2: purification and some molecular characteristics.

  1982cited by this paper
• How is the level of free arachidonic acid controlled in mammalian cells?

  1982cited by this paper
• The 2.5 A crystal structure of a dimeric phospholipase A2 from the venom of Crotalus atrox.

  1981cited by this paper
• Phospholipase A2 electrophoretic variants in reptile venoms.

  1981cited by this paper
• Structure of bovine pancreatic phospholipase A2 at 1.7A resolution.

  1981cited by this paper
• Structure and function of phospholipase A2.

  1981cited by this paper
• Intracellular phospholipases A.

  1980cited by this paper
• Cytolytic and membrane-perturbing properties of lysophosphatidylcholine.

  1979cited by this paper
• Separation and purification of a potent bactericidal/permeability-increasing protein and a closely associated phospholipase A2 from rabbit polymorphonuclear leukocytes. Observations on their relationship.

  1979cited by this paper
• Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

  1977cited by this paper
• Partial characterization and purification of a rabbit granulocyte factor that increases permeability of Escherichia coli.

  1975cited by this paper
• Mediators of Inflammation

  1974cited by this paper
• Lysolecithin and Cell Fusion

  1970cited by this paper

• Secreted phospholipase A2 regulates intercellular communications by coordinating extracellular phospholipid metabolism

  2025cites this paper
• Screening of insect immune suppressors using a recombinant phospholipase A2 of a lepidopteran insect.

  2024cites this paper
• Four phospholipase A2 genes encoded in the western flower thrips genome and their functional differentiation in mediating development and immunity

  2024cites this paper
• Revisiting the reaction pathways for phospholipid hydrolysis catalyzed by phospholipase A2 with QM/MM methods

  2024cites this paper
• Interactions of small molecule inhibitors with secreted phospholipase A2: A review of the structural data

  2024cites this paper
• The Roles of sPLA2s in Skin Homeostasis and Disease

  2023cites this paper
• Epigenetic and Metabolic Regulation of Macrophages during Gout

  2023cites this paper
• Modulation of immunity by the secreted phospholipase A2 family

  2023cites this paper
• PLA2G2A+ cancer-associated fibroblasts mediate pancreatic cancer immune escape via impeding antitumor immune response of CD8+ cytotoxic T cells.

  2023cites this paper
• scTenifoldXct: A semi-supervised method for predicting cell-cell interactions and mapping cellular communication graphs.

  2023cites this paper
• The interaction of secreted phospholipase A2-IIA with the microbiota alters its lipidome and promotes inflammation

  2022cites this paper
• Evaluation of the Impact of Esterases and Lipases from the Circulatory System against Substrates of Different Lipophilicity

  2022influential citation
• Pla2g2a promotes innate Th2-type immunity lymphocytes to increase B1a cells

  2022cites this paper
• Molecular docking studies of natural and synthetic compounds against human secretory PLA2 in therapeutic intervention of inflammatory diseases and analysis of their pharmacokinetic properties

  2022cites this paper
• Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary

  2022cites this paper
• Role of human group IIA secreted phospholipase A2 in malaria pathophysiology: Insights from a transgenic mouse model.

  2021cites this paper
• Sex-Biased Expression of Pharmacogenes across Human Tissues

  2021cites this paper
• Human Group IIA Phospholipase A2—Three Decades on from Its Discovery

  2021cites this paper
• Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation

  2021cites this paper
• Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

  2021cites this paper
• Role of membrane lipids in colon differentiation unravelled by mass spectrometry imaging: arachidonic acid beyond inflammation

  2020cites this paper
• Overcoming Resistance to Therapies Targeting the MAPK Pathway in BRAF-Mutated Tumours

  2020cites this paper
• Phospholipase Superfamily: Structure, Functions, and Biotechnological Applications

  2020cites this paper
• Structural and Functional Aspects of Targeting the Secreted Human Group IIA Phospholipase A2

  2020cites this paper
• Secretory Phospholipases A2 in Plants

  2019cites this paper
• Phospholipase A Subclasses in Acute Respiratory Distress Syndrome

  2019cites this paper
• Polymorphism of Secretary PLA2G2A gene is Associated with its Serum level in Type2 Diabetes Mellitus Patients in Northern Iran.

  2019cites this paper
• Eicosanoids in carcinogenesis

  2019cites this paper
• Insect prostaglandins and other eicosanoids: From molecular to physiological actions

  2019cites this paper
• Oral Mucosal Immunity and Microbiome

  2019cites this paper
• Phospholipid Remodeling in Physiology and Disease.

  2019cites this paper
• Interactions of the effector ExoU from Pseudomonas aeruginosa with short-chain phosphatidylinositides provide insights into ExoU targeting to host membranes

  2019cites this paper
• Prostaglandins and Other Eicosanoids in Insects: Biosynthesis and Biological Actions

  2019cites this paper
• Roles of secreted phospholipase A2 group IIA in inflammation and host defense.

  2019cites this paper
• A Potential Role of Phospholipase 2 Group IIA (PLA2-IIA) in P. gingivalis-Induced Oral Dysbiosis.

  2019cites this paper
• Respective contribution of cytosolic phospholipase A2α and secreted phospholipase A2 IIA to inflammation and eicosanoid production in arthritis.

  2019cites this paper
• Development of a Scalable Method for the Isolation and Purification of Recombinant Secretory Phospholipase A2 Expressed in Methylotrophic Yeast Pichia pastoris

  2019cites this paper
• In silico investigation of the molecular effects caused by R123H variant in secretory phospholipase A2-IIA associated with ARDS.

  2018cites this paper
• Secretory phospholipase A2 group IIA enhances the metabolic rate and increases glucose utilization in response to thyroid hormone

  2018cites this paper
• The need for Group IIA secretory phospholipase A2 (sPLA2-IIA) inhibitors in inflammatory ocular disease treatment

  2018cites this paper
• Group IID, IIE, IIF and III secreted phospholipase A2s☆

  2018cites this paper
• Preparation of the Full Set of Recombinant Mouse- and Human-Secreted Phospholipases A2.

  2017cites this paper
• Review of four major distinct types of human phospholipase A2.

  2017cites this paper
• Molecular dynamics simulations reveal structural insights into inhibitor binding modes and functionality in human Group IIA phospholipase A2

  2017cites this paper
• from Human Lung Macrophages -Glucuronidase Release and IL-6 Production β Induce 2 Secretory Phospholipases A

  2017cites this paper
• Protective effect of quinacrine against glycerol-induced acute kidney injury in rats

  2017cites this paper
• Développement, synthèse et marquage au fluor-18 de ligands de la Lp-PLA2 pour la détection précoce de plaques d’athérome par imagerie TEP.

  2017cites this paper
• Secreted phospholipase A2 and mast cells.

  2015cites this paper
• A new era of secreted phospholipase A2

  2015cites this paper
• Structure‐Based Grafting, Mutation, and Optimization of Peptide Inhibitors to Fit in the Active Pocket of Human Secreted Phospholipase A2: Find New Use of Old Peptide Agents with Anti‐Inflammatory Activity

  2015cites this paper
• Kinetic characterization, optimum conditions for catalysis and substrate preference of secretory phospholipase A2 from Glycine max in model membrane systems.

  2015cites this paper
• Efeitos da MT-I, uma fosfolipase A2, isolada do veneno de Bothrops asper em mastócitos: ativação e sinalização intracelular envolvida na desgranulação.

  2015cites this paper
• Secreted phospholipase A2 is increased in meconium-stained amniotic fluid of term gestations: potential implications for the genesis of meconium aspiration syndrome

  2014cites this paper
• Platelet microparticles are not created equal.

  2014cites this paper
• Platelets release mitochondria serving as substrate for bactericidal group IIA-secreted phospholipase A2 to promote inflammation.

  2014cites this paper
• Epithelium specific ETS transcription factor, ESE-3, of Protobothrops flavoviridis snake venom gland transactivates the promoters of venom phospholipase A2 isozyme genes.

  2014cites this paper
• Early detection of severe sepsis in the emergency room in adults - Clinical utility of prognostic markers

  2014cites this paper
• Molecular mechanisms of inflammation – a central role for cytosolic phospholipase A2

  2014cites this paper
• Serum Phospholipase A2-IIA, hs-CRP, and Lipids in Women With Subclinical Hypothyroidism

  2014cites this paper
• Discovery of Novel Secretory Phospholipase A2 Inhibitors Using Virtual Screen

  2014cites this paper
• Interaction of human synovial phospholipase A2 with mixed lipid bilayers: a coarse-grain and all-atom molecular dynamics simulation study.

  2013cites this paper
• Purification, characterization and bactericidal activities of phospholipase A₂ from the dromedary intestine.

  2013cites this paper
• Inhibition of sPLA2-IIA Prevents LPS-Induced Neuroinflammation by Suppressing ERK1/2-cPLA2α Pathway in Mice Cerebral Cortex

  2013cites this paper
• Effects of Toll-like Receptor Agonists and Tumor Necrosis Factor-α in the SW982 Cell Model for Synovitis

  2013cites this paper
• neutrophils during sepsis Elevated cytosolic phospholipase A2 expression and activity in human

  2013cites this paper
• Purification and characterization of a phospholipase A2-IIA from common stingray (Dasyatis pastinaca) intestine.

  2013cites this paper
• Phospholipases of Mineralization Competent Cells and Matrix Vesicles: Roles in Physiological and Pathological Mineralizations

  2013cites this paper
• Phospholipase A2 inhibitors for the treatment of inflammatory diseases: a patent review (2010 – present)

  2013cites this paper
• Design, Synthesis, and Biological Evaluation of Novel Dual Inhibitors of Secretory Phospholipase A2 and Sphingomyelin Synthase

  2013cites this paper
• A thermoactive secreted phospholipase A₂ purified from the venom glands of Scorpio maurus: relation between the kinetic properties and the hemolytic activity.

  2013cites this paper
• The prognostic and predictive value of selected biomarkers in colorectal cancer

  2013cites this paper
• Phospholipase A2 purification and characterization: a case study.

  2012cites this paper
• Etude de la signalisation calcique dans les cellules gustatives lipidiques chez la souris

  2012cites this paper
• Once initiated, how does toxic tissue injury expand?

  2012cites this paper
• Route and Type of Nutrition and Surgical Stress Influence Secretory Phospholipase A2 (sPLA2) Secretion of the Murine Small Intestine

  2012cites this paper
• Matrix vesicles: structure, composition, formation and function in calcification.

  2011cites this paper
• Purification and biochemical characterization of a secreted group IIA chicken intestinal phospholipase A2

  2011influential citation
• Recent progress in phospholipase A₂ research: from cells to animals to humans.

  2011cites this paper
• Recent Progress on Phospholipases: Different Sources, Assay Methods, Industrial Potential and Pathogenicity

  2011cites this paper
• Inhibition of secreted phospholipases A₂ by 2-oxoamides based on α-amino acids: Synthesis, in vitro evaluation and molecular docking calculations.

  2011cites this paper
• Route and type of nutrition and surgical stress influence secretory phospholipase A2 secretion of the murine small intestine.

  2011cites this paper
• Secretory phospholipase A2 activity toward diverse substrates.

  2011cites this paper
• Active compound from the leaves of Vitex negundo L. shows anti-inflammatory activity with evidence of inhibition for secretory Phospholipase A2 through molecular docking

  2011cites this paper
• Novel phospholipases A of Pseudomonas aeruginosa: biochemical characterisation and cellular localisation

  2011cites this paper
• Secreted phospholipase A2 revisited.

  2011cites this paper
• Phospholipase A2 enzymes: physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention.

  2011cites this paper
• Inhibition of Pro-inflammatory Secreted Phospholipase A2 by Extracts from Cynara cardunculus L.

  2010cites this paper
• Design of Group IIA Secreted/Synovial Phospholipase A2 Inhibitors: An Oxadiazolone Derivative Suppresses Chondrocyte Prostaglandin E2 Secretion

  2010cites this paper
• Anticoagulants from Scorpion Venoms

  2010cites this paper
• Group VIA Ca2+-independent phospholipase A2 (iPLA2beta) and its role in beta-cell programmed cell death.

  2010cites this paper
• Emerging roles of secreted phospholipase A2 enzymes: Lessons from transgenic and knockout mice.

  2010cites this paper
• Design of new secreted phospholipase A2 inhibitors based on docking calculations by modifying the pharmacophore segments of the FPL67047XX inhibitor

  2010cites this paper
• CARACTERIZAÇÃO DA INFLAMAÇÃO ARTICULAR INDUZIDA POR FOSFOLIPASE A2 - GRUPO IIA: DETERMINAÇÃO DAS ALTERAÇÕES HISTOPATOLÓGICAS, COMPORTAMENTAIS E

  2010cites this paper
• Group VIA Ca 2þ -independent phospholipase A2 (iPLA2b) and its role in b-cell programmed cell death

  2010cites this paper
• Molecular Docking and 3D-QSAR CoMFA Studies on Indole Inhibitors of GIIA Secreted Phospholipase A2

  2010cites this paper
• Group V secreted phospholipase A2 contributes to LPS‐induced leukocyte recruitment

  2010cites this paper
• Low Molecular Weight Phospholipases A2 in Mammalian Brain and Neural Cells: Roles in Functions and Dysfunctions

  2010cites this paper
• Thrombin and activated protein C inhibit the expression of secretory group IIA phospholipase A(2) in the TNF-alpha-activated endothelial cells by EPCR and PAR-1 dependent mechanisms.

  2010cites this paper
• The antibacterial activity of phospholipase A(2) type IIA is regulated by the cooperative lipid chain melting behavior in Staphylococcus aureus.

  2010cites this paper
• The effect of serum, glucocorticoids, gender and gestational age on the inactivation, synthesis and secretion of surfactant phospholipids by cultured foetal rat type II pneumocytes

  2010cites this paper