Cloning and Characterization of ThreeXenopus Slug Promoters Reveal Direct Regulation by Lef/β-Catenin Signaling*

In amphibians and birds, one of the first steps of neural crest cell (NCC) determination is expression of the transcription factor Slug. This marker has been used to demonstrate that BMP and Wnt molecules play a major role in NCC induction. However, it is unknown whether Slug expression is directly or indirectly regulated by these signals. We report here the cloning and characterization of three Xenopus Slug promoters: that of the Xenopus tropicalis slug gene and those of two Xenopus laevis Slug pseudoalleles. Although the three genes encode proteins with almost identical amino acid sequences and are expressed with similar spatiotemporal patterns, their 5′-flanking regions are quite different. A striking difference is a deletion in the X. tropicalisgene located precisely at the transcription initiation site that results in the X. tropicalis promoter being inefficient inX. laevis. Additionally, we identified two regions common to the three promoters that are necessary and sufficient to drive specific expression in NCCs. Interestingly, one of the common regulatory regions presents a functional Lef/β-catenin-binding site necessary for specific expression. As the Lef·β-catenin complex is a downstream effector of Wnt signaling, these results suggest thatXenopus Slug is a direct target of NCC determination signals.

• Publication year

  2001

• Venue

  Journal of Biological Chemistry

• Publication date

  2001-08-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.M103167200PMID 11402039
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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