A fully decompressed synthetic bacteriophage øX174 genome assembled and archived in yeast.

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Virology

• Publication date

  2012-12-20

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.1016/j.virol.2012.09.020PMID 23079106
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Evolution and Diversity of the Microviridae Viral Family through a Collection of 81 New Complete Genomes Assembled from Virome Reads

  2012cited by this paper
• Recombination-based DNA assembly and mutagenesis methods for metabolic engineering.

  2012cited by this paper
• Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

  2012cited by this paper
• Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

  2012cited by this paper
• Evolutionary Recovery of a Recombinant Viral Genome

  2012cited by this paper
• Conformational Switch-Defective ϕX174 Internal Scaffolding Proteins Kinetically Trap Assembly Intermediates before Procapsid Formation

  2012cited by this paper
• Chemical Synthesis of Bacteriophage G4

  2011cited by this paper
• Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

  2010cited by this paper
• Virus transfer between fingerpads and fomites

  2010cited by this paper
• Why genes overlap in viruses

  2010cited by this paper
• Experimental evolution of viruses: Microviridae as a model system

  2010cited by this paper
• Automated Design of Synthetic Ribosome Binding Sites to Precisely Control Protein Expression

  2009cited by this paper
• Genome engineering

  2009cited by this paper
• Enzymatic assembly of DNA molecules up to several hundred kilobases

  2009influential reference
• Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

  2008cited by this paper
• Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

  2008cited by this paper
• A unified model explaining the offsets of overlapping and near-overlapping prokaryotic genes.

  2007cited by this paper
• Intracellular Dynamics of Bacteriophage oX174 Gene Expression

  2007cited by this paper
• Origin and evolution of overlapping genes in the family Microviridae.

  2006cited by this paper
• MuPlex: multi-objective multiplex PCR assay design

  2005cited by this paper
• Refactoring bacteriophage T7

  2005influential reference
• Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides

  2003cited by this paper
• A method for plasmid purification directly from yeast.

  2002cited by this paper
• Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template

  2002cited by this paper
• Primer3 on the WWW for general users and for biologist programmers.

  2000cited by this paper
• Cross-functional analysis of the Microviridae internal scaffolding protein.

  1999cited by this paper
• Multifunctional yeast high-copy-number shuttle vectors.

  1992cited by this paper
• Second-site suppressors of a cold-sensitive prohead accessory protein of bacteriophage phi X174.

  1991cited by this paper
• One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.

  1989cited by this paper
• Mechanism of replication of bacteriophage phi X174. XXII. Site-specific mutagenesis of the A* gene reveals that A* protein is not essential for phi X174 DNA replication.

  1987cited by this paper
• Gene K of bacteriophage phi X174 codes for a protein which affects the burst size of phage production

  1985cited by this paper
• Effects of genome size on bacteriophage phi X174 DNA packaging in vitro.

  1985cited by this paper
• Construction of bacteriophage luminal diameterX174 mutants with maximum genome sizes

  1984cited by this paper
• Genetic applications of yeast transformation with linear and gapped plasmids.

  1983cited by this paper
• Genes and regulatory sequences of bacteriophage phi X174.

  1983cited by this paper
• Is bacteriophage φX174 DNA a message from an extraterrestrial intelligence

  1979cited by this paper
• Nucleotide sequence of the F protein coding region of bacteriophage phiX174 and the amino acid sequence of its product.

  1978cited by this paper
• Identification of lysis protein E of bacteriophage phiX174

  1978cited by this paper
• Nucleotide sequence of bacteriophage φX174 DNA

  1977cited by this paper
• Nucleotide sequence of bacteriophage phi X174 DNA.

  1977cited by this paper
• Overlapping genes in bacteriophage phiX174.

  1976cited by this paper
• Overlapping genes in bacteriophage φX174

  1976cited by this paper

• Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications

  2026cites this paper
• The N-Terminal Domain of Tailspike Depolymerases Affects the Replication Efficiency of Synthetic Klebsiella Phages

  2025cites this paper
• Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages

  2025cites this paper
• Assembly and Mutagenesis of Human Coronavirus OC43 Genomes in Yeast via Transformation-Associated Recombination

  2025influential citation
• Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome

  2025cites this paper
• Synthetic and Functional Engineering of Bacteriophages: Approaches for Tailored Bactericidal, Diagnostic, and Delivery Platforms

  2025cites this paper
• Genomic insights into bacteriophages: a new frontier in AMR detection and phage therapy

  2025cites this paper
• Engineered Phages and Engineered and Recombinant Endolysins Against Carbapenem‐Resistant Gram‐Negative Bacteria: A Focused Review on Novel Antibacterial Strategies

  2025cites this paper
• Replacement of the Genomic Scaffold Improves the Replication Efficiency of Synthetic Klebsiella Phages

  2025cites this paper
• Phage engineering strategies to expand host range for controlling antibiotic-resistant pathogens.

  2025cites this paper
• A synthetic cell phage cycle

  2025cites this paper
• Engineering bacteriophages for targeted superbug eradication

  2025cites this paper
• Optimizing phage therapy with artificial intelligence: a perspective

  2025cites this paper
• CRISPR-Cas9 enables efficient genome engineering of the strictly lytic, broad-host-range staphylococcal bacteriophage K

  2024cites this paper
• Engineering Phages to Fight Multidrug-Resistant Bacteria

  2024cites this paper
• The design and engineering of synthetic genomes

  2024cites this paper
• Engineered phages in anti-infection and anti-tumor field: A review.

  2024cites this paper
• Designing a simple and efficient phage biocontainment system using the amber suppressor initiator tRNA

  2024cites this paper
• Engineered bacteriophages: A panacea against pathogenic and drug resistant bacteria

  2024cites this paper
• Living Bacteriophage Engineering for Functional Material Synthesis, Bioanalytical Sensing and Disease Theranostics

  2024cites this paper
• A computational model for bacteriophage ϕX174 gene expression

  2024cites this paper
• The untapped potential of phage model systems as therapeutic agents

  2024cites this paper
• Genetic Engineering and Rebooting of Bacteriophages in L-Form Bacteria.

  2024cites this paper
• Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid.

  2024cites this paper
• Transformation-associated recombination (TAR) cloning and its applications for gene function; genome architecture and evolution; biotechnology and biomedicine

  2023cites this paper
• Synthetic phage and its application in phage therapy.

  2023cites this paper
• Genetic Engineering and Biosynthesis Technology: Keys to Unlocking the Chains of Phage Therapy

  2023influential citation
• The potential of phage model systems as therapeutic agents 3

  2023cites this paper
• Bacteriophage-based biosensors for detection of pathogenic microbes in wastewater.

  2023cites this paper
• Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions

  2023cites this paper
• Advance on Engineering of Bacteriophages by Synthetic Biology

  2023cites this paper
• Bacteriophages as Biotechnological Tools

  2023cites this paper
• Codon-Restrained Method for Both Eliminating and Creating Intragenic Bacterial Promoters.

  2022cites this paper
• A Phage Foundry Framework to Systematically Develop Viral Countermeasures to Combat Antibiotic-Resistant Bacterial Pathogens

  2022cites this paper
• Three Novel Antisense Overlapping Genes in E. coli O157:H7 EDL933

  2022cites this paper
• Creating De Novo Overlapped Genes

  2022cites this paper
• Synthetic engineering and biological containment of bacteriophages

  2022influential citation
• High-throughput approaches to understand and engineer bacteriophages.

  2022cites this paper
• The Synthesis and Assembly of a Truncated Cyanophage Genome and Its Expression in a Heterogenous Host

  2022cites this paper
• Generating dynamic gene expression patterns without the need for regulatory circuits

  2022cites this paper
• Scarless Recombineering of Phage in Lysogenic State.

  2022cites this paper
• Bacteriophage and endolysin engineering for biocontrol of food pathogens/pathogens in the food: recent advances and future trends

  2022cites this paper
• Proteomic and Transcriptomic Analysis of Microviridae φX174 Infection Reveals Broad Upregulation of Host Escherichia coli Membrane Damage and Heat Shock Responses

  2021cites this paper
• Engineered Bacteriophage Therapeutics: Rationale, Challenges and Future

  2021cites this paper
• Plaque Size Tool: an automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements

  2021cites this paper
• The Many Applications of Engineered Bacteriophages—An Overview

  2021cites this paper
• Virus-associated organosulfur metabolism in human and environmental systems

  2021cites this paper
• Genome-scale top-down reduction of phages to generate viable minimal phage genomes

  2021cites this paper
• Overlapping genes in natural and engineered genomes

  2021cites this paper
• Renaissance for Phage-Based Bacterial Control.

  2021cites this paper
• Effectiveness and Fate Nitrogen in Plants Physiology, Molecular Approaches for Maximization and Improving Nitrogen Use Efficiency. A Review

  2021cites this paper
• Comparison of CRISPR and Marker-Based Methods for the Engineering of Phage T7

  2020cites this paper
• Engineered Bacteriophages for Practical Applications.

  2020cites this paper
• Generating complex patterns of gene expression without regulatory circuits

  2020cites this paper
• Evidence for Numerous Embedded Antisense Overlapping Genes in Diverse E. coli Strains

  2020cites this paper
• Mapping the functional landscape of the receptor binding domain of T7 bacteriophage by deep mutational scanning

  2020cites this paper
• Bacteriophage Use in Molecular Biology and Biotechnology

  2020cites this paper
• Proteomic and transcriptomic analysis of Microviridae φXI74 infection reveals broad up-regulation of host membrane damage and heat shock responses

  2020cites this paper
• Enhancing phage therapy through synthetic biology and genome engineering

  2020cites this paper
• Genome Modularization Reveals Overlapped Gene Topology Is Necessary for Efficient Viral Reproduction.

  2020cites this paper
• Genome modularization reveals overlapped gene topology is necessary for efficient viral reproduction

  2020influential citation
• A high-resolution map of bacteriophage ϕX174 transcription.

  2020cites this paper
• Evolution of Epistasis: Small Populations Go Their Separate Ways

  2020cites this paper
• A high-resolution map of bacteriophage øX174 transcription

  2020cites this paper
• Reporter Phage-Based Detection of Bacterial Pathogens: Design Guidelines and Recent Developments

  2020cites this paper
• Engineering Bacteriophages as Versatile Biologics.

  2019cites this paper
• Physiological and genomic characterization of six virulent bacteriophages of Shiga toxin-producing Escherichia coli O157:H7 for biocontrol and detection applications

  2019cites this paper
• Saturation mutagenesis genome engineering of infective ΦX174 bacteriophage via unamplified oligo pools and golden gate assembly

  2019cites this paper
• Finally, a Role Befitting Astar: Strongly Conserved, Unessential Microvirus A* Proteins Ensure the Product Fidelity of Packaging Reactions

  2019cites this paper
• Engineering gene overlaps to sustain genetic constructs in vivo

  2019cites this paper
• Genetic Engineering of Bacteriophages Against Infectious Diseases

  2019cites this paper
• Utilizing in vitro DNA assembly to engineer a synthetic T7 Nanoluc reporter phage for Escherichia coli detection.

  2019cites this paper
• Characterization of the all-E. coli transcription-translation system myTXTL by mass spectrometry.

  2019cites this paper
• Organisms with alternative genetic codes resolve unassigned codons via mistranslation and ribosomal rescue

  2018cites this paper
• Phage-Based Applications in Synthetic Biology.

  2018cites this paper
• Diseño de 3 genes sintéticos y construcción de una cepa de Escherichia coli C como plataforma para la propagación del bacteriófago ФX174 recombinante.

  2018cites this paper
• [Creation of synthetic bacterial viruses].

  2018cites this paper
• Cross-genus rebooting of custom-made, synthetic bacteriophage genomes in L-form bacteria

  2018cites this paper
• Definitive demonstration by synthesis of genome annotation completeness

  2018cites this paper
• Functional Requirements in the Program and the Cell Chassis for Next‐Generation Synthetic Biology

  2018cites this paper
• Coat Protein Mutations That Alter the Flux of Morphogenetic Intermediates through the ϕX174 Early Assembly Pathway

  2017cites this paper
• Genome-wide engineering of an infectious clone of herpes simplex virus type 1 using synthetic genomics assembly methods

  2017cites this paper
• Symbiotic Nitrogen Fixation and the Challenges to Its Extension to Nonlegumes

  2016cites this paper
• Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii.

  2016cites this paper
• Symbiotic Nitrogen Fixation and Challenges to Extending it to Non-Legumes 1 2

  2016cites this paper
• Genetically Engineered Phages: a Review of Advances over the Last Decade

  2016cites this paper
• Phage Therapy in the Era of Synthetic Biology.

  2016cites this paper
• Programming Bacteriophages by Swapping Their Specificity Determinants.

  2015cites this paper
• TIMI 1254 No . of Pages 2 Spotlight Programming Bacteriophages by Swapping Their Speci fi city Determinants Moran [ 1 _ TD $ DIFF ]

  2015cites this paper
• Bacteriophage Diversity in Different Habitats and Their Role in Pathogen Control

  2015cites this paper
• Development and analysis of a mathematical model for a synthetic biological cell

  2015cites this paper
• Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing

  2015cites this paper
• Proof by synthesis of Tobacco mosaic virus

  2014cites this paper
• Omics approaches in food safety: fulfilling the promise?

  2014cites this paper
• Large-scale de novo DNA synthesis: technologies and applications

  2014cites this paper
• Programming biological operating systems: genome design, assembly and activation

  2014cites this paper
• Bacteriophage-based synthetic biology for the study of infectious diseases.

  2014cites this paper
• Precise and reliable gene expression via standard transcription and translation initiation elements

  2013cites this paper
• Biología Sintética: La Próxima Revolución Industrial

  2013cites this paper
• Phi (ϕ)X174, Genetics of

  2013cites this paper