Distribution of molecular size within an unfolded state ensemble using small-angle X-ray scattering and pulse field gradient NMR techniques.

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Journal of Molecular Biology

• Publication date

  2002-02-08

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1006/JMBI.2001.5328PMID 11829506
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Intrinsically unstructured proteins.

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  2001influential reference
• Dramatic stabilization of an SH3 domain by a single substitution: roles of the folded and unfolded states.

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  2000influential reference
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  1999influential reference
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  1998cited by this paper
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  1998cited by this paper
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• NMR studies of unfolded states of an SH3 domain in aqueous solution and denaturing conditions.

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