Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC–MS/MS

No abstract is available for this paper.

• Publication year

  2010

• Venue

  Nature Protocols

• Publication date

  2010-09-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/nprot.2010.123PMID 21085123
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Addressing Accuracy and Precision Issues in iTRAQ Quantitation*

  2010cited by this paper
• Systems-level dynamic analyses of fate change in murine embryonic stem cells

  2009cited by this paper
• Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis.

  2009cited by this paper
• Quantitative Analysis of Synaptic Phosphorylation and Protein Expression*S

  2008cited by this paper
• Eight-channel iTRAQ Enables Comparison of the Activity of Six Leukemogenic Tyrosine Kinases*S

  2008cited by this paper
• Search for potential markers for prostate cancer diagnosis, prognosis and treatment in clinical tissue specimens using amine-specific isobaric tagging (iTRAQ) with two-dimensional liquid chromatography and tandem mass spectrometry.

  2008cited by this paper
• Quantitative Proteomics Analysis Demonstrates Post-transcriptional Regulation of Embryonic Stem Cell Differentiation to Hematopoiesis*S

  2008cited by this paper
• Protein labeling by iTRAQ: A new tool for quantitative mass spectrometry in proteome research

  2007cited by this paper
• Comparative time-dependent analysis of potential inflammation biomarkers in lymphoma-bearing SJL mice.

  2007cited by this paper
• Differential protein expression in male and female human lumbar cerebrospinal fluid using iTRAQ reagents after abundant protein depletion

  2007cited by this paper
• Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks

  2007cited by this paper
• Quantitative proteomics reveals posttranslational control as a regulatory factor in primary hematopoietic stem cells.

  2006cited by this paper
• Quantitative analysis of acrylamide labeled serum proteins by LC-MS/MS.

  2006cited by this paper
• Time-resolved Mass Spectrometry of Tyrosine Phosphorylation Sites in the Epidermal Growth Factor Receptor Signaling Network Reveals Dynamic Modules*S

  2005cited by this paper
• Assessing the effects of diurnal variation on the composition of human parotid saliva: quantitative analysis of native peptides using iTRAQ reagents.

  2005cited by this paper
• Search for cancer markers from endometrial tissues using differentially labeled tags iTRAQ and cICAT with multidimensional liquid chromatography and tandem mass spectrometry.

  2005cited by this paper
• Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents*S

  2004cited by this paper
• Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS.

  2003cited by this paper
• Properties of 13C-substituted arginine in stable isotope labeling by amino acids in cell culture (SILAC).

  2003cited by this paper
• Protease‐catalyzed incorporation of 18O into peptide fragments and its application for protein sequencing by electrospray and matrix‐assisted laser desorption/ionization mass spectrometry

  1996cited by this paper

• iTRAQ-Based Proteomic Approach to Study Stress Memory Responses in Plants.

  2026cites this paper
• Pleiotropic roles of elongation factor P in Bacillus subtilis physiology revealed by phenotypic and multi-omics analyses

  2026cites this paper
• The Proteomic and Peptidomic Response of Wheat (Triticum aestivum L.) to Drought Stress

  2025influential citation
• CLN7 protein functions at the interface between endolysosomes and stress granules to promote cell survival

  2025cites this paper
• Comparative Physiological and Proteomic Analyses of Taxillus chinensis Seeds Under Chilling Stress

  2025cites this paper
• Cracking the Kinase Code: Urinary Biomarkers as Early Alarms for AAA Rupture—A Pilot Study

  2025influential citation
• EVC protein regulates Sonic hedgehog signaling during human intervertebral disc development and degeneration

  2025cites this paper
• One-Step Enrichment and Quantitative Analysis of In Vivo Protein Complexes via Dimethylpiperidine Cross-Linker DPST.

  2025cites this paper
• Comparative Label-Based Proteomics of Venoms from Echis ocellatus, Naja nigricollis, and Bitis arietans

  2025cites this paper
• Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome

  2025cites this paper
• Phosphoproteomics profiling of sorafenib-resistant hepatocellular carcinoma patient-derived xenografts reveals potential therapeutic strategies

  2024cites this paper
• Dysregulation of extracellular vesicle protein cargo in female myalgic encephalomyelitis/chronic fatigue syndrome cases and sedentary controls in response to maximal exercise

  2024cites this paper
• Shaping the future of oral cancer diagnosis: advances in salivary proteomics

  2024cites this paper
• Influence of the transcription factor ABI5 on growth and development in Arabidopsis.

  2024cites this paper
• An efficient, amine-specific iTRAQ labeling method improves the peptide and protein identification rates.

  2024cites this paper
• ANXA6 expression as a potential indicator of tumor diagnosis, metastasis and immunity in nasopharyngeal carcinoma.

  2024cites this paper
• Vinculin Identified as a Potential Biomarker in Hand-Arm Vibration Syndrome Based on iTRAQ and LC–MS/MS-Based Proteomic Analysis

  2023cites this paper
• Specific functions of single pistil S-RNases in S-gene homozygous Pyrus germplasm

  2023cites this paper
• Mutant Bisexual and Wild Male Flowers Were Compared by Integrated Proteome and Transcriptome Analyses to Provide Insight into the Sex Conversion of Idesia polycarpa Maxim

  2023cites this paper
• iTRAQ-based proteomic analysis provides novel insight into the postnatal testicular development of Hu sheep.

  2023cites this paper
• Tandem mass tag-based quantitative proteomics analysis reveals the new regulatory mechanism of progranulin in influenza virus infection

  2023cites this paper
• Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma

  2023influential citation
• Dysregulation of extracellular vesicle protein cargo in female ME/CFS cases and sedentary controls in response to maximal exercise

  2023cites this paper
• iTRAQ-based proteomic analysis of heteromorphic leaves reveals eco-adaptability of Populus euphratica Oliv.

  2022cites this paper
• SWATH-MS Proteomic Approach to Discover Novel Protein Targets and Pathways in Response to Abscisic Acid.

  2022cites this paper
• Isotopic Tracer Techniques in Preclinical Research

  2022cites this paper
• Combined Transcriptomic and Proteomic Profiling of E. coli under Microaerobic versus Aerobic Conditions: The Multifaceted Roles of Noncoding Small RNAs and Oxygen-Dependent Sensing in Global Gene Expression Control

  2022cites this paper
• Proteomic Analysis Unravels Response and Antioxidation Defense Mechanism of Rice Plants to Copper Oxide Nanoparticles: Comparison with Bulk Particles and Dissolved Cu Ions

  2022cites this paper
• Proteomic analysis, purification and characterization of a new milk-clotting protease from Tenebrio molitor larvae

  2022cites this paper
• Cinnamaldehyde Resist Salmonella Typhimurium Adhesion by Inhibiting Type I Fimbriae

  2022cites this paper
• Revealing the difference of α-amylase and CYP6AE76 gene between polyphagous Conogethes punctiferalis and oligophagous C. pinicolalis by multiple-omics and molecular biological technique

  2022cites this paper
• Heterozygous LRP1 deficiency causes developmental dysplasia of the hip by impairing triradiate chondrocytes differentiation due to inhibition of autophagy

  2022cites this paper
• Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification.

  2022cites this paper
• Maximizing Depth of PTM Coverage: Generating Robust MS Datasets for Computational Prediction Modeling.

  2022cites this paper
• Stem Cells and Proteomics in Biomaterials and Biomedical Applications

  2022cites this paper
• Xyloketal B Reverses Nutritional Hepatic Steatosis, Steatohepatitis, and Liver Fibrosis through Activation of the PPARα/PGC1α Signaling Pathway.

  2022cites this paper
• Mast cell infiltration of the choroid and protease release are early events in age-related macular degeneration associated with genetic risk at both chromosomes 1q32 and 10q26

  2022cites this paper
• CLN7 mutation causes aberrant redistribution of protein isoforms and contributes to Batten disease pathobiology

  2022cites this paper
• Efficiency of Protein Renewal Is Limited by Feed Intake and Not by Protein Lifetime in Aging Caenorhabditis elegans.

  2022cites this paper
• Transcriptome and Proteomic Analysis Reveals Up-Regulation of Innate Immunity-Related Genes Expression in Caprine Herpesvirus 1 Infected Madin Darby Bovine Kidney Cells

  2021influential citation
• Comparative proteomic analysis of parasitic loranthus seeds exposed to dehydration stress

  2021cites this paper
• The Hitchhiker's guide to glycoproteomics

  2021cites this paper
• Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa

  2021cites this paper
• The Screening of Ovarian Candidate Proteomics Biomarkers of Dolang Sheep at Different Physiological Periods Based on iTRAQ Technology

  2021cites this paper
• iTRAQ-based proteomic analysis of sperm reveals candidate proteins that affect the quality of spermatozoa from boars on plateaus

  2021cites this paper
• Comparative proteomic analysis of parasitic loranthus seeds exposed to dehydration stress

  2021influential citation
• Hypericum perforatum L. Regulates Glutathione Redox Stress and Normalizes Ggt1/Anpep Signaling to Alleviate OVX-Induced Kidney Dysfunction

  2021cites this paper
• Quantitative proteomic analysis reveals that serine/threonine kinase is involved in Streptococcus suis virulence and adaption to stress conditions

  2021cites this paper
• A proteomic approach to understand the impact of nodulation on salinity stress response in alfalfa (Medicago sativa L.).

  2021cites this paper
• Quantitative Proteomic Approach Reveals Altered Metabolic Pathways in Response to the Inhibition of Lysine Deacetylases in A549 Cells under Normoxia and Hypoxia

  2021cites this paper
• Regulation of Gut Microbiota Disrupts the Glucocorticoid Receptor Pathway and Inflammation-related Pathways in the Mouse Hippocampus

  2021cites this paper
• Novel Long Noncoding RNA 005620 Induces Epirubicin Resistance in Triple-Negative Breast Cancer by Regulating ITGB1 Expression

  2021cites this paper
• Study on the differential proteomics of rat hippocampal mitochondria during deep hypothermic circulatory arrest

  2021cites this paper
• Insights Into Mechanism of the Naphthalene-Enhanced Biodegradation of Phenanthrene by Pseudomonas sp. SL-6 Based on Omics Analysis

  2021cites this paper
• Quantitative Proteomic Analysis of Synovial Tissue Reveals That Upregulated OLFM4 Aggravates Inflammation in Rheumatoid Arthritis.

  2021cites this paper
• Combinatorial peptides: A library that continuously probes low‐abundance proteins

  2021cites this paper
• Epigenetic inactivation of the autophagy–lysosomal system in appendix in Parkinson’s disease

  2021cites this paper
• An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feed Forward Loop Interactions

  2021cites this paper
• MAP2K6 remodels chromatin and facilitates reprogramming by activating Gatad2b-phosphorylation dependent heterochromatin loosening

  2021cites this paper
• Using iTRAQ-Based Quantitative Proteomics Analysis to Identify Differentially Expressed Proteins Related to Larval Development of Portunus trituberculatus

  2021cites this paper
• Coordination of root growth with root morphology, physiology and defense functions in response to root pruning in Platycladus orientalis

  2021cites this paper
• iTRAQ-based proteomic analysis reveals potential osteogenesis-promoted role of ATM in strontium-incorporated titanium implant.

  2021cites this paper
• iTRAQ-based proteomics implies inflammasome pathway activation in the prefrontal cortex of CSDS mice may influence resilience and susceptibility.

  2020cites this paper
• Complementary transcriptome and proteome analyses provide insight into floral transition in Bamboo (Dendrocalamus latiflorus Munro)

  2020cites this paper
• Quantitative proteomic analysis of MDCK cell adhesion.

  2020cites this paper
• Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer

  2020cites this paper
• Profound Reprogramming towards Stemness in Pancreatic Cancer Cells as Adaptation to AKT Inhibition

  2020cites this paper
• Isobaric tags for relative and absolute quantitation-based proteomics analysis of the effect of ginger oil on bisphenol A-induced breast cancer cell proliferation

  2020cites this paper
• Anatomic characteristics of epididymis based on histology, proteomic, and 3D reconstruction

  2020cites this paper
• Candidate proteins involved in the calyx abscission process of ‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) identified by iTRAQ analysis

  2020cites this paper
• Epithelial cadherin regulates transition between the naïve and primed pluripotent states in mouse embryonic stem cells

  2020influential citation
• Free amino acids in African indigenous vegetables: Analysis with improved hydrophilic interaction ultra-high performance liquid chromatography tandem mass spectrometry and interactive machine learning.

  2020cites this paper
• Integrative transcriptomics and proteomics analysis constructs a new molecular model for ovule abortion in the female-sterile line of Pinus tabuliformis Carr.

  2020cites this paper
• Hepatic Proteomic Changes and Sirt1/AMPK Signaling Activation by Oxymatrine Treatment in Rats With Non-alcoholic Steatosis

  2020cites this paper
• HUMAN PANCREATIC CANCER CELLS UNDERGO PROFOUND METABOLIC REPROGRAMMING TOWARDS CELLULAR STEMNESS AS ADAPTATION TO INHIBITION OF THE AKT PATHWAY

  2020cites this paper
• iTRAQ-based quantitative glutelin proteomic analysis reveals differentially expressed proteins in the physiological metabolism process during endosperm development and their impacts on yield and quality in autotetraploid rice.

  2020cites this paper
• Identification of Urinary Biomarkers for Exercise-Induced Immunosuppression by iTRAQ Proteomics

  2020cites this paper
• V-ATPase blockade reduces renal gluconeogenesis and improves insulin secretion in type 2 diabetic rats

  2020cites this paper
• Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ

  2020influential citation
• iTRAQ-Based Sperm Proteomic Analyses Reveals Candidate Proteins Affecting the Quality of Spermatozoa From Boar on Plateaus

  2020cites this paper
• Proteomic analysis of synovial tissue in rheumatoid arthritis, OLFM4 promotes fibroblast like synoviocytes proliferation in RA

  2020cites this paper
• NDRG1 facilitates the replication and persistence of Kaposi’s sarcoma-associated herpesvirus by interacting with the DNA polymerase clamp PCNA

  2019cites this paper
• Identification of New Antifungal Agents Targeting Chitin Synthesis by a Chemical-Genetic Method

  2019cites this paper
• What is Normalization? The Strategies Employed in Top-Down and Bottom-Up Proteome Analysis Workflows

  2019cites this paper
• New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach

  2019cites this paper
• Determination of Cerebrospinal Fluid Proteome Variations by Isobaric Labeling Coupled with Strong Cation-Exchange Chromatography and Tandem Mass Spectrometry.

  2019cites this paper
• Advancement of multi-target drug discoveries and promising applications in the field of Alzheimer's disease.

  2019cites this paper
• Cognitive dysfunction in diabetic rats is prevented by pyridoxamine treatment. A multidisciplinary investigation

  2019cites this paper
• Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling

  2019cites this paper
• Chronic mild stress leads to aberrant glucose energy metabolism in depressed Macaca fascicularis models.

  2019cites this paper
• Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

  2019cites this paper
• Improving silkworm genome annotation using a proteogenomics approach.

  2019cites this paper
• Excessive backfat of sows at mating promotes oxidative stress and up-regulates mitochondrial-mediated apoptotic pathway in the full-term placenta

  2019cites this paper
• Proteomic Analysis of Breast Cancer Resistance to the Anticancer Drug RH1 Reveals the Importance of Cancer Stem Cells

  2019cites this paper
• The proteome of Medicago truncatula in response to ammonium and urea nutrition reveals the role of membrane proteins and enzymes of root lignification

  2019cites this paper
• Proteomic and phosphoproteomic studies of monocyte recruitment and macrophage polarisation

  2019cites this paper
• Proteomic Assessment of iTRAQ-Based NaoMaiTong in the Treatment of Ischemic Stroke in Rats

  2019cites this paper
• Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress

  2019cites this paper
• Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ

  2019cites this paper
• Proteomic Analyses of Endarterectomized Tissues from Patients with Chronic Thromboembolic Pulmonary Hypertension

  2019cites this paper